UniProtKB - Q63398 (COE1_RAT)
Protein
Transcription factor COE1
Gene
Ebf1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Key pioneer transcription factor of B-cell specification and commitment. Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Operates in a transcription factor network to activate B-cell-specific genes and repress genes associated with alternative cell fates. For instance, positively regulates many B-cell specific genes including BCR or CD40 while repressing genes that direct cells into alternative lineages, including GATA3 and TCF7 for the T-cell lineage. In addition to its role during lymphopoiesis, controls the thermogenic gene program in adipocytes during development and in response to environmental cold.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 151 – 170 | C5-typeBy similarityAdd BLAST | 20 |
GO - Molecular functioni
- C2H2 zinc finger domain binding Source: RGD
- DNA binding Source: RGD
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: RGD
- DNA-binding transcription factor activity Source: RGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: RGD
GO - Biological processi
- multicellular organism development Source: UniProtKB-KW
- positive regulation of transcription, DNA-templated Source: RGD
- positive regulation of transcription by RNA polymerase II Source: RGD
- regulation of transcription, DNA-templated Source: RGD
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor COE1Short name: O/E-1 Short name: OE-1 Alternative name(s): Early B-cell factor Olf-1 Olfactory neuronal transcription factor |
Gene namesi | Name:Ebf1 Synonyms:Coe1, Ebf, Olf1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620953, Ebf1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000107827 | 1 – 584 | Transcription factor COE1Add BLAST | 584 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Cross-linki | 16 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 16 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity |
Keywords - PTMi
Acetylation, Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | Q63398 |
Expressioni
Tissue specificityi
Expressed exclusively in olfactory receptor neurons and their precursors.
Gene expression databases
Bgeei | ENSRNOG00000028845, Expressed in spleen and 19 other tissues |
ExpressionAtlasi | Q63398, baseline and differential |
Genevisiblei | Q63398, RN |
Interactioni
Subunit structurei
Homodimer (By similarity).
Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes (PubMed:9151733).
Interacts with CCR4-NOT component CNOT3 (By similarity).
By similarity1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 163 | Interaction with DNABy similarity | 1 | |
Sitei | 172 | Interaction with DNABy similarity | 1 |
GO - Molecular functioni
- C2H2 zinc finger domain binding Source: RGD
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 250478, 1 interactor |
STRINGi | 10116.ENSRNOP00000068319 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 255 – 338 | IPT/TIGAdd BLAST | 84 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 63 – 66 | Interaction with DNABy similarity | 4 | |
Regioni | 197 – 204 | Interaction with DNABy similarity | 8 | |
Regioni | 236 – 239 | Interaction with DNABy similarity | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 455 – 543 | Pro/Ser/Thr-richAdd BLAST | 89 |
Sequence similaritiesi
Belongs to the COE family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 151 – 170 | C5-typeBy similarityAdd BLAST | 20 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3836, Eukaryota |
GeneTreei | ENSGT00950000182859 |
HOGENOMi | CLU_016320_3_1_1 |
InParanoidi | Q63398 |
OMAi | DDLFLHR |
OrthoDBi | 817293at2759 |
PhylomeDBi | Q63398 |
TreeFami | TF313391 |
Family and domain databases
CDDi | cd11606, COE_DBD, 1 hit cd01175, IPT_COE, 1 hit |
Gene3Di | 2.60.40.10, 1 hit 2.60.40.3180, 1 hit 4.10.280.10, 2 hits |
InterProi | View protein in InterPro IPR032200, COE_DBD IPR038173, COE_DBD_sf IPR032201, COE_HLH IPR038006, COE_IPT IPR036638, HLH_DNA-bd_sf IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR002909, IPT_dom IPR003523, Transcription_factor_COE IPR018350, Transcription_factor_COE_CS |
PANTHERi | PTHR10747, PTHR10747, 1 hit |
Pfami | View protein in Pfam PF16422, COE1_DBD, 1 hit PF16423, COE1_HLH, 1 hit PF01833, TIG, 1 hit |
SMARTi | View protein in SMART SM00429, IPT, 1 hit |
SUPFAMi | SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS01345, COE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q63398-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFGIQESIQR SGSSMKEEPL GSGMNAVRTW MQGAGVLDAN TAAQSGVGLA
60 70 80 90 100
RAHFEKQPPS NLRKSNFFHF VLALYDRQGQ PVEIERTAFV GFVEKEKEAN
110 120 130 140 150
SEKTNNGIHY RLQLLYSNGI RTEQDFYVRL IDSMTKQAIV YEGQDKNPEM
160 170 180 190 200
CRVLLTHEIM CSRCCDKKSC GNRNETPSDP VIIDRFFLKF FLKCNQNCLK
210 220 230 240 250
NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH GRRARRLDPS
260 270 280 290 300
EAATPCIKAI SPSEGWTTGG ATVIIIGDNF FDGLQVIFGT MLVWSELITP
310 320 330 340 350
HAIRVQTPPR HIPGVVEVTL SYKSKQFCKG TPGRFIYTAL NEPTIDYGFQ
360 370 380 390 400
RLQKVIPRHP GDPERLPKEV ILKRAADLVE ALYGMPHNNQ EIILKRAADI
410 420 430 440 450
AEALYSVPRN HNQLPALANT SVHAGMMGVN SFSGQLAVNV SEASQATNQG
460 470 480 490 500
FTRNSSSVSP HGYVPSTTPQ QTNYNSVTTS MNGYGSAAMS NLGGSPTFLN
510 520 530 540 550
GSAANSPYAI VPSSPTMASS TSLPSNCSSS SGIFSFSPAN MVSAVKQKSA
560 570 580
FAPVVRPQTS PPPTCTSTNG NSLQAISGMI VPPM
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A096MK03 | A0A096MK03_RAT | Transcription factor COE1 | Ebf1 | 159 | Annotation score: | ||
M0RCU9 | M0RCU9_RAT | Transcription factor COE1 | Ebf1 | 406 | Annotation score: | ||
A0A096MIU2 | A0A096MIU2_RAT | Transcription factor COE1 | Ebf1 | 171 | Annotation score: |
Sequence cautioni
The sequence AAA41759 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L24051 mRNA Translation: AAA41759.1 Different initiation. |
PIRi | S35069 |
RefSeqi | NP_446272.2, NM_053820.2 |
Genome annotation databases
Ensembli | ENSRNOT00000076664; ENSRNOP00000068319; ENSRNOG00000028845 |
GeneIDi | 116543 |
KEGGi | rno:116543 |
UCSCi | RGD:620953, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L24051 mRNA Translation: AAA41759.1 Different initiation. |
PIRi | S35069 |
RefSeqi | NP_446272.2, NM_053820.2 |
3D structure databases
SMRi | Q63398 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 250478, 1 interactor |
STRINGi | 10116.ENSRNOP00000068319 |
Proteomic databases
PaxDbi | Q63398 |
Genome annotation databases
Ensembli | ENSRNOT00000076664; ENSRNOP00000068319; ENSRNOG00000028845 |
GeneIDi | 116543 |
KEGGi | rno:116543 |
UCSCi | RGD:620953, rat |
Organism-specific databases
CTDi | 1879 |
RGDi | 620953, Ebf1 |
Phylogenomic databases
eggNOGi | KOG3836, Eukaryota |
GeneTreei | ENSGT00950000182859 |
HOGENOMi | CLU_016320_3_1_1 |
InParanoidi | Q63398 |
OMAi | DDLFLHR |
OrthoDBi | 817293at2759 |
PhylomeDBi | Q63398 |
TreeFami | TF313391 |
Miscellaneous databases
PROi | PR:Q63398 |
Gene expression databases
Bgeei | ENSRNOG00000028845, Expressed in spleen and 19 other tissues |
ExpressionAtlasi | Q63398, baseline and differential |
Genevisiblei | Q63398, RN |
Family and domain databases
CDDi | cd11606, COE_DBD, 1 hit cd01175, IPT_COE, 1 hit |
Gene3Di | 2.60.40.10, 1 hit 2.60.40.3180, 1 hit 4.10.280.10, 2 hits |
InterProi | View protein in InterPro IPR032200, COE_DBD IPR038173, COE_DBD_sf IPR032201, COE_HLH IPR038006, COE_IPT IPR036638, HLH_DNA-bd_sf IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR002909, IPT_dom IPR003523, Transcription_factor_COE IPR018350, Transcription_factor_COE_CS |
PANTHERi | PTHR10747, PTHR10747, 1 hit |
Pfami | View protein in Pfam PF16422, COE1_DBD, 1 hit PF16423, COE1_HLH, 1 hit PF01833, TIG, 1 hit |
SMARTi | View protein in SMART SM00429, IPT, 1 hit |
SUPFAMi | SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS01345, COE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | COE1_RAT | |
Accessioni | Q63398Primary (citable) accession number: Q63398 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | May 30, 2000 | |
Last modified: | April 7, 2021 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families