UniProtKB - Q63688 (CP7B1_RAT)
Protein
25-hydroxycholesterol 7-alpha-hydroxylase
Gene
Cyp7b1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Oxysterol 7alpha-hydroxylase that mediates formation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) from 25-hydroxycholesterol. Plays a key role in cell positioning and movement in lymphoid tissues: 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells.By similarity
Catalytic activityi
- 25-hydroxycholesterol + O2 + reduced [NADPH—hemoprotein reductase] = 7α,25-dihydroxycholesterol + H+ + H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationEC:1.14.14.291 Publication
- (25R)-cholest-5-ene-3β,26-diol + O2 + reduced [NADPH—hemoprotein reductase] = (25R)-cholest-5-en-3β,7α,26-triol + H+ + H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationEC:1.14.14.291 Publication
Cofactori
hemeBy similarity
: bile acid biosynthesis Pathwayi
This protein is involved in the pathway bile acid biosynthesis, which is part of Lipid metabolism.View all proteins of this organism that are known to be involved in the pathway bile acid biosynthesis and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 354 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- 25-hydroxycholesterol 7alpha-hydroxylase activity Source: UniProtKB-EC
- 27-hydroxycholesterol 7-alpha-monooxygenase activity Source: UniProtKB-EC
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- oxysterol 7-alpha-hydroxylase activity Source: UniProtKB
- steroid hydroxylase activity Source: GO_Central
GO - Biological processi
- B cell chemotaxis Source: UniProtKB
- bile acid biosynthetic process Source: RGD
- cholesterol homeostasis Source: GO_Central
- cholesterol metabolic process Source: RGD
- circadian rhythm Source: RGD
- memory Source: RGD
- negative regulation of intracellular estrogen receptor signaling pathway Source: RGD
- positive regulation of epithelial cell proliferation Source: RGD
- prostate gland epithelium morphogenesis Source: RGD
- response to cAMP Source: RGD
- response to drug Source: RGD
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00221 |
Names & Taxonomyi
Protein namesi | Recommended name: 25-hydroxycholesterol 7-alpha-hydroxylase (EC:1.14.14.291 Publication)Alternative name(s): Cytochrome P450 7B1 Hippocampal transcript 1 protein Short name: HCT-1 Oxysterol 7-alpha-hydroxylase |
Gene namesi | Name:Cyp7b1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2483, Cyp7b1 |
Subcellular locationi
Endoplasmic reticulum
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051908 | ‹1 – 414 | 25-hydroxycholesterol 7-alpha-hydroxylaseAdd BLAST | ›414 |
Proteomic databases
PaxDbi | Q63688 |
PRIDEi | Q63688 |
PTM databases
PhosphoSitePlusi | Q63688 |
Expressioni
Tissue specificityi
Highly expressed in brain; also expressed in liver and kidney.
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Phylogenomic databases
eggNOGi | KOG0684, Eukaryota |
InParanoidi | Q63688 |
OrthoDBi | 614788at2759 |
PhylomeDBi | Q63688 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR002403, Cyt_P450_E_grp-IV IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00465, EP450IV |
SUPFAMi | SSF48264, SSF48264, 1 hit |
(1+)i Sequence
Sequence statusi: Fragment.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q63688-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
ALEYQYVMKN PKQLSFEKFS RRLSAKAFSV KKLLTNDDLS NDIHRGYLLL
60 70 80 90 100
QGKSLDGLLE TMIQEVKEIF ESRLLKLTDW NTARVFDFCS SLVFEITFTT
110 120 130 140 150
IYGKILAANK KQIISELRDD FLKFDDHFPY LVSDIPIQLL RNAEFMQKKI
160 170 180 190 200
IKCLTPEKVA QMQRRSEIVQ ERQEMLKKYY GHEEFEIGAH HLGLLWASLA
210 220 230 240 250
NTIPAMFWAM YYLLQHPEAM EVLRDEIDSF LQSTGQKKGP GISVHFTREQ
260 270 280 290 300
LDSLVCLESA ILEVLRLCSY SSIIREVQED MDFSSESRSY RLRKGDFVAV
310 320 330 340 350
FPPMIHNDPE VFDAPKDFRF DRFVEDGKKK TTFFKGGKKL KSYIIPFGLG
360 370 380 390 400
TSKCPGRYFA INEMKLLVII LLTYFDLEVI DTKPIGLNHS RMFLGIQHPD
410
SDISFRYKAK SWRS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V787 | G3V787_RAT | 25-hydroxycholesterol 7-alpha-hydro... | Cyp7b1 rCG_41340 | 507 | Annotation score: | ||
A0A0G2K4N5 | A0A0G2K4N5_RAT | 25-hydroxycholesterol 7-alpha-hydro... | Cyp7b1 | 474 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U36992 mRNA Translation: AAA92616.1 |
RefSeqi | NP_062011.1, NM_019138.1 |
Genome annotation databases
GeneIDi | 25429 |
KEGGi | rno:25429 |
UCSCi | RGD:2483, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U36992 mRNA Translation: AAA92616.1 |
RefSeqi | NP_062011.1, NM_019138.1 |
3D structure databases
SMRi | Q63688 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000013116 |
PTM databases
PhosphoSitePlusi | Q63688 |
Proteomic databases
PaxDbi | Q63688 |
PRIDEi | Q63688 |
Genome annotation databases
GeneIDi | 25429 |
KEGGi | rno:25429 |
UCSCi | RGD:2483, rat |
Organism-specific databases
CTDi | 9420 |
RGDi | 2483, Cyp7b1 |
Phylogenomic databases
eggNOGi | KOG0684, Eukaryota |
InParanoidi | Q63688 |
OrthoDBi | 614788at2759 |
PhylomeDBi | Q63688 |
Enzyme and pathway databases
UniPathwayi | UPA00221 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR002403, Cyt_P450_E_grp-IV IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00465, EP450IV |
SUPFAMi | SSF48264, SSF48264, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CP7B1_RAT | |
Accessioni | Q63688Primary (citable) accession number: Q63688 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families