UniProtKB - Q63YW6 (Q63YW6_BURPS)
Protein
Beta sliding clamp
Gene
dnaN
Organism
Burkholderia pseudomallei (strain K96243)
Status
Functioni
Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.UniRule annotation
GO - Molecular functioni
- 3'-5' exonuclease activity Source: InterPro
- DNA binding Source: UniProtKB-UniRule
- DNA-directed DNA polymerase activity Source: UniProtKB-UniRule
GO - Biological processi
- DNA replication Source: UniProtKB-UniRule
Keywordsi
Molecular function | DNA-bindingARBA annotation, DNA-directed DNA polymeraseUniRule annotationARBA annotation, Nucleotidyltransferase, Transferase |
Biological process | DNA replicationUniRule annotationARBA annotation |
Enzyme and pathway databases
BioCyci | BPSE272560:G1G3Q-74-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Beta sliding clampUniRule annotationARBA annotation |
Gene namesi | Name:dnaNImported Ordered Locus Names:BPSL0074Imported |
Organismi | Burkholderia pseudomallei (strain K96243)Imported |
Taxonomic identifieri | 272560 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotationARBA annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
- DNA polymerase III complex Source: InterPro
Keywords - Cellular componenti
CytoplasmUniRule annotationARBA annotationInteractioni
Subunit structurei
Forms a ring-shaped head-to-tail homodimer around DNA.
UniRule annotationProtein-protein interaction databases
STRINGi | 272560.BPSL0074 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 119 | DNA_pol3_betaInterPro annotationAdd BLAST | 114 | |
Domaini | 131 – 244 | DNA_pol3_beta_2InterPro annotationAdd BLAST | 114 | |
Domaini | 247 – 366 | DNA_pol3_beta_3InterPro annotationAdd BLAST | 120 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0592, Bacteria |
OMAi | YLIMPVR |
Family and domain databases
CDDi | cd00140, beta_clamp, 1 hit |
InterProi | View protein in InterPro IPR001001, DNA_polIII_beta IPR022635, DNA_polIII_beta_C IPR022637, DNA_polIII_beta_cen IPR022634, DNA_polIII_beta_N |
PANTHERi | PTHR30478, PTHR30478, 1 hit |
Pfami | View protein in Pfam PF00712, DNA_pol3_beta, 1 hit PF02767, DNA_pol3_beta_2, 1 hit PF02768, DNA_pol3_beta_3, 1 hit |
PIRSFi | PIRSF000804, DNA_pol_III_b, 1 hit |
SMARTi | View protein in SMART SM00480, POL3Bc, 1 hit |
TIGRFAMsi | TIGR00663, dnan, 1 hit |
i Sequence
Sequence statusi: Complete.
Q63YW6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQLVKTERDT LLRPLQTVSG IVERRHTLPI LANLLITKNG PDVSFLSTDL
60 70 80 90 100
ELQITTRADF GVGGDQVATT VAARKLLDIL RAMPDGQVTL SLADKRLTVQ
110 120 130 140 150
SGKSRFALQT LAADEFPTVA QSKDFGASLA VPQKAFRQLL GMVYFAMAQQ
160 170 180 190 200
DIRYYLNGML LVVDGDRLMA VATDGHRLAF SSMKIEGSFG RQEVIVPRKT
210 220 230 240 250
ILELQRLLED IDDTVKIDIA QTQAKFTFGQ VELVSKLVEG KFPDFQRVIP
260 270 280 290 300
KAHKNTFEIG REELQRSLQR AAILTSDKFK GVRCIIAPGQ LKIMSTNADQ
310 320 330 340 350
EEAQEELEIA YQGDTVDIGF NVTYLLDVLA NLKVDTVQVS LGDASSSALI
360
TVPENDEFKY VVMPMRI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX571965 Genomic DNA Translation: CAH34057.1 |
RefSeqi | WP_004196275.1, NZ_CP009538.1 YP_106699.1, NC_006350.1 |
Genome annotation databases
EnsemblBacteriai | CAH34057; CAH34057; BPSL0074 |
GeneIDi | 56593630 |
KEGGi | bps:BPSL0074 |
PATRICi | fig|272560.51.peg.1661 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX571965 Genomic DNA Translation: CAH34057.1 |
RefSeqi | WP_004196275.1, NZ_CP009538.1 YP_106699.1, NC_006350.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 272560.BPSL0074 |
Genome annotation databases
EnsemblBacteriai | CAH34057; CAH34057; BPSL0074 |
GeneIDi | 56593630 |
KEGGi | bps:BPSL0074 |
PATRICi | fig|272560.51.peg.1661 |
Phylogenomic databases
eggNOGi | COG0592, Bacteria |
OMAi | YLIMPVR |
Enzyme and pathway databases
BioCyci | BPSE272560:G1G3Q-74-MONOMER |
Family and domain databases
CDDi | cd00140, beta_clamp, 1 hit |
InterProi | View protein in InterPro IPR001001, DNA_polIII_beta IPR022635, DNA_polIII_beta_C IPR022637, DNA_polIII_beta_cen IPR022634, DNA_polIII_beta_N |
PANTHERi | PTHR30478, PTHR30478, 1 hit |
Pfami | View protein in Pfam PF00712, DNA_pol3_beta, 1 hit PF02767, DNA_pol3_beta_2, 1 hit PF02768, DNA_pol3_beta_3, 1 hit |
PIRSFi | PIRSF000804, DNA_pol_III_b, 1 hit |
SMARTi | View protein in SMART SM00480, POL3Bc, 1 hit |
TIGRFAMsi | TIGR00663, dnan, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | Q63YW6_BURPS | |
Accessioni | Q63YW6Primary (citable) accession number: Q63YW6 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | October 25, 2004 |
Last sequence update: | October 25, 2004 | |
Last modified: | April 7, 2021 | |
This is version 102 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |