UniProtKB - Q63943 (MEF2D_MOUSE)
Myocyte-specific enhancer factor 2D
Mef2d
Functioni
Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT]4TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis.
2 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 58 – 86 | Mef2-typeSequence analysisAdd BLAST | 29 |
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor binding Source: MGI
- enzyme binding Source: BHF-UCL
- histone deacetylase binding Source: MGI
- protein heterodimerization activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- RNA polymerase II-specific DNA-binding transcription factor binding Source: Ensembl
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- adult heart development Source: Ensembl
- apoptotic process Source: UniProtKB-KW
- cell differentiation Source: GO_Central
- chondrocyte differentiation Source: MGI
- endochondral ossification Source: MGI
- nervous system development Source: UniProtKB-KW
- osteoblast differentiation Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- positive regulation of vascular associated smooth muscle cell proliferation Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- skeletal muscle cell differentiation Source: MGI
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Apoptosis, Differentiation, Neurogenesis, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-525793, Myogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: Myocyte-specific enhancer factor 2D |
Gene namesi | Name:Mef2d |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:99533, Mef2d |
VEuPathDBi | HostDB:ENSMUSG00000001419 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation1 Publication
Note: Translocated by HDAC4 to nuclear dots.By similarity
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 20 | T → A: No change in DNA-binding activity. 1 Publication | 1 | |
Mutagenesisi | 20 | T → D: Dramatic decrease in DNA-binding. 1 Publication | 1 | |
Mutagenesisi | 121 | S → A: Abolishes phosphorylation by PKA. No change in protein levels. Loss of protein stability on PKA stimulation. Loss of PKA-mediated repression. No change in interaction with HDAC4 in response to PKA; when associated with A-190. 1 Publication | 1 | |
Mutagenesisi | 190 | S → A: Abolishes phosphorylation by PKA. No change in protein levels. Loss of protein stability on PKA stimulation mediated repression. No change in interaction with HDAC4 in response to PKA; when associated with A-121. 1 Publication | 1 | |
Mutagenesisi | 437 | S → A: Loss of calpain/Cdk5-mediated neuron apoptosis. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000199436 | 1 – 514 | Myocyte-specific enhancer factor 2DAdd BLAST | 514 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 98 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 106 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 110 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 121 | Phosphoserine; by PKA1 Publication | 1 | ||
Modified residuei | 180 | Phosphoserine; by MAPK7By similarity | 1 | ||
Modified residuei | 190 | Phosphoserine; by PKA1 Publication | 1 | ||
Modified residuei | 231 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 245 | N6-acetyllysineBy similarity | 1 | ||
Modified residuei | 251 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 432 | N6-acetyllysine; alternateBy similarity | 1 | ||
Cross-linki | 432 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity | |||
Modified residuei | 437 | Phosphoserine1 Publication | 1 | ||
Isoform Muscle (identifier: Q63943-2) | |||||
Modified residuei | 97 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 107 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 288 – 289 | CleavageCurated | 2 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q63943 |
jPOSTi | Q63943 |
MaxQBi | Q63943 |
PaxDbi | Q63943 |
PRIDEi | Q63943 |
ProteomicsDBi | 295994 [Q63943-1] 295995 [Q63943-2] |
PTM databases
iPTMneti | Q63943 |
PhosphoSitePlusi | Q63943 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000001419, Expressed in retina and 324 other tissues |
Genevisiblei | Q63943, MM |
Interactioni
Subunit structurei
Forms a complex with class II HDACs in undifferentiating cells. On myogenic differentiation, HDACs are released into the cytoplasm allowing MEF2s to interact with other proteins for activation.
Interacts with HDAC4 (in undifferentiating cells); the interaction translocates MEF2D to nuclear dots.
Forms a heterodimer with MEF2A (By similarity).
Interacts with MAPK7; the interaction phosphorylates but does not activate MEF2D (By similarity).
Interacts with MYOG.
Interacts with CCAR2 and HDAC3 (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- DNA-binding transcription factor binding Source: MGI
- enzyme binding Source: BHF-UCL
- histone deacetylase binding Source: MGI
- protein heterodimerization activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- RNA polymerase II-specific DNA-binding transcription factor binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 201384, 8 interactors |
ELMi | Q63943 |
IntActi | Q63943, 5 interactors |
MINTi | Q63943 |
STRINGi | 10090.ENSMUSP00000001455 |
Miscellaneous databases
RNActi | Q63943, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 57 | MADS-boxPROSITE-ProRule annotationAdd BLAST | 55 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 174 – 207 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 244 – 269 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 286 – 292 | Beta domainBy similarity | 7 | |
Regioni | 364 – 399 | DisorderedSequence analysisAdd BLAST | 36 | |
Regioni | 430 – 514 | DisorderedSequence analysisAdd BLAST | 85 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 174 – 196 | Polar residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 368 – 397 | Pro residuesSequence analysisAdd BLAST | 30 | |
Compositional biasi | 440 – 455 | Pro residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0014, Eukaryota |
GeneTreei | ENSGT00940000159463 |
InParanoidi | Q63943 |
OrthoDBi | 729387at2759 |
PhylomeDBi | Q63943 |
Family and domain databases
CDDi | cd00265, MADS_MEF2_like, 1 hit |
Gene3Di | 3.40.1810.10, 1 hit |
InterProi | View protein in InterPro IPR022102, HJURP_C IPR033896, MADS_MEF2-like IPR002100, TF_MADSbox IPR036879, TF_MADSbox_sf |
Pfami | View protein in Pfam PF12347, HJURP_C, 1 hit PF00319, SRF-TF, 1 hit |
PRINTSi | PR00404, MADSDOMAIN |
SMARTi | View protein in SMART SM00432, MADS, 1 hit |
SUPFAMi | SSF55455, SSF55455, 1 hit |
PROSITEi | View protein in PROSITE PS00350, MADS_BOX_1, 1 hit PS50066, MADS_BOX_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGRKKIQIQR ITDERNRQVT FTKRKFGLMK KAYELSVLCD CEIALIIFNH
60 70 80 90 100
SNKLFQYAST DMDKVLLKYT EYNEPHESRT NADIIETLRK KGFNGCDSPE
110 120 130 140 150
PDGEDSLEQS PLLEDKYRRA SEELDGLFRR YGSSVPAPNF AMPVTVPVSN
160 170 180 190 200
QSSMQFSNPS SSLVTPSLVT SSLTDPRLLS PQQPALQRNS VSPGLPQRPA
210 220 230 240 250
SAGAMLGGDL NSANGACPSP VGNGYVSARA SPGLLPVANG NSLNKVIPAK
260 270 280 290 300
SPPPPTHNTQ LGAPSRKPDL RVITSQGGKG LMHHLTEDHL DLNNAQRLGV
310 320 330 340 350
SQSTHSLTTP VVSVATPSLL SQGLPFSSMP TAYNTDYQLP SAELSSLPAF
360 370 380 390 400
SSPAGLALGN VTAWQQPQPP QQPQPPQPPQ SQPQPPQPQP QQPPQQQPHL
410 420 430 440 450
VPVSLSNLIP GSPLPHVGAA LTVTTHPHIS IKSEPVSPSR ERSPAPPPPA
460 470 480 490 500
VFPAARPEPG EGLSSPAGGS YETGDRDDGR GDFGPTLGLL RPAPEPEAEG
510
SAVKRMRLDT WTLK
The sequence of this isoform differs from the canonical sequence as follows:
87-132: TLRKKGFNGC...ELDGLFRRYG → ALHNNDRECE...DKMMQSYRLA
286-292: Missing.
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9QKT0 | E9QKT0_MOUSE | Myocyte-specific enhancer factor 2D | Mef2d | 506 | Annotation score: | ||
Q921S6 | Q921S6_MOUSE | Myocyte enhancer factor 2D | Mef2d | 507 | Annotation score: | ||
E9Q5E0 | E9Q5E0_MOUSE | Myocyte-specific enhancer factor 2D | Mef2d | 506 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 287 | E → G in AAB29973 (PubMed:8114702).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006253 | 87 – 132 | TLRKK…FRRYG → ALHNNDRECESPEVDEAFAL TPQTEEKYKKIDEEKYKKID EEFDKMMQSYRLA in isoform Muscle. 1 PublicationAdd BLAST | 46 | |
Alternative sequenceiVSP_006254 | 286 – 292 | Missing in isoform Muscle. 1 Publication | 7 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S68893 mRNA Translation: AAB29973.1 S68895 mRNA Translation: AAB29974.1 AC137525 Genomic DNA No translation available. |
CCDSi | CCDS79939.1 [Q63943-1] |
PIRi | B56201 |
RefSeqi | NP_001297516.1, NM_001310587.1 [Q63943-1] XP_006501152.1, XM_006501089.2 [Q63943-1] XP_017174961.1, XM_017319472.1 [Q63943-1] |
Genome annotation databases
Ensembli | ENSMUST00000107559; ENSMUSP00000103184; ENSMUSG00000001419 [Q63943-1] |
GeneIDi | 17261 |
KEGGi | mmu:17261 |
UCSCi | uc008pua.1, mouse [Q63943-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S68893 mRNA Translation: AAB29973.1 S68895 mRNA Translation: AAB29974.1 AC137525 Genomic DNA No translation available. |
CCDSi | CCDS79939.1 [Q63943-1] |
PIRi | B56201 |
RefSeqi | NP_001297516.1, NM_001310587.1 [Q63943-1] XP_006501152.1, XM_006501089.2 [Q63943-1] XP_017174961.1, XM_017319472.1 [Q63943-1] |
3D structure databases
SMRi | Q63943 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201384, 8 interactors |
ELMi | Q63943 |
IntActi | Q63943, 5 interactors |
MINTi | Q63943 |
STRINGi | 10090.ENSMUSP00000001455 |
PTM databases
iPTMneti | Q63943 |
PhosphoSitePlusi | Q63943 |
Proteomic databases
EPDi | Q63943 |
jPOSTi | Q63943 |
MaxQBi | Q63943 |
PaxDbi | Q63943 |
PRIDEi | Q63943 |
ProteomicsDBi | 295994 [Q63943-1] 295995 [Q63943-2] |
Protocols and materials databases
Antibodypediai | 1437, 475 antibodies from 35 providers |
DNASUi | 17261 |
Genome annotation databases
Ensembli | ENSMUST00000107559; ENSMUSP00000103184; ENSMUSG00000001419 [Q63943-1] |
GeneIDi | 17261 |
KEGGi | mmu:17261 |
UCSCi | uc008pua.1, mouse [Q63943-1] |
Organism-specific databases
CTDi | 4209 |
MGIi | MGI:99533, Mef2d |
VEuPathDBi | HostDB:ENSMUSG00000001419 |
Phylogenomic databases
eggNOGi | KOG0014, Eukaryota |
GeneTreei | ENSGT00940000159463 |
InParanoidi | Q63943 |
OrthoDBi | 729387at2759 |
PhylomeDBi | Q63943 |
Enzyme and pathway databases
Reactomei | R-MMU-525793, Myogenesis |
Miscellaneous databases
BioGRID-ORCSi | 17261, 2 hits in 64 CRISPR screens |
ChiTaRSi | Mef2d, mouse |
PROi | PR:Q63943 |
RNActi | Q63943, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000001419, Expressed in retina and 324 other tissues |
Genevisiblei | Q63943, MM |
Family and domain databases
CDDi | cd00265, MADS_MEF2_like, 1 hit |
Gene3Di | 3.40.1810.10, 1 hit |
InterProi | View protein in InterPro IPR022102, HJURP_C IPR033896, MADS_MEF2-like IPR002100, TF_MADSbox IPR036879, TF_MADSbox_sf |
Pfami | View protein in Pfam PF12347, HJURP_C, 1 hit PF00319, SRF-TF, 1 hit |
PRINTSi | PR00404, MADSDOMAIN |
SMARTi | View protein in SMART SM00432, MADS, 1 hit |
SUPFAMi | SSF55455, SSF55455, 1 hit |
PROSITEi | View protein in PROSITE PS00350, MADS_BOX_1, 1 hit PS50066, MADS_BOX_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MEF2D_MOUSE | |
Accessioni | Q63943Primary (citable) accession number: Q63943 Secondary accession number(s): E9QKS9, Q63944 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 166 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families