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Protein

ADP-ribosylation factor-like protein 13B

Gene

Arl13b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cilium-specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B. Binds GTP but is not able to hydrolyze it; the GTPase activity remains unclear. Required to pattern the neural tube. Involved in cerebral cortex development: required for the initial formation of a polarized radial glial scaffold, the first step in the construction of the cerebral cortex, by regulating ciliary signaling (PubMed:23817546). Regulates the migration and placement of postmitotic interneurons in the developing cerebral cortex (PubMed:23153492).7 Publications

Miscellaneous

Used as a ciliary marker because of its specific localization to microtubule doublets of the ciliary axoneme. Frequently used to study cilium signaling, since in contrast to most cilia null mutants, deletion of Arl13b impairs without destroying cilia and their downstream pathways (PubMed:23817546).1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 35GTPBy similarity8
Nucleotide bindingi71 – 75GTPBy similarity5
Nucleotide bindingi130 – 133GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5624958 ARL13B-mediated ciliary trafficking of INPP5E

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 13B
Alternative name(s):
ADP-ribosylation factor-like protein 2-like 1
Short name:
ARL2-like protein 1
Protein hennin
Gene namesi
Name:Arl13b
Synonyms:Arl2l1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1915396 Arl13b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Membrane

Pathology & Biotechi

Disruption phenotypei

Impaired cilium's ability to convey critical extracellular signals such as Shh, without destroying cilia and their downstream pathways. Mice show a constitutive low-level of Shh activity owing to loss of modulation of Gli2 activator, corresponding to the specification of progenitors of motoneurons through most of the neural tube. In contrast to other mouse mutants that disrupt cilia, Gli3 repressor activity is unaffected in mutants (PubMed:17488627). Mutants show abnormal cilia in which components of Shh signaling are not regulated properly: Ptch1 and Smo localize to cilia regardless of Shh stimulation, and there is no Gli enrichment in cilia upon Shh stimulation (PubMed:21976698). Conditional deletion disrupts interneuronal placement, but not postmigratory differentiation in the developing cerebral cortex (PubMed:23153492). Early neuroepithelial-specific deletion in cortical progenitors induces a reversal of the apical-basal polarity of radial progenitors and aberrant neuronal placement (PubMed:23817546).7 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi8 – 9CC → SS: Abolishes palmitoylation and localization to the cilium membrane. 1 Publication2
Mutagenesisi358V → A: Abolishes localization to cilium. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002511381 – 427ADP-ribosylation factor-like protein 13BAdd BLAST427

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi8S-palmitoyl cysteine1 Publication1
Lipidationi9S-palmitoyl cysteine1 Publication1
Modified residuei328PhosphoserineCombined sources1

Post-translational modificationi

Sumoylation is required for PKD2 entry into cilium.By similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ640N2
PaxDbiQ640N2
PeptideAtlasiQ640N2
PRIDEiQ640N2

PTM databases

iPTMnetiQ640N2
PhosphoSitePlusiQ640N2
SwissPalmiQ640N2

Expressioni

Tissue specificityi

Cilium-specific protein. Expressed in neuroepithelial cells and developing radial glia of the developing cerebral cortex: present in both neuroepithelial and radial progenitor cells. In radial progenitors, found in the apical, cell soma domains of these cells (at protein level). Expressed in the primary cilia of postmitotic cortical neurons during embryonic and postnatal development.2 Publications

Gene expression databases

BgeeiENSMUSG00000022911 Expressed in 225 organ(s), highest expression level in blastocyst
CleanExiMM_ARL13B
GenevisibleiQ640N2 MM

Interactioni

Subunit structurei

Monomer. Interacts with IFT complex B components IFT46 and IFT74 (By similarity). Interacts with PIFO.By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000086703

Structurei

3D structure databases

ProteinModelPortaliQ640N2
SMRiQ640N2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili194 – 285Sequence analysisAdd BLAST92

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi371 – 379Poly-Pro9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0074 Eukaryota
KOG0076 Eukaryota
ENOG410XRG9 LUCA
GeneTreeiENSGT00550000074639
HOGENOMiHOG000034031
HOVERGENiHBG060221
InParanoidiQ640N2
KOiK07962
OMAiKKGLFWL
OrthoDBiEOG091G0BQV
TreeFamiTF105476

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q640N2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFSLMANCCN LFKRWREPVR KVTLVMVGLD NAGKTATAKG IQGEHPEDVA
60 70 80 90 100
PTVGFSKIDL RQGKFQVTIF DLGGGKRIRG IWKNYYAESY GVIFVVDSSD
110 120 130 140 150
EERMEETKET MSEVLRHPRI SGKPILVLAN KQDKEGALGE ADVIECLSLE
160 170 180 190 200
KLVNEHKCLC QIEPCSAVLG YGKKIDKSIK KGLYWLLHII AKDFDALSER
210 220 230 240 250
IQKDTTEQRA LEEQEKRERA ERVRKLREER EREQTELDGT SGLAEIDSGP
260 270 280 290 300
VLANPFQPIA AVIIENEKKQ EKEKKKQTVE KDSDVGLLEH KVEPEQAAPQ
310 320 330 340 350
SEADCCLQNP DERVVDSYRE ALSQQLDSED EQDQRGSESG ENSKKKTKKL
360 370 380 390 400
RMKRSHRVEP VNTDESTPKS PTPPQPPPPV GWGTPKVTRL PKLEPLGETR
410 420
HNDFYGKPLP PLAVRQRPNG DAQDTIS
Length:427
Mass (Da):48,144
Last modified:October 3, 2012 - v2
Checksum:iAC131D386B999ABB
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A338P6Z3A0A338P6Z3_MOUSE
ADP-ribosylation factor-like protei...
Arl13b
126Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti356H → Q in AAH82574 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC154309 Genomic DNA No translation available.
CH466521 Genomic DNA Translation: EDK98235.1
BC082574 mRNA Translation: AAH82574.1
CCDSiCCDS28261.1
RefSeqiNP_080853.3, NM_026577.4
UniGeneiMm.96833

Genome annotation databases

EnsembliENSMUST00000089289; ENSMUSP00000086703; ENSMUSG00000022911
GeneIDi68146
KEGGimmu:68146
UCSCiuc007zpu.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC154309 Genomic DNA No translation available.
CH466521 Genomic DNA Translation: EDK98235.1
BC082574 mRNA Translation: AAH82574.1
CCDSiCCDS28261.1
RefSeqiNP_080853.3, NM_026577.4
UniGeneiMm.96833

3D structure databases

ProteinModelPortaliQ640N2
SMRiQ640N2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000086703

PTM databases

iPTMnetiQ640N2
PhosphoSitePlusiQ640N2
SwissPalmiQ640N2

Proteomic databases

MaxQBiQ640N2
PaxDbiQ640N2
PeptideAtlasiQ640N2
PRIDEiQ640N2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089289; ENSMUSP00000086703; ENSMUSG00000022911
GeneIDi68146
KEGGimmu:68146
UCSCiuc007zpu.2 mouse

Organism-specific databases

CTDi200894
MGIiMGI:1915396 Arl13b

Phylogenomic databases

eggNOGiKOG0074 Eukaryota
KOG0076 Eukaryota
ENOG410XRG9 LUCA
GeneTreeiENSGT00550000074639
HOGENOMiHOG000034031
HOVERGENiHBG060221
InParanoidiQ640N2
KOiK07962
OMAiKKGLFWL
OrthoDBiEOG091G0BQV
TreeFamiTF105476

Enzyme and pathway databases

ReactomeiR-MMU-5624958 ARL13B-mediated ciliary trafficking of INPP5E

Miscellaneous databases

ChiTaRSiArl13b mouse
PROiPR:Q640N2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022911 Expressed in 225 organ(s), highest expression level in blastocyst
CleanExiMM_ARL13B
GenevisibleiQ640N2 MM

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAR13B_MOUSE
AccessioniPrimary (citable) accession number: Q640N2
Secondary accession number(s): G3X9K5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 3, 2012
Last modified: November 7, 2018
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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