UniProtKB - Q63673 (SHH_RAT)
Sonic hedgehog protein
Shh
Functioni
Caution
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 90 | Calcium 1By similarity | 1 | |
Metal bindingi | 91 | Calcium 1By similarity | 1 | |
Metal bindingi | 91 | Calcium 2By similarity | 1 | |
Metal bindingi | 96 | Calcium 1By similarity | 1 | |
Metal bindingi | 126 | Calcium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 127 | Calcium 1By similarity | 1 | |
Metal bindingi | 127 | Calcium 2By similarity | 1 | |
Metal bindingi | 130 | Calcium 2By similarity | 1 | |
Metal bindingi | 132 | Calcium 2By similarity | 1 | |
Metal bindingi | 141 | ZincBy similarity | 1 | |
Metal bindingi | 148 | ZincBy similarity | 1 | |
Metal bindingi | 183 | ZincBy similarity | 1 | |
Sitei | 244 | Involved in cholesterol transferBy similarity | 1 | |
Sitei | 268 | Involved in auto-cleavageBy similarity | 1 | |
Sitei | 271 | Essential for auto-cleavageBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- glycosaminoglycan binding Source: RGD
- laminin-1 binding Source: UniProtKB
- patched binding Source: RGD
- zinc ion binding Source: UniProtKB
GO - Biological processi
- anatomical structure development Source: RGD
- anatomical structure formation involved in morphogenesis Source: RGD
- androgen metabolic process Source: UniProtKB
- angiogenesis Source: RGD
- animal organ formation Source: RGD
- anterior/posterior pattern specification Source: RGD
- apoptotic signaling pathway Source: UniProtKB
- artery development Source: RGD
- axon guidance Source: UniProtKB
- Bergmann glial cell differentiation Source: RGD
- blood coagulation Source: RGD
- branching involved in blood vessel morphogenesis Source: UniProtKB
- branching involved in prostate gland morphogenesis Source: RGD
- branching involved in salivary gland morphogenesis Source: RGD
- branching involved in ureteric bud morphogenesis Source: UniProtKB
- branching morphogenesis of an epithelial tube Source: UniProtKB
- bud outgrowth involved in lung branching Source: RGD
- camera-type eye development Source: RGD
- canonical Wnt signaling pathway Source: RGD
- CD4-positive or CD8-positive, alpha-beta T cell lineage commitment Source: UniProtKB
- cell-cell signaling Source: UniProtKB
- cell development Source: UniProtKB
- cell fate commitment Source: RGD
- cell fate specification Source: UniProtKB
- cell population proliferation Source: RGD
- cell proliferation in external granule layer Source: RGD
- cellular response to lithium ion Source: RGD
- central nervous system development Source: UniProtKB
- cerebellar granule cell precursor proliferation Source: UniProtKB
- commissural neuron axon guidance Source: RGD
- determination of left/right asymmetry in lateral mesoderm Source: RGD
- determination of left/right symmetry Source: RGD
- developmental growth Source: RGD
- digestive tract mesoderm development Source: RGD
- digestive tract morphogenesis Source: RGD
- dorsal/ventral neural tube patterning Source: RGD
- dorsal/ventral pattern formation Source: UniProtKB
- ectoderm development Source: RGD
- embryonic digestive tract morphogenesis Source: RGD
- embryonic digit morphogenesis Source: UniProtKB
- embryonic foregut morphogenesis Source: RGD
- embryonic forelimb morphogenesis Source: RGD
- embryonic hindlimb morphogenesis Source: RGD
- embryonic limb morphogenesis Source: UniProtKB
- embryonic morphogenesis Source: RGD
- embryonic organ development Source: RGD
- embryonic skeletal system development Source: RGD
- endocytosis Source: RGD
- epithelial cell proliferation involved in salivary gland morphogenesis Source: RGD
- epithelial-mesenchymal cell signaling Source: RGD
- epithelial-mesenchymal signaling involved in prostate gland development Source: RGD
- epithelial tube branching involved in lung morphogenesis Source: RGD
- establishment of cell polarity Source: RGD
- forebrain development Source: UniProtKB
- forebrain regionalization Source: RGD
- formation of anatomical boundary Source: RGD
- fungiform papilla development Source: RGD
- fungiform papilla formation Source: RGD
- fungiform papilla morphogenesis Source: RGD
- hair follicle development Source: RGD
- hair follicle morphogenesis Source: RGD
- heart development Source: UniProtKB
- heart looping Source: RGD
- hindbrain development Source: UniProtKB
- hindgut morphogenesis Source: RGD
- inner ear development Source: RGD
- intein-mediated protein splicing Source: InterPro
- intermediate filament organization Source: RGD
- kidney development Source: RGD
- left lung development Source: RGD
- limb bud formation Source: RGD
- limb development Source: RGD
- liver regeneration Source: RGD
- lung-associated mesenchyme development Source: RGD
- lung development Source: UniProtKB
- lung epithelium development Source: RGD
- lung lobe morphogenesis Source: RGD
- lung morphogenesis Source: RGD
- lymphoid progenitor cell differentiation Source: UniProtKB
- male genitalia development Source: UniProtKB
- male genitalia morphogenesis Source: RGD
- mesenchymal cell proliferation Source: RGD
- mesenchymal cell proliferation involved in lung development Source: RGD
- mesenchymal smoothened signaling pathway involved in prostate gland development Source: RGD
- metanephric collecting duct development Source: RGD
- metanephric mesenchymal cell proliferation involved in metanephros development Source: UniProtKB
- metanephros development Source: UniProtKB
- midbrain development Source: UniProtKB
- myoblast differentiation Source: RGD
- myotube differentiation Source: RGD
- negative regulation of alpha-beta T cell differentiation Source: RGD
- negative regulation of apoptotic process Source: RGD
- negative regulation of canonical Wnt signaling pathway Source: RGD
- negative regulation of cell differentiation Source: UniProtKB
- negative regulation of cell migration Source: UniProtKB
- negative regulation of cholesterol efflux Source: RGD
- negative regulation of dopaminergic neuron differentiation Source: RGD
- negative regulation of gene expression Source: RGD
- negative regulation of kidney smooth muscle cell differentiation Source: UniProtKB
- negative regulation of mesenchymal cell apoptotic process Source: RGD
- negative regulation of neuron death Source: RGD
- negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: RGD
- negative regulation of protein catabolic process Source: RGD
- negative regulation of T cell proliferation Source: RGD
- negative regulation of transcription by RNA polymerase II Source: RGD
- negative regulation of transcription elongation from RNA polymerase II promoter Source: RGD
- negative regulation of ureter smooth muscle cell differentiation Source: UniProtKB
- negative regulation of Wnt signaling pathway Source: RGD
- negative thymic T cell selection Source: UniProtKB
- neural crest cell migration Source: UniProtKB
- neural tube formation Source: RGD
- neuroblast proliferation Source: UniProtKB
- neuron fate commitment Source: UniProtKB
- odontogenesis Source: RGD
- odontogenesis of dentin-containing tooth Source: RGD
- oligodendrocyte development Source: RGD
- oligodendrocyte differentiation Source: RGD
- osteoblast development Source: RGD
- pancreas development Source: RGD
- pattern specification process Source: UniProtKB
- polarity specification of anterior/posterior axis Source: UniProtKB
- positive regulation of alpha-beta T cell differentiation Source: UniProtKB
- positive regulation of cell differentiation Source: RGD
- positive regulation of cell division Source: RGD
- positive regulation of cell population proliferation Source: RGD
- positive regulation of cerebellar granule cell precursor proliferation Source: RGD
- positive regulation of endothelial cell chemotaxis Source: RGD
- positive regulation of epithelial cell proliferation involved in prostate gland development Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of hh target transcription factor activity Source: RGD
- positive regulation of immature T cell proliferation in thymus Source: UniProtKB
- positive regulation of kidney smooth muscle cell differentiation Source: UniProtKB
- positive regulation of mesenchymal cell proliferation Source: RGD
- positive regulation of mesenchymal cell proliferation involved in ureter development Source: UniProtKB
- positive regulation of neuroblast proliferation Source: RGD
- positive regulation of neuron differentiation Source: RGD
- positive regulation of neurotrophin production Source: RGD
- positive regulation of oligodendrocyte differentiation Source: RGD
- positive regulation of oligodendrocyte progenitor proliferation Source: RGD
- positive regulation of penile erection Source: RGD
- positive regulation of photoreceptor cell differentiation Source: RGD
- positive regulation of protein import into nucleus Source: RGD
- positive regulation of sclerotome development Source: RGD
- positive regulation of skeletal muscle cell proliferation Source: RGD
- positive regulation of skeletal muscle tissue development Source: RGD
- positive regulation of smoothened signaling pathway Source: UniProtKB
- positive regulation of sprouting angiogenesis Source: RGD
- positive regulation of striated muscle cell differentiation Source: RGD
- positive regulation of T cell differentiation in thymus Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: RGD
- positive regulation of ureter smooth muscle cell differentiation Source: UniProtKB
- positive regulation of Wnt signaling pathway Source: RGD
- positive thymic T cell selection Source: UniProtKB
- primary prostatic bud elongation Source: RGD
- prostate epithelial cord elongation Source: RGD
- prostate gland development Source: RGD
- protein autoprocessing Source: InterPro
- protein localization to nucleus Source: RGD
- regulation of cell population proliferation Source: RGD
- regulation of epithelial cell differentiation Source: RGD
- regulation of epithelial cell proliferation involved in prostate gland development Source: RGD
- regulation of gene expression Source: RGD
- regulation of mesenchymal cell proliferation involved in prostate gland development Source: RGD
- regulation of odontogenesis Source: RGD
- regulation of prostatic bud formation Source: RGD
- regulation of protein localization to nucleus Source: RGD
- regulation of proteolysis Source: UniProtKB
- regulation of transcription, DNA-templated Source: RGD
- respiratory tube development Source: RGD
- response to axon injury Source: RGD
- response to estradiol Source: RGD
- response to ethanol Source: RGD
- response to hypoxia Source: RGD
- response to organonitrogen compound Source: RGD
- response to retinoic acid Source: RGD
- right lung development Source: RGD
- roof of mouth development Source: RGD
- salivary gland cavitation Source: RGD
- skin development Source: RGD
- smoothened signaling pathway Source: UniProtKB
- smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation Source: RGD
- smooth muscle tissue development Source: RGD
- somite development Source: RGD
- spinal cord dorsal/ventral patterning Source: MGI
- spinal cord motor neuron differentiation Source: RGD
- stem cell development Source: UniProtKB
- striated muscle cell differentiation Source: RGD
- striated muscle tissue development Source: RGD
- T cell differentiation in thymus Source: UniProtKB
- telencephalon regionalization Source: RGD
- thalamus development Source: RGD
- thymus development Source: UniProtKB
- thyroid gland development Source: RGD
- tongue development Source: RGD
- tongue morphogenesis Source: RGD
- trachea development Source: RGD
- trachea morphogenesis Source: RGD
- tracheoesophageal septum formation Source: RGD
- vasculature development Source: RGD
- vasculogenesis Source: UniProtKB
- ventral spinal cord interneuron specification Source: MGI
- zona limitans intrathalamica formation Source: RGD
Keywordsi
Molecular function | Developmental protein, Hydrolase, Protease |
Ligand | Calcium, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-5358346, Hedgehog ligand biogenesis R-RNO-5362798, Release of Hh-Np from the secreting cell R-RNO-5632681, Ligand-receptor interactions R-RNO-5635838, Activation of SMO |
Protein family/group databases
MEROPSi | C46.002 |
Names & Taxonomyi
Protein namesi | Recommended name: Sonic hedgehog proteinShort name: SHH Alternative name(s): Shh unprocessed N-terminal signaling and C-terminal autoprocessing domainsBy similarity Short name: ShhNCBy similarity Cleaved into the following chain: Alternative name(s): Shh N-terminal processed signaling domainsBy similarity Short name: ShhNpBy similarity |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3673, Shh |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Lipid-anchor By similarity
Note: The dual-lipidated sonic hedgehog protein N-product (ShhNp) is firmly tethered to the cell membrane where it forms multimers (By similarity). Further solubilization and release from the cell surface seem to be achieved through different mechanisms, including the interaction with DISP1 and SCUBE2, movement by lipoprotein particles, transport by cellular extensions called cytonemes or by the proteolytic removal of both terminal lipidated peptides.By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: RGD
Extracellular region or secreted
- extracellular matrix Source: RGD
- extracellular space Source: RGD
Golgi apparatus
- Golgi apparatus Source: RGD
Nucleus
- nucleus Source: RGD
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- axon Source: RGD
- cell surface Source: UniProtKB
- dendrite Source: RGD
- membrane Source: RGD
- membrane raft Source: RGD
- neuronal cell body Source: RGD
- synaptic vesicle Source: RGD
- transport vesicle Source: RGD
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | By similarityAdd BLAST | 24 | |
ChainiPRO_0000013214 | 25 – 437 | Sonic hedgehog proteinAdd BLAST | 413 | |
ChainiPRO_0000013215 | 25 – 198 | Sonic hedgehog protein N-productAdd BLAST | 174 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 25 | N-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 198 | Cholesterol glycine esterBy similarity | 1 | |
Glycosylationi | 279 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 198 – 199 | Cleavage; by autolysis | 2 |
Keywords - PTMi
Autocatalytic cleavage, Glycoprotein, Lipoprotein, PalmitateProteomic databases
PaxDbi | Q63673 |
PTM databases
GlyGeni | Q63673, 1 site |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Interacts with HHATL/GUP1 which negatively regulates HHAT-mediated palmitoylation of the SHH N-terminus (By similarity). ShhNp is active as a multimer (By similarity).
Interacts with BOC and CDON (By similarity).
Interacts with HHIP (By similarity).
Interacts with DISP1 via its cholesterol anchor (By similarity).
Interacts with SCUBE2 (By similarity).
Interacts with glypican GPC3 (By similarity).
By similarity1 PublicationGO - Molecular functioni
- laminin-1 binding Source: UniProtKB
- patched binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000008497 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 33 – 39 | Cardin-WeintraubBy similarity | 7 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 383 – 387 | Poly-Gly | 5 |
Domaini
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3638, Eukaryota |
InParanoidi | Q63673 |
OrthoDBi | 1169356at2759 |
PhylomeDBi | Q63673 |
Family and domain databases
Gene3Di | 3.30.1380.10, 1 hit |
InterProi | View protein in InterPro IPR001657, Hedgehog IPR001767, Hedgehog_Hint IPR009045, Hedgehog_sig/DD-Pept_Zn-bd_sf IPR000320, Hedgehog_signalling_dom IPR003586, Hint_dom_C IPR003587, Hint_dom_N IPR036844, Hint_dom_sf IPR006141, Intein_N |
Pfami | View protein in Pfam PF01085, HH_signal, 1 hit PF01079, Hint, 1 hit |
PIRSFi | PIRSF009400, Peptidase_C46, 1 hit |
PRINTSi | PR00632, SONICHHOG |
SMARTi | View protein in SMART SM00305, HintC, 1 hit SM00306, HintN, 1 hit |
SUPFAMi | SSF51294, SSF51294, 1 hit SSF55166, SSF55166, 1 hit |
PROSITEi | View protein in PROSITE PS50817, INTEIN_N_TER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MLLLLARCFL VALASSLLVC PGLACGPGRG FGKRQHPKKL TPLAYKQFIP
60 70 80 90 100
NVAEKTLGAS GRYEGKITRN SERFKELTPN YNPDIIFKDE ENTGADRLMT
110 120 130 140 150
QRCKDKLNAL AISVMNQWPG VKLRVTEGWD EDGHHSEESL HYEGRAVDIT
160 170 180 190 200
TSDRDRSKYG MLARLAVEAG FDWVYYESKA RIHCSVKAEN SVAAKSDGCF
210 220 230 240 250
PGSATVHLEQ GGTKLVKDLS PGDRVLAADD QGRLLYSDFL TFLDRDEGAK
260 270 280 290 300
KVFYVIETRE PRERLLLTAA HLLFVAPHND SGPTPGPSPL FASRVRPGQR
310 320 330 340 350
VYVVAERGGD RRLLPAAVHS VTLREEAAGA YAPLTADGTI LINRVLASCY
360 370 380 390 400
AVIEEHSWAH RAFAPFRLAH ALLAALAPAR TDGGGGGSIP APQSVAEARG
410 420 430
AGPPAGIHWY SQLLYHIGTW LLDSETLHPL GMAVKSS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V6T0 | G3V6T0_RAT | Hedgehog protein | Shh rCG_56752 | 437 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L27340 mRNA Translation: AAA20999.1 |
PIRi | B53193 |
RefSeqi | NP_058917.1, NM_017221.1 |
Genome annotation databases
GeneIDi | 29499 |
KEGGi | rno:29499 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L27340 mRNA Translation: AAA20999.1 |
PIRi | B53193 |
RefSeqi | NP_058917.1, NM_017221.1 |
3D structure databases
SMRi | Q63673 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000008497 |
Protein family/group databases
MEROPSi | C46.002 |
PTM databases
GlyGeni | Q63673, 1 site |
Proteomic databases
PaxDbi | Q63673 |
Genome annotation databases
GeneIDi | 29499 |
KEGGi | rno:29499 |
Organism-specific databases
CTDi | 6469 |
RGDi | 3673, Shh |
Phylogenomic databases
eggNOGi | KOG3638, Eukaryota |
InParanoidi | Q63673 |
OrthoDBi | 1169356at2759 |
PhylomeDBi | Q63673 |
Enzyme and pathway databases
Reactomei | R-RNO-5358346, Hedgehog ligand biogenesis R-RNO-5362798, Release of Hh-Np from the secreting cell R-RNO-5632681, Ligand-receptor interactions R-RNO-5635838, Activation of SMO |
Miscellaneous databases
PROi | PR:Q63673 |
Family and domain databases
Gene3Di | 3.30.1380.10, 1 hit |
InterProi | View protein in InterPro IPR001657, Hedgehog IPR001767, Hedgehog_Hint IPR009045, Hedgehog_sig/DD-Pept_Zn-bd_sf IPR000320, Hedgehog_signalling_dom IPR003586, Hint_dom_C IPR003587, Hint_dom_N IPR036844, Hint_dom_sf IPR006141, Intein_N |
Pfami | View protein in Pfam PF01085, HH_signal, 1 hit PF01079, Hint, 1 hit |
PIRSFi | PIRSF009400, Peptidase_C46, 1 hit |
PRINTSi | PR00632, SONICHHOG |
SMARTi | View protein in SMART SM00305, HintC, 1 hit SM00306, HintN, 1 hit |
SUPFAMi | SSF51294, SSF51294, 1 hit SSF55166, SSF55166, 1 hit |
PROSITEi | View protein in PROSITE PS50817, INTEIN_N_TER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SHH_RAT | |
Accessioni | Q63673Primary (citable) accession number: Q63673 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families