UniProtKB - Q63528 (RFA2_RAT)
Replication protein A 32 kDa subunit
Rpa2
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 74 – 148 | OBAdd BLAST | 75 |
GO - Molecular functioni
- damaged DNA binding Source: UniProtKB
- DNA binding Source: RGD
- enzyme binding Source: RGD
- G-rich strand telomeric DNA binding Source: RGD
- protein N-terminus binding Source: RGD
- protein phosphatase binding Source: RGD
- single-stranded DNA binding Source: RGD
- ubiquitin protein ligase binding Source: RGD
GO - Biological processi
- base-excision repair Source: UniProtKB
- DNA repair Source: GO_Central
- DNA replication Source: UniProtKB
- double-strand break repair via homologous recombination Source: UniProtKB
- mismatch repair Source: UniProtKB
- mitotic G1 DNA damage checkpoint Source: RGD
- nucleotide-excision repair Source: UniProtKB
- protein localization to chromosome Source: UniProtKB
- regulation of DNA damage checkpoint Source: UniProtKB
- regulation of double-strand break repair via homologous recombination Source: UniProtKB
- telomere maintenance Source: UniProtKB
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA damage, DNA recombination, DNA repair, DNA replication |
Enzyme and pathway databases
Reactomei | R-RNO-110312, Translesion synthesis by REV1 R-RNO-110314, Recognition of DNA damage by PCNA-containing replication complex R-RNO-110320, Translesion Synthesis by POLH R-RNO-174437, Removal of the Flap Intermediate from the C-strand R-RNO-176187, Activation of ATR in response to replication stress R-RNO-3371453, Regulation of HSF1-mediated heat shock response R-RNO-5358565, Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) R-RNO-5358606, Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta) R-RNO-5651801, PCNA-Dependent Long Patch Base Excision Repair R-RNO-5655862, Translesion synthesis by POLK R-RNO-5656121, Translesion synthesis by POLI R-RNO-5656169, Termination of translesion DNA synthesis R-RNO-5685938, HDR through Single Strand Annealing (SSA) R-RNO-5685942, HDR through Homologous Recombination (HRR) R-RNO-5693607, Processing of DNA double-strand break ends R-RNO-5693616, Presynaptic phase of homologous DNA pairing and strand exchange R-RNO-5696395, Formation of Incision Complex in GG-NER R-RNO-5696397, Gap-filling DNA repair synthesis and ligation in GG-NER R-RNO-5696400, Dual Incision in GG-NER R-RNO-6782135, Dual incision in TC-NER R-RNO-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-RNO-6783310, Fanconi Anemia Pathway R-RNO-6804756, Regulation of TP53 Activity through Phosphorylation R-RNO-68962, Activation of the pre-replicative complex R-RNO-69166, Removal of the Flap Intermediate R-RNO-69473, G2/M DNA damage checkpoint |
Names & Taxonomyi
Protein namesi | Recommended name: Replication protein A 32 kDa subunitShort name: RP-A p32 Alternative name(s): Replication factor A protein 2 Short name: RF-A protein 2 |
Gene namesi | Name:Rpa2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619714, Rpa2 |
Subcellular locationi
Nucleus
- DNA replication factor A complex Source: RGD
- nucleus Source: UniProtKB
- PML body Source: UniProtKB
Other locations
- chromatin Source: RGD
- chromosome, telomeric region Source: GO_Central
- site of double-strand break Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097273 | 1 – 270 | Replication protein A 32 kDa subunitAdd BLAST | 270 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 4 | Phosphoserine; by PRKDCBy similarity | 1 | |
Modified residuei | 8 | Phosphoserine; by PRKDCBy similarity | 1 | |
Modified residuei | 21 | Phosphothreonine; by PRKDCBy similarity | 1 | |
Modified residuei | 23 | Phosphoserine; by CDK2By similarity | 1 | |
Modified residuei | 29 | Phosphoserine; by CDK1By similarity | 1 | |
Modified residuei | 33 | Phosphoserine; by PRKDCBy similarity | 1 | |
Cross-linki | 37 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 38 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q63528 |
PaxDbi | Q63528 |
PRIDEi | Q63528 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000013005, Expressed in thymus and 22 other tissues |
Genevisiblei | Q63528, RN |
Interactioni
Subunit structurei
Component of the replication protein A complex (RPA/RP-A), a heterotrimeric complex composed of RPA1, RPA2 and RPA3.
Interacts with PRPF19; the PRP19-CDC5L complex is recruited to the sites of DNA repair where it ubiquitinates the replication protein A complex (RPA).
Interacts with SERTAD3.
Interacts with TIPIN.
Interacts with TIMELESS.
Interacts with PPP4R2; the interaction is direct, DNA damage-dependent and mediates the recruitment of the PP4 catalytic subunit PPP4C.
Interacts (hyperphosphorylated) with RAD51.
Interacts with SMARCAL1; the interaction is direct and mediates the recruitment to the RPA complex of SMARCAL1.
Interacts with RAD52 and XPA; those interactions are direct and associate RAD52 and XPA to the RPA complex.
Interacts with FBH1.
Interacts with ETAA1; the interaction is direct and promotes ETAA1 recruitment at stalled replication forks.
Interacts with DDI2 (By similarity).
By similarityGO - Molecular functioni
- enzyme binding Source: RGD
- protein N-terminus binding Source: RGD
- protein phosphatase binding Source: RGD
- ubiquitin protein ligase binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248725, 1 interactor |
STRINGi | 10116.ENSRNOP00000017549 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 187 – 270 | Interaction with RAD52, TIPIN, UNG and XPABy similarityAdd BLAST | 84 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3108, Eukaryota |
GeneTreei | ENSGT00390000010045 |
HOGENOMi | CLU_051033_1_0_1 |
InParanoidi | Q63528 |
OMAi | MQYAMAC |
OrthoDBi | 924826at2759 |
PhylomeDBi | Q63528 |
TreeFami | TF105242 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR012340, NA-bd_OB-fold IPR040260, RFA2-like IPR014646, Rfa2/RPA32 IPR014892, RPA_C IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR13989, PTHR13989, 1 hit |
Pfami | View protein in Pfam PF08784, RPA_C, 1 hit |
PIRSFi | PIRSF036949, RPA32, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF50249, SSF50249, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MWNSGFESFS SSSYGAAGGY TQSPGGFGSP TPSQAEKKSR ARAQHIVPCT
60 70 80 90 100
ISQLLSATLT DEVFKIGDVE ISQVTIVGII RHAEKAPTNI VYKIDDMTAA
110 120 130 140 150
PMDVRQWVDT DDTSGENTVV PPETYVKVAG HLRSFQNKKS LVAFKIIPLE
160 170 180 190 200
DMNEFTAHIL EVVNSHLMLS KANSQASVGR PSMSNPGMGE PGNFSGNNFM
210 220 230 240 250
PANGLTVVQN QVLNLIKACP RPEGLNFQDL RSQLQHMPVA SIKQAVDFLC
260 270
NEGHIYSTVD DDHFKSTDAE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC079180 mRNA Translation: AAH79180.1 X98490 mRNA Translation: CAA67116.1 |
RefSeqi | NP_067593.1, NM_021582.1 |
Genome annotation databases
Ensembli | ENSRNOT00000017549; ENSRNOP00000017549; ENSRNOG00000013005 |
GeneIDi | 59102 |
KEGGi | rno:59102 |
UCSCi | RGD:619714, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC079180 mRNA Translation: AAH79180.1 X98490 mRNA Translation: CAA67116.1 |
RefSeqi | NP_067593.1, NM_021582.1 |
3D structure databases
SMRi | Q63528 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248725, 1 interactor |
STRINGi | 10116.ENSRNOP00000017549 |
Proteomic databases
jPOSTi | Q63528 |
PaxDbi | Q63528 |
PRIDEi | Q63528 |
Genome annotation databases
Ensembli | ENSRNOT00000017549; ENSRNOP00000017549; ENSRNOG00000013005 |
GeneIDi | 59102 |
KEGGi | rno:59102 |
UCSCi | RGD:619714, rat |
Organism-specific databases
CTDi | 6118 |
RGDi | 619714, Rpa2 |
Phylogenomic databases
eggNOGi | KOG3108, Eukaryota |
GeneTreei | ENSGT00390000010045 |
HOGENOMi | CLU_051033_1_0_1 |
InParanoidi | Q63528 |
OMAi | MQYAMAC |
OrthoDBi | 924826at2759 |
PhylomeDBi | Q63528 |
TreeFami | TF105242 |
Enzyme and pathway databases
Reactomei | R-RNO-110312, Translesion synthesis by REV1 R-RNO-110314, Recognition of DNA damage by PCNA-containing replication complex R-RNO-110320, Translesion Synthesis by POLH R-RNO-174437, Removal of the Flap Intermediate from the C-strand R-RNO-176187, Activation of ATR in response to replication stress R-RNO-3371453, Regulation of HSF1-mediated heat shock response R-RNO-5358565, Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) R-RNO-5358606, Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta) R-RNO-5651801, PCNA-Dependent Long Patch Base Excision Repair R-RNO-5655862, Translesion synthesis by POLK R-RNO-5656121, Translesion synthesis by POLI R-RNO-5656169, Termination of translesion DNA synthesis R-RNO-5685938, HDR through Single Strand Annealing (SSA) R-RNO-5685942, HDR through Homologous Recombination (HRR) R-RNO-5693607, Processing of DNA double-strand break ends R-RNO-5693616, Presynaptic phase of homologous DNA pairing and strand exchange R-RNO-5696395, Formation of Incision Complex in GG-NER R-RNO-5696397, Gap-filling DNA repair synthesis and ligation in GG-NER R-RNO-5696400, Dual Incision in GG-NER R-RNO-6782135, Dual incision in TC-NER R-RNO-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-RNO-6783310, Fanconi Anemia Pathway R-RNO-6804756, Regulation of TP53 Activity through Phosphorylation R-RNO-68962, Activation of the pre-replicative complex R-RNO-69166, Removal of the Flap Intermediate R-RNO-69473, G2/M DNA damage checkpoint |
Miscellaneous databases
PROi | PR:Q63528 |
Gene expression databases
Bgeei | ENSRNOG00000013005, Expressed in thymus and 22 other tissues |
Genevisiblei | Q63528, RN |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR012340, NA-bd_OB-fold IPR040260, RFA2-like IPR014646, Rfa2/RPA32 IPR014892, RPA_C IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR13989, PTHR13989, 1 hit |
Pfami | View protein in Pfam PF08784, RPA_C, 1 hit |
PIRSFi | PIRSF036949, RPA32, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF50249, SSF50249, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RFA2_RAT | |
Accessioni | Q63528Primary (citable) accession number: Q63528 Secondary accession number(s): Q6AY60 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | April 26, 2005 | |
Last modified: | December 2, 2020 | |
This is version 142 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families