UniProtKB - Q640S2 (CRBN_XENTR)
Protein
Protein cereblon
Gene
crbn
Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Functioni
Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Normal degradation of key regulatory proteins is required for limb outgrowth and expression of the fibroblast growth factor FGF8. May also play a role in the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning.By similarity
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 326 | ZincBy similarity | 1 | |
Metal bindingi | 329 | ZincBy similarity | 1 | |
Metal bindingi | 394 | ZincBy similarity | 1 | |
Metal bindingi | 397 | ZincBy similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
- protein ubiquitination Source: UniProtKB
Keywordsi
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein cereblon |
Gene namesi | Name:crbn |
Organismi | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
Taxonomic identifieri | 8364 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana |
Proteomesi |
|
Organism-specific databases
Xenbasei | XB-GENE-973907 crbn |
Subcellular locationi
Nucleus
- nucleus Source: UniProtKB
Other locations
- Cul4A-RING E3 ubiquitin ligase complex Source: UniProtKB
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076164 | 1 – 447 | Protein cereblonAdd BLAST | 447 |
Proteomic databases
PaxDbi | Q640S2 |
Expressioni
Gene expression databases
Bgeei | ENSXETG00000005146 Expressed in 13 organ(s), highest expression level in skeletal muscle tissue |
Interactioni
Subunit structurei
Component of a DCX (DDB1-CUL4-X-box) protein ligase complex.
By similarityProtein-protein interaction databases
STRINGi | 8364.ENSXETP00000011183 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 82 – 320 | Lon N-terminalPROSITE-ProRule annotationAdd BLAST | 239 | |
Domaini | 321 – 429 | CULTPROSITE-ProRule annotationAdd BLAST | 109 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 381 – 389 | Thalidomide bindingBy similarity | 9 |
Sequence similaritiesi
Belongs to the CRBN family.Curated
Phylogenomic databases
eggNOGi | KOG1400 Eukaryota ENOG410XQGE LUCA |
GeneTreei | ENSGT00390000016404 |
HOGENOMi | HOG000067866 |
InParanoidi | Q640S2 |
KOi | K11793 |
OMAi | CQVYERG |
OrthoDBi | 1069900at2759 |
TreeFami | TF106115 |
Family and domain databases
CDDi | cd15777 CRBN_C_like, 1 hit |
InterProi | View protein in InterPro IPR034750 CULT IPR003111 Lon_substr-bd IPR015947 PUA-like_sf IPR004910 Yippee/Mis18/Cereblon |
Pfami | View protein in Pfam PF02190 LON_substr_bdg, 1 hit PF03226 Yippee-Mis18, 1 hit |
SMARTi | View protein in SMART SM00464 LON, 1 hit |
SUPFAMi | SSF88697 SSF88697, 1 hit |
PROSITEi | View protein in PROSITE PS51788 CULT, 1 hit PS51787 LON_N, 1 hit |
i Sequence
Sequence statusi: Complete.
Q640S2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADDEGEEDP GINNMGNLLQ VISSESEEED EMELEDAKTA DSESPNIINF
60 70 80 90 100
DTSLPTSHAY LGVDMEEFHG RTLHDDDSCQ QIPVLPHVQV MLIPGQTLPL
110 120 130 140 150
HLSRPQEVSM VRGLIQRDRT FAVLAYSDGL QREAHFGTTA EIYAYREEHE
160 170 180 190 200
FGIETVKVKA IGRQRFQVLE TRTQADGIQV ARVQILPERV LPCPMTSLQL
210 220 230 240 250
DSQSRHLLFP TNKPVSGRSP QSKCQWLHKY RRRKFLGASL TSWPSWLYAL
260 270 280 290 300
YDADSLMERV KLQLHEWDEN LRDDSLPANP IDFSYRVAAC LPIDDALRIQ
310 320 330 340 350
LLQIGNAIQR LRCELDIMSK CTSLCCKHCP DTEITTKNEI FSLSLCGPMA
360 370 380 390 400
AYVNPHGYVH ETLTVYKAFN LSLVGRPSTE NSWFPGFAWT IAQCRVCGSH
410 420 430 440
MGWKFTAVRK DLSPQKFWGL TRSALQPRIP EPDEGEMGHD HSPILCL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC082517 mRNA Translation: AAH82517.1 |
RefSeqi | NP_001008192.1, NM_001008191.1 |
Genome annotation databases
Ensembli | ENSXETT00000011183; ENSXETP00000011183; ENSXETG00000005146 |
GeneIDi | 493554 |
KEGGi | xtr:493554 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC082517 mRNA Translation: AAH82517.1 |
RefSeqi | NP_001008192.1, NM_001008191.1 |
3D structure databases
SMRi | Q640S2 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 8364.ENSXETP00000011183 |
Proteomic databases
PaxDbi | Q640S2 |
Genome annotation databases
Ensembli | ENSXETT00000011183; ENSXETP00000011183; ENSXETG00000005146 |
GeneIDi | 493554 |
KEGGi | xtr:493554 |
Organism-specific databases
CTDi | 51185 |
Xenbasei | XB-GENE-973907 crbn |
Phylogenomic databases
eggNOGi | KOG1400 Eukaryota ENOG410XQGE LUCA |
GeneTreei | ENSGT00390000016404 |
HOGENOMi | HOG000067866 |
InParanoidi | Q640S2 |
KOi | K11793 |
OMAi | CQVYERG |
OrthoDBi | 1069900at2759 |
TreeFami | TF106115 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Gene expression databases
Bgeei | ENSXETG00000005146 Expressed in 13 organ(s), highest expression level in skeletal muscle tissue |
Family and domain databases
CDDi | cd15777 CRBN_C_like, 1 hit |
InterProi | View protein in InterPro IPR034750 CULT IPR003111 Lon_substr-bd IPR015947 PUA-like_sf IPR004910 Yippee/Mis18/Cereblon |
Pfami | View protein in Pfam PF02190 LON_substr_bdg, 1 hit PF03226 Yippee-Mis18, 1 hit |
SMARTi | View protein in SMART SM00464 LON, 1 hit |
SUPFAMi | SSF88697 SSF88697, 1 hit |
PROSITEi | View protein in PROSITE PS51788 CULT, 1 hit PS51787 LON_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CRBN_XENTR | |
Accessioni | Q640S2Primary (citable) accession number: Q640S2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 30, 2005 |
Last sequence update: | October 25, 2004 | |
Last modified: | October 16, 2019 | |
This is version 89 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - PATHWAY comments
Index of metabolic and biosynthesis pathways