UniProtKB - Q63YJ9 (GATA_BURPS)
Protein
Glutamyl-tRNA(Gln) amidotransferase subunit A
Gene
gatA
Organism
Burkholderia pseudomallei (strain K96243)
Status
Functioni
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation
Catalytic activityi
- ATP + H2O + L-glutamine + L-glutamyl-tRNAGln = ADP + H+ + L-glutamate + L-glutaminyl-tRNAGln + phosphateUniRule annotationEC:6.3.5.7UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 75 | Charge relay systemUniRule annotation | 1 | |
Active sitei | 150 | Charge relay systemUniRule annotation | 1 | |
Active sitei | 174 | Acyl-ester intermediateUniRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Source: UniProtKB-UniRule
- hydrolase activity Source: InterPro
GO - Biological processi
- translation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BPSE272560:G1G3Q-197-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)Short name: Glu-ADT subunit AUniRule annotation |
Gene namesi | Name:gatAUniRule annotation Ordered Locus Names:BPSL0188 |
Organismi | Burkholderia pseudomallei (strain K96243) |
Taxonomic identifieri | 272560 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000241082 | 1 – 496 | Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST | 496 |
Interactioni
Subunit structurei
Heterotrimer of A, B and C subunits.
UniRule annotationProtein-protein interaction databases
STRINGi | 272560.BPSL0188 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0154, Bacteria |
OMAi | MYLSDIF |
Family and domain databases
Gene3Di | 3.90.1300.10, 1 hit |
HAMAPi | MF_00120, GatA, 1 hit |
InterProi | View protein in InterPro IPR000120, Amidase IPR020556, Amidase_CS IPR023631, Amidase_dom IPR036928, AS_sf IPR004412, GatA |
PANTHERi | PTHR11895, PTHR11895, 1 hit |
Pfami | View protein in Pfam PF01425, Amidase, 1 hit |
SUPFAMi | SSF75304, SSF75304, 1 hit |
TIGRFAMsi | TIGR00132, gatA, 1 hit |
PROSITEi | View protein in PROSITE PS00571, AMIDASES, 1 hit |
i Sequence
Sequence statusi: Complete.
Q63YJ9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHAKSLTELR AALDAKECSA VELAQHYLKR IDAARDLNAF VHVDAELTLA
60 70 80 90 100
QAKAADAALA NGEAGPLAGL PIAHKDVFVT RGWRSTAGSK MLANYASPFD
110 120 130 140 150
ATVVARLSAA GMVTLGKTNM DEFAMGSSNE NSAFGPVKNP WDTSAVPGGS
160 170 180 190 200
SGGSSAAVAA RLAPAATGTD TGGSIRQPAS FAGVTGIKPT YGRVSRYGMI
210 220 230 240 250
AFASSLDQGG PMARSAADCA LLLNAMAGFD ERDSTSLERA DEDYTRHLGK
260 270 280 290 300
AWAAGGDAGK PLAGLRIGLP AEYFGAGLAD DVRAAIDAAL KTYEALGATL
310 320 330 340 350
VPVSLPKTEL SIPVYYVIAP AEASSNLSRF DGVRYGHRAA EYRDLLDMYK
360 370 380 390 400
KSRAEGFGPE VKRRILVGTY VLSHGYYDAY YLQAQKIRRI IAQDFQEAFK
410 420 430 440 450
SCDVIMGPAS PTVAWDIGAK GDDPVQMYLA DIYTLSVSLA GLPGMSVPCG
460 470 480 490
FGAGANAKRP VGLQIIGNYF DEARMLQVAD AFQRATDWHV QEPAGV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX571965 Genomic DNA Translation: CAH34175.1 |
RefSeqi | WP_004525859.1, NZ_CP009538.1 YP_106816.1, NC_006350.1 |
Genome annotation databases
EnsemblBacteriai | CAH34175; CAH34175; BPSL0188 |
GeneIDi | 56527572 |
KEGGi | bps:BPSL0188 |
PATRICi | fig|272560.51.peg.1531 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX571965 Genomic DNA Translation: CAH34175.1 |
RefSeqi | WP_004525859.1, NZ_CP009538.1 YP_106816.1, NC_006350.1 |
3D structure databases
SMRi | Q63YJ9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 272560.BPSL0188 |
Genome annotation databases
EnsemblBacteriai | CAH34175; CAH34175; BPSL0188 |
GeneIDi | 56527572 |
KEGGi | bps:BPSL0188 |
PATRICi | fig|272560.51.peg.1531 |
Phylogenomic databases
eggNOGi | COG0154, Bacteria |
OMAi | MYLSDIF |
Enzyme and pathway databases
BioCyci | BPSE272560:G1G3Q-197-MONOMER |
Family and domain databases
Gene3Di | 3.90.1300.10, 1 hit |
HAMAPi | MF_00120, GatA, 1 hit |
InterProi | View protein in InterPro IPR000120, Amidase IPR020556, Amidase_CS IPR023631, Amidase_dom IPR036928, AS_sf IPR004412, GatA |
PANTHERi | PTHR11895, PTHR11895, 1 hit |
Pfami | View protein in Pfam PF01425, Amidase, 1 hit |
SUPFAMi | SSF75304, SSF75304, 1 hit |
TIGRFAMsi | TIGR00132, gatA, 1 hit |
PROSITEi | View protein in PROSITE PS00571, AMIDASES, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GATA_BURPS | |
Accessioni | Q63YJ9Primary (citable) accession number: Q63YJ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 27, 2006 |
Last sequence update: | October 25, 2004 | |
Last modified: | April 7, 2021 | |
This is version 82 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families