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Protein

Kynurenine formamidase

Gene

AFMID

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.UniRule annotation

Catalytic activityi

N-formyl-L-kynurenine + H2O = formate + L-kynurenine.UniRule annotation

Pathwayi: L-tryptophan degradation via kynurenine pathway

This protein is involved in step 2 of the subpathway that synthesizes L-kynurenine from L-tryptophan.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Indoleamine 2,3-dioxygenase 1 (IDO1), Indoleamine 2,3-dioxygenase 2 (IDO2), Tryptophan 2,3-dioxygenase (TDO2)
  2. Kynurenine formamidase (AFMID)
This subpathway is part of the pathway L-tryptophan degradation via kynurenine pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-kynurenine from L-tryptophan, the pathway L-tryptophan degradation via kynurenine pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei164NucleophileUniRule annotation1
Active sitei247UniRule annotation1
Active sitei279UniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • 'de novo' NAD biosynthetic process from tryptophan Source: UniProtKB-UniRule
  • tryptophan catabolic process to kynurenine Source: GO_Central

Keywordsi

Molecular functionHydrolase
Biological processTryptophan catabolism

Enzyme and pathway databases

ReactomeiR-HSA-71240 Tryptophan catabolism
UniPathwayi
UPA00333;UER00454

Protein family/group databases

ESTHERihuman-AFMID Kynurenine-formamidase
MEROPSiS09.977

Names & Taxonomyi

Protein namesi
Recommended name:
Kynurenine formamidaseUniRule annotation (EC:3.5.1.9UniRule annotation)
Short name:
KFAUniRule annotation
Short name:
KFaseUniRule annotation
Alternative name(s):
ArylformamidaseUniRule annotation
N-formylkynurenine formamidaseUniRule annotation
Short name:
FKFUniRule annotation
Gene namesi
Name:AFMIDUniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000183077.15
HGNCiHGNC:20910 AFMID
neXtProtiNX_Q63HM1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000183077
PharmGKBiPA134958475

Polymorphism and mutation databases

BioMutaiAFMID
DMDMi259016175

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002483081 – 303Kynurenine formamidaseAdd BLAST303

Proteomic databases

EPDiQ63HM1
MaxQBiQ63HM1
PaxDbiQ63HM1
PeptideAtlasiQ63HM1
PRIDEiQ63HM1
ProteomicsDBi65879
65880 [Q63HM1-2]

PTM databases

iPTMnetiQ63HM1
PhosphoSitePlusiQ63HM1

Expressioni

Gene expression databases

BgeeiENSG00000183077 Expressed in 113 organ(s), highest expression level in right lobe of liver
CleanExiHS_AFMID
ExpressionAtlasiQ63HM1 baseline and differential

Organism-specific databases

HPAiHPA023861
HPA026536

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

BioGridi125914, 6 interactors
IntActiQ63HM1, 2 interactors
STRINGi9606.ENSP00000328938

Structurei

3D structure databases

ProteinModelPortaliQ63HM1
SMRiQ63HM1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi95 – 99HGGXW5

Domaini

The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.UniRule annotation

Sequence similaritiesi

Belongs to the kynurenine formamidase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG4627 Eukaryota
COG0657 LUCA
GeneTreeiENSGT00390000011093
HOGENOMiHOG000260457
HOVERGENiHBG100436
InParanoidiQ63HM1
KOiK01432
OMAiKEESGFM
OrthoDBiEOG091G0KT8
PhylomeDBiQ63HM1
TreeFamiTF315112

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
HAMAPiMF_03014 KFase, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR013094 AB_hydrolase_3
IPR027519 KFase_ver/fungi-typ
PfamiView protein in Pfam
PF07859 Abhydrolase_3, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q63HM1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MMDVSGVGFP SKVPWKKMSA EELENQYCPS RWVVRLGAEE ALRTYSQIGI
60 70 80 90 100
EATTRARATR KSLLHVPYGD GEGEKVDIYF PDESSEALPF FLFFHGGYWQ
110 120 130 140 150
SGSKDESAFM VHPLTAQGVA VVIVAYGIAP KGTLDHMVDQ VTRSVAFVQK
160 170 180 190 200
RYPSNKGIYL CGHSAGAHLA AMMLLADWTK HGVTPNLRGF FLVSGVFDLE
210 220 230 240 250
PIVYTSQNVA LQLTLEDAQR NSPQLKVAQA QPVDPTCRVL VVVGQFDSPE
260 270 280 290 300
FHRQSWEFYQ TLCQGEWKAS FEELHDVDHF EIVENLTQKD NVLTQIILKT

IFQ
Length:303
Mass (Da):33,992
Last modified:September 22, 2009 - v2
Checksum:i6E329218366C027D
GO
Isoform 2 (identifier: Q63HM1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     260-260: Q → QVLPVQ

Note: No experimental confirmation available.
Show »
Length:308
Mass (Da):34,528
Checksum:i06640990D1E1B57C
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7EMI4K7EMI4_HUMAN
Kynurenine formamidase
AFMID
206Annotation score:
K7EMM5K7EMM5_HUMAN
Kynurenine formamidase
AFMID
187Annotation score:
K7EK09K7EK09_HUMAN
Kynurenine formamidase
AFMID
111Annotation score:
W4VSQ7W4VSQ7_HUMAN
Arylformamidase, isoform CRA_b
AFMID hCG_29397
57Annotation score:
K7ELV9K7ELV9_HUMAN
Kynurenine formamidase
AFMID
37Annotation score:
K7EQK5K7EQK5_HUMAN
Kynurenine formamidase
AFMID
60Annotation score:
K7EPF8K7EPF8_HUMAN
Kynurenine formamidase
AFMID
84Annotation score:
K7EIX3K7EIX3_HUMAN
Kynurenine formamidase
AFMID
93Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti222S → N in CAH56149 (PubMed:17974005).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038002260Q → QVLPVQ in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX648442 mRNA Translation: CAH56149.1
BC132824 mRNA Translation: AAI32825.1
CCDSiCCDS32750.2 [Q63HM1-1]
CCDS45801.1 [Q63HM1-2]
RefSeqiNP_001010982.2, NM_001010982.4 [Q63HM1-1]
NP_001138998.1, NM_001145526.2 [Q63HM1-2]
UniGeneiHs.558614

Genome annotation databases

EnsembliENST00000327898; ENSP00000328938; ENSG00000183077 [Q63HM1-2]
ENST00000409257; ENSP00000386890; ENSG00000183077 [Q63HM1-1]
GeneIDi125061
KEGGihsa:125061
UCSCiuc002juz.4 human [Q63HM1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX648442 mRNA Translation: CAH56149.1
BC132824 mRNA Translation: AAI32825.1
CCDSiCCDS32750.2 [Q63HM1-1]
CCDS45801.1 [Q63HM1-2]
RefSeqiNP_001010982.2, NM_001010982.4 [Q63HM1-1]
NP_001138998.1, NM_001145526.2 [Q63HM1-2]
UniGeneiHs.558614

3D structure databases

ProteinModelPortaliQ63HM1
SMRiQ63HM1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125914, 6 interactors
IntActiQ63HM1, 2 interactors
STRINGi9606.ENSP00000328938

Protein family/group databases

ESTHERihuman-AFMID Kynurenine-formamidase
MEROPSiS09.977

PTM databases

iPTMnetiQ63HM1
PhosphoSitePlusiQ63HM1

Polymorphism and mutation databases

BioMutaiAFMID
DMDMi259016175

Proteomic databases

EPDiQ63HM1
MaxQBiQ63HM1
PaxDbiQ63HM1
PeptideAtlasiQ63HM1
PRIDEiQ63HM1
ProteomicsDBi65879
65880 [Q63HM1-2]

Protocols and materials databases

DNASUi125061
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000327898; ENSP00000328938; ENSG00000183077 [Q63HM1-2]
ENST00000409257; ENSP00000386890; ENSG00000183077 [Q63HM1-1]
GeneIDi125061
KEGGihsa:125061
UCSCiuc002juz.4 human [Q63HM1-1]

Organism-specific databases

CTDi125061
EuPathDBiHostDB:ENSG00000183077.15
GeneCardsiAFMID
HGNCiHGNC:20910 AFMID
HPAiHPA023861
HPA026536
neXtProtiNX_Q63HM1
OpenTargetsiENSG00000183077
PharmGKBiPA134958475
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4627 Eukaryota
COG0657 LUCA
GeneTreeiENSGT00390000011093
HOGENOMiHOG000260457
HOVERGENiHBG100436
InParanoidiQ63HM1
KOiK01432
OMAiKEESGFM
OrthoDBiEOG091G0KT8
PhylomeDBiQ63HM1
TreeFamiTF315112

Enzyme and pathway databases

UniPathwayi
UPA00333;UER00454

ReactomeiR-HSA-71240 Tryptophan catabolism

Miscellaneous databases

ChiTaRSiAFMID human
GenomeRNAii125061
PROiPR:Q63HM1

Gene expression databases

BgeeiENSG00000183077 Expressed in 113 organ(s), highest expression level in right lobe of liver
CleanExiHS_AFMID
ExpressionAtlasiQ63HM1 baseline and differential

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
HAMAPiMF_03014 KFase, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR013094 AB_hydrolase_3
IPR027519 KFase_ver/fungi-typ
PfamiView protein in Pfam
PF07859 Abhydrolase_3, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKFA_HUMAN
AccessioniPrimary (citable) accession number: Q63HM1
Secondary accession number(s): A2RUB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 22, 2009
Last modified: November 7, 2018
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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