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Protein

Vesicle-associated membrane protein 1

Gene

Vamp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the targeting and/or fusion of transport vesicles to their target membrane.

Miscellaneous

Is not targeted by C.botulinum neurotoxin type B (BoNT/B) or by C.tetani toxin (tetX) as their target sequence is not conserved in this protein (PubMed:18511417, PubMed:8175689).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei60 – 61(Microbial infection) Cleavage; by C.botulinum neurotoxin type F (BoNT/F, botF)2 Publications2
Sitei61 – 62(Microbial infection) Cleavage; by C.botulinum neurotoxin type D (BoNT/D, botD)1 Publication2

GO - Molecular functioni

GO - Biological processi

  • exocytosis Source: GO_Central
  • intracellular protein transport Source: RGD
  • SNARE complex assembly Source: Ensembl
  • vesicle fusion Source: GO_Central
  • vesicle-mediated transport Source: RGD

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 1
Short name:
VAMP-1
Alternative name(s):
Synaptobrevin-1
Gene namesi
Name:Vamp1
Synonyms:Syb1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi3948 Vamp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 96CytoplasmicSequence analysisAdd BLAST96
Transmembranei97 – 116Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST20
Topological domaini117 – 118VesicularSequence analysis2

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Mitochondrion, Mitochondrion outer membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002067211 – 118Vesicle-associated membrane protein 1Add BLAST118

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei63PhosphoserineBy similarity1

Post-translational modificationi

(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which hydrolyzes the 61-Lys-|-Leu-62 bond and inhibits neurotransmitter release (PubMed:8175689). This is a poor substrate for BoNT/D, high concentrations are required to cleave it in vitro (PubMed:8175689).1 Publication
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which hydrolyzes the 60-Gln-|-Lys-61 bond and inhibits neurotransmitter release (PubMed:8505288).1 Publication2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ63666
PRIDEiQ63666

PTM databases

iPTMnetiQ63666
PhosphoSitePlusiQ63666
SwissPalmiQ63666

Expressioni

Tissue specificityi

Expressed in brain and spleen (at protein level). Isoform 1 expressed at very high level in brain. Even higher level found in spinal cord. Isoform 3 expressed in kidney, spleen and liver. Isoforms 2 and 3 expressed in osteoblasts of trabecular bone. Also expressed in heart.1 Publication

Gene expression databases

BgeeiENSRNOG00000019219
GenevisibleiQ63666 RN

Interactioni

Subunit structurei

Interacts with VAPA and VAPB.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi247653, 1 interactor
IntActiQ63666, 5 interactors
MINTiQ63666
STRINGi10116.ENSRNOP00000025081

Structurei

3D structure databases

ProteinModelPortaliQ63666
SMRiQ63666
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 93v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860 Eukaryota
COG5143 LUCA
GeneTreeiENSGT00550000074449
HOGENOMiHOG000042711
HOVERGENiHBG006675
InParanoidiQ63666
KOiK08510
OMAiMIMMGVI
OrthoDBiEOG091G0MPE
PhylomeDBiQ63666
TreeFamiTF313666

Family and domain databases

InterProiView protein in InterPro
IPR001388 Synaptobrevin
IPR016444 Synaptobrevin/VAMP
PfamiView protein in Pfam
PF00957 Synaptobrevin, 1 hit
PIRSFiPIRSF005409 Synaptobrevin_euk, 1 hit
PRINTSiPR00219 SYNAPTOBREVN
PROSITEiView protein in PROSITE
PS00417 SYNAPTOBREVIN, 1 hit
PS50892 V_SNARE, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q63666-1) [UniParc]FASTAAdd to basket
Also known as: Vamp1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAPAQPPAE GTEGAAPGGG PPGPPPNTTS NRRLQQTQAQ VEEVVDIMRV
60 70 80 90 100
NVDKVLERDQ KLSELDDRAD ALQAGASVFE SSAAKLKRKY WWKNCKMMIM
110
LGAICAIIVV VIVIYIFT
Length:118
Mass (Da):12,797
Last modified:November 13, 2007 - v2
Checksum:iE34D4D735CFBF6A8
GO
Isoform 2 (identifier: Q63666-2) [UniParc]FASTAAdd to basket
Also known as: Vamp1b

The sequence of this isoform differs from the canonical sequence as follows:
     114-118: IYIFT → SKYR

Show »
Length:117
Mass (Da):12,694
Checksum:iD0966B291BF6A8FC
GO
Isoform 3 (identifier: Q63666-3) [UniParc]FASTAAdd to basket
Also known as: Vamp1-ob

The sequence of this isoform differs from the canonical sequence as follows:
     114-118: IYIFT → RQD

Show »
Length:116
Mass (Da):12,558
Checksum:iA78EC8ABF6A8FCDC
GO
Isoform 4 (identifier: Q63666-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     114-118: IYIFT → NSGTEDRSCSVCFGSFC

Show »
Length:130
Mass (Da):13,940
Checksum:i9EFA0258B07BE3D8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48M → I in AAA42322 (PubMed:2472388).Curated1
Sequence conflicti49R → C in AAH92206 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_029188114 – 118IYIFT → SKYR in isoform 2. 2 Publications5
Alternative sequenceiVSP_029187114 – 118IYIFT → RQD in isoform 3. 2 Publications5
Alternative sequenceiVSP_029189114 – 118IYIFT → NSGTEDRSCSVCFGSFC in isoform 4. 1 Publication5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24104 mRNA Translation: AAA42322.1
U74621 mRNA Translation: AAC53427.1
AF498262 mRNA Translation: AAN85832.1
EF653274 mRNA Translation: ABR68027.1
EF653275 mRNA Translation: ABR68028.1
BC092206 mRNA Translation: AAH92206.1
PIRiA34288
RefSeqiNP_037222.2, NM_013090.2 [Q63666-1]
UniGeneiRn.31977

Genome annotation databases

EnsembliENSRNOT00000025081; ENSRNOP00000025081; ENSRNOG00000019219 [Q63666-1]
GeneIDi25624
KEGGirno:25624
UCSCiRGD:3948 rat [Q63666-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiVAMP1_RAT
AccessioniPrimary (citable) accession number: Q63666
Secondary accession number(s): A6YSN3
, O09025, Q56A22, Q8CH14
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 13, 2007
Last modified: July 18, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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