UniProtKB - Q63379 (MYCN_RAT)
N-myc proto-oncogene protein
Mycn
Functioni
Positively regulates the transcription of MYCNOS in neuroblastoma cells.
By similarityGO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: RGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- kinase binding Source: RGD
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: RGD
- sequence-specific double-stranded DNA binding Source: RGD
GO - Biological processi
- branching morphogenesis of an epithelial tube Source: RGD
- cartilage condensation Source: RGD
- embryonic digit morphogenesis Source: RGD
- embryonic skeletal system morphogenesis Source: RGD
- lung development Source: RGD
- negative regulation of astrocyte differentiation Source: RGD
- negative regulation of gene expression Source: RGD
- negative regulation of reactive oxygen species metabolic process Source: RGD
- positive regulation of cell death Source: RGD
- positive regulation of cell population proliferation Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of mesenchymal cell proliferation Source: RGD
- positive regulation of transcription, DNA-templated Source: RGD
- positive regulation of transcription by RNA polymerase II Source: RGD
- regulation of inner ear auditory receptor cell differentiation Source: RGD
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding |
Names & Taxonomyi
Protein namesi | Recommended name: N-myc proto-oncogene protein |
Gene namesi | Name:Mycn Synonyms:Nmyc, Nmyc1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1584995, Mycn |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127326 | 1 – 462 | N-myc proto-oncogene proteinAdd BLAST | 462 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 259 | Phosphoserine; by CK2By similarity | 1 | |
Modified residuei | 261 | Phosphoserine; by CK2By similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q63379 |
PTM databases
iPTMneti | Q63379 |
PhosphoSitePlusi | Q63379 |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX.
Interacts with KDM5A, KDM5B and HUWE1.
Interacts with MYCNOS.
Interacts with AURKA; interaction is phospho-independent and triggers AURKA activation; AURKA competes with FBXW7 for binding to unphosphorylated MYCN but not for binding to unphosphorylated MYCN.
Interacts with FBXW7; FBXW7 competes with AURKA for binding to unphosphorylated MYCN but not for binding to phosphorylated MYCN.
By similarityGO - Molecular functioni
- kinase binding Source: RGD
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000008643 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 379 – 431 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 19 – 47 | Interaction with AURKABy similarityAdd BLAST | 29 | |
Regioni | 61 – 90 | Interaction with AURKA and FBXW7By similarityAdd BLAST | 30 | |
Regioni | 133 – 177 | DisorderedSequence analysisAdd BLAST | 45 | |
Regioni | 232 – 290 | DisorderedSequence analysisAdd BLAST | 59 | |
Regioni | 332 – 390 | DisorderedSequence analysisAdd BLAST | 59 | |
Regioni | 431 – 452 | Leucine-zipperAdd BLAST | 22 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 76 – 85 | 9aaTADBy similarity | 10 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 257 – 276 | Acidic residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 370 – 390 | Basic and acidic residuesSequence analysisAdd BLAST | 21 |
Domaini
Phylogenomic databases
eggNOGi | KOG2588, Eukaryota |
InParanoidi | Q63379 |
OrthoDBi | 877891at2759 |
PhylomeDBi | Q63379 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR002418, Tscrpt_reg_Myc IPR012682, Tscrpt_reg_Myc_N |
Pfami | View protein in Pfam PF00010, HLH, 1 hit PF01056, Myc_N, 1 hit |
PIRSFi | PIRSF001705, Myc_protein, 1 hit |
PRINTSi | PR00044, LEUZIPPRMYC |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MPSCTASTMP GMICKNPDLE FDSLQPCFYP DEDDFYFGGP DSTPPGEDIW
60 70 80 90 100
KKFELLPTPP LSPSRAFPEH SPEPSNWATE MLLPEADLWG NPAEEDAFGL
110 120 130 140 150
GGLGGLTPNP VILQDCMWSG FSAREKLERA VNEKLQHGHG PPGASSSCPA
160 170 180 190 200
PGVGASSSGG RALGGSASAG RTGATLPTDL SHPAAECVDP AVVFPFPVNK
210 220 230 240 250
RESASVPAAP TSAPATSAVV TSVSVPAVAP VAAPARGSGR PANSGEHKAL
260 270 280 290 300
STSGEDTLSD SDDEDDEEED EEEEIDVVTV EKRRSSSNNK AVTTFTITVR
310 320 330 340 350
PKTSALGLGR AQPGELILKR CVPIHQQHNY AAPSPYVESE DAPPQKKIKS
360 370 380 390 400
EASPRPLKSV VPPKAKSLSP RNSDSEDSER RRNHNILERQ RRNDLRSSFL
410 420 430 440 450
TLRDHVPELV KNEKAAKVVI LKKATEYVHA LQANEHQLLL EKEKLQARQQ
460
QLLKKIEHAR TC
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V6T6 | G3V6T6_RAT | N-myc proto-oncogene protein | Mycn Mycn_mapped, rCG_62224 | 462 | Annotation score: | ||
A0A8I6AMA1 | A0A8I6AMA1_RAT | N-myc proto-oncogene protein | Mycn | 465 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X63281 Genomic DNA Translation: CAA44920.1 |
PIRi | S20073 |
RefSeqi | NP_001013114.1, NM_001013096.1 |
Genome annotation databases
GeneIDi | 298894 |
KEGGi | rno:298894 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X63281 Genomic DNA Translation: CAA44920.1 |
PIRi | S20073 |
RefSeqi | NP_001013114.1, NM_001013096.1 |
3D structure databases
AlphaFoldDBi | Q63379 |
SMRi | Q63379 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000008643 |
PTM databases
iPTMneti | Q63379 |
PhosphoSitePlusi | Q63379 |
Proteomic databases
PaxDbi | Q63379 |
Genome annotation databases
GeneIDi | 298894 |
KEGGi | rno:298894 |
Organism-specific databases
CTDi | 4613 |
RGDi | 1584995, Mycn |
Phylogenomic databases
eggNOGi | KOG2588, Eukaryota |
InParanoidi | Q63379 |
OrthoDBi | 877891at2759 |
PhylomeDBi | Q63379 |
Miscellaneous databases
PROi | PR:Q63379 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR002418, Tscrpt_reg_Myc IPR012682, Tscrpt_reg_Myc_N |
Pfami | View protein in Pfam PF00010, HLH, 1 hit PF01056, Myc_N, 1 hit |
PIRSFi | PIRSF001705, Myc_protein, 1 hit |
PRINTSi | PR00044, LEUZIPPRMYC |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MYCN_RAT | |
Accessioni | Q63379Primary (citable) accession number: Q63379 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |