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Protein

PAN2-PAN3 deadenylation complex subunit Pan3

Gene

Pan3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei467ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri49 – 77C3H1-typeUniRule annotationAdd BLAST29
Nucleotide bindingi516 – 523ATPUniRule annotation8
Nucleotide bindingi590 – 591ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

  • deadenylation-dependent decapping of nuclear-transcribed mRNA Source: MGI
  • mRNA processing Source: UniProtKB-KW
  • nuclear-transcribed mRNA poly(A) tail shortening Source: GO_Central
  • positive regulation of cytoplasmic mRNA processing body assembly Source: MGI
  • protein targeting Source: MGI

Keywordsi

Biological processmRNA processing
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
PAN2-PAN3 deadenylation complex subunit Pan3UniRule annotation
Alternative name(s):
PAB1P-dependent poly(A)-specific ribonucleaseUniRule annotation
Poly(A)-nuclease deadenylation complex subunit 3UniRule annotation
Short name:
PAN deadenylation complex subunit 3UniRule annotation
Gene namesi
Name:Pan3UniRule annotation
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1919837 Pan3

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002805261 – 837PAN2-PAN3 deadenylation complex subunit Pan3Add BLAST837

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei300PhosphoserineBy similarity1
Modified residuei307PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ640Q5
PaxDbiQ640Q5
PeptideAtlasiQ640Q5
PRIDEiQ640Q5

PTM databases

iPTMnetiQ640Q5
PhosphoSitePlusiQ640Q5

Expressioni

Gene expression databases

BgeeiENSMUSG00000029647
ExpressionAtlasiQ640Q5 baseline and differential
GenevisibleiQ640Q5 MM

Interactioni

Subunit structurei

Homodimer. Forms a heterotrimer with a catalytic subunit PAN2 to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts (via PAM-2 motif) with poly(A)-binding protein PABPC1 (via PABC domain), conferring substrate specificity of the enzyme complex. Interacts with the GW182 family proteins TNRC6A, TNRC6B and TNRC6C.UniRule annotation

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031651

Structurei

3D structure databases

ProteinModelPortaliQ640Q5
SMRiQ640Q5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni409 – 696Pseudokinase domainUniRule annotationAdd BLAST288
Regioni736 – 837Knob domainUniRule annotationAdd BLAST102

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili230 – 245UniRule annotationAdd BLAST16

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi74 – 94PABPC-interacting motif-2 (PAM-2)CuratedAdd BLAST21

Domaini

The N-terminal zinc finger binds to poly(A) RNA.UniRule annotation
Contains a pseudokinase domain. The protein kinase domain is predicted to be catalytically inactive because some of the residues important for catalytic activity are substituted and it lacks the equivalent of the binding site for a peptide substrate. However, it has retained an ATP-binding site and ATP-binding is required for mRNA degradation, stimulating the activity of the PAN2 nuclease in vitro. The nucleotide-binding site is juxtaposed to the RNase active site of PAN2 in the complex and may actually bind nucleosides of a poly(A) RNA rather than ATP, feeding the poly(A)-tail to the active site of the deadenylase and thus increasing the efficiency with which this distributive enzyme degrades oligo(A) RNAs.UniRule annotation
The pseudokinase domain, the coiled-coil (CC), and C-terminal knob domain (CK) form a structural unit (PKC) that forms an extensive high-affinity interaction surface for PAN2.UniRule annotation

Sequence similaritiesi

Belongs to the protein kinase superfamily. PAN3 family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri49 – 77C3H1-typeUniRule annotationAdd BLAST29

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG3741 Eukaryota
ENOG410XQ42 LUCA
GeneTreeiENSGT00390000001504
HOGENOMiHOG000007619
HOVERGENiHBG058849
InParanoidiQ640Q5
KOiK12572
TreeFamiTF105865

Family and domain databases

HAMAPiMF_03181 PAN3, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR030844 PAN3
IPR000719 Prot_kinase_dom
IPR000571 Znf_CCCH
IPR036855 Znf_CCCH_sf
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SM00356 ZnF_C3H1, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
SSF90229 SSF90229, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS50103 ZF_C3H1, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q640Q5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSGGGGGLP PPSAAASPSS SSLAAAVAVA VAASSGVGGV PGGPAAAAGV
60 70 80 90 100
KLKYCRYYAK DKTCFYGEEC QFLHEDPAAG AAPGLGLHSN SVPLALAAAA
110 120 130 140 150
GAAFPPGALP GGGAGPPAGP KKPELGVPGA ATAGGGLDGP RVAIPGMDGG
160 170 180 190 200
ALTDASLTES YFSTSFIGVN GFGSPVETKY PLMQRMTSSS SSPSLLNDSA
210 220 230 240 250
KPYTGHDLLT SSASSLFNDF GALNISQRRK TPNPTASEFI PKGGSTSRLS
260 270 280 290 300
NVSQSNMSAF SQVFSHPSMG SPATAGLAPG MSLSAGSSPL HSPKITPHTS
310 320 330 340 350
PAPRRRSHTP NPASFMVPPS ASTPANNPAP QPPSSGQVIQ KETVGGTTYF
360 370 380 390 400
YTDTTPAPLT GMVFPNYHIY PPTAPHVAYM QPKANAPSFF MADELRQELI
410 420 430 440 450
NRHLITMAQI DQADMPAVPT EVDSYHSLFP LEPLPPPNRI QKSSNFGYIT
460 470 480 490 500
SCYKAVNSKD DLPYCLRRIH GFRLVNTKCM VLVDMWKKIQ HSNIVTLREV
510 520 530 540 550
FTTKAFAEPS LVFAYDFHAG GETMMSRHFN DPNSDAYFTK RKWGQHDGPL
560 570 580 590 600
PRQHAGLLPE SLIWAYIVQL SSALRTIHTA GLACRVMDPT KILITSKTRL
610 620 630 640 650
RVNCVGVFDV LTFDNSQNNN PLALMAQYQQ ADLISLGKVV LALACNSLAG
660 670 680 690 700
IQRENLQKAM ELVTINYSSD LKNLILYLLT DQNRMRSVND IMPMIGARFY
710 720 730 740 750
TQLDAAQMRN DVIEEDLAKE VQNGRLFRLL AKLGTINERP EFQKDPTWSE
760 770 780 790 800
TGDRYLLKLF RDHLFHQVTE AGAPWIDLSH IISCLNKLDA GVPEKISLIS
810 820 830
RDEKSVLVVT YSDLKRCFEN TFQELIAAAN GNDRNSN
Length:837
Mass (Da):89,745
Last modified:July 27, 2011 - v3
Checksum:i323CACB0F32E745A
GO
Isoform 2 (identifier: Q640Q5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     281-301: MSLSAGSSPLHSPKITPHTSP → NKLPSESALDLKINPTVHETD
     302-837: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:301
Mass (Da):29,592
Checksum:i92A49BF5ECFF5FEE
GO

Sequence cautioni

The sequence AAH82547 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated
The sequence BAB28221 differs from that shown. Intron retention.Curated
The sequence BAE32127 differs from that shown. Several sequencing errors.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_041650281 – 301MSLSA…PHTSP → NKLPSESALDLKINPTVHET D in isoform 2. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_041651302 – 837Missing in isoform 2. 1 PublicationAdd BLAST536

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012409 mRNA Translation: BAB28221.1 Sequence problems.
AK028869 mRNA Translation: BAC26161.1
AK153632 mRNA Translation: BAE32127.1 Sequence problems.
AC131730 Genomic DNA No translation available.
AC134441 Genomic DNA No translation available.
BC082547 mRNA Translation: AAH82547.1 Sequence problems.
CCDSiCCDS39401.2 [Q640Q5-1]
RefSeqiNP_082567.3, NM_028291.4 [Q640Q5-1]
UniGeneiMm.320469
Mm.491496

Genome annotation databases

EnsembliENSMUST00000031651; ENSMUSP00000031651; ENSMUSG00000029647 [Q640Q5-1]
GeneIDi72587
KEGGimmu:72587
UCSCiuc009aod.2 mouse [Q640Q5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPAN3_MOUSE
AccessioniPrimary (citable) accession number: Q640Q5
Secondary accession number(s): Q3U5F2, Q8CE75, Q9CZM6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 125 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

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