UniProtKB - Q64060 (DDX4_RAT)
Protein
Probable ATP-dependent RNA helicase DDX4
Gene
Ddx4
Organism
Rattus norvegicus (Rat)
Status
Functioni
ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs.By similarity
Catalytic activityi
- EC:3.6.4.13By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 317 – 324 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATPase activity Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- RNA binding Source: GO_Central
- RNA helicase activity Source: GO_Central
GO - Biological processi
- cell differentiation Source: GO_Central
- DNA methylation involved in gamete generation Source: UniProtKB
- flagellated sperm motility Source: RGD
- gamete generation Source: GO_Central
- gene silencing by RNA Source: UniProtKB-KW
- male meiosis I Source: UniProtKB
- male meiotic nuclear division Source: UniProtKB
- multicellular organism development Source: UniProtKB-KW
- negative regulation of transposition Source: UniProtKB
- piRNA biosynthetic process Source: UniProtKB
- piRNA metabolic process Source: UniProtKB
- regulation of protein localization Source: RGD
- spermatogenesis Source: RGD
Keywordsi
Molecular function | Developmental protein, Helicase, Hydrolase |
Biological process | Differentiation, Meiosis, RNA-mediated gene silencing, Spermatogenesis |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Probable ATP-dependent RNA helicase DDX4 (EC:3.6.4.13By similarity)Alternative name(s): DEAD box protein 4 Vasa homolog Short name: rVLG |
Gene namesi | Name:Ddx4 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1308793, Ddx4 |
Subcellular locationi
Other locations
- Cytoplasm By similarity
- perinuclear region By similarity
Note: Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.By similarity
Nucleus
- nucleus Source: RGD
Other locations
- chromatoid body Source: RGD
- cytoplasm Source: UniProtKB
- P granule Source: GO_Central
- perinuclear region of cytoplasm Source: RGD
- pi-body Source: UniProtKB
- piP-body Source: UniProtKB
- ribonucleoprotein complex Source: RGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054981 | 1 – 713 | Probable ATP-dependent RNA helicase DDX4Add BLAST | 713 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 207 | PhosphoserineCombined sources | 1 | |
Modified residuei | 211 | PhosphoserineBy similarity | 1 | |
Modified residuei | 711 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q64060 |
PaxDbi | Q64060 |
PRIDEi | Q64060 |
PTM databases
iPTMneti | Q64060 |
PhosphoSitePlusi | Q64060 |
Expressioni
Tissue specificityi
Testis.
Interactioni
Subunit structurei
Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4.
Interacts with RANBP9.
Interacts with RANBP10.
Interacts with PIWIL2 and MAEL.
Interacts with ARNTL/BMAL1 and CLOCK.
Interacts with Tex19.1 and, probably, Tex19.2.
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000013035 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 304 – 487 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 184 | |
Domaini | 515 – 660 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 146 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 213 – 232 | Interaction with RANBP9By similarityAdd BLAST | 20 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 273 – 301 | Q motifAdd BLAST | 29 | |
Motifi | 431 – 434 | DEAD boxBy similarity | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 58 – 219 | Gly-richAdd BLAST | 162 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0335, Eukaryota |
InParanoidi | Q64060 |
OrthoDBi | 595675at2759 |
PhylomeDBi | Q64060 |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q64060-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGDEDWEAEI LKPHVSSYVP VFEKDKYSSG ANGDTFNRTS ASSSEMEDGP
60 70 80 90 100
SGRDHFMRSG FSSGRNLGNR DIGESSKRET TSTTGGFGRG KGFGNRGFLN
110 120 130 140 150
NKFEEGDSSG FWKESTNDCE DTQTRSRGFS KRGGYPDGND SEASGPFRRG
160 170 180 190 200
GRDSEYDQDQ GSQRGGGLFG SRKPAASDSG SGDTFQSRSG NARGAYKGLN
210 220 230 240 250
EEVVTGSGKN SWKSEAEGGE SSDIQGPKVT YIPPPPPEDE DSIFAHYQTG
260 270 280 290 300
INFDKYDTIL VEVSGHDAPP AILTFEEANL CQTLNNNIAK AGYTKLTPVQ
310 320 330 340 350
KYSIPIVLAG RDLMACAQTG SGKTAAFLLP ILAHMMRDGI TASRFKELQE
360 370 380 390 400
PECIIVAPTR ELINQIYLEA RKFSFGTCVR AVVIYGGTQF GHSIRQIVQG
410 420 430 440 450
CNILCATPGR LMDIIGKEKI GLKQVKYLVL DEADRMLDMG FGPEMKKLIS
460 470 480 490 500
CPGMPSKEQR QTLLFSATFP EEIQRLAGEF LKSNYLFVAV GQVGGACRDV
510 520 530 540 550
QQSILQVGPV FKKRKLVEIL RNIGDERPMV FVETKKKADF IATFLCQEKI
560 570 580 590 600
STTSIHGDRE QREREQALGD FRCGKCPVLV ATSVAARGLD IENVQHVINF
610 620 630 640 650
NLPSTIDEYV HRIGRTGRCG NTGRAISFFD TESDNHLAQP LVKVLSDAQQ
660 670 680 690 700
DVPAWLEEIA FSSYAPPSFS NSTRGAVFAS FDTRKNFQGK NTLNTAGISS
710
AQAPNPVDDE SWD
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF1LQD1 | F1LQD1_RAT | RNA helicase | Ddx4 | 708 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S75275 mRNA Translation: AAB33364.1 |
PIRi | JC2534 |
RefSeqi | NP_001071115.1, NM_001077647.1 |
Genome annotation databases
GeneIDi | 310090 |
KEGGi | rno:310090 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S75275 mRNA Translation: AAB33364.1 |
PIRi | JC2534 |
RefSeqi | NP_001071115.1, NM_001077647.1 |
3D structure databases
SMRi | Q64060 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000013035 |
PTM databases
iPTMneti | Q64060 |
PhosphoSitePlusi | Q64060 |
Proteomic databases
jPOSTi | Q64060 |
PaxDbi | Q64060 |
PRIDEi | Q64060 |
Genome annotation databases
GeneIDi | 310090 |
KEGGi | rno:310090 |
Organism-specific databases
CTDi | 54514 |
RGDi | 1308793, Ddx4 |
Phylogenomic databases
eggNOGi | KOG0335, Eukaryota |
InParanoidi | Q64060 |
OrthoDBi | 595675at2759 |
PhylomeDBi | Q64060 |
Miscellaneous databases
PROi | PR:Q64060 |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DDX4_RAT | |
Accessioni | Q64060Primary (citable) accession number: Q64060 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families