UniProtKB - Q63961 (EGLN_MOUSE)
Endoglin
Eng
Functioni
Miscellaneous
GO - Molecular functioni
- BMP binding Source: Ensembl
- coreceptor activity Source: MGI
- galactose binding Source: MGI
- glycosaminoglycan binding Source: MGI
- identical protein binding Source: MGI
- protein homodimerization activity Source: BHF-UCL
- transforming growth factor beta-activated receptor activity Source: GO_Central
- transforming growth factor beta binding Source: BHF-UCL
- type II transforming growth factor beta receptor binding Source: MGI
- type I transforming growth factor beta receptor binding Source: MGI
GO - Biological processi
- angiogenesis Source: MGI
- artery morphogenesis Source: BHF-UCL
- atrial cardiac muscle tissue morphogenesis Source: BHF-UCL
- atrioventricular canal morphogenesis Source: BHF-UCL
- branching involved in blood vessel morphogenesis Source: BHF-UCL
- cardiac atrium morphogenesis Source: BHF-UCL
- cardiac ventricle morphogenesis Source: BHF-UCL
- cell adhesion Source: UniProtKB-KW
- cell chemotaxis Source: MGI
- cell migration Source: MGI
- cell migration involved in endocardial cushion formation Source: MGI
- cell motility Source: MGI
- central nervous system vasculogenesis Source: MGI
- chronological cell aging Source: BHF-UCL
- detection of hypoxia Source: MGI
- dorsal aorta morphogenesis Source: BHF-UCL
- endocardial cushion morphogenesis Source: BHF-UCL
- epithelial to mesenchymal transition Source: GO_Central
- epithelial to mesenchymal transition involved in endocardial cushion formation Source: BHF-UCL
- extracellular matrix constituent secretion Source: MGI
- extracellular matrix disassembly Source: MGI
- heart development Source: MGI
- heart looping Source: BHF-UCL
- negative regulation of cell migration Source: MGI
- negative regulation of endothelial cell proliferation Source: MGI
- negative regulation of gene expression Source: BHF-UCL
- negative regulation of nitric-oxide synthase activity Source: MGI
- negative regulation of pathway-restricted SMAD protein phosphorylation Source: MGI
- negative regulation of protein autophosphorylation Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- outflow tract septum morphogenesis Source: BHF-UCL
- positive regulation of angiogenesis Source: MGI
- positive regulation of BMP signaling pathway Source: MGI
- positive regulation of collagen biosynthetic process Source: MGI
- positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation Source: BHF-UCL
- positive regulation of gene expression Source: MGI
- positive regulation of pathway-restricted SMAD protein phosphorylation Source: MGI
- positive regulation of protein kinase B signaling Source: MGI
- positive regulation of protein phosphorylation Source: MGI
- positive regulation of systemic arterial blood pressure Source: MGI
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- positive regulation of vascular associated smooth muscle cell differentiation Source: BHF-UCL
- regulation of transcription, DNA-templated Source: MGI
- regulation of transforming growth factor beta receptor signaling pathway Source: MGI
- response to hypoxia Source: MGI
- smooth muscle tissue development Source: BHF-UCL
- transforming growth factor beta receptor signaling pathway Source: MGI
- vascular associated smooth muscle cell development Source: BHF-UCL
- vasculogenesis Source: BHF-UCL
- venous blood vessel morphogenesis Source: BHF-UCL
- ventricular trabecula myocardium morphogenesis Source: BHF-UCL
- wound healing Source: MGI
Keywordsi
Biological process | Angiogenesis, Cell adhesion |
Names & Taxonomyi
Protein namesi | Recommended name: EndoglinAlternative name(s): Cell surface MJ7/18 antigen CD_antigen: CD1051 Publication |
Gene namesi | Name:Eng Synonyms:Edg |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95392, Eng |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication
Extracellular region or secreted
- endothelial microparticle Source: BHF-UCL
- extracellular space Source: MGI
Nucleus
- nuclear body Source: MGI
Plasma Membrane
- external side of plasma membrane Source: MGI
- plasma membrane Source: MGI
Other locations
- cell surface Source: MGI
- integral component of membrane Source: UniProtKB-KW
- receptor complex Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 27 – 581 | ExtracellularSequence analysisAdd BLAST | 555 | |
Transmembranei | 582 – 606 | HelicalSequence analysisAdd BLAST | 25 | |
Topological domaini | 607 – 653 | CytoplasmicSequence analysisAdd BLAST | 47 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | Sequence analysisAdd BLAST | 26 | |
ChainiPRO_0000021157 | 27 – 653 | EndoglinAdd BLAST | 627 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 31 ↔ 209 | By similarity | ||
Disulfide bondi | 54 ↔ 184 | By similarity | ||
Glycosylationi | 89 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 135 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 244 ↔ 330 | By similarity | ||
Glycosylationi | 266 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 307 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 322 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 350 ↔ 382 | By similarity | ||
Disulfide bondi | 363 ↔ 442 | By similarity | ||
Disulfide bondi | 394 ↔ 412 | By similarity | ||
Disulfide bondi | 493 ↔ 549 | By similarity | ||
Disulfide bondi | 516 | InterchainBy similarity | ||
Modified residuei | 641 | Phosphoserine; by TGFBR11 Publication | 1 | |
Modified residuei | 644 | Phosphoserine; by TGFBR11 Publication | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
EPDi | Q63961 |
jPOSTi | Q63961 |
MaxQBi | Q63961 |
PaxDbi | Q63961 |
PRIDEi | Q63961 |
PTM databases
GlyGeni | Q63961, 5 sites |
iPTMneti | Q63961 |
PhosphoSitePlusi | Q63961 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000026814, Expressed in heart and 297 other tissues |
ExpressionAtlasi | Q63961, baseline and differential |
Genevisiblei | Q63961, MM |
Interactioni
Subunit structurei
Homodimer; disulfide-linked (PubMed:8194490).
Forms a heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2.
Interacts with TGFB1 (PubMed:8194490). It is able to bind TGFB1 and TGFB2 with high affinity, but not TGFB3.
Interacts with GDF2, forming a heterotetramer with a 2:2 stoichiometry.
Interacts with ACVRL1. Can form a heteromeric complex with GDF2 and ACVRL1.
Interacts with BMP10.
Interacts with TCTEX1D4.
Interacts with ARRB2.
By similarity1 PublicationGO - Molecular functioni
- BMP binding Source: Ensembl
- identical protein binding Source: MGI
- protein homodimerization activity Source: BHF-UCL
- transforming growth factor beta binding Source: BHF-UCL
- type II transforming growth factor beta receptor binding Source: MGI
- type I transforming growth factor beta receptor binding Source: MGI
Protein-protein interaction databases
DIPi | DIP-47636N |
IntActi | Q63961, 7 interactors |
MINTi | Q63961 |
STRINGi | 10090.ENSMUSP00000009705 |
Miscellaneous databases
RNActi | Q63961, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 363 – 510 | ZPPROSITE-ProRule annotationAdd BLAST | 148 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 27 – 337 | Required for interaction with GDF2By similarityAdd BLAST | 311 | |
Regioni | 27 – 47 | OR1, N-terminal partBy similarityAdd BLAST | 21 | |
Regioni | 48 – 201 | OR2By similarityAdd BLAST | 154 | |
Regioni | 202 – 330 | OR1, C-terminal partBy similarityAdd BLAST | 129 | |
Regioni | 270 – 282 | Essential for interaction with GDF2By similarityAdd BLAST | 13 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 336 – 574 | Ser/Thr-richAdd BLAST | 239 |
Domaini
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502RZQ9, Eukaryota |
GeneTreei | ENSGT00530000063861 |
InParanoidi | Q63961 |
OrthoDBi | 1263397at2759 |
TreeFami | TF337375 |
Family and domain databases
InterProi | View protein in InterPro IPR001507, ZP_dom |
Pfami | View protein in Pfam PF00100, Zona_pellucida, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MDRGVLPLPI TLLFVIYSFV PTTGLAERVG CDLQPVDPTR GEVTFTTSQV
60 70 80 90 100
SEGCVAQAAN AVREVHVLFL DFPGMLSHLE LTLQASKQNG TETQEVFLVL
110 120 130 140 150
VSNKNVFVKF QAPEIPLHLA YDSSLVIFQG QPRVNITVLP SLTSRKQILD
160 170 180 190 200
WAATKGAITS IAALDDPQSI VLQLGQDPKA PFLCLPEAHK DMGATLEWQP
210 220 230 240 250
RAQTPVQSCR LEGVSGHKEA YILRILPGSE AGPRTVTVMM ELSCTSGDAI
260 270 280 290 300
LILHGPPYVS WFIDINHSMQ ILTTGEYSVK IFPGSKVKGV ELPDTPQGLI
310 320 330 340 350
AEARKLNASI VTSFVELPLV SNVSLRASSC GGVFQTTPAP VVTTPPKDTC
360 370 380 390 400
SPVLLMSLIQ PKCGNQVMTL ALNKKHVQTL QCTITGLTFW DSSCQAEDTD
410 420 430 440 450
DHLVLSSAYS SCGMKVTAHV VSNEVIISFP SGSPPLRKKV QCIDMDSLSF
460 470 480 490 500
QLGLYLSPHF LQASNTIELG QQAFVQVSVS PLTSEVTVQL DSCHLDLGPE
510 520 530 540 550
GDMVELIQSR TAKGSCVTLL SPSPEGDPRF SFLLRVYMVP TPTAGTLSCN
560 570 580 590 600
LALRPSTLSQ EVYKTVSMRL NIVSPDLSGK GLVLPSVLGI TFGAFLIGAL
610 620 630 640 650
LTAALWYIYS HTRGPSKREP VVAVAAPASS ESSSTNHSIG STQSTPCSTS
SMA
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF7A1B4 | F7A1B4_MOUSE | Endoglin | Eng | 641 | Annotation score: | ||
Q3UAM9 | Q3UAM9_MOUSE | Endoglin | Eng | 652 | Annotation score: | ||
F7BZW0 | F7BZW0_MOUSE | Endoglin | Eng | 312 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 94 | Q → R in CAA54917 (PubMed:8194490).Curated | 1 | |
Sequence conflicti | 287 | V → D in AAB30196 (PubMed:8125301).Curated | 1 | |
Sequence conflicti | 572 | I → V in CAA54917 (PubMed:8194490).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S69407 mRNA Translation: AAB30196.1 X77952 mRNA Translation: CAA54917.1 AL772271 Genomic DNA No translation available. CH466542 Genomic DNA Translation: EDL08562.1 BC029080 mRNA Translation: AAH29080.1 |
CCDSi | CCDS15925.1 |
PIRi | I48341 |
RefSeqi | NP_031958.2, NM_007932.2 |
Genome annotation databases
Ensembli | ENSMUST00000009705; ENSMUSP00000009705; ENSMUSG00000026814 |
GeneIDi | 13805 |
KEGGi | mmu:13805 |
UCSCi | uc008jgk.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S69407 mRNA Translation: AAB30196.1 X77952 mRNA Translation: CAA54917.1 AL772271 Genomic DNA No translation available. CH466542 Genomic DNA Translation: EDL08562.1 BC029080 mRNA Translation: AAH29080.1 |
CCDSi | CCDS15925.1 |
PIRi | I48341 |
RefSeqi | NP_031958.2, NM_007932.2 |
3D structure databases
SMRi | Q63961 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-47636N |
IntActi | Q63961, 7 interactors |
MINTi | Q63961 |
STRINGi | 10090.ENSMUSP00000009705 |
PTM databases
GlyGeni | Q63961, 5 sites |
iPTMneti | Q63961 |
PhosphoSitePlusi | Q63961 |
Proteomic databases
EPDi | Q63961 |
jPOSTi | Q63961 |
MaxQBi | Q63961 |
PaxDbi | Q63961 |
PRIDEi | Q63961 |
Protocols and materials databases
Antibodypediai | 2321, 2364 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000009705; ENSMUSP00000009705; ENSMUSG00000026814 |
GeneIDi | 13805 |
KEGGi | mmu:13805 |
UCSCi | uc008jgk.2, mouse |
Organism-specific databases
CTDi | 2022 |
MGIi | MGI:95392, Eng |
Phylogenomic databases
eggNOGi | ENOG502RZQ9, Eukaryota |
GeneTreei | ENSGT00530000063861 |
InParanoidi | Q63961 |
OrthoDBi | 1263397at2759 |
TreeFami | TF337375 |
Miscellaneous databases
BioGRID-ORCSi | 13805, 4 hits in 21 CRISPR screens |
ChiTaRSi | Eng, mouse |
PROi | PR:Q63961 |
RNActi | Q63961, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026814, Expressed in heart and 297 other tissues |
ExpressionAtlasi | Q63961, baseline and differential |
Genevisiblei | Q63961, MM |
Family and domain databases
InterProi | View protein in InterPro IPR001507, ZP_dom |
Pfami | View protein in Pfam PF00100, Zona_pellucida, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EGLN_MOUSE | |
Accessioni | Q63961Primary (citable) accession number: Q63961 Secondary accession number(s): Q61520, Q8K100 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 156 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot