AFMID

Functionⁱ

Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.UniRule annotation

Catalytic activityⁱ

N-formyl-L-kynurenine + H₂O = formate + L-kynurenine.UniRule annotation

Pathwayⁱ: L-tryptophan degradation via kynurenine pathway

This protein is involved in step 2 of the subpathway that synthesizes L-kynurenine from L-tryptophan.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

Indoleamine 2,3-dioxygenase 1 (IDO1), Indoleamine 2,3-dioxygenase 2 (IDO2), Tryptophan 2,3-dioxygenase (TDO2)
Kynurenine formamidase (AFMID)

This subpathway is part of the pathway L-tryptophan degradation via kynurenine pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-kynurenine from L-tryptophan, the pathway L-tryptophan degradation via kynurenine pathway and in Amino-acid degradation.

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	164	NucleophileUniRule annotation	1
Active siteⁱ	247	UniRule annotation	1
Active siteⁱ	279	UniRule annotation	1

GO - Molecular functionⁱ

arylformamidase activity Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

'de novo' NAD biosynthetic process from tryptophan Source: UniProtKB-UniRule
tryptophan catabolic process to kynurenine
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase
Biological process	Tryptophan catabolism

Enzyme and pathway databases

Reactomeⁱ	R-HSA-71240 Tryptophan catabolism
UniPathwayⁱ	UPA00333;UER00454

Protein family/group databases

ESTHERⁱ	human-AFMID Kynurenine-formamidase
MEROPS protease database More...MEROPSⁱ	S09.977

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Kynurenine formamidaseUniRule annotation (EC:3.5.1.9UniRule annotation) Short name: KFAUniRule annotation Short name: KFaseUniRule annotation Alternative name(s): ArylformamidaseUniRule annotation N-formylkynurenine formamidaseUniRule annotation Short name: FKFUniRule annotation
Gene namesⁱ	Name:AFMIDUniRule annotation
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 17

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000183077.15
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:20910 AFMID
neXtProtⁱ	NX_Q63HM1

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Organism-specific databases

Open Targets More...OpenTargetsⁱ	ENSG00000183077
PharmGKBⁱ	PA134958475

Polymorphism and mutation databases

BioMutaⁱ	AFMID
DMDMⁱ	259016175

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000248308	1 – 303	Kynurenine formamidaseAdd BLAST		303

Proteomic databases

EPDⁱ	Q63HM1
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q63HM1
PaxDbⁱ	Q63HM1
PeptideAtlas More...PeptideAtlasⁱ	Q63HM1
PRIDEⁱ	Q63HM1
ProteomicsDBⁱ	65879 65880 [Q63HM1-2]

PTM databases

iPTMnetⁱ	Q63HM1
PhosphoSitePlusⁱ	Q63HM1

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000183077 Expressed in 113 organ(s), highest expression level in right lobe of liver
CleanExⁱ	HS_AFMID
ExpressionAtlasⁱ	Q63HM1 baseline and differential

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA023861 HPA026536

HPAⁱ

HPA023861
HPA026536

Interactionⁱ

Subunit structureⁱ

Homodimer.UniRule annotation

Protein-protein interaction databases

BioGridⁱ	125914, 6 interactors
IntActⁱ	Q63HM1, 2 interactors
STRINGⁱ	9606.ENSP00000328938

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q63HM1
SMRⁱ	Q63HM1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	95 – 99	HGGXW		5

Domainⁱ

The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.UniRule annotation

Sequence similaritiesⁱ

Belongs to the kynurenine formamidase family.UniRule annotation

Phylogenomic databases

eggNOGⁱ	KOG4627 Eukaryota COG0657 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000011093
HOGENOMⁱ	HOG000260457
HOVERGENⁱ	HBG100436
InParanoidⁱ	Q63HM1
KEGG Orthology (KO) More...KOⁱ	K01432
OMAⁱ	KEESGFM
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0KT8
PhylomeDBⁱ	Q63HM1
TreeFamⁱ	TF315112

Family and domain databases

Gene3Dⁱ	3.40.50.1820, 1 hit
HAMAPⁱ	MF_03014 KFase, 1 hit
InterProⁱ	View protein in InterPro IPR029058 AB_hydrolase IPR013094 AB_hydrolase_3 IPR027519 KFase_ver/fungi-typ
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07859 Abhydrolase_3, 1 hit
SUPFAMⁱ	SSF53474 SSF53474, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 8 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q63HM1-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMDVSGVGFP SKVPWKKMSA EELENQYCPS RWVVRLGAEE ALRTYSQIGI 
        60         70         80         90        100
EATTRARATR KSLLHVPYGD GEGEKVDIYF PDESSEALPF FLFFHGGYWQ 
       110        120        130        140        150
SGSKDESAFM VHPLTAQGVA VVIVAYGIAP KGTLDHMVDQ VTRSVAFVQK 
       160        170        180        190        200
RYPSNKGIYL CGHSAGAHLA AMMLLADWTK HGVTPNLRGF FLVSGVFDLE 
       210        220        230        240        250
PIVYTSQNVA LQLTLEDAQR NSPQLKVAQA QPVDPTCRVL VVVGQFDSPE 
       260        270        280        290        300
FHRQSWEFYQ TLCQGEWKAS FEELHDVDHF EIVENLTQKD NVLTQIILKT 

IFQ

Length:303

Mass (Da):33,992

Last modified:September 22, 2009 - v2

Checksum:ⁱ6E329218366C027D

GO

Isoform 2 (identifier: Q63HM1-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
260-260: Q → QVLPVQ

« Hide

        10         20         30         40         50
MMDVSGVGFP SKVPWKKMSA EELENQYCPS RWVVRLGAEE ALRTYSQIGI 
        60         70         80         90        100
EATTRARATR KSLLHVPYGD GEGEKVDIYF PDESSEALPF FLFFHGGYWQ 
       110        120        130        140        150
SGSKDESAFM VHPLTAQGVA VVIVAYGIAP KGTLDHMVDQ VTRSVAFVQK 
       160        170        180        190        200
RYPSNKGIYL CGHSAGAHLA AMMLLADWTK HGVTPNLRGF FLVSGVFDLE 
       210        220        230        240        250
PIVYTSQNVA LQLTLEDAQR NSPQLKVAQA QPVDPTCRVL VVVGQFDSPE 
       260        270        280        290        300
FHRQSWEFYQ VLPVQTLCQG EWKASFEELH DVDHFEIVEN LTQKDNVLTQ 

IILKTIFQ

Note: No experimental confirmation available.

Show »

Length:308

Mass (Da):34,528

Checksum:ⁱ06640990D1E1B57C

GO

Computationally mapped potential isoform sequencesⁱ

There are 8 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

K7EMI4	K7EMI4_HUMAN	Kynurenine formamidase Kynurenine formamidase	AFMID	206	Annotation score:
K7EMM5	K7EMM5_HUMAN	Kynurenine formamidase Kynurenine formamidase	AFMID	187	Annotation score:
K7EK09	K7EK09_HUMAN	Kynurenine formamidase Kynurenine formamidase	AFMID	111	Annotation score:
W4VSQ7	W4VSQ7_HUMAN	Arylformamidase, isoform CRA_b Arylformamidase, isoform CRA_b (Kynurenine formamidase)	AFMID hCG_29397	57	Annotation score:
K7ELV9	K7ELV9_HUMAN	Kynurenine formamidase Kynurenine formamidase	AFMID	37	Annotation score:
K7EQK5	K7EQK5_HUMAN	Kynurenine formamidase Kynurenine formamidase	AFMID	60	Annotation score:
K7EPF8	K7EPF8_HUMAN	Kynurenine formamidase Kynurenine formamidase	AFMID	84	Annotation score:
K7EIX3	K7EIX3_HUMAN	Kynurenine formamidase Kynurenine formamidase	AFMID	93	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	222	S → N in CAH56149 (PubMed:17974005).Curated		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_038002	260	Q → QVLPVQ in isoform 2. 1 Publication		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BX648442 mRNA Translation: CAH56149.1 BC132824 mRNA Translation: AAI32825.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS32750.2 [Q63HM1-1] CCDS45801.1 [Q63HM1-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_001010982.2, NM_001010982.4 [Q63HM1-1] NP_001138998.1, NM_001145526.2 [Q63HM1-2]
UniGeneⁱ	Hs.558614

Genome annotation databases

Ensemblⁱ	ENST00000327898; ENSP00000328938; ENSG00000183077 [Q63HM1-2] ENST00000409257; ENSP00000386890; ENSG00000183077 [Q63HM1-1]
GeneIDⁱ	125061
KEGGⁱ	hsa:125061
UCSC genome browser More...UCSCⁱ	uc002juz.4 human [Q63HM1-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q63HM1	Kynurenine formamidase		PANTR		308	UniRef90_Q63HM1
Kynurenine formamidase		PONAB		308
Isoform 2 of Kynurenine formamidase		HUMAN		308
Kynurenine formamidase		GORGO		304
UPI000DC16D49		THEGE		273
+5

Protein	Similar proteins		Species	Score	Length	Source
Q63HM1	Kynurenine formamidase		PONAB		308	UniRef50_Q63HM1
Isoform 2 of Kynurenine formamidase		HUMAN		308
Kynurenine formamidase		PANTR		308
Kynurenine formamidase		GORGO		304
Kynurenine formamidase		ICTTR		306
+65

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BX648442 mRNA Translation: CAH56149.1 BC132824 mRNA Translation: AAI32825.1
CCDSⁱ	CCDS32750.2 [Q63HM1-1] CCDS45801.1 [Q63HM1-2]
RefSeqⁱ	NP_001010982.2, NM_001010982.4 [Q63HM1-1] NP_001138998.1, NM_001145526.2 [Q63HM1-2]
UniGeneⁱ	Hs.558614

3D structure databases

ProteinModelPortalⁱ	Q63HM1
SMRⁱ	Q63HM1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	125914, 6 interactors
IntActⁱ	Q63HM1, 2 interactors
STRINGⁱ	9606.ENSP00000328938

Protein family/group databases

ESTHERⁱ	human-AFMID Kynurenine-formamidase
MEROPSⁱ	S09.977

PTM databases

iPTMnetⁱ	Q63HM1
PhosphoSitePlusⁱ	Q63HM1

Polymorphism and mutation databases

BioMutaⁱ	AFMID
DMDMⁱ	259016175

Proteomic databases

EPDⁱ	Q63HM1
MaxQBⁱ	Q63HM1
PaxDbⁱ	Q63HM1
PeptideAtlasⁱ	Q63HM1
PRIDEⁱ	Q63HM1
ProteomicsDBⁱ	65879 65880 [Q63HM1-2]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	125061
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000327898; ENSP00000328938; ENSG00000183077 [Q63HM1-2] ENST00000409257; ENSP00000386890; ENSG00000183077 [Q63HM1-1]
GeneIDⁱ	125061
KEGGⁱ	hsa:125061
UCSCⁱ	uc002juz.4 human [Q63HM1-1]

Organism-specific databases

CTDⁱ	125061
EuPathDBⁱ	HostDB:ENSG00000183077.15
GeneCardsⁱ	AFMID
HGNCⁱ	HGNC:20910 AFMID
HPAⁱ	HPA023861 HPA026536
neXtProtⁱ	NX_Q63HM1
OpenTargetsⁱ	ENSG00000183077
PharmGKBⁱ	PA134958475
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG4627 Eukaryota COG0657 LUCA
GeneTreeⁱ	ENSGT00390000011093
HOGENOMⁱ	HOG000260457
HOVERGENⁱ	HBG100436
InParanoidⁱ	Q63HM1
KOⁱ	K01432
OMAⁱ	KEESGFM
OrthoDBⁱ	EOG091G0KT8
PhylomeDBⁱ	Q63HM1
TreeFamⁱ	TF315112

Enzyme and pathway databases

UniPathwayⁱ	UPA00333;UER00454
Reactomeⁱ	R-HSA-71240 Tryptophan catabolism

Miscellaneous databases

ChiTaRSⁱ	AFMID human
GenomeRNAiⁱ	125061
Protein Ontology More...PROⁱ	PR:Q63HM1

Gene expression databases

Bgeeⁱ	ENSG00000183077 Expressed in 113 organ(s), highest expression level in right lobe of liver
CleanExⁱ	HS_AFMID
ExpressionAtlasⁱ	Q63HM1 baseline and differential

Family and domain databases

Gene3Dⁱ	3.40.50.1820, 1 hit
HAMAPⁱ	MF_03014 KFase, 1 hit
InterProⁱ	View protein in InterPro IPR029058 AB_hydrolase IPR013094 AB_hydrolase_3 IPR027519 KFase_ver/fungi-typ
Pfamⁱ	View protein in Pfam PF07859 Abhydrolase_3, 1 hit
SUPFAMⁱ	SSF53474 SSF53474, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	KFA_HUMAN
Accessionⁱ	Q63HM1Primary (citable) accession number: Q63HM1 Secondary accession number(s): A2RUB3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
	Last sequence update:	September 22, 2009
	Last modified:	November 7, 2018
	This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways
Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM

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UniProtKB - Q63HM1 (KFA_HUMAN)

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Kynurenine formamidase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-tryptophan degradation via kynurenine pathway

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytosol

Nucleus

Cytosol

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

Pathwayⁱ: L-tryptophan degradation via kynurenine pathway

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ