UniProtKB - Q63433 (PKN1_RAT)
Serine/threonine-protein kinase N1
Pkn1
Functioni
Catalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 648 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 744 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 625 – 633 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- androgen receptor binding Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- calcium-dependent protein kinase C activity Source: UniProtKB-EC
- chromatin binding Source: UniProtKB
- GTP-Rho binding Source: UniProtKB
- histone binding Source: UniProtKB
- histone deacetylase binding Source: UniProtKB
- histone kinase activity (H3-T11 specific) Source: UniProtKB
- nuclear receptor transcription coactivator activity Source: UniProtKB
- protein kinase activity Source: RGD
- protein kinase C binding Source: RGD
- protein serine/threonine kinase activity Source: UniProtKB
- Rac GTPase binding Source: UniProtKB
GO - Biological processi
- B cell apoptotic process Source: RGD
- B cell homeostasis Source: RGD
- epithelial cell migration Source: UniProtKB
- histone H3-T11 phosphorylation Source: UniProtKB
- hyperosmotic response Source: RGD
- intracellular signal transduction Source: GO_Central
- negative regulation of B cell proliferation Source: RGD
- negative regulation of protein kinase activity Source: RGD
- negative regulation of protein phosphorylation Source: RGD
- peptidyl-serine phosphorylation Source: GO_Central
- protein phosphorylation Source: RGD
- regulation of cell motility Source: UniProtKB
- regulation of germinal center formation Source: RGD
- regulation of immunoglobulin production Source: RGD
- regulation of transcription by RNA polymerase II Source: UniProtKB
- renal system process Source: RGD
- spleen development Source: RGD
Keywordsi
Molecular function | Chromatin regulator, Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Transcription, Transcription regulation |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.13, 5301 |
Reactomei | R-RNO-5625740, RHO GTPases activate PKNs R-RNO-5625886, Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein kinase N1 (EC:2.7.11.131 Publication)Alternative name(s): Protease-activated kinase 1 Short name: PAK-1 Protein kinase C-like 1 Protein kinase C-like PKN Protein-kinase C-related kinase 1 Serine-threonine protein kinase N |
Gene namesi | Name:Pkn1 Synonyms:Pkn, Prk1, Prkcl1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 69308, Pkn1 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Peripheral membrane protein 1 Publication
Nucleus
- Nucleus By similarity
Endosome
- Endosome By similarity
Other locations
- Cytoplasm 1 Publication
- Cleavage furrow By similarity
- Midbody By similarity
Note: Associates with chromatin in a ligand-dependent manner (By similarity). Localization to endosomes is mediated via its interaction with RHOB. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis (By similarity). Association to the cell membrane is dependent on Ser-377 phosphorylation.By similarity1 Publication
Endosome
- endosome Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- cleavage furrow Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- cytoplasmic vesicle Source: RGD
- midbody Source: UniProtKB
- protein-containing complex Source: Ensembl
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Endosome, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000055721 | 2 – 946 | Serine/threonine-protein kinase N1Add BLAST | 945 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 69 | PhosphoserineBy similarity | 1 | |
Modified residuei | 377 | Phosphoserine1 Publication | 1 | |
Modified residuei | 451 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 536 | PhosphoserineBy similarity | 1 | |
Modified residuei | 540 | PhosphoserineBy similarity | 1 | |
Modified residuei | 543 | PhosphoserineBy similarity | 1 | |
Modified residuei | 562 | PhosphoserineBy similarity | 1 | |
Modified residuei | 565 | PhosphoserineBy similarity | 1 | |
Modified residuei | 612 | PhosphoserineBy similarity | 1 | |
Modified residuei | 778 | Phosphothreonine; by PDPK1By similarity | 1 | |
Modified residuei | 782 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 918 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 920 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 108 – 109 | Cleavage; by caspase-3By similarity | 2 | |
Sitei | 457 – 458 | Cleavage; by caspase-3By similarity | 2 | |
Sitei | 561 – 562 | Cleavage; by caspase-3By similarity | 2 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | Q63433 |
PaxDbi | Q63433 |
PRIDEi | Q63433 |
PTM databases
iPTMneti | Q63433 |
PhosphoSitePlusi | Q63433 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000004131, Expressed in thymus and 20 other tissues |
Genevisiblei | Q63433, RN |
Interactioni
Subunit structurei
Interacts with ZFAND6 (By similarity).
Interacts with ANDR.
Interacts with PRKCB.
Interacts (via REM 1 and REM 2 repeats) with RAC1.
Interacts (via REM 1 repeat) with RHOA.
Interacts with RHOB.
Interacts (via C-terminus) with PDPK1.
Interacts with CCNT2; enhances MYOD1-dependent transcription.
Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and ZAK (By similarity).
By similarityGO - Molecular functioni
- androgen receptor binding Source: UniProtKB
- GTP-Rho binding Source: UniProtKB
- histone binding Source: UniProtKB
- histone deacetylase binding Source: UniProtKB
- protein kinase C binding Source: RGD
- Rac GTPase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 248010, 1 interactor |
DIPi | DIP-60097N |
IntActi | Q63433, 9 interactors |
STRINGi | 10116.ENSRNOP00000005770 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 25 – 100 | REM-1 1PROSITE-ProRule annotationAdd BLAST | 76 | |
Domaini | 114 – 193 | REM-1 2PROSITE-ProRule annotationAdd BLAST | 80 | |
Domaini | 202 – 283 | REM-1 3PROSITE-ProRule annotationAdd BLAST | 82 | |
Domaini | 310 – 473 | C2PROSITE-ProRule annotationAdd BLAST | 164 | |
Domaini | 619 – 878 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 260 | |
Domaini | 879 – 946 | AGC-kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 68 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG0694, Eukaryota |
GeneTreei | ENSGT00940000154990 |
HOGENOMi | CLU_000288_132_1_1 |
InParanoidi | Q63433 |
OMAi | WNPSPSG |
OrthoDBi | 520651at2759 |
Family and domain databases
CDDi | cd08687, C2_PKN-like, 1 hit cd11630, HR1_PKN1_2, 1 hit cd11622, HR1_PKN_1, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR037784, C2_PKN IPR011072, HR1_rho-bd IPR036274, HR1_rpt_sf IPR011009, Kinase-like_dom_sf IPR017892, Pkinase_C IPR037317, PKN1_HR1_2 IPR037313, PKN_HR1_1 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF02185, HR1, 3 hits PF00069, Pkinase, 1 hit PF00433, Pkinase_C, 1 hit |
SMARTi | View protein in SMART SM00742, Hr1, 3 hits SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF46585, SSF46585, 3 hits SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS50004, C2, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS51860, REM_1, 3 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MAGDAVQSEP RSWSLLEQLG LAGADLAAPG VQQQLELERE RLKREIRKEL
60 70 80 90 100
KLKEGAENLR RATTDLGRSL APVELLLRGS ARRLDLLHQQ LQELHAHVVL
110 120 130 140 150
PDPTAGSDAP QSLAEGSPVC SSTNLSRVAG LEKQLAIELK VKQGAENMIQ
160 170 180 190 200
TYSNGSTKDR KLLLTAQQML QDSKTKIDII RMQLRRALQA LQAGQLESQA
210 220 230 240 250
APDEAHGDPD LGAVELRIEE LRHHFRVEHA VAEGAKNVLR LLSAAKAPDR
260 270 280 290 300
KAVSEAQEKL TESNQKLGLL RESLERRLGE LPADHPKGRL LREELTAASS
310 320 330 340 350
AAFSAILPGP FPATHYSTLS KPAPLTGTLE VRVVGCKNLP ETIPWSPPPS
360 370 380 390 400
VGASGTPDSR TPFLSRPARG LYNRSGSLSG RSSLKGEAEN STEVSTVLKL
410 420 430 440 450
DNTVVGQTAW KPCGPNAWDQ SFTLELERAR ELELAVFWRD QRGLCALKFL
460 470 480 490 500
KLEDFLDNER HEVQLDMEPQ GCLVAEVTFR NPIIERIPRL QRQKKIFSKQ
510 520 530 540 550
QGQTFQRARQ MNIDVATWVR LLRRLIPNAV ATGSFSPNAS PGSEIRSTGD
560 570 580 590 600
ISMEKLNLGA DSDSSSQKSP AGLPSTSCSL SSPTHESTTS PELPSETQET
610 620 630 640 650
PGPGLCSPLR KSPLTLEDFK FLAVLGRGHF GKVLLSEFHS SGELFAIKAL
660 670 680 690 700
KKGDIVARDE VESLMCEKRI LATVTRAGHP FLVNLFGCFQ TPEHVCFVME
710 720 730 740 750
YSAGGDLMLH IHSDVFSEPR AVFYSACVVL GLQFLHEHKI VYRDLKLDNL
760 770 780 790 800
LLDTEGYVKI ADFGLCKEGM GYGDRTSTFC GTPEFLAPEV LTDTSYTRAV
810 820 830 840 850
DWWGLGVLLY EMLVGESPFP GDDEEEVFDS IVNDEVRYPR FLSAEAIGIM
860 870 880 890 900
RRLLRRNPER RLGSTERDAE DVKKQPFFRT LDWDALLARR LPPPFVPTLS
910 920 930 940
GRTDVSNFDE EFTGEAPTLS PPRDARPLTA AEQAAFRDFD FVAGGY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 298 | A → R in AAL31374 (PubMed:8943281).Curated | 1 | |
Sequence conflicti | 510 | Q → H in BAA05168 (PubMed:8135837).Curated | 1 | |
Sequence conflicti | 650 | L → V in AAL31374 (PubMed:8943281).Curated | 1 | |
Sequence conflicti | 722 | V → G in BAA05168 (PubMed:8135837).Curated | 1 | |
Sequence conflicti | 808 | L → F in BAA05168 (PubMed:8135837).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26180 mRNA Translation: BAA05168.1 L35634 mRNA Translation: AAL31374.1 BC061836 mRNA Translation: AAH61836.1 |
PIRi | JC2130 |
RefSeqi | NP_058871.2, NM_017175.2 |
Genome annotation databases
Ensembli | ENSRNOT00000005770; ENSRNOP00000005770; ENSRNOG00000004131 |
GeneIDi | 29355 |
KEGGi | rno:29355 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26180 mRNA Translation: BAA05168.1 L35634 mRNA Translation: AAL31374.1 BC061836 mRNA Translation: AAH61836.1 |
PIRi | JC2130 |
RefSeqi | NP_058871.2, NM_017175.2 |
3D structure databases
SMRi | Q63433 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248010, 1 interactor |
DIPi | DIP-60097N |
IntActi | Q63433, 9 interactors |
STRINGi | 10116.ENSRNOP00000005770 |
PTM databases
iPTMneti | Q63433 |
PhosphoSitePlusi | Q63433 |
Proteomic databases
jPOSTi | Q63433 |
PaxDbi | Q63433 |
PRIDEi | Q63433 |
Genome annotation databases
Ensembli | ENSRNOT00000005770; ENSRNOP00000005770; ENSRNOG00000004131 |
GeneIDi | 29355 |
KEGGi | rno:29355 |
Organism-specific databases
CTDi | 5585 |
RGDi | 69308, Pkn1 |
Phylogenomic databases
eggNOGi | KOG0694, Eukaryota |
GeneTreei | ENSGT00940000154990 |
HOGENOMi | CLU_000288_132_1_1 |
InParanoidi | Q63433 |
OMAi | WNPSPSG |
OrthoDBi | 520651at2759 |
Enzyme and pathway databases
BRENDAi | 2.7.11.13, 5301 |
Reactomei | R-RNO-5625740, RHO GTPases activate PKNs R-RNO-5625886, Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 |
Miscellaneous databases
PROi | PR:Q63433 |
Gene expression databases
Bgeei | ENSRNOG00000004131, Expressed in thymus and 20 other tissues |
Genevisiblei | Q63433, RN |
Family and domain databases
CDDi | cd08687, C2_PKN-like, 1 hit cd11630, HR1_PKN1_2, 1 hit cd11622, HR1_PKN_1, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR037784, C2_PKN IPR011072, HR1_rho-bd IPR036274, HR1_rpt_sf IPR011009, Kinase-like_dom_sf IPR017892, Pkinase_C IPR037317, PKN1_HR1_2 IPR037313, PKN_HR1_1 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF02185, HR1, 3 hits PF00069, Pkinase, 1 hit PF00433, Pkinase_C, 1 hit |
SMARTi | View protein in SMART SM00742, Hr1, 3 hits SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF46585, SSF46585, 3 hits SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS50004, C2, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS51860, REM_1, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PKN1_RAT | |
Accessioni | Q63433Primary (citable) accession number: Q63433 Secondary accession number(s): Q6P748, Q8VIJ2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | May 18, 2010 | |
Last modified: | December 2, 2020 | |
This is version 177 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families