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Protein

Cyclin-dependent kinase 16

Gene

Cdk16

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Can phosphorylate CCNY at 'Ser-336' (in vitro) Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Regulates GH1 release by brain neurons. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Phosphorylates NSF, and thereby regulates NSF oligomerization.By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei194ATPPROSITE-ProRule annotation1
Active sitei286Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi171 – 179ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processDifferentiation, Spermatogenesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 16 (EC:2.7.11.22)
Alternative name(s):
Cell division protein kinase 16
PCTAIRE-motif protein kinase 1
Serine/threonine-protein kinase PCTAIRE-1
Gene namesi
Name:Cdk16
Synonyms:Pctaire1, Pctk1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620584 Cdk16

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoplasmic vesicle, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000864861 – 496Cyclin-dependent kinase 16Add BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12Phosphoserine; by BRSK2By similarity1
Modified residuei65PhosphoserineBy similarity1
Modified residuei78PhosphoserineCombined sources1
Modified residuei82PhosphoserineBy similarity1
Modified residuei95PhosphoserineCombined sources1
Modified residuei110PhosphoserineCombined sources1
Modified residuei119PhosphoserineBy similarity1
Modified residuei138PhosphoserineBy similarity1
Modified residuei153PhosphoserineBy similarity1
Modified residuei155PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation at Ser-153 inhibits kinase activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ63686
PRIDEiQ63686

PTM databases

iPTMnetiQ63686
PhosphoSitePlusiQ63686

Expressioni

Tissue specificityi

Detected in brain (at protein level). Isoform 1 and isoform 2 are expressed in brain, kidney, lung, heart and lens fiber.2 Publications

Interactioni

Subunit structurei

Interacts with BRSK2. Interacts with CCNY; this increases the CDK16 kinase activity (By similarity). Found in a complex containing CABLES1, CDK17 and TDRD7. Interacts with YWHAH, YWHAQ and YWHAZ. Interacts with NSF. Identified in a complex with NSF, syntaxin-1, synaptotagmin, SYN1, SYP and CDK5R1.By similarity2 Publications

Protein-protein interaction databases

IntActiQ63686, 1 interactor
STRINGi10116.ENSRNOP00000060222

Structurei

3D structure databases

ProteinModelPortaliQ63686
SMRiQ63686
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini165 – 446Protein kinasePROSITE-ProRule annotationAdd BLAST282

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0594 Eukaryota
ENOG410XPP3 LUCA
HOGENOMiHOG000233024
HOVERGENiHBG014652
InParanoidiQ63686
KOiK08820
PhylomeDBiQ63686

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q63686-1) [UniParc]FASTAAdd to basket
Also known as: PCTAIRE-1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDRMKKIKRQ LSMTLRGGRG IDKTNGVPEQ IGLDESGGGG GMTLGEAPTR
60 70 80 90 100
VAPGELRSIR GPLSSAPEIV HEDMKMGSDG ESDQASATSS DEVQSPVRVR
110 120 130 140 150
MRNHPPRKIS TEDINKCLSL PADIRLPEGY LEKLTLNSPI GDKPLSRRLR
160 170 180 190 200
PVSLSEIGFG KLETYIKLDK LGEGTYATVY KGKSKLTDNL VALKEIRLEH
210 220 230 240 250
EEGAPCTAIR EVSLLKDLKH ANIVTLHDII HTEKSLTLVF EYLDKDLKQY
260 270 280 290 300
LDDCGNVINM HNVKLFLFQL LRGLAYCHRQ KVLHRDLKPQ NLLINERGEL
310 320 330 340 350
KLADFGLAYA KSIPTKTYSN EVVTLWYRPP DILLGSTDYS GQIDMWGVGC
360 370 380 390 400
IFYEMATGRP LFPGSTVEEQ LHFIFRILGT PTEDTWPGIL SNEEFRTYNY
410 420 430 440 450
PKYRAEALLR HAPRLECDGA DLLTKLLQFE GRNRISAEDA MKHPFFLSLG
460 470 480 490
ERIHKLPDTT SIFALKEVQL QKEANIRSTS MPDSGRPAFR VVDTEF
Length:496
Mass (Da):55,765
Last modified:November 1, 1996 - v1
Checksum:i8E77DB7551A84329
GO
Isoform 2 (identifier: Q63686-2) [UniParc]FASTAAdd to basket
Also known as: PCTAIRE-1b

The sequence of this isoform differs from the canonical sequence as follows:
     1-64: MDRMKKIKRQ...ELRSIRGPLS → MYTNGYDEEIYYIGGKRVFLTPKAWPFPH

Show »
Length:461
Mass (Da):52,523
Checksum:iF543D3C9615AF987
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0106391 – 64MDRMK…RGPLS → MYTNGYDEEIYYIGGKRVFL TPKAWPFPH in isoform 2. 1 PublicationAdd BLAST64

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36444 mRNA Translation: AAC52912.1
U36444 mRNA Translation: AAC52913.1
PIRiJC5110
JC5111
RefSeqiNP_112339.1, NM_031077.1 [Q63686-2]
UniGeneiRn.16871

Genome annotation databases

GeneIDi81741
KEGGirno:81741
UCSCiRGD:620584 rat [Q63686-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCDK16_RAT
AccessioniPrimary (citable) accession number: Q63686
Secondary accession number(s): Q63687
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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