UniProtKB - Q63767 (BCAR1_RAT)
Breast cancer anti-estrogen resistance protein 1
Bcar1
Functioni
Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion (By similarity).
Implicated in induction of cell migration and cell branching (By similarity).
Involved in the BCAR3-mediated inhibition of TGFB signaling (By similarity).
By similarityGO - Molecular functioni
- protein domain specific binding Source: RGD
- protein kinase binding Source: RGD
- SH3 domain binding Source: UniProtKB-KW
GO - Biological processi
- actin cytoskeleton organization Source: RGD
- actin filament organization Source: UniProtKB
- actin filament reorganization Source: GO_Central
- antigen receptor-mediated signaling pathway Source: UniProtKB
- B cell receptor signaling pathway Source: UniProtKB
- cell adhesion Source: UniProtKB-KW
- cell chemotaxis Source: RGD
- cell migration Source: RGD
- cellular response to endothelin Source: RGD
- cellular response to hepatocyte growth factor stimulus Source: RGD
- cellular response to nitric oxide Source: RGD
- endothelin receptor signaling pathway Source: UniProtKB
- epidermal growth factor receptor signaling pathway Source: UniProtKB
- G protein-coupled receptor signaling pathway Source: UniProtKB
- hepatocyte growth factor receptor signaling pathway Source: RGD
- insulin receptor signaling pathway Source: UniProtKB
- integrin-mediated signaling pathway Source: UniProtKB
- negative regulation of substrate adhesion-dependent cell spreading Source: RGD
- neurotrophin TRK receptor signaling pathway Source: UniProtKB
- platelet-derived growth factor receptor signaling pathway Source: UniProtKB
- positive regulation of cell migration Source: UniProtKB
- positive regulation of endothelial cell migration Source: RGD
- transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
- vascular endothelial growth factor receptor signaling pathway Source: RGD
Keywordsi
Biological process | Cell adhesion |
Enzyme and pathway databases
Reactomei | R-RNO-186763, Downstream signal transduction R-RNO-372708, p130Cas linkage to MAPK signaling for integrins R-RNO-4420097, VEGFA-VEGFR2 Pathway R-RNO-8849471, PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases |
Names & Taxonomyi
Protein namesi | Recommended name: Breast cancer anti-estrogen resistance protein 1Alternative name(s): CRK-associated substrate p130cas |
Gene namesi | Name:Bcar1 Synonyms:Cas, Crkas |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2406, Bcar1 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- focal adhesion By similarity
- axon By similarity
Note: Unphosphorylated form localizes in the cytoplasm (By similarity). Localizes to focal adhesion sites following integrin engagement (By similarity).By similarity
Cytoskeleton
- actin cytoskeleton Source: RGD
Plasma Membrane
- plasma membrane Source: RGD
Other locations
- axon Source: UniProtKB-SubCell
- cytoplasm Source: UniProtKB
- focal adhesion Source: UniProtKB
- lamellipodium Source: RGD
- membrane Source: RGD
- ruffle Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cell projection, CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064856 | 1 – 968 | Breast cancer anti-estrogen resistance protein 1Add BLAST | 968 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 226 | Phosphotyrosine; by SRCBy similarity | 1 | |
Modified residuei | 237 | PhosphoserineBy similarity | 1 | |
Modified residuei | 332 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 347 | Phosphotyrosine; by ABL11 Publication | 1 | |
Modified residuei | 367 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 390 | PhosphoserineBy similarity | 1 | |
Modified residuei | 460 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 470 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 508 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 526 | PhosphoserineBy similarity | 1 | |
Modified residuei | 535 | PhosphoserineBy similarity | 1 | |
Modified residuei | 737 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q63767 |
PTM databases
iPTMneti | Q63767 |
PhosphoSitePlusi | Q63767 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Forms complexes in vivo with PTK2/FAK1, adapter protein CRKL and LYN kinase. Can heterodimerize with NEDD9.
Component of a complex comprised of SH2D3C, BCAR1/CAS, and CRK (By similarity). Within the complex, interacts with SH2D3C (via C-terminus), and CRK (By similarity).
Part of a complex comprised of PTPRA, BCAR1, BCAR3 (via SH2 domain) and SRC; the formation of the complex is dependent on intergrin mediated-tyrosine phosphorylation of PTPRA (By similarity).
Interacts with BCAR3 (via Ras-GEF domain); the interaction regulates adhesion-dependent serine phosphorylation (By similarity).
Interacts with SMAD2 and SMAD3 (By similarity).
Interacts with NPHP1 (By similarity).
Interacts with PTK2B/PYK2 (By similarity).
Interacts (via C-terminus) with SH2D3C/CHAT isoform 2 (via C-terminus) (By similarity).
Interacts with activated CSPG4.
Interacts with BMX, INPPL1/SHIP2 and PEAK1 (By similarity).
Part of a collagen stimulated complex involved in cell migration composed of CDC42, CRK, TNK2 and BCAR1/p130cas (By similarity).
Interacts with TNK2 via SH3 domains.
Interacts with PTK2B/PYK2 (By similarity).
By similarityBinary interactionsi
Q63767
With | #Exp. | IntAct |
---|---|---|
PTPN1 [P18031] from Homo sapiens. | 5 | EBI-1176801,EBI-968788 |
GO - Molecular functioni
- protein domain specific binding Source: RGD
- protein kinase binding Source: RGD
- SH3 domain binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 247449, 5 interactors |
CORUMi | Q63767 |
IntActi | Q63767, 7 interactors |
MINTi | Q63767 |
STRINGi | 10116.ENSRNOP00000039940 |
Structurei
Secondary structure
3D structure databases
BMRBi | Q63767 |
SMRi | Q63767 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q63767 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 97 – 159 | SH3PROSITE-ProRule annotationAdd BLAST | 63 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 164 – 277 | DisorderedSequence analysisAdd BLAST | 114 | |
Regioni | 213 – 514 | Substrate for kinasesAdd BLAST | 302 | |
Regioni | 393 – 416 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 503 – 544 | DisorderedSequence analysisAdd BLAST | 42 | |
Regioni | 705 – 756 | DisorderedSequence analysisAdd BLAST | 52 | |
Regioni | 844 – 894 | Divergent helix-loop-helix motifAdd BLAST | 51 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 733 – 741 | SH3-bindingSequence analysis | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 166 – 188 | Pro residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 199 – 257 | Polar residuesSequence analysisAdd BLAST | 59 | |
Compositional biasi | 523 – 543 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 717 – 751 | Polar residuesSequence analysisAdd BLAST | 35 |
Domaini
Sequence similaritiesi
Keywords - Domaini
SH3 domain, SH3-bindingPhylogenomic databases
eggNOGi | ENOG502QQHE, Eukaryota |
InParanoidi | Q63767 |
OrthoDBi | 1086228at2759 |
PhylomeDBi | Q63767 |
Family and domain databases
CDDi | cd12001, SH3_BCAR1, 1 hit |
Gene3Di | 1.20.120.830, 1 hit |
InterProi | View protein in InterPro IPR028848, BCAR1 IPR035745, BCAR1_SH3 IPR021901, CAS_C IPR037362, CAS_fam IPR014928, Serine_rich_dom IPR038319, Serine_rich_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
PANTHERi | PTHR10654, PTHR10654, 1 hit PTHR10654:SF15, PTHR10654:SF15, 1 hit |
Pfami | View protein in Pfam PF12026, CAS_C, 1 hit PF08824, Serine_rich, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKYLVSVGAG PARRAGGLED VSWGPRVSRR PQSYRAARHV NESLPRSAFR
60 70 80 90 100
VPAAHGASVT PSAALGSGLP ETQPEAVCRG TEKPRFAEGC KPAASRDKNV
110 120 130 140 150
LAKALYDNVA ESPDELSFRK GDIMTVLERD TQGLDGWWLC SLHGRQGIVP
160 170 180 190 200
GNRLKILVGM YDKKPAAPGP GPPATPPQPQ PSLPQGVHTP VPPASQYSPM
210 220 230 240 250
LPTAYQPQPD NVYLVPTPSK TQQGLYQAPG PNPQFQSPPA KQTSTFSKQT
260 270 280 290 300
PHHSFPSPAT DLYQVPPGPG SPAQDIYQVP PSAGTGHDIY QVPPSLDTRS
310 320 330 340 350
WEGTKPPAKV VVPTRVGQGY VYEASQAEQD EYDTPRHLLA PGSQDIYDVP
360 370 380 390 400
PVRGLLPNQY GQEVYDTPPM AVKGPNGRDP LLDVYDVPPS VEKGLPPSNH
410 420 430 440 450
HSVYDVPPSV SKDVPDGPLL REETYDVPPA FAKPKPFDPT RHPLILAAPP
460 470 480 490 500
PDSPPAEDVY DVPPPAPDLY DVPPGLRRPG PGTLYDVPRE RVLPPEVADG
510 520 530 540 550
SVIDDGVYAV PPPAEREAPT DGKRLSASST GSTRSSQSAS SLEVVVPGRE
560 570 580 590 600
PLELEVAVET LARLQQGVST TVAHLLDLVG SASGPGGWRS TSEPQEPPVQ
610 620 630 640 650
DLKAAVAAVH GAVHELLEFA RSAVSSATHT SDRTLHAKLS RQLQKMEDVY
660 670 680 690 700
QTLVVHGQVL DSGRGGPGFT LDDLDRLVAC SRAVPEDAKQ LASFLHGNAS
710 720 730 740 750
LLFRRTKAPG PGPEGSSSLH LNPTDKASSI QSRPLPSPPK FTSQDSPDGQ
760 770 780 790 800
YENSEGGWME DYDYVHLQGK EEFEKTQKEL LEKGNIVRQG KGQLELQQLK
810 820 830 840 850
QFERLEQEVS RPIDHDLANW TPAQPLVPGR TGGLGPSDRQ LLLFYLEQCE
860 870 880 890 900
ANLTTLTDAV DAFFTAVATN QPPKIFVAHS KFVILSAHKL VFIGDTLSRQ
910 920 930 940 950
AKAADVRSKV THYSNLLCDL LRGIVATTKA AALQYPSPSA AQDMVDRVKE
960
LGHSTQQFRR VLGQLAAA
The sequence of this isoform differs from the canonical sequence as follows:
5-98: Missing.
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3ZE30 | D3ZE30_RAT | Breast cancer anti-estrogen resista... | Bcar1 | 968 | Annotation score: | ||
F1LVA8 | F1LVA8_RAT | Breast cancer anti-estrogen resista... | Bcar1 | 968 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_004135 | 5 – 98 | Missing in isoform Short. CuratedAdd BLAST | 94 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D29766 mRNA Translation: BAA06169.1 D29766 mRNA Translation: BAA06170.1 |
PIRi | S46992 |
RefSeqi | NP_037063.1, NM_012931.1 [Q63767-1] |
Genome annotation databases
GeneIDi | 25414 |
KEGGi | rno:25414 |
UCSCi | RGD:2406, rat [Q63767-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D29766 mRNA Translation: BAA06169.1 D29766 mRNA Translation: BAA06170.1 |
PIRi | S46992 |
RefSeqi | NP_037063.1, NM_012931.1 [Q63767-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1X27 | X-ray | 2.70 | I/J/K/L/M/N | 759-767 | [»] | |
1Z23 | NMR | - | A | 546-708 | [»] | |
BMRBi | Q63767 | |||||
SMRi | Q63767 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 247449, 5 interactors |
CORUMi | Q63767 |
IntActi | Q63767, 7 interactors |
MINTi | Q63767 |
STRINGi | 10116.ENSRNOP00000039940 |
PTM databases
iPTMneti | Q63767 |
PhosphoSitePlusi | Q63767 |
Proteomic databases
PaxDbi | Q63767 |
Genome annotation databases
GeneIDi | 25414 |
KEGGi | rno:25414 |
UCSCi | RGD:2406, rat [Q63767-1] |
Organism-specific databases
CTDi | 9564 |
RGDi | 2406, Bcar1 |
Phylogenomic databases
eggNOGi | ENOG502QQHE, Eukaryota |
InParanoidi | Q63767 |
OrthoDBi | 1086228at2759 |
PhylomeDBi | Q63767 |
Enzyme and pathway databases
Reactomei | R-RNO-186763, Downstream signal transduction R-RNO-372708, p130Cas linkage to MAPK signaling for integrins R-RNO-4420097, VEGFA-VEGFR2 Pathway R-RNO-8849471, PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases |
Miscellaneous databases
EvolutionaryTracei | Q63767 |
PROi | PR:Q63767 |
Family and domain databases
CDDi | cd12001, SH3_BCAR1, 1 hit |
Gene3Di | 1.20.120.830, 1 hit |
InterProi | View protein in InterPro IPR028848, BCAR1 IPR035745, BCAR1_SH3 IPR021901, CAS_C IPR037362, CAS_fam IPR014928, Serine_rich_dom IPR038319, Serine_rich_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
PANTHERi | PTHR10654, PTHR10654, 1 hit PTHR10654:SF15, PTHR10654:SF15, 1 hit |
Pfami | View protein in Pfam PF12026, CAS_C, 1 hit PF08824, Serine_rich, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BCAR1_RAT | |
Accessioni | Q63767Primary (citable) accession number: Q63767 Secondary accession number(s): Q63766 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families