UniProtKB - Q63273 (GRIK5_RAT)
Protein
Glutamate receptor ionotropic, kainate 5
Gene
Grik5
Organism
Rattus norvegicus (Rat)
Status
Functioni
Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds kainate > quisqualate > glutamate >> AMPA.
GO - Molecular functioni
- glutamate receptor activity Source: UniProtKB
- identical protein binding Source: UniProtKB
- kainate selective glutamate receptor activity Source: RGD
- ligand-gated ion channel activity Source: GO_Central
- PDZ domain binding Source: UniProtKB
- SH3 domain binding Source: UniProtKB
- signaling receptor activity Source: GO_Central
- transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential Source: RGD
GO - Biological processi
- cellular response to glucose stimulus Source: RGD
- chemical synaptic transmission Source: RGD
- establishment of localization in cell Source: UniProtKB
- excitatory postsynaptic potential Source: RGD
- modulation of chemical synaptic transmission Source: GO_Central
- positive regulation of neuron apoptotic process Source: RGD
- protein retention in ER lumen Source: UniProtKB
- receptor clustering Source: UniProtKB
- regulation of membrane potential Source: RGD
- regulation of synaptic vesicle fusion to presynaptic active zone membrane Source: RGD
- synaptic transmission, glutamatergic Source: RGD
Keywordsi
Molecular function | Ion channel, Ligand-gated ion channel, Receptor |
Biological process | Ion transport, Transport |
Enzyme and pathway databases
Reactomei | R-RNO-451308, Activation of Ca-permeable Kainate Receptor |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamate receptor ionotropic, kainate 5Short name: GluK5 Alternative name(s): Glutamate receptor KA-2 Short name: KA2 |
Gene namesi | Name:Grik5 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2735, Grik5 |
Subcellular locationi
Endoplasmic reticulum
- endoplasmic reticulum Source: RGD
Nucleus
- nucleoplasm Source: Ensembl
Plasma Membrane
- integral component of postsynaptic density membrane Source: RGD
- integral component of presynaptic membrane Source: SynGO
- ionotropic glutamate receptor complex Source: UniProtKB
- kainate selective glutamate receptor complex Source: RGD
- plasma membrane Source: GO_Central
- postsynaptic membrane Source: RGD
- presynaptic membrane Source: RGD
Other locations
- axon Source: RGD
- dendrite Source: UniProtKB
- glutamatergic synapse Source: SynGO
- hippocampal mossy fiber to CA3 synapse Source: RGD
- membrane Source: RGD
- neuron projection Source: RGD
- neuronal cell body Source: RGD
- perikaryon Source: RGD
- postsynaptic density Source: RGD
- terminal bouton Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 15 – 544 | ExtracellularSequence analysisAdd BLAST | 530 | |
Transmembranei | 545 – 565 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 566 – 622 | CytoplasmicSequence analysisAdd BLAST | 57 | |
Transmembranei | 623 – 643 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 644 – 803 | ExtracellularSequence analysisAdd BLAST | 160 | |
Transmembranei | 804 – 824 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 825 – 979 | CytoplasmicSequence analysisAdd BLAST | 155 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 14 | Sequence analysisAdd BLAST | 14 | |
ChainiPRO_0000011554 | 15 – 979 | Glutamate receptor ionotropic, kainate 5Add BLAST | 965 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 36 ↔ 292 | |||
Disulfide bondi | 83 ↔ 334 | |||
Disulfide bondi | 165 ↔ 170 | |||
Glycosylationi | 219 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 271 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 285 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 322 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 372 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 394 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 400 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 407 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 414 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 478 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 735 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q63273 |
PRIDEi | Q63273 |
PTM databases
GlyGeni | Q63273, 10 sites |
iPTMneti | Q63273 |
PhosphoSitePlusi | Q63273 |
Expressioni
Tissue specificityi
High expression in the cerebral cortex, pyriform cortex, caudate-putamen, hippocampal complex, medial habenulata and granule cell layer of the cerebellum. Weak expression in globus pallidus, thalamus, colliculi and the reticular thalamic nucleus.
Developmental stagei
From embryonic day 14 through postnatal day 1, high expression in spinal cord, brain and some areas of the PNS. At E17 and E19, high expression in spinal cord, mesencephalon and telencephalic structures as in olfactory neurons and in nasal epithelium. Strong expression also in the pituitary.
Gene expression databases
Bgeei | ENSRNOG00000020310, Expressed in brain and 22 other tissues |
Genevisiblei | Q63273, RN |
Interactioni
Subunit structurei
Tetramer of two or more different subunits. Associates with GRIK1 (both edited and unedited versions), GRIK2, or GRIK3 to form functional channels. Homomeric associations do not produce any channel activity.
1 PublicationGO - Molecular functioni
- identical protein binding Source: UniProtKB
- PDZ domain binding Source: UniProtKB
- SH3 domain binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 246572, 1 interactor |
STRINGi | 10116.ENSRNOP00000027578 |
Chemistry databases
BindingDBi | Q63273 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q63273 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 860 – 866 | Poly-Arg | 7 |
Sequence similaritiesi
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK5 subfamily. [View classification]Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1052, Eukaryota |
GeneTreei | ENSGT00940000158852 |
HOGENOMi | CLU_007257_1_0_1 |
InParanoidi | Q63273 |
OMAi | CTSSNIW |
OrthoDBi | 188544at2759 |
PhylomeDBi | Q63273 |
TreeFami | TF334668 |
Family and domain databases
InterProi | View protein in InterPro IPR001828, ANF_lig-bd_rcpt IPR019594, Glu/Gly-bd IPR001508, Iono_rcpt_met IPR001320, Iontro_rcpt IPR028082, Peripla_BP_I |
Pfami | View protein in Pfam PF01094, ANF_receptor, 1 hit PF00060, Lig_chan, 1 hit PF10613, Lig_chan-Glu_bd, 1 hit |
PRINTSi | PR00177, NMDARECEPTOR |
SMARTi | View protein in SMART SM00918, Lig_chan-Glu_bd, 1 hit SM00079, PBPe, 1 hit |
SUPFAMi | SSF53822, SSF53822, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q63273-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPAELLLLLI VAFANPSCQV LSSLRMAAIL DDQTVCGRGE RLALALAREQ
60 70 80 90 100
INGIIEVPAK ARVEVDIFEL QRDSQYETTD TMCQILPKGV VSVLGPSSSP
110 120 130 140 150
ASASTVSHIC GEKEIPHIKV GPEETPRLQY LRFASVSLYP SNEDVSLAVS
160 170 180 190 200
RILKSFNYPS ASLICAKAEC LLRLEELVRG FLISKETLSV RMLDDSRDPT
210 220 230 240 250
PLLKEIRDDK VSTIIIDANA SISHLVLRKA SELGMTSAFY KYILTTMDFP
260 270 280 290 300
ILHLDGIVED SSNILGFSMF NTSHPFYPEF VRSLNMSWRE NCEASTYPGP
310 320 330 340 350
ALSAALMFDA VHVVVSAVRE LNRSQEIGVK PLACTSANIW PHGTSLMNYL
360 370 380 390 400
RMVEYDGLTG RVEFNSKGQR TNYTLRILEK SRQGHREIGV WYSNRTLAMN
410 420 430 440 450
ATTLDINLSQ TLANKTLVVT TILENPYVMR RPNFQALSGN ERFEGFCVDM
460 470 480 490 500
LRELAELLRF RYRLRLVEDG LYGAPEPNGS WTGMVGELIN RKADLAVAAF
510 520 530 540 550
TITAEREKVI DFSKPFMTLG ISILYRVHMG RKPGYFSFLD PFSPAVWLFM
560 570 580 590 600
LLAYLAVSCV LFLAARLSPY EWYNPHPCLR ARPHILENQY TLGNSLWFPV
610 620 630 640 650
GGFMQQGSEI MPRALSTRCV SGVWWAFTLI IISSYTANLA AFLTVQRMEV
660 670 680 690 700
PVESADDLAD QTNIEYGTIH AGSTMTFFQN SRYQTYQRMW NYMQSKQPSV
710 720 730 740 750
FVKSTEEGIA RVLNSRYAFL LESTMNEYHR RLNCNLTQIG GLLDTKGYGI
760 770 780 790 800
GMPLGSPFRD EITLAILQLQ ENNRLEILKR KWWEGGRCPK EEDHRAKGLG
810 820 830 840 850
MENIGGIFVV LICGLIIAVF VAVMEFIWST RRSAESEEVS VCQEMLQELR
860 870 880 890 900
HAVSCRKTSR SRRRRRPGGP SRALLSLRAV REMRLSNGKL YSAGAGGDAG
910 920 930 940 950
AHGGPQRLLD DPGPPGGPRP QAPTPCTHVR VCQECRRIQA LRASGAGAPP
960 970
RGLGTPAEAT SPPRPRPGPT GPRELTEHE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 887 | N → G (Ref. 2) Curated | 1 | |
Sequence conflicti | 889 | K → R (Ref. 2) Curated | 1 | |
Sequence conflicti | 910 | D → N in AAA17831 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11581 mRNA Translation: CAA77667.1 U08258 mRNA Translation: AAA17831.1 |
PIRi | JH0592 |
RefSeqi | NP_113696.1, NM_031508.2 |
Genome annotation databases
Ensembli | ENSRNOT00000027578; ENSRNOP00000027578; ENSRNOG00000020310 |
GeneIDi | 24407 |
KEGGi | rno:24407 |
UCSCi | RGD:2735, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11581 mRNA Translation: CAA77667.1 U08258 mRNA Translation: AAA17831.1 |
PIRi | JH0592 |
RefSeqi | NP_113696.1, NM_031508.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3OM0 | X-ray | 1.40 | A | 20-406 | [»] | |
3OM1 | X-ray | 1.68 | A/B | 20-406 | [»] | |
3QLU | X-ray | 2.91 | A/B | 20-406 | [»] | |
3QLV | X-ray | 3.94 | A/B/E/G/I | 20-406 | [»] | |
SMRi | Q63273 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 246572, 1 interactor |
STRINGi | 10116.ENSRNOP00000027578 |
Chemistry databases
BindingDBi | Q63273 |
ChEMBLi | CHEMBL4041 |
DrugCentrali | Q63273 |
PTM databases
GlyGeni | Q63273, 10 sites |
iPTMneti | Q63273 |
PhosphoSitePlusi | Q63273 |
Proteomic databases
PaxDbi | Q63273 |
PRIDEi | Q63273 |
Protocols and materials databases
ABCDi | Q63273, 1 sequenced antibody |
Genome annotation databases
Ensembli | ENSRNOT00000027578; ENSRNOP00000027578; ENSRNOG00000020310 |
GeneIDi | 24407 |
KEGGi | rno:24407 |
UCSCi | RGD:2735, rat |
Organism-specific databases
CTDi | 2901 |
RGDi | 2735, Grik5 |
Phylogenomic databases
eggNOGi | KOG1052, Eukaryota |
GeneTreei | ENSGT00940000158852 |
HOGENOMi | CLU_007257_1_0_1 |
InParanoidi | Q63273 |
OMAi | CTSSNIW |
OrthoDBi | 188544at2759 |
PhylomeDBi | Q63273 |
TreeFami | TF334668 |
Enzyme and pathway databases
Reactomei | R-RNO-451308, Activation of Ca-permeable Kainate Receptor |
Miscellaneous databases
PROi | PR:Q63273 |
Gene expression databases
Bgeei | ENSRNOG00000020310, Expressed in brain and 22 other tissues |
Genevisiblei | Q63273, RN |
Family and domain databases
InterProi | View protein in InterPro IPR001828, ANF_lig-bd_rcpt IPR019594, Glu/Gly-bd IPR001508, Iono_rcpt_met IPR001320, Iontro_rcpt IPR028082, Peripla_BP_I |
Pfami | View protein in Pfam PF01094, ANF_receptor, 1 hit PF00060, Lig_chan, 1 hit PF10613, Lig_chan-Glu_bd, 1 hit |
PRINTSi | PR00177, NMDARECEPTOR |
SMARTi | View protein in SMART SM00918, Lig_chan-Glu_bd, 1 hit SM00079, PBPe, 1 hit |
SUPFAMi | SSF53822, SSF53822, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GRIK5_RAT | |
Accessioni | Q63273Primary (citable) accession number: Q63273 Secondary accession number(s): Q62643 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families