UniProtKB - Q63406 (MCF2L_RAT)
Guanine nucleotide exchange factor DBS
Mcf2l
Functioni
Guanine nucleotide exchange factor that catalyzes guanine nucleotide exchange on RHOA and CDC42, and thereby contributes to the regulation of RHOA and CDC42 signaling pathways. Seems to lack activity with RAC1. Becomes activated and highly tumorigenic by truncation of the N-terminus.
1 PublicationGO - Molecular functioni
- 1-phosphatidylinositol binding Source: RGD
- guanyl-nucleotide exchange factor activity Source: UniProtKB
- phosphatidylinositol binding Source: RGD
GO - Biological processi
- positive regulation of Rho protein signal transduction Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: RGD
- Rho protein signal transduction Source: RGD
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Ligand | Lipid-binding |
Enzyme and pathway databases
Reactomei | R-RNO-193648, NRAGE signals death through JNK R-RNO-416482, G alpha (12/13) signalling events R-RNO-8980692, RHOA GTPase cycle R-RNO-9013026, RHOB GTPase cycle R-RNO-9013106, RHOC GTPase cycle R-RNO-9013148, CDC42 GTPase cycle R-RNO-9013149, RAC1 GTPase cycle R-RNO-9013408, RHOG GTPase cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Guanine nucleotide exchange factor DBSAlternative name(s): DBL's big sister MCF2-transforming sequence-like protein OST oncogene1 Publication |
Gene namesi | Name:Mcf2l Synonyms:Ost1 Publication |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619782, Mcf2l |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB
- extrinsic component of membrane Source: UniProtKB
- lamellipodium Source: RGD
- membrane Source: RGD
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080937 | 1 – 1149 | Guanine nucleotide exchange factor DBSAdd BLAST | 1149 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 457 | PhosphoserineCombined sources | 1 | |
Modified residuei | 462 | PhosphoserineCombined sources | 1 | |
Modified residuei | 471 | PhosphoserineCombined sources | 1 | |
Modified residuei | 480 | PhosphoserineCombined sources | 1 | |
Modified residuei | 621 | PhosphoserineBy similarity | 1 | |
Modified residuei | 622 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1033 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1034 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1041 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1042 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q63406 |
PRIDEi | Q63406 |
PTM databases
iPTMneti | Q63406 |
PhosphoSitePlusi | Q63406 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000028426, Expressed in frontal cortex and 20 other tissues |
ExpressionAtlasi | Q63406, baseline and differential |
Genevisiblei | Q63406, RN |
Interactioni
Subunit structurei
Interacts with GTP-bound RAC1 (PubMed:7957046).
Interacts with CDC42.
Interacts with RHOA.
Interacts with CCPG1, which results in specific inhibition of its exchange activity toward RHOA, but does not affect its activity on CDC42 (By similarity).
By similarity1 PublicationProtein-protein interaction databases
CORUMi | Q63406 |
STRINGi | 10116.ENSRNOP00000023352 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 52 – 224 | CRAL-TRIOPROSITE-ProRule annotationAdd BLAST | 173 | |
Repeati | 355 – 454 | SpectrinAdd BLAST | 100 | |
Domaini | 632 – 812 | DHPROSITE-ProRule annotationAdd BLAST | 181 | |
Domaini | 830 – 946 | PHPROSITE-ProRule annotationAdd BLAST | 117 | |
Domaini | 1055 – 1116 | SH3PROSITE-ProRule annotationAdd BLAST | 62 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 555 – 627 | DisorderedSequence analysisAdd BLAST | 73 | |
Regioni | 956 – 1033 | DisorderedSequence analysisAdd BLAST | 78 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 503 – 528 | Sequence analysisAdd BLAST | 26 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 578 – 598 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 601 – 627 | Basic and acidic residuesSequence analysisAdd BLAST | 27 | |
Compositional biasi | 961 – 982 | Polar residuesSequence analysisAdd BLAST | 22 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coil, SH3 domainPhylogenomic databases
eggNOGi | KOG4240, Eukaryota |
GeneTreei | ENSGT00940000157874 |
InParanoidi | Q63406 |
Family and domain databases
CDDi | cd01227, PH_Dbs, 1 hit cd00160, RhoGEF, 1 hit cd00170, SEC14, 1 hit cd11857, SH3_DBS, 1 hit cd00176, SPEC, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR001251, CRAL-TRIO_dom IPR036865, CRAL-TRIO_dom_sf IPR035899, DBL_dom_sf IPR035534, DBS_PH IPR035532, DBS_SH3 IPR000219, DH-domain IPR001331, GDS_CDC24_CS IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR018159, Spectrin/alpha-actinin IPR002017, Spectrin_repeat |
Pfami | View protein in Pfam PF13716, CRAL_TRIO_2, 1 hit PF00169, PH, 1 hit PF00621, RhoGEF, 1 hit PF00435, Spectrin, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00325, RhoGEF, 1 hit SM00516, SEC14, 1 hit SM00326, SH3, 1 hit SM00150, SPEC, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit SSF50044, SSF50044, 1 hit SSF52087, SSF52087, 1 hit |
PROSITEi | View protein in PROSITE PS50191, CRAL_TRIO, 1 hit PS00741, DH_1, 1 hit PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 1 hit PS50002, SH3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSNCWCFIFC KERVRSNSSS PQHDGTSREE ADHQVDVSDG IRLVPDKAEA
60 70 80 90 100
TMATASDEIM HQDIVPLCAA DIQEQLKKRF AYLSGGRGQD GSPVITFPDY
110 120 130 140 150
PAFSEIPDKE FQNVMTYLTS IPSLQDAGIG FILVIDRRQD KWTSVKASVL
160 170 180 190 200
RIAASFPANL QLVLVLRPTG FFQRTLSDIA FKFNRDEFKM KVPVMMLSSV
210 220 230 240 250
PELHGYIDKS QLTEDLGGTL DYCHSRWLCH RTAIESFALM VKQTAQMLQA
260 270 280 290 300
FGTELAETEL PNDVQSTSLV LSAHTEKKAK VKEDLQLALT EGNSILESLR
310 320 330 340 350
EPLAESIVHS VNQDQLDNQA TVKRLLTQLN ETEAAFDEFW AKHQQKLEQC
360 370 380 390 400
LQLRHFEQGF REVKTALDSM SQKIAAFTDV GNSLAHVQHL LKDLTTFEEK
410 420 430 440 450
SSVAVDKARA LSLEGQQLIE NRHYAVDSIH PKCEELQHLC DHFASEVTRR
460 470 480 490 500
RDLLSKSLEL HSLLETSMKW SDEGIFLLAS QPVDKCQSQD GAEAALQEIE
510 520 530 540 550
KFLETGAENK IQELNKIYKE YECILNQDLL EHVQKVFQKQ ESTEEMFHRR
560 570 580 590 600
QASLKKLAAK QTRPVQPVAP RPEALTKSPS PSPGSWRSSE NSSSEGNALR
610 620 630 640 650
RGPYRRAKSE MSEPRQGRTS STGEEEESLA ILRRHVMNEL LDTERAYVEE
660 670 680 690 700
LLCVLEGYAA EMDNPLMAHL ISTGLQNKKN ILFGNMEEIY HFHNRIFLRE
710 720 730 740 750
LESCIDCPEL VGRCFLERME EFQIYEKYCQ NKPRSESLWR QCSDCPFFQE
760 770 780 790 800
CQKKLDHKLS LDSYLLKPVQ RITKYQLLLK EMLKYSKHCE GAEDLQEALS
810 820 830 840 850
SILGILKAVN DSMHLIAITG YDGNLGDLGK LLMQGSFSVW TDHKKGHTKV
860 870 880 890 900
KELARFKPMQ RHLFLHEKAV LFCKKREENG EGYEKAPSYS YKQSLNMTAV
910 920 930 940 950
GITENVKGDT KKFEIWYNAR EEVYIIQAPT PEIKAAWVNE IRKVLTSQLQ
960 970 980 990 1000
ACREASQHRA LEQSHSLPLP TPASTSPTKG STRNVKKLED RKTDPLCLEG
1010 1020 1030 1040 1050
CVSSSLPKPP EKGKGWSKTS HSLEAPEEDG GWSSAEELIN SSDAEEDGGV
1060 1070 1080 1090 1100
GPRKLVPGKY TVLMDGEKGG SDTLAMRSGD MVEVVEEGTE GLWYVRDLTS
1110 1120 1130 1140
SKEGWVPASS LATLLGKSSS AQCLSSSGKT HCARQLCPEP AKILSPEPV
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2KA20 | A0A0G2KA20_RAT | Guanine nucleotide exchange factor ... | Mcf2l | 1,179 | Annotation score: | ||
A0A0G2JU75 | A0A0G2JU75_RAT | Guanine nucleotide exchange factor ... | Mcf2l | 872 | Annotation score: | ||
D4A7F3 | D4A7F3_RAT | Guanine nucleotide exchange factor ... | Mcf2l | 1,097 | Annotation score: | ||
A0A8I5Y6G8 | A0A8I5Y6G8_RAT | Guanine nucleotide exchange factor ... | Mcf2l | 1,175 | Annotation score: | ||
A0A8I5ZWU6 | A0A8I5ZWU6_RAT | Guanine nucleotide exchange factor ... | Mcf2l | 1,249 | Annotation score: | ||
A0A8I5ZZZ7 | A0A8I5ZZZ7_RAT | Guanine nucleotide exchange factor ... | Mcf2l | 1,224 | Annotation score: |
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC117058 Genomic DNA No translation available. Z35654 mRNA Translation: CAA84713.1 Sequence problems. |
PIRi | S51620 |
Genome annotation databases
Ensembli | ENSRNOT00000079096; ENSRNOP00000068993; ENSRNOG00000028426 |
UCSCi | RGD:619782, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC117058 Genomic DNA No translation available. Z35654 mRNA Translation: CAA84713.1 Sequence problems. |
PIRi | S51620 |
3D structure databases
AlphaFoldDBi | Q63406 |
SMRi | Q63406 |
ModBasei | Search... |
Protein-protein interaction databases
CORUMi | Q63406 |
STRINGi | 10116.ENSRNOP00000023352 |
PTM databases
iPTMneti | Q63406 |
PhosphoSitePlusi | Q63406 |
Proteomic databases
PaxDbi | Q63406 |
PRIDEi | Q63406 |
Genome annotation databases
Ensembli | ENSRNOT00000079096; ENSRNOP00000068993; ENSRNOG00000028426 |
UCSCi | RGD:619782, rat |
Organism-specific databases
RGDi | 619782, Mcf2l |
Phylogenomic databases
eggNOGi | KOG4240, Eukaryota |
GeneTreei | ENSGT00940000157874 |
InParanoidi | Q63406 |
Enzyme and pathway databases
Reactomei | R-RNO-193648, NRAGE signals death through JNK R-RNO-416482, G alpha (12/13) signalling events R-RNO-8980692, RHOA GTPase cycle R-RNO-9013026, RHOB GTPase cycle R-RNO-9013106, RHOC GTPase cycle R-RNO-9013148, CDC42 GTPase cycle R-RNO-9013149, RAC1 GTPase cycle R-RNO-9013408, RHOG GTPase cycle |
Miscellaneous databases
PROi | PR:Q63406 |
Gene expression databases
Bgeei | ENSRNOG00000028426, Expressed in frontal cortex and 20 other tissues |
ExpressionAtlasi | Q63406, baseline and differential |
Genevisiblei | Q63406, RN |
Family and domain databases
CDDi | cd01227, PH_Dbs, 1 hit cd00160, RhoGEF, 1 hit cd00170, SEC14, 1 hit cd11857, SH3_DBS, 1 hit cd00176, SPEC, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR001251, CRAL-TRIO_dom IPR036865, CRAL-TRIO_dom_sf IPR035899, DBL_dom_sf IPR035534, DBS_PH IPR035532, DBS_SH3 IPR000219, DH-domain IPR001331, GDS_CDC24_CS IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR018159, Spectrin/alpha-actinin IPR002017, Spectrin_repeat |
Pfami | View protein in Pfam PF13716, CRAL_TRIO_2, 1 hit PF00169, PH, 1 hit PF00621, RhoGEF, 1 hit PF00435, Spectrin, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00325, RhoGEF, 1 hit SM00516, SEC14, 1 hit SM00326, SH3, 1 hit SM00150, SPEC, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit SSF50044, SSF50044, 1 hit SSF52087, SSF52087, 1 hit |
PROSITEi | View protein in PROSITE PS50191, CRAL_TRIO, 1 hit PS00741, DH_1, 1 hit PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 1 hit PS50002, SH3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MCF2L_RAT | |
Accessioni | Q63406Primary (citable) accession number: Q63406 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | April 20, 2010 | |
Last modified: | May 25, 2022 | |
This is version 161 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families