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Protein

AP-1 complex-associated regulatory protein

Gene

AP1AR

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Necessary for adaptor protein complex 1 (AP-1)-dependent transport between the trans-Golgi network and endosomes. Regulates the membrane association of AP1G1/gamma1-adaptin, one of the subunits of the AP-1 adaptor complex. The direct interaction with AP1G1/gamma1-adaptin attenuates the release of the AP-1 complex from membranes. Regulates endosomal membrane traffic via association with AP-1 and KIF5B thus linking kinesin-based plus-end-directed microtubular transport to AP-1-dependent membrane traffic. May act as effector of AP-1 in calcium-induced endo-lysosome secretion. Inhibits Arp2/3 complex function; negatively regulates cell spreading, size and motility via intracellular sequestration of the Arp2/3 complex.4 Publications

GO - Molecular functioni

  • AP-1 adaptor complex binding Source: UniProtKB
  • kinesin binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex-associated regulatory protein
Alternative name(s):
2c18
Adaptor-related protein complex 1-associated regulatory protein
Gamma-1-adaptin brefeldin A resistance protein
Short name:
GBAR
Short name:
Gamma-BAR
Gamma-A1-adaptin and kinesin interactor
Short name:
Gadkin
Gene namesi
Name:AP1AR
Synonyms:C4orf16
ORF Names:PRO0971
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000138660.11
HGNCiHGNC:28808 AP1AR
MIMi610851 gene
neXtProtiNX_Q63HQ0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi4 – 5CC → SS: Loss of association with membranes, no effect on interaction with AP1G1; when associated with 9-S. 1 Publication2
Mutagenesisi9C → S: Loss of association with membranes, no effect on interaction with AP1G1; when associated with 4-S-S-5. 1 Publication1
Mutagenesisi210W → A: Loss of association with the Arp2/3 complex and endosomal colocalization. Abolishes interaction with KLC2, no effect on interaction with AP1G1. 2 Publications1
Mutagenesisi260W → L: Decreases interaction with AP1G1; when associated with L-264. 1 Publication1
Mutagenesisi264F → L: Decreases interaction with AP1G1; when associated with L-260. 1 Publication1

Organism-specific databases

DisGeNETi55435
OpenTargetsiENSG00000138660
PharmGKBiPA165663153

Polymorphism and mutation databases

BioMutaiAP1AR
DMDMi73917693

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000894321 – 302AP-1 complex-associated regulatory proteinAdd BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29PhosphoserineCombined sources1
Modified residuei226PhosphoserineCombined sources1
Modified residuei228PhosphothreonineCombined sources1

Post-translational modificationi

Palmitoylated.1 Publication

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiQ63HQ0
MaxQBiQ63HQ0
PaxDbiQ63HQ0
PeptideAtlasiQ63HQ0
PRIDEiQ63HQ0
ProteomicsDBi65888
65889 [Q63HQ0-2]

PTM databases

iPTMnetiQ63HQ0
PhosphoSitePlusiQ63HQ0
SwissPalmiQ63HQ0

Expressioni

Gene expression databases

BgeeiENSG00000138660 Expressed in 210 organ(s), highest expression level in caudate nucleus
CleanExiHS_C4orf16
ExpressionAtlasiQ63HQ0 baseline and differential
GenevisibleiQ63HQ0 HS

Organism-specific databases

HPAiHPA035863
HPA058115

Interactioni

Subunit structurei

Interacts (via coiled-coil domain) with AP1G1 (via GAE domain). Interacts with KIF5B. Associates with the Arp2/3 complex.3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi120667, 4 interactors
CORUMiQ63HQ0
ELMiQ63HQ0
IntActiQ63HQ0, 4 interactors
STRINGi9606.ENSP00000274000

Structurei

3D structure databases

ProteinModelPortaliQ63HQ0
SMRiQ63HQ0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni78 – 138Interaction with AP1G1Add BLAST61
Regioni199 – 215Sufficient for association with the Arp2/3 complexAdd BLAST17

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili80 – 138Sequence analysisAdd BLAST59

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi24 – 28Poly-Gly5

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IK27 Eukaryota
ENOG4111HXC LUCA
GeneTreeiENSGT00390000002219
HOGENOMiHOG000237335
HOVERGENiHBG059469
InParanoidiQ63HQ0
OMAiSNNGEYQ
OrthoDBiEOG091G127J
PhylomeDBiQ63HQ0
TreeFamiTF335676

Family and domain databases

InterProiView protein in InterPro
IPR031483 AP1AR
PANTHERiPTHR34529 PTHR34529, 1 hit
PfamiView protein in Pfam
PF15745 AP1AR, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q63HQ0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGNCCWTQCF GLLRKEAGRL QRVGGGGGSK YFRTCSRGEH LTIEFENLVE
60 70 80 90 100
SDEGESPGSS HRPLTEEEIV DLRERHYDSI AEKQKDLDKK IQKELALQEE
110 120 130 140 150
KLRLEEEALY AAQREAARAA KQRKLLEQER QRIVQQYHPS NNGEYQSSGP
160 170 180 190 200
EDDFESCLRN MKSQYEVFRS SRLSSDATVL TPNTESSCDL MTKTKSTSGN
210 220 230 240 250
DDSTSLDLEW EDEEGMNRML PMRERSKTEE DILRAALKYS NKKTGSNPTS
260 270 280 290 300
ASDDSNGLEW ENDFVSAEMD DNGNSEYSGF VNPVLELSDS GIRHSDTDQQ

TR
Length:302
Mass (Da):34,280
Last modified:October 25, 2004 - v1
Checksum:i0827B5EC95DC133A
GO
Isoform 2 (identifier: Q63HQ0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     94-126: Missing.

Show »
Length:269
Mass (Da):30,459
Checksum:i63DCDB9D74E94F2B
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0Y8Y4H0Y8Y4_HUMAN
AP-1 complex-associated regulatory ...
AP1AR
76Annotation score:

Sequence cautioni

The sequence AAF71037 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89K → E in CAB66563 (PubMed:11230166).Curated1
Sequence conflicti217N → D in BAG37704 (PubMed:14702039).Curated1
Sequence conflicti243K → E in AAH09485 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050769297T → I. Corresponds to variant dbSNP:rs34900583Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01533994 – 126Missing in isoform 2. 1 PublicationAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136628 mRNA Translation: CAB66563.1
BX647702 mRNA Translation: CAH56145.1
AK315299 mRNA Translation: BAG37704.1
AC109347 Genomic DNA Translation: AAY40962.1
BC009485 mRNA Translation: AAH09485.1
AF116612 mRNA Translation: AAF71037.1 Different initiation.
CCDSiCCDS3696.1 [Q63HQ0-1]
CCDS47125.1 [Q63HQ0-2]
RefSeqiNP_001121898.1, NM_001128426.2 [Q63HQ0-2]
NP_061039.3, NM_018569.5 [Q63HQ0-1]
UniGeneiHs.435991
Hs.550015
Hs.606210

Genome annotation databases

EnsembliENST00000274000; ENSP00000274000; ENSG00000138660 [Q63HQ0-1]
ENST00000309703; ENSP00000309023; ENSG00000138660 [Q63HQ0-2]
GeneIDi55435
KEGGihsa:55435
UCSCiuc003iaj.6 human [Q63HQ0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136628 mRNA Translation: CAB66563.1
BX647702 mRNA Translation: CAH56145.1
AK315299 mRNA Translation: BAG37704.1
AC109347 Genomic DNA Translation: AAY40962.1
BC009485 mRNA Translation: AAH09485.1
AF116612 mRNA Translation: AAF71037.1 Different initiation.
CCDSiCCDS3696.1 [Q63HQ0-1]
CCDS47125.1 [Q63HQ0-2]
RefSeqiNP_001121898.1, NM_001128426.2 [Q63HQ0-2]
NP_061039.3, NM_018569.5 [Q63HQ0-1]
UniGeneiHs.435991
Hs.550015
Hs.606210

3D structure databases

ProteinModelPortaliQ63HQ0
SMRiQ63HQ0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120667, 4 interactors
CORUMiQ63HQ0
ELMiQ63HQ0
IntActiQ63HQ0, 4 interactors
STRINGi9606.ENSP00000274000

PTM databases

iPTMnetiQ63HQ0
PhosphoSitePlusiQ63HQ0
SwissPalmiQ63HQ0

Polymorphism and mutation databases

BioMutaiAP1AR
DMDMi73917693

Proteomic databases

EPDiQ63HQ0
MaxQBiQ63HQ0
PaxDbiQ63HQ0
PeptideAtlasiQ63HQ0
PRIDEiQ63HQ0
ProteomicsDBi65888
65889 [Q63HQ0-2]

Protocols and materials databases

DNASUi55435
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000274000; ENSP00000274000; ENSG00000138660 [Q63HQ0-1]
ENST00000309703; ENSP00000309023; ENSG00000138660 [Q63HQ0-2]
GeneIDi55435
KEGGihsa:55435
UCSCiuc003iaj.6 human [Q63HQ0-1]

Organism-specific databases

CTDi55435
DisGeNETi55435
EuPathDBiHostDB:ENSG00000138660.11
GeneCardsiAP1AR
HGNCiHGNC:28808 AP1AR
HPAiHPA035863
HPA058115
MIMi610851 gene
neXtProtiNX_Q63HQ0
OpenTargetsiENSG00000138660
PharmGKBiPA165663153
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IK27 Eukaryota
ENOG4111HXC LUCA
GeneTreeiENSGT00390000002219
HOGENOMiHOG000237335
HOVERGENiHBG059469
InParanoidiQ63HQ0
OMAiSNNGEYQ
OrthoDBiEOG091G127J
PhylomeDBiQ63HQ0
TreeFamiTF335676

Miscellaneous databases

ChiTaRSiAP1AR human
GeneWikiiAP1AR
GenomeRNAii55435
PROiPR:Q63HQ0
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000138660 Expressed in 210 organ(s), highest expression level in caudate nucleus
CleanExiHS_C4orf16
ExpressionAtlasiQ63HQ0 baseline and differential
GenevisibleiQ63HQ0 HS

Family and domain databases

InterProiView protein in InterPro
IPR031483 AP1AR
PANTHERiPTHR34529 PTHR34529, 1 hit
PfamiView protein in Pfam
PF15745 AP1AR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAP1AR_HUMAN
AccessioniPrimary (citable) accession number: Q63HQ0
Secondary accession number(s): B2RCV7
, Q96GG6, Q9H0V0, Q9P1L4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 25, 2004
Last modified: November 7, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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