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Protein

1,4-alpha-glucan branching enzyme

Gene

pulA

Organism
Bacillus cereus (strain ZK / E33L)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.SAAS annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidaseImported, Hydrolase, TransferaseSAAS annotation

Protein family/group databases

CAZyiCBM41 Carbohydrate-Binding Module Family 41
CBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzymeSAAS annotation (EC:2.4.1.18SAAS annotation)
Gene namesi
Name:pulAImported
Ordered Locus Names:BCE33L2463Imported
OrganismiBacillus cereus (strain ZK / E33L)Imported
Taxonomic identifieri288681 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000002612 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_500426697331 – 8501,4-alpha-glucan branching enzymeSequence analysisAdd BLAST820

Structurei

3D structure databases

ProteinModelPortaliQ63AL5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini351 – 750AamyInterPro annotationAdd BLAST400

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.SAAS annotation

Keywords - Domaini

SignalSequence analysis

Phylogenomic databases

KOiK01200
OMAiQMHKLSS

Family and domain databases

CDDicd10315 CBM41_pullulanase, 1 hit
Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR037439 Branching_enzy
IPR013784 Carb-bd-like_fold
IPR005323 CBM41_pullulanase
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR011840 PulA_typeI
PfamiView protein in Pfam
PF00128 Alpha-amylase, 2 hits
PF02922 CBM_48, 1 hit
PF03714 PUD, 1 hit
PIRSFiPIRSF000463 GlgB, 2 hits
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF49452 SSF49452, 1 hit
SSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit
TIGRFAMsiTIGR02104 pulA_typeI, 1 hit

Sequencei

Sequence statusi: Complete.

Q63AL5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTKRLINKSV LLLTIIVMLS SVYSFQNVKA VSNSKTTEVI IHYKEQSGNT
60 70 80 90 100
KDWNLWIWGE NSNGKSYEFT GEDEFGKYAK INIDGDYNRL GFIIRTSEWE
110 120 130 140 150
KDGGDRWIEN IKDGRAEVWI LSGDEKVYNS KPSSDLSIQK ATIDSFHEIT
160 170 180 190 200
VTTNVPFHIK EKKIEMEGIK IKSISPYDIN SGDITNKVKI ITDQKIDLKQ
210 220 230 240 250
TYKVKIENLA DTNTEIGKVI RTEEFDKLFY YGGNDLGNIY TPQHTKFRVW
260 270 280 290 300
APTASEAKLV TYKKWNDKIG TEINMQQGEK GTWKAELKGN QKGLYYTYKV
310 320 330 340 350
KIGDKWTEAV DPYARAASVN GDKGAVVDLE ETNPKRWNTN KKPKLKNPED
360 370 380 390 400
AIIYELHVRD LSIQPESGIK QKGKYLGVTE KGTKGPEGVK TGLDHMKDLG
410 420 430 440 450
VTHVQLLPIF DYASVNEEKV NEPQYNWGYD PKNFNVPEGS YSTNPYEPTV
460 470 480 490 500
RITELKQMIQ TLHDNNLRVV MDVVYNHMYN AAESNFHKLV PGYYYRYNED
510 520 530 540 550
GTFANGTGVG NDTASERKMM RKFMIDSVTY WAKEYNLDGF RFDLMGIHDY
560 570 580 590 600
ETMNEIRKAV NQIDPSIILH GEGWDLNTPL AAELKANQKN AEKMKGIAHF
610 620 630 640 650
NDNIRDGLKG SVFEEKENGF VNGKENMEDR IKKGITAGID YDTNSSTYLD
660 670 680 690 700
PEQVLTYVEA HDNHTLWDKL ELTNPGDSEE ARKQMHKLSS SILLTSQGIP
710 720 730 740 750
FLHAGQEFMR TKYGDHNSYK SPDSINQMDW LRRAAFNNEV DYMKGLIELR
760 770 780 790 800
KKYPAFRMTS AEQIKTHVSF IDAPKNTVAY TIEGNKNEYF TVAHNANRES
810 820 830 840 850
VEISLPSKGP WKVLVDGKQA GSKPLYVVHD NKIKVPALSS LVLKTEKPIK
Length:850
Mass (Da):97,018
Last modified:October 25, 2004 - v1
Checksum:iFC30B9E09D1D3C8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000001 Genomic DNA Translation: AAU17796.1

Genome annotation databases

EnsemblBacteriaiAAU17796; AAU17796; BCE33L2463
KEGGibcz:BCE33L2463

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000001 Genomic DNA Translation: AAU17796.1

3D structure databases

ProteinModelPortaliQ63AL5
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM41 Carbohydrate-Binding Module Family 41
CBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU17796; AAU17796; BCE33L2463
KEGGibcz:BCE33L2463

Phylogenomic databases

KOiK01200
OMAiQMHKLSS

Family and domain databases

CDDicd10315 CBM41_pullulanase, 1 hit
Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR037439 Branching_enzy
IPR013784 Carb-bd-like_fold
IPR005323 CBM41_pullulanase
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR011840 PulA_typeI
PfamiView protein in Pfam
PF00128 Alpha-amylase, 2 hits
PF02922 CBM_48, 1 hit
PF03714 PUD, 1 hit
PIRSFiPIRSF000463 GlgB, 2 hits
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF49452 SSF49452, 1 hit
SSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit
TIGRFAMsiTIGR02104 pulA_typeI, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiQ63AL5_BACCZ
AccessioniPrimary (citable) accession number: Q63AL5
Entry historyiIntegrated into UniProtKB/TrEMBL: October 25, 2004
Last sequence update: October 25, 2004
Last modified: February 28, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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