UniProtKB - Q63569 (PRS6A_RAT)
Protein
26S proteasome regulatory subunit 6A
Gene
Psmc3
Organism
Rattus norvegicus (Rat)
Status
Functioni
Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC3 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 227 – 234 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATPase activity Source: InterPro
- ATP binding Source: UniProtKB-KW
- identical protein binding Source: RGD
GO - Biological processi
- blastocyst development Source: RGD
- modulation by host of viral transcription Source: RGD
- positive regulation of RNA polymerase II transcription preinitiation complex assembly Source: GO_Central
- positive regulation of transcription by RNA polymerase II Source: RGD
- protein catabolic process Source: InterPro
Keywordsi
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-1169091, Activation of NF-kappaB in B cells R-RNO-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-RNO-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-RNO-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-RNO-174154, APC/C:Cdc20 mediated degradation of Securin R-RNO-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-RNO-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-RNO-187577, SCF(Skp2)-mediated degradation of p27/p21 R-RNO-195253, Degradation of beta-catenin by the destruction complex R-RNO-202424, Downstream TCR signaling R-RNO-2467813, Separation of Sister Chromatids R-RNO-2871837, FCERI mediated NF-kB activation R-RNO-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-RNO-350562, Regulation of ornithine decarboxylase (ODC) R-RNO-382556, ABC-family proteins mediated transport R-RNO-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-RNO-4608870, Asymmetric localization of PCP proteins R-RNO-4641257, Degradation of AXIN R-RNO-4641258, Degradation of DVL R-RNO-5358346, Hedgehog ligand biogenesis R-RNO-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-RNO-5607764, CLEC7A (Dectin-1) signaling R-RNO-5610780, Degradation of GLI1 by the proteasome R-RNO-5610785, GLI3 is processed to GLI3R by the proteasome R-RNO-5632684, Hedgehog 'on' state R-RNO-5658442, Regulation of RAS by GAPs R-RNO-5668541, TNFR2 non-canonical NF-kB pathway R-RNO-5676590, NIK-->noncanonical NF-kB signaling R-RNO-5687128, MAPK6/MAPK4 signaling R-RNO-5689603, UCH proteinases R-RNO-5689880, Ub-specific processing proteases R-RNO-6798695, Neutrophil degranulation R-RNO-68827, CDT1 association with the CDC6:ORC:origin complex R-RNO-68949, Orc1 removal from chromatin R-RNO-69017, CDK-mediated phosphorylation and removal of Cdc6 R-RNO-69481, G2/M Checkpoints R-RNO-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-RNO-75815, Ubiquitin-dependent degradation of Cyclin D R-RNO-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-RNO-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-RNO-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-RNO-8939902, Regulation of RUNX2 expression and activity R-RNO-8941858, Regulation of RUNX3 expression and activity R-RNO-8948751, Regulation of PTEN stability and activity R-RNO-8951664, Neddylation R-RNO-9020702, Interleukin-1 signaling R-RNO-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Names & Taxonomyi
Protein namesi | Recommended name: 26S proteasome regulatory subunit 6AAlternative name(s): 26S proteasome AAA-ATPase subunit RPT5 Proteasome 26S subunit ATPase 3 Spermatogenic cell/sperm-associated Tat-binding protein homolog SATA Tat-binding protein 1 Short name: TBP-1 |
Gene namesi | Name:Psmc3 Synonyms:Tbp1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61905, Psmc3 |
Subcellular locationi
Nucleus
- nucleus Source: RGD
Other locations
- cytoplasm Source: RGD
- P-body Source: UniProtKB
- perinuclear region of cytoplasm Source: RGD
- proteasome accessory complex Source: UniProtKB
- proteasome complex Source: RGD
- proteasome regulatory particle, base subcomplex Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Nucleus, ProteasomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000084700 | 1 – 439 | 26S proteasome regulatory subunit 6AAdd BLAST | 439 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 9 | PhosphoserineBy similarity | 1 | |
Modified residuei | 376 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | Q63569 |
PaxDbi | Q63569 |
PeptideAtlasi | Q63569 |
PRIDEi | Q63569 |
PTM databases
iPTMneti | Q63569 |
PhosphoSitePlusi | Q63569 |
Interactioni
Subunit structurei
Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC3 and few additional components.
Interacts with PAAF1.
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248298, 7 interactors |
IntActi | Q63569, 3 interactors |
STRINGi | 10116.ENSRNOP00000015757 |
Family & Domainsi
Sequence similaritiesi
Belongs to the AAA ATPase family.Curated
Phylogenomic databases
eggNOGi | KOG0652, Eukaryota |
InParanoidi | Q63569 |
OrthoDBi | 571919at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR005937, 26S_Psome_P45-like IPR003593, AAA+_ATPase IPR041569, AAA_lid_3 IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR027417, P-loop_NTPase IPR032501, Prot_ATP_ID_OB |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF17862, AAA_lid_3, 1 hit PF16450, Prot_ATP_ID_OB, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01242, 26Sp45, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q63569-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNLLPTPESP VTRQEKMATV WDEAEQDGIG EEVLKMSTEE IVQRTRLLDS
60 70 80 90 100
EIKIMKSEVL RVTHELQAMK DKIKENSEKI KVNKTLPYLV SNVIELLDVD
110 120 130 140 150
PNDQEEDGAN IDLDSQRKGK CAVIKTSTRQ TYFLPVIGLV DAEKLKPGDL
160 170 180 190 200
VGVNKDSYLI LETLPTEYDS RVKAMEVDER PTEQYSDIGG LDKQIQELVE
210 220 230 240 250
AIVLPMNHKE KFENLGIQPP KGVLMYGPPG TGKTLLARAC AAQTKATFLK
260 270 280 290 300
LAGPQLVQMF IGDGAKLVRD AFALAKEKAP SIIFIDELDA IGTKRFDSEK
310 320 330 340 350
AGDREVQRTM LELLNQLDGF QPNTQVKVIA ATNRVDILDP ALLRSGRLDR
360 370 380 390 400
KIEFPMPNEE ARARIMQIHS RKMNVSPDVN YEELARCTDD FNGAQCKAVC
410 420 430
VEAGMIALRR GATELTHEDY MEGILEVQAK KKANLQYYA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ6P6U2 | Q6P6U2_RAT | 26S proteasome regulatory subunit 6... | Psmc3 rCG_27374 | 442 | Annotation score: |
Sequence cautioni
The sequence AAB70882 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 84 | K → N in AAB70882 (PubMed:9266764).Curated | 1 | |
Sequence conflicti | 317 | L → M in AAB70882 (PubMed:9266764).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D83522 mRNA Translation: BAA11939.1 U77918 mRNA Translation: AAB70882.1 Different initiation. |
RefSeqi | NP_113783.1, NM_031595.1 |
Genome annotation databases
GeneIDi | 29677 |
KEGGi | rno:29677 |
UCSCi | RGD:61905, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D83522 mRNA Translation: BAA11939.1 U77918 mRNA Translation: AAB70882.1 Different initiation. |
RefSeqi | NP_113783.1, NM_031595.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6EPC | electron microscopy | 12.30 | M | 1-439 | [»] | |
6EPD | electron microscopy | 15.40 | M | 1-439 | [»] | |
6EPE | electron microscopy | 12.80 | M | 1-439 | [»] | |
6EPF | electron microscopy | 11.80 | M | 1-439 | [»] | |
SMRi | Q63569 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 248298, 7 interactors |
IntActi | Q63569, 3 interactors |
STRINGi | 10116.ENSRNOP00000015757 |
PTM databases
iPTMneti | Q63569 |
PhosphoSitePlusi | Q63569 |
Proteomic databases
jPOSTi | Q63569 |
PaxDbi | Q63569 |
PeptideAtlasi | Q63569 |
PRIDEi | Q63569 |
Genome annotation databases
GeneIDi | 29677 |
KEGGi | rno:29677 |
UCSCi | RGD:61905, rat |
Organism-specific databases
CTDi | 5702 |
RGDi | 61905, Psmc3 |
Phylogenomic databases
eggNOGi | KOG0652, Eukaryota |
InParanoidi | Q63569 |
OrthoDBi | 571919at2759 |
Enzyme and pathway databases
Reactomei | R-RNO-1169091, Activation of NF-kappaB in B cells R-RNO-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-RNO-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-RNO-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-RNO-174154, APC/C:Cdc20 mediated degradation of Securin R-RNO-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-RNO-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-RNO-187577, SCF(Skp2)-mediated degradation of p27/p21 R-RNO-195253, Degradation of beta-catenin by the destruction complex R-RNO-202424, Downstream TCR signaling R-RNO-2467813, Separation of Sister Chromatids R-RNO-2871837, FCERI mediated NF-kB activation R-RNO-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-RNO-350562, Regulation of ornithine decarboxylase (ODC) R-RNO-382556, ABC-family proteins mediated transport R-RNO-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-RNO-4608870, Asymmetric localization of PCP proteins R-RNO-4641257, Degradation of AXIN R-RNO-4641258, Degradation of DVL R-RNO-5358346, Hedgehog ligand biogenesis R-RNO-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-RNO-5607764, CLEC7A (Dectin-1) signaling R-RNO-5610780, Degradation of GLI1 by the proteasome R-RNO-5610785, GLI3 is processed to GLI3R by the proteasome R-RNO-5632684, Hedgehog 'on' state R-RNO-5658442, Regulation of RAS by GAPs R-RNO-5668541, TNFR2 non-canonical NF-kB pathway R-RNO-5676590, NIK-->noncanonical NF-kB signaling R-RNO-5687128, MAPK6/MAPK4 signaling R-RNO-5689603, UCH proteinases R-RNO-5689880, Ub-specific processing proteases R-RNO-6798695, Neutrophil degranulation R-RNO-68827, CDT1 association with the CDC6:ORC:origin complex R-RNO-68949, Orc1 removal from chromatin R-RNO-69017, CDK-mediated phosphorylation and removal of Cdc6 R-RNO-69481, G2/M Checkpoints R-RNO-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-RNO-75815, Ubiquitin-dependent degradation of Cyclin D R-RNO-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-RNO-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-RNO-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-RNO-8939902, Regulation of RUNX2 expression and activity R-RNO-8941858, Regulation of RUNX3 expression and activity R-RNO-8948751, Regulation of PTEN stability and activity R-RNO-8951664, Neddylation R-RNO-9020702, Interleukin-1 signaling R-RNO-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
PROi | PR:Q63569 |
Family and domain databases
InterProi | View protein in InterPro IPR005937, 26S_Psome_P45-like IPR003593, AAA+_ATPase IPR041569, AAA_lid_3 IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR027417, P-loop_NTPase IPR032501, Prot_ATP_ID_OB |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF17862, AAA_lid_3, 1 hit PF16450, Prot_ATP_ID_OB, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01242, 26Sp45, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRS6A_RAT | |
Accessioni | Q63569Primary (citable) accession number: Q63569 Secondary accession number(s): P97638 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families