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Protein

Guanine nucleotide exchange factor DBS

Gene

Mcf2l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor that catalyzes guanine nucleotide exchange on RHOA and CDC42, and thereby contributes to the regulation of RHOA and CDC42 signaling pathways (PubMed:17000758, PubMed:11889037, PubMed:12006984). Seems to lack activity with RAC1. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor
LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide exchange factor DBS
Alternative name(s):
DBL's big sister
MCF2-transforming sequence-like protein
Gene namesi
Name:Mcf2l
Synonyms:Dbs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:103263 Mcf2l

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000809361 – 1149Guanine nucleotide exchange factor DBSAdd BLAST1149

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei457PhosphoserineBy similarity1
Modified residuei462PhosphoserineBy similarity1
Modified residuei471PhosphoserineBy similarity1
Modified residuei480PhosphoserineBy similarity1
Modified residuei621PhosphoserineCombined sources1
Modified residuei622PhosphothreonineCombined sources1
Modified residuei1033PhosphoserineCombined sources1
Modified residuei1034PhosphoserineCombined sources1
Modified residuei1041PhosphoserineCombined sources1
Modified residuei1042PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ64096
MaxQBiQ64096
PRIDEiQ64096

PTM databases

iPTMnetiQ64096
PhosphoSitePlusiQ64096

Expressioni

Tissue specificityi

Expressed at low levels in several hemopoietic cell lines and in thymus and spleen, and at higher levels in other tissues, particularly in brain.1 Publication

Gene expression databases

CleanExiMM_MCF2L

Interactioni

Subunit structurei

Interacts with GTP-bound RAC1 (By similarity). Interacts with CDC42 (PubMed:11889037). Interacts with RHOA (PubMed:12006984). Interacts with CCPG1, which results in specific inhibition of its exchange activity toward RHOA, but does not affect its activity on CDC42 (PubMed:17000758).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC42P60953-24EBI-602123,EBI-287394From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-34544N
IntActiQ64096, 4 interactors
MINTiQ64096

Structurei

Secondary structure

11149
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ64096
SMRiQ64096
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ64096

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 224CRAL-TRIOPROSITE-ProRule annotationAdd BLAST173
Repeati351 – 456SpectrinAdd BLAST106
Domaini632 – 812DHPROSITE-ProRule annotationAdd BLAST181
Domaini841 – 950PHPROSITE-ProRule annotationAdd BLAST110
Domaini1055 – 1116SH3PROSITE-ProRule annotationAdd BLAST62

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili503 – 529Sequence analysisAdd BLAST27

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi624 – 627Poly-Glu4

Domaini

The DH domain is involved in interaction with CCPG1.1 Publication
The CRAL-TRIO domain mediates interaction with various inositol phospholipids, such as phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4-phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P).By similarity

Sequence similaritiesi

Belongs to the MCF2 family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

HOGENOMiHOG000231361
HOVERGENiHBG062385
InParanoidiQ64096

Family and domain databases

CDDicd01227 PH_Dbs, 1 hit
cd00160 RhoGEF, 1 hit
cd00170 SEC14, 1 hit
cd11857 SH3_DBS, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR001251 CRAL-TRIO_dom
IPR036865 CRAL-TRIO_dom_sf
IPR035899 DBL_dom_sf
IPR035534 DBS_PH
IPR035532 DBS_SH3
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR018159 Spectrin/alpha-actinin
IPR002017 Spectrin_repeat
PfamiView protein in Pfam
PF13716 CRAL_TRIO_2, 1 hit
PF00169 PH, 1 hit
PF00621 RhoGEF, 1 hit
PF00435 Spectrin, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SM00516 SEC14, 1 hit
SM00326 SH3, 1 hit
SM00150 SPEC, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF50044 SSF50044, 1 hit
SSF52087 SSF52087, 1 hit
PROSITEiView protein in PROSITE
PS50191 CRAL_TRIO, 1 hit
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS50002 SH3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 17 potential isoforms that are computationally mapped.Show allAlign All

Q64096-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDCWCFIFC KEHVRSNPLS PQHDGASREE ADHQVDVSDG IRLVPDKAEA
60 70 80 90 100
TAATASDEIM HQDIVPLCAA DIQEQLKKRF AYLSGGRGQD GSPVITFPDY
110 120 130 140 150
PAFSEIPDKE FQNVMTYLTS IPSLQDAGIG FILVIDRRQD KWTSVKASVL
160 170 180 190 200
RIAASFPANL QLVLVLRPTG FFQRTLSDIA FKFNRDEFKM KVPVMMLSSV
210 220 230 240 250
PELHGYIDKS QLTEDLGGTL DYCHSRWLCH RTAIESFALM VKQTAQMLQA
260 270 280 290 300
FGTELAETEL PNDVQSTSLV LSAHTEKKAK VKEDLQLALK EGNSILESLR
310 320 330 340 350
EPLAESAAHS VNQDQLDNQA TVQRLLAQLN ETEAAFDEFW AKHQQKLEQC
360 370 380 390 400
LQLRHFEQGF REVKTTLDSM SQKIAAFTDV GNSLAHVQHL LKDLTAFEEK
410 420 430 440 450
SSVAVDKARA LSLEGQQLIE NRHYAVDSIH PKCEELQHLC DHFASEVTRR
460 470 480 490 500
RGLLSKSLEL HSLLETSMKW SDEGIFLLAS QPVDKCQSQD GAEAALQEIE
510 520 530 540 550
KFLETGAENK IQELNEIYKE YECILNQDLL EHVQKVFQKQ ESTEEMFHRR
560 570 580 590 600
QASLKKLAAK QTRPVQPVAP RPEALTKSPS PSPGSWRSSE NSSSEGNALR
610 620 630 640 650
RGPYRRAKSE MSEPRQGRTS STGEEEESLA ILRRHVMNEL LDTERAYVEE
660 670 680 690 700
LLCVLEGYAA EMDNPLMAHL ISTGLQNKKN ILFGNMEEIY HFHNRNIPAG
710 720 730 740 750
LESCIDCPEL VGRCFLERME EFQIYEKYCQ NKPRSESLWR QCSDCPFFQE
760 770 780 790 800
CQKKLDHKLS LDSYLLKPVQ RITKYQLLLK EMLKYSKHCE GAEDLQEALS
810 820 830 840 850
SILGILKAVN DSMHLIAITG YDGNLGDLGK LLMQGSFSVW TDHKKGHTKV
860 870 880 890 900
KELARFKPMQ RHLFLHEKAV LFCKKREENG EGYEKAPSYS YKQSLNMTAV
910 920 930 940 950
GITENVKGDT KKFEIWYNAR EEVYIIQAPT PEIKAAWVNE IRKVLTSQLQ
960 970 980 990 1000
ACREASQHRA LEQSHSLPLP TPSSTSPTKG NTRNVKKLED RKTDPLSLEG
1010 1020 1030 1040 1050
YVSSSLPKPP EKGKGWSKTS HSLEAPEEDG GWSSAEELIN SSDAEEDGGV
1060 1070 1080 1090 1100
GPKKLVPGKY TVVMDDEKGG PDTLAMRSGD MVEVVEEGAE GLWYVRDLTS
1110 1120 1130 1140
SKEGWVPASS LSTLLGKSSS AQCLSSSGKI HCARQLCPEP AEILSPEPV
Length:1,149
Mass (Da):129,113
Last modified:July 27, 2011 - v2
Checksum:i0A325FB42E49BA6D
GO

Computationally mapped potential isoform sequencesi

There are 17 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YUF4D3YUF4_MOUSE
Guanine nucleotide exchange factor ...
Mcf2l
1,046Annotation score:
E9PXE2E9PXE2_MOUSE
Guanine nucleotide exchange factor ...
Mcf2l
1,175Annotation score:
E9PY13E9PY13_MOUSE
Guanine nucleotide exchange factor ...
Mcf2l
1,097Annotation score:
E9PY12E9PY12_MOUSE
Guanine nucleotide exchange factor ...
Mcf2l
1,101Annotation score:
E9Q863E9Q863_MOUSE
Guanine nucleotide exchange factor ...
Mcf2l
1,067Annotation score:
E9PXE1E9PXE1_MOUSE
Guanine nucleotide exchange factor ...
Mcf2l
1,125Annotation score:
G3UX72G3UX72_MOUSE
Guanine nucleotide exchange factor ...
Mcf2l
1,118Annotation score:
D3YUF3D3YUF3_MOUSE
Guanine nucleotide exchange factor ...
Mcf2l
866Annotation score:
G3UYM8G3UYM8_MOUSE
Guanine nucleotide exchange factor ...
Mcf2l
757Annotation score:
E9QPM7E9QPM7_MOUSE
Guanine nucleotide exchange factor ...
Mcf2l
1,149Annotation score:
There are more potential isoformsShow all

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti495 – 496AL → SF in AAB33461 (PubMed:7862449).Curated2
Sequence conflicti701L → V in AAB33461 (PubMed:7862449).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S76838 mRNA Translation: AAB33461.2
AC127308 Genomic DNA No translation available.
AC134581 Genomic DNA No translation available.
UniGeneiMm.334413

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S76838 mRNA Translation: AAB33461.2
AC127308 Genomic DNA No translation available.
AC134581 Genomic DNA No translation available.
UniGeneiMm.334413

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KZ7X-ray2.40A/C623-967[»]
1KZGX-ray2.60A/C623-967[»]
1LB1X-ray2.81A/C/E/G623-967[»]
1RJ2X-ray3.00A/D/G/J623-968[»]
ProteinModelPortaliQ64096
SMRiQ64096
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-34544N
IntActiQ64096, 4 interactors
MINTiQ64096

PTM databases

iPTMnetiQ64096
PhosphoSitePlusiQ64096

Proteomic databases

EPDiQ64096
MaxQBiQ64096
PRIDEiQ64096

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:103263 Mcf2l

Phylogenomic databases

HOGENOMiHOG000231361
HOVERGENiHBG062385
InParanoidiQ64096

Miscellaneous databases

ChiTaRSiMcf2l mouse
EvolutionaryTraceiQ64096
PROiPR:Q64096
SOURCEiSearch...

Gene expression databases

CleanExiMM_MCF2L

Family and domain databases

CDDicd01227 PH_Dbs, 1 hit
cd00160 RhoGEF, 1 hit
cd00170 SEC14, 1 hit
cd11857 SH3_DBS, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR001251 CRAL-TRIO_dom
IPR036865 CRAL-TRIO_dom_sf
IPR035899 DBL_dom_sf
IPR035534 DBS_PH
IPR035532 DBS_SH3
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR018159 Spectrin/alpha-actinin
IPR002017 Spectrin_repeat
PfamiView protein in Pfam
PF13716 CRAL_TRIO_2, 1 hit
PF00169 PH, 1 hit
PF00621 RhoGEF, 1 hit
PF00435 Spectrin, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SM00516 SEC14, 1 hit
SM00326 SH3, 1 hit
SM00150 SPEC, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF50044 SSF50044, 1 hit
SSF52087 SSF52087, 1 hit
PROSITEiView protein in PROSITE
PS50191 CRAL_TRIO, 1 hit
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS50002 SH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMCF2L_MOUSE
AccessioniPrimary (citable) accession number: Q64096
Secondary accession number(s): E9PV70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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