UniProtKB - Q63665 (USF2_RAT)

BLAST

>sp|Q63665|USF2_RAT Upstream stimulatory factor 2 OS=Rattus norvegicus OX=10116 GN=Usf2 PE=1 SV=2
MDMLDPGLDPASSATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAIASVQQAAFG
DHNIQYQFRTESNGGQVTYRVVQVTDGQLDGQGDAAGAVSVVSTAAFAGGQQAVTQVGVD
GAAQRPGPAAASVPTGPAAPFPLAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTA
VSVQTTDQSLQAGGQFYVMMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQH
NEVERRRRDKINNWIVQLSKIIPDCHADNSKTGASKGGILSKACDYIRELRQTNQRMQET
FKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGESTRQ

Align

Format
Add to basket Added to basket
History
Entry version 134 (07 Nov 2018)
Sequence version 2 (06 Dec 2005)
Previous versions | rss
Other tutorials and videos
Help video
Feedback

Protein

Upstream stimulatory factor 2

Gene

Usf2

Organism

Rattus norvegicus (Rat)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

GO - Molecular functionⁱ

DNA-binding transcription factor activity
Source: RGD
Inferred from mutant phenotypeⁱ
- 17408383
double-stranded DNA binding
Source: RGD
Inferred from direct assayⁱ
- 17408383
protein heterodimerization activity
Source: RGD
Inferred from mutant phenotypeⁱ
- 8576131
protein homodimerization activity
Source: RGD
Inferred from mutant phenotypeⁱ
- 8576131
RNA polymerase II proximal promoter sequence-specific DNA binding
Source: RGD
Inferred from direct assayⁱ
- 8576131
sequence-specific DNA binding
Source: RGD
Inferred from direct assayⁱ
- 17408383

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

positive regulation of transcription by RNA polymerase II
Source: RGD
Inferred from mutant phenotypeⁱ
- 17408383

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding
Biological process	Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Upstream stimulatory factor 2 Alternative name(s): Major late transcription factor 2 Upstream transcription factor 2
Gene namesⁱ	Name:Usf2
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Unplaced

Organism-specific databases

Rat genome database More...RGDⁱ	620975 Usf2

RGDⁱ

620975 Usf2

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000127502	1 – 346	Upstream stimulatory factor 2Add BLAST		346

Proteomic databases

PaxDbⁱ	Q63665

PaxDbⁱ

Q63665

PTM databases

iPTMnetⁱ	Q63665
PhosphoSitePlusⁱ	Q63665

Interactionⁱ

Subunit structureⁱ

Interacts with MAF (By similarity). Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2).By similarity

GO - Molecular functionⁱ

protein heterodimerization activity
Source: RGD
Inferred from mutant phenotypeⁱ
- 8576131
protein homodimerization activity
Source: RGD
Inferred from mutant phenotypeⁱ
- 8576131

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	10116.ENSRNOP00000028550

STRINGⁱ

10116.ENSRNOP00000028550

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q63665
SMRⁱ	Q63665
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	235 – 290	bHLHPROSITE-ProRule annotationAdd BLAST		56

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	307 – 328	Leucine-zipperAdd BLAST		22

Phylogenomic databases

eggNOGⁱ	ENOG410INSC Eukaryota ENOG4111DV1 LUCA
HOGENOMⁱ	HOG000294174
HOVERGENⁱ	HBG004346
InParanoidⁱ	Q63665
KEGG Orthology (KO) More...KOⁱ	K09106
PhylomeDBⁱ	Q63665
TreeFamⁱ	TF323338

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR024097 bHLH_ZIP_TF IPR036638 HLH_DNA-bd_sf
PANTHERⁱ	PTHR12565 PTHR12565, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit

Sequences (5+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 5 isoformsⁱ produced by alternative splicing. Align Add to basket

Note: At least 2 isoforms are produced.

This entry has 5 described isoforms and 1 potential isoform that is computationally mapped.Show all Align All

Isoform 1 (identifier: Q63665-1) [UniParc]FASTA Add to basket

Also known as: USF2L, USF2A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDMLDPGLDP ASSATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA 
        60         70         80         90        100
IASVQQAAFG DHNIQYQFRT ESNGGQVTYR VVQVTDGQLD GQGDAAGAVS 
       110        120        130        140        150
VVSTAAFAGG QQAVTQVGVD GAAQRPGPAA ASVPTGPAAP FPLAVIQNPF 
       160        170        180        190        200
SNGGSPAAEA VSGEARFAYF PASSVGDTTA VSVQTTDQSL QAGGQFYVMM 
       210        220        230        240        250
TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH NEVERRRRDK 
       260        270        280        290        300
INNWIVQLSK IIPDCHADNS KTGASKGGIL SKACDYIREL RQTNQRMQET 
       310        320        330        340 
FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GESTRQ

Length:346

Mass (Da):36,954

Last modified:December 6, 2005 - v2

Checksum:ⁱE9D216BC25F9447B

Isoform 2 (identifier: Q63665-2) [UniParc]FASTA Add to basket

Also known as: USF2delta1

The sequence of this isoform differs from the canonical sequence as follows:
117-143: Missing.

« Hide

        10         20         30         40         50
MDMLDPGLDP ASSATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA 
        60         70         80         90        100
IASVQQAAFG DHNIQYQFRT ESNGGQVTYR VVQVTDGQLD GQGDAAGAVS 
       110        120        130        140        150
VVSTAAFAGG QQAVTQAVIQ NPFSNGGSPA AEAVSGEARF AYFPASSVGD 
       160        170        180        190        200
TTAVSVQTTD QSLQAGGQFY VMMTPQDVLQ TGTQRTIAPR THPYSPKIDG 
       210        220        230        240        250
TRTPRDERRR AQHNEVERRR RDKINNWIVQ LSKIIPDCHA DNSKTGASKG 
       260        270        280        290        300
GILSKACDYI RELRQTNQRM QETFKEAERL QMDNELLRQQ IEELKNENAL 
       310 
LRAQLQQHNL EMVGESTRQ

Show »

Length:319

Mass (Da):34,500

Checksum:ⁱ5A5D8BC8007BB90F

Isoform 3 (identifier: Q63665-3) [UniParc]FASTA Add to basket

Also known as: USF2delta2, USF2B

The sequence of this isoform differs from the canonical sequence as follows:
77-143: Missing.

« Hide

        10         20         30         40         50
MDMLDPGLDP ASSATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA 
        60         70         80         90        100
IASVQQAAFG DHNIQYQFRT ESNGGQAVIQ NPFSNGGSPA AEAVSGEARF 
       110        120        130        140        150
AYFPASSVGD TTAVSVQTTD QSLQAGGQFY VMMTPQDVLQ TGTQRTIAPR 
       160        170        180        190        200
THPYSPKIDG TRTPRDERRR AQHNEVERRR RDKINNWIVQ LSKIIPDCHA 
       210        220        230        240        250
DNSKTGASKG GILSKACDYI RELRQTNQRM QETFKEAERL QMDNELLRQQ 
       260        270 
IEELKNENAL LRAQLQQHNL EMVGESTRQ

Show »

Length:279

Mass (Da):30,595

Checksum:ⁱD9474CE884D8A60B

Isoform 4 (identifier: Q63665-4) [UniParc]FASTA Add to basket

Also known as: USF2delta3

The sequence of this isoform differs from the canonical sequence as follows:
21-27: SHDKGPE → RRGRPGG
28-346: Missing.

« Hide

        10         20 
MDMLDPGLDP ASSATAAAAA RRGRPGG

Show »

Length:27

Mass (Da):2,613

Checksum:ⁱB5C9C83F09C71D5A

Isoform 5 (identifier: Q63665-5) [UniParc]FASTA Add to basket

Also known as: USF2delta4

The sequence of this isoform differs from the canonical sequence as follows:
21-22: SH → RL
23-346: Missing.

« Hide

        10         20 
MDMLDPGLDP ASSATAAAAA RL

Show »

Length:22

Mass (Da):2,145

Checksum:ⁱ243D5A861D66A6DA

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A0G2K3J3	A0A0G2K3J3_RAT		Upstream stimulatory factor 2 Upstream stimulatory factor 2	Usf2	339	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	95	A → T in CAA62338 (PubMed:8576131).Curated		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_016542	21 – 27	SHDKGPE → RRGRPGG in isoform 4. 1 Publication	7
Alternative sequenceⁱVSP_016543	21 – 22	SH → RL in isoform 5. 1 Publication	2
Alternative sequenceⁱVSP_016544	23 – 346	Missing in isoform 5. 1 PublicationAdd BLAST	324
Alternative sequenceⁱVSP_016545	28 – 346	Missing in isoform 4. 1 PublicationAdd BLAST	319
Alternative sequenceⁱVSP_016546	77 – 143	Missing in isoform 3. 1 PublicationAdd BLAST	67
Alternative sequenceⁱVSP_016547	117 – 143	Missing in isoform 2. 1 PublicationAdd BLAST	27

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB035647 mRNA Translation: BAB19964.1 AB035648 mRNA Translation: BAB19965.1 AB035649 mRNA Translation: BAB19966.1 AB035650 mRNA Translation: BAB19967.1 AB035651 mRNA Translation: BAB19968.1 AB047556 mRNA Translation: BAB20993.1 X90823 mRNA Translation: CAA62338.1
NCBI Reference Sequences More...RefSeqⁱ	NP_112401.1, NM_031139.1 [Q63665-1]
UniGeneⁱ	Rn.44637

Genome annotation databases

GeneIDⁱ	81817
KEGGⁱ	rno:81817
UCSC genome browser More...UCSCⁱ	RGD:620975 rat [Q63665-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q63665	Upstream stimulatory factor 2		MOUSE		346	UniRef100_Q64705
Q63665-2	Upstream stimulatory factor 2		MOUSE		319	UniRef100_F8WI12

Protein	Similar proteins		Species	Score	Length	Source
Q63665	Upstream stimulatory factor 2		HUMAN		346	UniRef90_Q15853
Upstream stimulatory factor 2		MOUSE		346
USF2 isoform 2		PANTR		346
USF2 isoform 2		PONAB		346
Uncharacterized protein		CAVPO		360
+61
Q63665-2	Upstream stimulatory factor 2		MOUSE		319	UniRef90_F8WI12
upstream stimulatory factor 2 isoform X2		PHYCD		320
UPI000A2B3D74		PIG		360
UPI0008479876		CAPHI		319
UPI00098B6086		MICMU		319
+8
Q63665-3	Isoform USF2B of Upstream stimulatory factor 2		HUMAN		279	UniRef90_Q15853-3
USF2 isoform 1		PANTR		279
USF2 isoform 1		PONAB		279
Upstream transcription factor 2, c-fos interacting		GORGO		258
upstream stimulatory factor 2 isoform X2		LIPVE		280
+26

Protein	Similar proteins		Species	Score	Length	Source
Q63665	Upstream stimulatory factor 2		HUMAN		346	UniRef50_Q15853
Upstream stimulatory factor 2		MOUSE		346
USF2 isoform 2		PANTR		346
USF2 isoform 2		PONAB		346
Uncharacterized protein		CAVPO		360
+97
Q63665-2	Upstream transcription factor 2, c-fos interacting		SHEEP		340	UniRef50_W5P3T0
USF2 (Fragment)		RABIT		277
upstream stimulatory factor 2		TARSY		275
Uncharacterized protein (Fragment)		MYOLU		271
Upstream stimulatory factor 2 (Fragment)		wild yak		329
+167
Q63665-3	Isoform USF2B of Upstream stimulatory factor 2		HUMAN		279	UniRef50_Q15853-3
USF2 isoform 1		PANTR		279
USF2 isoform 1		PONAB		279
Upstream transcription factor 2, c-fos interacting		GORGO		258
upstream stimulatory factor 2 isoform X2		LIPVE		280
+48
Q63665-4	Isoform 5 of Upstream stimulatory factor 2		RAT		22	UniRef50_Q63665-4
Uncharacterized protein		ARUDO		23
Succinyl-diaminopimelate desuccinylase (Fragment)		Flavobacteriaceae bacterium		22
Q63665-5	Isoform 4 of Upstream stimulatory factor 2		RAT		27	UniRef50_Q63665-4
Uncharacterized protein		ARUDO		23
Succinyl-diaminopimelate desuccinylase (Fragment)		Flavobacteriaceae bacterium		22

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB035647 mRNA Translation: BAB19964.1 AB035648 mRNA Translation: BAB19965.1 AB035649 mRNA Translation: BAB19966.1 AB035650 mRNA Translation: BAB19967.1 AB035651 mRNA Translation: BAB19968.1 AB047556 mRNA Translation: BAB20993.1 X90823 mRNA Translation: CAA62338.1
RefSeqⁱ	NP_112401.1, NM_031139.1 [Q63665-1]
UniGeneⁱ	Rn.44637

3D structure databases

ProteinModelPortalⁱ	Q63665
SMRⁱ	Q63665
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10116.ENSRNOP00000028550

STRINGⁱ

10116.ENSRNOP00000028550

PTM databases

iPTMnetⁱ	Q63665
PhosphoSitePlusⁱ	Q63665

Proteomic databases

PaxDbⁱ	Q63665

PaxDbⁱ

Q63665

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	81817
KEGGⁱ	rno:81817
UCSCⁱ	RGD:620975 rat [Q63665-1]

Organism-specific databases

CTDⁱ	7392
RGDⁱ	620975 Usf2

Phylogenomic databases

eggNOGⁱ	ENOG410INSC Eukaryota ENOG4111DV1 LUCA
HOGENOMⁱ	HOG000294174
HOVERGENⁱ	HBG004346
InParanoidⁱ	Q63665
KOⁱ	K09106
PhylomeDBⁱ	Q63665
TreeFamⁱ	TF323338

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q63665

PROⁱ

PR:Q63665

Family and domain databases

CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR024097 bHLH_ZIP_TF IPR036638 HLH_DNA-bd_sf
PANTHERⁱ	PTHR12565 PTHR12565, 1 hit
Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	USF2_RAT
Accessionⁱ	Q63665Primary (citable) accession number: Q63665 Secondary accession number(s): Q76MJ1 , Q76MU2, Q9EQX1, Q9EQX2, Q9EQX3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	December 6, 2005
	Last modified:	November 7, 2018
	This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q63665 (USF2_RAT)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Upstream stimulatory factor 2

Usf2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ