UniProtKB - Q63HM1 (KFA_HUMAN)
Protein
Kynurenine formamidase
Gene
AFMID
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.UniRule annotation
Catalytic activityi
- EC:3.5.1.9UniRule annotation
: L-tryptophan degradation via kynurenine pathway Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes L-kynurenine from L-tryptophan.UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Indoleamine 2,3-dioxygenase 2 (IDO2), Indoleamine 2,3-dioxygenase 1 (IDO1), Tryptophan 2,3-dioxygenase (TDO2)
- Kynurenine formamidase (AFMID)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-kynurenine from L-tryptophan, the pathway L-tryptophan degradation via kynurenine pathway and in Amino-acid degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 164 | NucleophileUniRule annotation | 1 | |
Active sitei | 247 | UniRule annotation | 1 | |
Active sitei | 279 | UniRule annotation | 1 |
GO - Molecular functioni
- arylformamidase activity Source: GO_Central
- hydrolase activity Source: GO_Central
GO - Biological processi
- 'de novo' NAD biosynthetic process from tryptophan Source: UniProtKB-UniRule
- NAD biosynthetic process Source: GO_Central
- tryptophan catabolic process to kynurenine Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Biological process | Tryptophan catabolism |
Enzyme and pathway databases
PathwayCommonsi | Q63HM1 |
Reactomei | R-HSA-71240, Tryptophan catabolism |
UniPathwayi | UPA00333;UER00454 |
Protein family/group databases
ESTHERi | human-AFMID, Kynurenine-formamidase |
MEROPSi | S09.977 |
Names & Taxonomyi
Protein namesi | Recommended name: Kynurenine formamidaseUniRule annotation (EC:3.5.1.9UniRule annotation)Short name: KFAUniRule annotation Short name: KFaseUniRule annotation Alternative name(s): ArylformamidaseUniRule annotation N-formylkynurenine formamidaseUniRule annotation Short name: FKFUniRule annotation |
Gene namesi | Name:AFMIDUniRule annotation |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000183077.15 |
HGNCi | HGNC:20910, AFMID |
neXtProti | NX_Q63HM1 |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 125061 |
OpenTargetsi | ENSG00000183077 |
PharmGKBi | PA134958475 |
Miscellaneous databases
Pharosi | Q63HM1, Tbio |
Polymorphism and mutation databases
BioMutai | AFMID |
DMDMi | 259016175 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000248308 | 1 – 303 | Kynurenine formamidaseAdd BLAST | 303 |
Proteomic databases
EPDi | Q63HM1 |
MassIVEi | Q63HM1 |
MaxQBi | Q63HM1 |
PaxDbi | Q63HM1 |
PeptideAtlasi | Q63HM1 |
PRIDEi | Q63HM1 |
ProteomicsDBi | 65879 [Q63HM1-1] 65880 [Q63HM1-2] |
PTM databases
iPTMneti | Q63HM1 |
PhosphoSitePlusi | Q63HM1 |
Expressioni
Gene expression databases
Bgeei | ENSG00000183077, Expressed in right lobe of liver and 135 other tissues |
ExpressionAtlasi | Q63HM1, baseline and differential |
Organism-specific databases
HPAi | ENSG00000183077, Tissue enhanced (liver) |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationBinary interactionsi
Hide detailsQ63HM1
With | #Exp. | IntAct |
---|---|---|
DDIT4L [Q96D03] | 5 | EBI-13286382,EBI-742054 |
MED18 [Q9BUE0] | 3 | EBI-13286382,EBI-394640 |
MEI4 [A8MW99] | 3 | EBI-13286382,EBI-19944212 |
Protein-protein interaction databases
BioGRIDi | 125914, 11 interactors |
IntActi | Q63HM1, 4 interactors |
STRINGi | 9606.ENSP00000328938 |
Miscellaneous databases
RNActi | Q63HM1, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 95 – 99 | HGGXW | 5 |
Domaini
The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.UniRule annotation
Sequence similaritiesi
Belongs to the kynurenine formamidase family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG4627, Eukaryota |
GeneTreei | ENSGT00390000011093 |
HOGENOMi | CLU_012494_4_7_1 |
InParanoidi | Q63HM1 |
OMAi | WSHRMAA |
OrthoDBi | 992858at2759 |
PhylomeDBi | Q63HM1 |
TreeFami | TF315112 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
HAMAPi | MF_03014, KFase, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR013094, AB_hydrolase_3 IPR027519, KFase_ver/fungi-typ |
Pfami | View protein in Pfam PF07859, Abhydrolase_3, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q63HM1-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MMDVSGVGFP SKVPWKKMSA EELENQYCPS RWVVRLGAEE ALRTYSQIGI
60 70 80 90 100
EATTRARATR KSLLHVPYGD GEGEKVDIYF PDESSEALPF FLFFHGGYWQ
110 120 130 140 150
SGSKDESAFM VHPLTAQGVA VVIVAYGIAP KGTLDHMVDQ VTRSVAFVQK
160 170 180 190 200
RYPSNKGIYL CGHSAGAHLA AMMLLADWTK HGVTPNLRGF FLVSGVFDLE
210 220 230 240 250
PIVYTSQNVA LQLTLEDAQR NSPQLKVAQA QPVDPTCRVL VVVGQFDSPE
260 270 280 290 300
FHRQSWEFYQ TLCQGEWKAS FEELHDVDHF EIVENLTQKD NVLTQIILKT
IFQ
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EMI4 | K7EMI4_HUMAN | Kynurenine formamidase | AFMID | 206 | Annotation score: | ||
K7EMM5 | K7EMM5_HUMAN | Kynurenine formamidase | AFMID | 187 | Annotation score: | ||
K7EK09 | K7EK09_HUMAN | Kynurenine formamidase | AFMID | 111 | Annotation score: | ||
K7ELV9 | K7ELV9_HUMAN | Kynurenine formamidase | AFMID | 37 | Annotation score: | ||
K7EIX3 | K7EIX3_HUMAN | Kynurenine formamidase | AFMID | 93 | Annotation score: | ||
K7EQK5 | K7EQK5_HUMAN | Kynurenine formamidase | AFMID | 60 | Annotation score: | ||
K7EPF8 | K7EPF8_HUMAN | Kynurenine formamidase | AFMID | 84 | Annotation score: | ||
W4VSQ7 | W4VSQ7_HUMAN | Arylformamidase, isoform CRA_b | AFMID hCG_29397 | 57 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 222 | S → N in CAH56149 (PubMed:17974005).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_038002 | 260 | Q → QVLPVQ in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX648442 mRNA Translation: CAH56149.1 BC132824 mRNA Translation: AAI32825.1 |
CCDSi | CCDS32750.2 [Q63HM1-1] CCDS45801.1 [Q63HM1-2] |
RefSeqi | NP_001010982.2, NM_001010982.4 [Q63HM1-1] NP_001138998.1, NM_001145526.2 [Q63HM1-2] |
Genome annotation databases
Ensembli | ENST00000327898; ENSP00000328938; ENSG00000183077 [Q63HM1-2] ENST00000409257; ENSP00000386890; ENSG00000183077 [Q63HM1-1] |
GeneIDi | 125061 |
KEGGi | hsa:125061 |
UCSCi | uc002juz.4, human [Q63HM1-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX648442 mRNA Translation: CAH56149.1 BC132824 mRNA Translation: AAI32825.1 |
CCDSi | CCDS32750.2 [Q63HM1-1] CCDS45801.1 [Q63HM1-2] |
RefSeqi | NP_001010982.2, NM_001010982.4 [Q63HM1-1] NP_001138998.1, NM_001145526.2 [Q63HM1-2] |
3D structure databases
SMRi | Q63HM1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 125914, 11 interactors |
IntActi | Q63HM1, 4 interactors |
STRINGi | 9606.ENSP00000328938 |
Protein family/group databases
ESTHERi | human-AFMID, Kynurenine-formamidase |
MEROPSi | S09.977 |
PTM databases
iPTMneti | Q63HM1 |
PhosphoSitePlusi | Q63HM1 |
Polymorphism and mutation databases
BioMutai | AFMID |
DMDMi | 259016175 |
Proteomic databases
EPDi | Q63HM1 |
MassIVEi | Q63HM1 |
MaxQBi | Q63HM1 |
PaxDbi | Q63HM1 |
PeptideAtlasi | Q63HM1 |
PRIDEi | Q63HM1 |
ProteomicsDBi | 65879 [Q63HM1-1] 65880 [Q63HM1-2] |
Protocols and materials databases
Antibodypediai | 19735, 78 antibodies |
DNASUi | 125061 |
Genome annotation databases
Ensembli | ENST00000327898; ENSP00000328938; ENSG00000183077 [Q63HM1-2] ENST00000409257; ENSP00000386890; ENSG00000183077 [Q63HM1-1] |
GeneIDi | 125061 |
KEGGi | hsa:125061 |
UCSCi | uc002juz.4, human [Q63HM1-1] |
Organism-specific databases
CTDi | 125061 |
DisGeNETi | 125061 |
EuPathDBi | HostDB:ENSG00000183077.15 |
GeneCardsi | AFMID |
HGNCi | HGNC:20910, AFMID |
HPAi | ENSG00000183077, Tissue enhanced (liver) |
neXtProti | NX_Q63HM1 |
OpenTargetsi | ENSG00000183077 |
PharmGKBi | PA134958475 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4627, Eukaryota |
GeneTreei | ENSGT00390000011093 |
HOGENOMi | CLU_012494_4_7_1 |
InParanoidi | Q63HM1 |
OMAi | WSHRMAA |
OrthoDBi | 992858at2759 |
PhylomeDBi | Q63HM1 |
TreeFami | TF315112 |
Enzyme and pathway databases
UniPathwayi | UPA00333;UER00454 |
PathwayCommonsi | Q63HM1 |
Reactomei | R-HSA-71240, Tryptophan catabolism |
Miscellaneous databases
BioGRID-ORCSi | 125061, 3 hits in 846 CRISPR screens |
ChiTaRSi | AFMID, human |
GenomeRNAii | 125061 |
Pharosi | Q63HM1, Tbio |
PROi | PR:Q63HM1 |
RNActi | Q63HM1, protein |
Gene expression databases
Bgeei | ENSG00000183077, Expressed in right lobe of liver and 135 other tissues |
ExpressionAtlasi | Q63HM1, baseline and differential |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
HAMAPi | MF_03014, KFase, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR013094, AB_hydrolase_3 IPR027519, KFase_ver/fungi-typ |
Pfami | View protein in Pfam PF07859, Abhydrolase_3, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KFA_HUMAN | |
Accessioni | Q63HM1Primary (citable) accession number: Q63HM1 Secondary accession number(s): A2RUB3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | September 22, 2009 | |
Last modified: | December 2, 2020 | |
This is version 124 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM