UniProtKB - Q63364 (PJA2_RAT)
Protein
E3 ubiquitin-protein ligase Praja-2
Gene
Pja2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Has E2-dependent E3 ubiquitin-protein ligase activity. Responsible for ubiquitination of cAMP-dependent protein kinase type I and type II-alpha/beta regulatory subunits and for targeting them for proteasomal degradation. Essential for PKA-mediated long-term memory processes. Through the ubiquitination of MFHAS1, positively regulates the TLR2 signaling pathway that leads to the activation of the downstream p38 and JNK MAP kinases and promotes the polarization of macrophages toward the pro-inflammatory M1 phenotype.By similarity1 Publication
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 633 – 674 | RING-type; atypicalPROSITE-ProRule annotationAdd BLAST | 42 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- protein kinase A catalytic subunit binding Source: UniProtKB
- protein kinase A regulatory subunit binding Source: UniProtKB
- ubiquitin-protein transferase activity Source: RGD
GO - Biological processi
- hippo signaling Source: InterPro
- inflammatory response Source: UniProtKB
- innate immune response Source: UniProtKB
- long-term memory Source: UniProtKB
- positive regulation of JNK cascade Source: UniProtKB
- positive regulation of p38MAPK cascade Source: UniProtKB
- positive regulation of toll-like receptor 2 signaling pathway Source: UniProtKB
- protein ubiquitination Source: UniProtKB
- regulation of macrophage activation Source: RGD
- regulation of protein kinase A signaling Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase Praja-2 (EC:2.3.2.27)Short name: Praja2 Alternative name(s): RING-type E3 ubiquitin transferase Praja-2Curated |
Gene namesi | Name:Pja2 Synonyms:Neurodap1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620273, Pja2 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Peripheral membrane protein 1 Publication
Plasma membrane
- Cell membrane By similarity
Golgi apparatus
- Golgi apparatus membrane 1 Publication; Peripheral membrane protein 1 Publication
Other locations
- Cytoplasm 1 Publication
- synapse 1 Publication
- postsynaptic density 1 Publication
Note: Localizes at the cytoplasmic side of endoplasmic reticulum and Golgi apparatus. Expressed in the postsynaptic density region of synapses. Colocalizes with PRKAR2A and PRKAR2B in the cytoplasm and the cell membrane (By similarity).By similarity
Cytoskeleton
- intermediate filament cytoskeleton Source: Ensembl
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: RGD
Other locations
- cytoplasm Source: UniProtKB
- postsynaptic density Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000278233 | 2 – 707 | E3 ubiquitin-protein ligase Praja-2Add BLAST | 706 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 306 | PhosphoserineBy similarity | 1 | |
Modified residuei | 320 | PhosphoserineBy similarity | 1 | |
Modified residuei | 339 | Phosphoserine; by PKABy similarity | 1 | |
Modified residuei | 430 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q63364 |
PRIDEi | Q63364 |
PTM databases
iPTMneti | Q63364 |
PhosphoSitePlusi | Q63364 |
Expressioni
Tissue specificityi
Highly expressed in the brain, in nerve cells but not in glial cells. Abundantly expressed in pyramidal neurons and in the CA3 region of apical dendrites. Colocalizes with PRKAR2B in dentate granule cells and at postsynaptic sites of primary hippocampal neurons.2 Publications
Gene expression databases
Bgeei | ENSRNOG00000015528, Expressed in brain and 21 other tissues |
ExpressionAtlasi | Q63364, baseline and differential |
Genevisiblei | Q63364, RN |
Interactioni
Subunit structurei
Binds ubiquitin-conjugating enzymes (E2s). In vitro, interacts with the ubiquitin-conjugating enzyme, UBE2D2. The phosphorylated form interacts with PRKAR1A, PRKAR2A and PRKAR2B. Binds the catalytic subunits of cAMP-dependent protein kinase.
Interacts with MFHAS1.
By similarityGO - Molecular functioni
- protein kinase A catalytic subunit binding Source: UniProtKB
- protein kinase A regulatory subunit binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000021258 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 530 – 707 | Interaction with PRKAR1A, PRKAR2A and PRKAR2BBy similarityAdd BLAST | 178 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 633 – 674 | RING-type; atypicalPROSITE-ProRule annotationAdd BLAST | 42 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0800, Eukaryota |
GeneTreei | ENSGT00940000154585 |
InParanoidi | Q63364 |
OMAi | QNTSDRD |
OrthoDBi | 1249953at2759 |
PhylomeDBi | Q63364 |
TreeFami | TF330711 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR030639, Praja-2 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR15710:SF5, PTHR15710:SF5, 1 hit |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q63364-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQYTEKEPS VMDQDSSKAA WPRAAGGYQT ITGRRYGRRH AYVSFKPCMT
60 70 80 90 100
RHERSLGRAG DDYEVLELDD VAKENTAGSS SLDQVHPSLP SETTVEKSET
110 120 130 140 150
EIPTCGPALN QSTESNPSVA TVCHSEEVRE TLDSSTNLQN HAERECTPAV
160 170 180 190 200
CNASSVQNGI VLVHTDSYDP DSKHDENDSL QLCAQAVEGG RRQKVLGNAV
210 220 230 240 250
FELENGEVER YADLCPSVPS LSGEIREESE ELGSALLEKN SAGDAEAVHQ
260 270 280 290 300
DGQEFQRSSE DGIVRKRRQD DTDQGRQTEN STEDADCVPG HVEQNTSERA
310 320 330 340 350
NHHGSSPEQV VRPKVRKVIS SSQVDQESGF NRHEAKQRSV QRWREALEVE
360 370 380 390 400
ECSSDDPIIK CDDYDGDHDC MFLTPSYSRV TPREAERHRA TAENGATASG
410 420 430 440 450
RQEARENAFW NACGEYYQLF DKDEDSSECS DGEWSASLPH RFSGTEKDQS
460 470 480 490 500
SSDESWETLP GKDENEPELQ SDSSGPEEEN QELSLQEGEQ TSLEEGEIPW
510 520 530 540 550
LQYNEVNESS SDEGNEPANE FAQPEAFMLD GNNNLEDDSS VSEDLDVDWS
560 570 580 590 600
LFDGFADGLG VAEAISYVDP QFLTYMALEE RLAQAMETAL AHLESLAVDV
610 620 630 640 650
EVANPPASKE SIDGLPETLV LEDHTAIGQE QCCPICCSEY IKDDIATELP
660 670 680 690 700
CHHFFHKPCV SIWLQKSGTC PVCRRHFPPA VIDASAAASS EPDLDASPAN
DNAEEAP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0H2UHM9 | A0A0H2UHM9_RAT | E3 ubiquitin-protein ligase Praja-2 | Pja2 rCG_55395 | 645 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D32249 mRNA Translation: BAA06979.1 BC074015 mRNA Translation: AAH74015.1 |
RefSeqi | NP_001264207.1, NM_001277278.1 NP_620251.1, NM_138896.2 XP_017451755.1, XM_017596266.1 |
Genome annotation databases
Ensembli | ENSRNOT00000080962; ENSRNOP00000072362; ENSRNOG00000015528 |
GeneIDi | 192256 |
KEGGi | rno:192256 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D32249 mRNA Translation: BAA06979.1 BC074015 mRNA Translation: AAH74015.1 |
RefSeqi | NP_001264207.1, NM_001277278.1 NP_620251.1, NM_138896.2 XP_017451755.1, XM_017596266.1 |
3D structure databases
SMRi | Q63364 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000021258 |
PTM databases
iPTMneti | Q63364 |
PhosphoSitePlusi | Q63364 |
Proteomic databases
PaxDbi | Q63364 |
PRIDEi | Q63364 |
Genome annotation databases
Ensembli | ENSRNOT00000080962; ENSRNOP00000072362; ENSRNOG00000015528 |
GeneIDi | 192256 |
KEGGi | rno:192256 |
Organism-specific databases
CTDi | 9867 |
RGDi | 620273, Pja2 |
Phylogenomic databases
eggNOGi | KOG0800, Eukaryota |
GeneTreei | ENSGT00940000154585 |
InParanoidi | Q63364 |
OMAi | QNTSDRD |
OrthoDBi | 1249953at2759 |
PhylomeDBi | Q63364 |
TreeFami | TF330711 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-RNO-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
PROi | PR:Q63364 |
Gene expression databases
Bgeei | ENSRNOG00000015528, Expressed in brain and 21 other tissues |
ExpressionAtlasi | Q63364, baseline and differential |
Genevisiblei | Q63364, RN |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR030639, Praja-2 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR15710:SF5, PTHR15710:SF5, 1 hit |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PJA2_RAT | |
Accessioni | Q63364Primary (citable) accession number: Q63364 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 20, 2007 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 160 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways