UniProtKB - Q63665 (USF2_RAT)
Upstream stimulatory factor 2
Usf2
Functioni
Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
GO - Molecular functioni
- bHLH transcription factor binding Source: RGD
- DNA binding Source: RGD
- DNA-binding transcription factor activity Source: RGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: RGD
- double-stranded DNA binding Source: RGD
- identical protein binding Source: RGD
- protein-containing complex binding Source: RGD
- protein heterodimerization activity Source: RGD
- protein homodimerization activity Source: RGD
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: RGD
- sequence-specific DNA binding Source: RGD
- sequence-specific double-stranded DNA binding Source: RGD
GO - Biological processi
- lactation Source: RGD
- lipid homeostasis Source: RGD
- positive regulation of transcription, DNA-templated Source: RGD
- positive regulation of transcription by RNA polymerase II Source: RGD
- positive regulation of transcription from RNA polymerase II promoter by glucose Source: RGD
- regulation of transcription by RNA polymerase II Source: RGD
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Upstream stimulatory factor 2Alternative name(s): Major late transcription factor 2 Upstream transcription factor 2 |
Gene namesi | Name:Usf2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620975, Usf2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127502 | 1 – 346 | Upstream stimulatory factor 2Add BLAST | 346 |
Proteomic databases
PaxDbi | Q63665 |
PTM databases
iPTMneti | Q63665 |
PhosphoSitePlusi | Q63665 |
Interactioni
Subunit structurei
Interacts with MAF (By similarity). Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2).
By similarityGO - Molecular functioni
- bHLH transcription factor binding Source: RGD
- identical protein binding Source: RGD
- protein heterodimerization activity Source: RGD
- protein homodimerization activity Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000028550 |
Structurei
3D structure databases
AlphaFoldDBi | Q63665 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 235 – 290 | bHLHPROSITE-ProRule annotationAdd BLAST | 56 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 44 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 215 – 244 | DisorderedSequence analysisAdd BLAST | 30 | |
Regioni | 307 – 328 | Leucine-zipperAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 226 – 244 | Basic and acidic residuesSequence analysisAdd BLAST | 19 |
Phylogenomic databases
eggNOGi | KOG1318, Eukaryota |
InParanoidi | Q63665 |
OrthoDBi | 1345445at2759 |
PhylomeDBi | Q63665 |
TreeFami | TF323338 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDMLDPGLDP ASSATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA
60 70 80 90 100
IASVQQAAFG DHNIQYQFRT ESNGGQVTYR VVQVTDGQLD GQGDAAGAVS
110 120 130 140 150
VVSTAAFAGG QQAVTQVGVD GAAQRPGPAA ASVPTGPAAP FPLAVIQNPF
160 170 180 190 200
SNGGSPAAEA VSGEARFAYF PASSVGDTTA VSVQTTDQSL QAGGQFYVMM
210 220 230 240 250
TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH NEVERRRRDK
260 270 280 290 300
INNWIVQLSK IIPDCHADNS KTGASKGGIL SKACDYIREL RQTNQRMQET
310 320 330 340
FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GESTRQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K3J3 | A0A0G2K3J3_RAT | Upstream stimulatory factor 2 | Usf2 | 348 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 95 | A → T in CAA62338 (PubMed:8576131).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016542 | 21 – 27 | SHDKGPE → RRGRPGG in isoform 4. 1 Publication | 7 | |
Alternative sequenceiVSP_016543 | 21 – 22 | SH → RL in isoform 5. 1 Publication | 2 | |
Alternative sequenceiVSP_016544 | 23 – 346 | Missing in isoform 5. 1 PublicationAdd BLAST | 324 | |
Alternative sequenceiVSP_016545 | 28 – 346 | Missing in isoform 4. 1 PublicationAdd BLAST | 319 | |
Alternative sequenceiVSP_016546 | 77 – 143 | Missing in isoform 3. 1 PublicationAdd BLAST | 67 | |
Alternative sequenceiVSP_016547 | 117 – 143 | Missing in isoform 2. 1 PublicationAdd BLAST | 27 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB035647 mRNA Translation: BAB19964.1 AB035648 mRNA Translation: BAB19965.1 AB035649 mRNA Translation: BAB19966.1 AB035650 mRNA Translation: BAB19967.1 AB035651 mRNA Translation: BAB19968.1 AB047556 mRNA Translation: BAB20993.1 X90823 mRNA Translation: CAA62338.1 |
RefSeqi | NP_112401.1, NM_031139.1 [Q63665-1] |
Genome annotation databases
Ensembli | ENSRNOT00000103509; ENSRNOP00000094253; ENSRNOG00000053725 [Q63665-3] |
GeneIDi | 81817 |
KEGGi | rno:81817 |
UCSCi | RGD:620975, rat [Q63665-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB035647 mRNA Translation: BAB19964.1 AB035648 mRNA Translation: BAB19965.1 AB035649 mRNA Translation: BAB19966.1 AB035650 mRNA Translation: BAB19967.1 AB035651 mRNA Translation: BAB19968.1 AB047556 mRNA Translation: BAB20993.1 X90823 mRNA Translation: CAA62338.1 |
RefSeqi | NP_112401.1, NM_031139.1 [Q63665-1] |
3D structure databases
AlphaFoldDBi | Q63665 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000028550 |
PTM databases
iPTMneti | Q63665 |
PhosphoSitePlusi | Q63665 |
Proteomic databases
PaxDbi | Q63665 |
Genome annotation databases
Ensembli | ENSRNOT00000103509; ENSRNOP00000094253; ENSRNOG00000053725 [Q63665-3] |
GeneIDi | 81817 |
KEGGi | rno:81817 |
UCSCi | RGD:620975, rat [Q63665-1] |
Organism-specific databases
CTDi | 7392 |
RGDi | 620975, Usf2 |
Phylogenomic databases
eggNOGi | KOG1318, Eukaryota |
InParanoidi | Q63665 |
OrthoDBi | 1345445at2759 |
PhylomeDBi | Q63665 |
TreeFami | TF323338 |
Miscellaneous databases
PROi | PR:Q63665 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | USF2_RAT | |
Accessioni | Q63665Primary (citable) accession number: Q63665 Secondary accession number(s): Q76MJ1 Q9EQX3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | December 6, 2005 | |
Last modified: | May 25, 2022 | |
This is version 147 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |