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Protein

Rhodopsin kinase

Gene

Grk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.By similarity

Catalytic activityi

ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei219ATPPROSITE-ProRule annotation1
Active sitei317Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi196 – 204ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • G-protein coupled receptor kinase activity Source: InterPro
  • rhodopsin kinase activity Source: RGD

GO - Biological processi

  • protein autophosphorylation Source: RGD
  • regulation of rhodopsin mediated signaling pathway Source: UniProtKB
  • response to drug Source: RGD
  • response to light stimulus Source: RGD
  • rhodopsin mediated signaling pathway Source: RGD
  • visual perception Source: UniProtKB-KW

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processSensory transduction, Vision
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.14 5301

Names & Taxonomyi

Protein namesi
Recommended name:
Rhodopsin kinase (EC:2.7.11.14By similarity)
Short name:
RK
Alternative name(s):
G protein-coupled receptor kinase 1Imported
Gene namesi
Name:Grk1Imported
Synonyms:Rhok
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619712 Grk1

Subcellular locationi

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000243791 – 561Rhodopsin kinaseAdd BLAST561
PropeptideiPRO_0000024380562 – 564Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5PhosphoserineBy similarity1
Modified residuei8PhosphothreonineBy similarity1
Modified residuei21Phosphoserine; by PKA and autocatalysis1 Publication1
Modified residuei491Phosphoserine; by autocatalysisBy similarity1
Modified residuei492Phosphothreonine; by autocatalysisBy similarity1
Modified residuei561Cysteine methyl esterBy similarity1
Lipidationi561S-farnesyl cysteineBy similarity1

Post-translational modificationi

Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.By similarity1 Publication
Farnesylation is required for full activity.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiQ63651
PRIDEiQ63651

PTM databases

iPTMnetiQ63651
PhosphoSitePlusiQ63651

Expressioni

Tissue specificityi

Detected in retina (at protein level) (PubMed:21504899). Retina-specific. Expressed in rod and cone photoreceptor cells.2 Publications

Interactioni

Subunit structurei

Interacts (when prenylated) with PDE6D; this promotes release from membranes.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024999

Structurei

3D structure databases

ProteinModelPortaliQ63651
SMRiQ63651
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 175RGSPROSITE-ProRule annotationAdd BLAST118
Domaini190 – 455Protein kinasePROSITE-ProRule annotationAdd BLAST266
Domaini456 – 521AGC-kinase C-terminalAdd BLAST66

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 189N-terminalAdd BLAST189
Regioni456 – 564C-terminalAdd BLAST109

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0986 Eukaryota
ENOG410YRQZ LUCA
HOGENOMiHOG000006742
HOVERGENiHBG004532
InParanoidiQ63651
KOiK00909
PhylomeDBiQ63651

Family and domain databases

CDDicd05608 STKc_GRK1, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR000239 GPCR_kinase
IPR032965 GRK1
IPR037716 GRK1_dom
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR016137 RGS
IPR036305 RGS_sf
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR24355:SF11 PTHR24355:SF11, 1 hit
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PF00615 RGS, 1 hit
PRINTSiPR00717 GPCRKINASE
SMARTiView protein in SMART
SM00315 RGS, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF48097 SSF48097, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50132 RGS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63651-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFGSLETVV ANSAFIAARG SFDGSSTPSS RDKKYLAKLR LPPLSKCEGL
60 70 80 90 100
RDSISLEFDN LCSEQPIGKR LFQQFLKTDE RHVPALELWK DIEDYDTADD
110 120 130 140 150
DLRPQKAQAI LAEYLDPQGT LFCNFLDQGM VARVKEGPTG SQDGLFQPLL
160 170 180 190 200
QATLEHLSQG PFQEYLGSLY FLRFLQWKWL EAQPIGEDWF LDFRVLGKGG
210 220 230 240 250
FGEVSACQMK ATGKMYACKK LNKKRLKKRK GYQGAIVEKR ILAKVHSRFI
260 270 280 290 300
VSLAYAFETK TDLCLVMTIM NGGDVRYHIY NVDEENPGFP EPRAIYYTAQ
310 320 330 340 350
IISGLEHLHQ RRIVYRDLKP ENVLLDNDGN IRISDLGLAV ELKEGQNKTK
360 370 380 390 400
GYAGTPGFMA PELLRGEEYD FSVDYFALGV TLYEMIAARG PFRARGEKVE
410 420 430 440 450
NKELKQRIIS EPVKYPEKFS QASKDFCEQL LEKDPEKRLG FRDGTCDALR
460 470 480 490 500
ANVLFKDISW RQLEAGMLIP PFIPDSRTVY AKNIQDVGAF STVKGVVFDK
510 520 530 540 550
ADTEFFQEFA SGNCSIPWQE EMIETGFFGD LNVWRPDGQM PDDMKGITVE
560
EAAPTAKSGM CLIS
Length:564
Mass (Da):63,769
Last modified:November 1, 1997 - v1
Checksum:i17E05784E6D1ED00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U63971 mRNA Translation: AAB05930.1
RefSeqiNP_112358.1, NM_031096.1
UniGeneiRn.10548

Genome annotation databases

GeneIDi81760
KEGGirno:81760
UCSCiRGD:619712 rat

Similar proteinsi

Entry informationi

Entry nameiRK_RAT
AccessioniPrimary (citable) accession number: Q63651
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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