UniProtKB - Q63259 (PTPRN_RAT)
Receptor-type tyrosine-protein phosphatase-like N
Ptprn
Functioni
Caution
GO - Molecular functioni
- GTPase binding Source: RGD
- spectrin binding Source: RGD
- transcription factor binding Source: RGD
- ubiquitin-like protein ligase binding Source: RGD
GO - Biological processi
- cytokine-mediated signaling pathway Source: RGD
- dense core granule maturation Source: RGD
- insulin secretion Source: RGD
- insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
- luteinization Source: UniProtKB
- peptidyl-tyrosine dephosphorylation Source: GOC
- positive regulation of transcription by RNA polymerase II Source: RGD
- positive regulation of type B pancreatic cell proliferation Source: UniProtKB
- response to cAMP Source: RGD
- response to estrogen Source: RGD
- response to glucose Source: RGD
- response to insulin Source: RGD
- response to reactive oxygen species Source: UniProtKB
- second-messenger-mediated signaling Source: RGD
Keywordsi
Molecular function | Receptor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Receptor-type tyrosine-protein phosphatase-like NShort name: R-PTP-N Alternative name(s): 105 kDa islet cell antigen BEM-3 Brain-enriched membrane-associated protein tyrosine phosphatase ICA1051 Publication ICA5121 Publication PTP IA-21 Publication PTPLP Cleaved into the following 3 chains: |
Gene namesi | Name:Ptprn |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620777, Ptprn |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Single-pass type I membrane protein Curated
Endosome
- Endosome 1 Publication
Other locations
- Membrane 2 Publications; Single-pass type I membrane protein Curated
- secretory vesicle membrane 2 Publications; Single-pass type I membrane protein Curated
- Perikaryon 1 Publication
- axon 1 Publication
- synapse 1 Publication
Note: Detected on neuronal secretory vesicles, but not on synaptic vesicles. Colocalizes with insulin-containing secretory granules. Primarily detected on secretory vesicle membranes. Transiently found at the cell membrane, when secretory vesicles fuse with the cell membrane to release their cargo. Is then endocytosed and recycled to secretory vesicles via the Golgi apparatus membranes.1 Publication
Other locations
- secretory vesicle membrane 2 Publications
Nucleus
- Nucleus By similarity
Endosome
- endosome Source: UniProtKB-SubCell
Nucleus
- nucleus Source: RGD
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- axon terminus Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
- membrane Source: RGD
- neuronal cell body Source: UniProtKB
- perikaryon Source: UniProtKB-SubCell
- secretory granule Source: UniProtKB
- synapse Source: UniProtKB
- transport vesicle membrane Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 41 – 579 | LumenalSequence analysisAdd BLAST | 539 | |
Transmembranei | 580 – 604 | HelicalSequence analysisAdd BLAST | 25 | |
Topological domaini | 605 – 983 | CytoplasmicSequence analysisAdd BLAST | 379 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasmic vesicle, Endosome, Membrane, Nucleus, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 40 | By similarityAdd BLAST | 40 | |
ChainiPRO_0000025453 | 41 – 983 | Receptor-type tyrosine-protein phosphatase-like NAdd BLAST | 943 | |
ChainiPRO_0000438085 | 41 – 452 | ICA512-N-terminal fragmentBy similarityAdd BLAST | 412 | |
ChainiPRO_0000438086 | 453 – 983 | ICA512-transmembrane fragmentBy similarityAdd BLAST | 531 | |
ChainiPRO_0000438087 | 663 – 983 | ICA512-cleaved cytosolic fragmentBy similarityAdd BLAST | 321 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 46 | Interchain (with C-46 or C-53); in multimeric formBy similarity | ||
Disulfide bondi | 53 | Interchain (with C-46 or C-53); in multimeric formBy similarity | ||
Disulfide bondi | 59 ↔ 68 | By similarity | ||
Modified residuei | 311 | PhosphoserineCombined sources | 1 | |
Modified residuei | 312 | PhosphoserineCombined sources | 1 | |
Glycosylationi | 510 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 528 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Cross-linki | 758 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 452 – 453 | CleavageBy similarity | 2 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q63259 |
PaxDbi | Q63259 |
PRIDEi | Q63259 |
PTM databases
GlyGeni | Q63259, 2 sites |
iPTMneti | Q63259 |
PhosphoSitePlusi | Q63259 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000019587, Expressed in frontal cortex and 20 other tissues |
Genevisiblei | Q63259, RN |
Interactioni
Subunit structurei
Homodimer; shown for the unprocessed protein (proICA512) in the endoplasmic reticulum and resolved during protein maturation as ICA512-TMF seems to be predominantly monomeric in secretory granules; however, ICA512-CCF interacts with ICA512-TMF disrupting the ICA512-TMF:SNTB2 complex. The isolated lumenal RESP18 homology domain has been shown to form disulfide-linked homooligomers.
Interacts (via cytoplasmic domain) with phosphorylated SNTB2; this protects PTPRN against cleavage by CAPN1 to produce ICA512-CCF. Dephosphorylation of SNTB2 upon insulin stimulation disrupts the interaction and results in PTPRN cleavage.
Interacts with SNX19. ICA512-CCF interacts with PIAS4; in the nucleus.
Interacts with STAT5B (phosphorylated); down-regulated by ICA512-CCF sumoylation; ICA512-CCF prevents STAT5B dephosphorylation; ICA512-CCF mediates interaction of STAT5B with PIAS4.
Interacts (via RESP18 homology domain) with insulin and proinsulin.
Interacts with PTPRN2, PTPRA and PTPRE.
By similarityGO - Molecular functioni
- GTPase binding Source: RGD
- spectrin binding Source: RGD
- transcription factor binding Source: RGD
- ubiquitin-like protein ligase binding Source: RGD
Protein-protein interaction databases
IntActi | Q63259, 2 interactors |
STRINGi | 10116.ENSRNOP00000026654 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 713 – 973 | Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST | 261 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 41 – 137 | RESP18 homology domainBy similarityAdd BLAST | 97 | |
Regioni | 453 – 579 | Sufficient for dimerization of proICA512By similarityAdd BLAST | 127 | |
Regioni | 605 – 736 | Sufficient for dimerization of proICA512By similarityAdd BLAST | 132 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0793, Eukaryota |
GeneTreei | ENSGT00940000154095 |
HOGENOMi | CLU_007905_2_0_1 |
InParanoidi | Q63259 |
OMAi | ALCMRQH |
OrthoDBi | 411281at2759 |
PhylomeDBi | Q63259 |
TreeFami | TF351976 |
Family and domain databases
Gene3Di | 3.30.70.2470, 1 hit 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR033522, IA-2/IA-2_beta IPR029021, Prot-tyrosine_phosphatase-like IPR000242, PTPase_domain IPR021613, Receptor_IA-2_dom IPR038112, Receptor_IA-2_ectodomain_sf IPR029403, RESP18_dom IPR016130, Tyr_Pase_AS IPR003595, Tyr_Pase_cat IPR000387, TYR_PHOSPHATASE_dom |
PANTHERi | PTHR46106, PTHR46106, 1 hit |
Pfami | View protein in Pfam PF11548, Receptor_IA-2, 1 hit PF14948, RESP18, 1 hit PF00102, Y_phosphatase, 1 hit |
PRINTSi | PR00700, PRTYPHPHTASE |
SMARTi | View protein in SMART SM00194, PTPc, 1 hit SM00404, PTPc_motif, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit |
PROSITEi | View protein in PROSITE PS00383, TYR_PHOSPHATASE_1, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50055, TYR_PHOSPHATASE_PTP, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MRRPRRPGGP AGCGGSEGSG GLRLLVCLLL LSGRPGGCSA ISAHGCLFDR
60 70 80 90 100
RLCSHLEVCI QDGLFGQCQA GVGQARPLLQ VTSPVLQRLQ GVLRQLMSQG
110 120 130 140 150
LSWHDDLTQY VISQEMERIP RLRPPEPHPR DRSGSVPRRA GPAGELLSQG
160 170 180 190 200
NPTGSSPAVQ GLSRPPGDGN GAGVGSPLSS LQAELLPPLL EHLLMPPQPP
210 220 230 240 250
HPSLTYEPAL LQPYLFQQFG SRDGSRGSES ASGVVGHLAK AEDPVLFSRS
260 270 280 290 300
LSKAILGTHS GHSFGDLTGP SPAQLFQDSG LLYMAQELPV PGRARAPRLP
310 320 330 340 350
EEGGSSRAED SSEGHEEEVL GGHGEKSPPQ AVQADVSLQR LAAVLAGYGV
360 370 380 390 400
ELRQLTPEQL STLLTLLQLL PKGTGRHLGG AVNGGADVKK TIEEQMQRGD
410 420 430 440 450
TADARPPTPL LPGHPTASST SIKVRQVLSP GFPEPPKTSS PLGISAVLLE
460 470 480 490 500
KKSPLGQSQP TVVGQPSARP SAEEYGYIVT DQKPLSLVAG VKLLEILAEH
510 520 530 540 550
VHMTSGSFIN ISVVGPAVTF RIRHNEQNLS LADVTQQAGL VKSELEAQTG
560 570 580 590 600
LQILQTGVGQ REESAAVLPR QAHGISPMRS LLLTLVALAG VAGLLVALAV
610 620 630 640 650
ALCMRHHSKQ RDKERLAALG PEGAHGDTTF EYQDLCRQHM ATKSLFNRAE
660 670 680 690 700
GQPEPSRVSS VSSQFSDAAQ ASPSSHSSTP SWCEEPAQAN MDISTGHMIL
710 720 730 740 750
AYMEDHLRNR DRLAKEWQAL CAYQAEPNTC ATAQGEGNIK KNRHPDFLPY
760 770 780 790 800
DHARIKLKVE SSPSRSDYIN ASPIIEHDPR MPAYIATQGP LSHTIADFWQ
810 820 830 840 850
MVWESGCTVI VMLTPLVEDG VKQCDRYWPD EGSSLYHVYE VNLVSEHIWC
860 870 880 890 900
EDFLVRSFYL KNVQTQETRT LTQFHFLSWP AEGTPASTRP LLDFRRKVNK
910 920 930 940 950
CYRGRSCPII VHCSDGAGRT GTYILIDMVL NRMAKGVKEI DIAATLEHVR
960 970 980
DQRPGLVRSK DQFEFALTAV AEEVNAILKA LPQ
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 466 | P → T in AAA83235 (PubMed:7657822).Curated | 1 | |
Sequence conflicti | 507 | S → R in AAA83235 (PubMed:7657822).Curated | 1 | |
Sequence conflicti | 517 | A → V in AAA83235 (PubMed:7657822).Curated | 1 | |
Sequence conflicti | 520 | F → S in AAA83235 (PubMed:7657822).Curated | 1 | |
Sequence conflicti | 564 | S → A in AAA83235 (PubMed:7657822).Curated | 1 | |
Sequence conflicti | 566 | A → E in AAA83235 (PubMed:7657822).Curated | 1 | |
Sequence conflicti | 574 | G → R in BAA07397 (PubMed:7887886).Curated | 1 | |
Sequence conflicti | 581 | L → V in AAA83235 (PubMed:7657822).Curated | 1 | |
Sequence conflicti | 599 | A → V in BAA08254 (Ref. 4) Curated | 1 | |
Sequence conflicti | 609 | K → R in AAA83235 (PubMed:7657822).Curated | 1 | |
Sequence conflicti | 664 | Q → K in AAA83235 (PubMed:7657822).Curated | 1 | |
Sequence conflicti | 672 | S → N in AAA83235 (PubMed:7657822).Curated | 1 | |
Sequence conflicti | 714 | A → P in AAA83235 (PubMed:7657822).Curated | 1 | |
Sequence conflicti | 716 | E → K in CAA63313 (PubMed:7568143).Curated | 1 | |
Sequence conflicti | 731 | A → S in AAA83235 (PubMed:7657822).Curated | 1 | |
Sequence conflicti | 953 – 955 | RPG → PTC in AAA83235 (PubMed:7657822).Curated | 3 | |
Sequence conflicti | 962 | Q → K in AAA83235 (PubMed:7657822).Curated | 1 | |
Sequence conflicti | 966 | A → P in AAA83235 (PubMed:7657822).Curated | 1 | |
Sequence conflicti | 969 | A → P in AAA83235 (PubMed:7657822).Curated | 1 | |
Sequence conflicti | 971 | A → G in AAA83235 (PubMed:7657822).Curated | 1 | |
Sequence conflicti | 976 | A → P in AAA83235 (PubMed:7657822).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X92563 mRNA Translation: CAA63313.1 D38222 mRNA Translation: BAA07397.1 Frameshift. U40652 mRNA Translation: AAA83235.1 D45414 mRNA Translation: BAA08254.1 |
PIRi | S54342 |
RefSeqi | NP_446333.1, NM_053881.1 |
Genome annotation databases
Ensembli | ENSRNOT00000026654; ENSRNOP00000026654; ENSRNOG00000019587 |
GeneIDi | 116660 |
KEGGi | rno:116660 |
UCSCi | RGD:620777, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X92563 mRNA Translation: CAA63313.1 D38222 mRNA Translation: BAA07397.1 Frameshift. U40652 mRNA Translation: AAA83235.1 D45414 mRNA Translation: BAA08254.1 |
PIRi | S54342 |
RefSeqi | NP_446333.1, NM_053881.1 |
3D structure databases
SMRi | Q63259 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q63259, 2 interactors |
STRINGi | 10116.ENSRNOP00000026654 |
PTM databases
GlyGeni | Q63259, 2 sites |
iPTMneti | Q63259 |
PhosphoSitePlusi | Q63259 |
Proteomic databases
jPOSTi | Q63259 |
PaxDbi | Q63259 |
PRIDEi | Q63259 |
Genome annotation databases
Ensembli | ENSRNOT00000026654; ENSRNOP00000026654; ENSRNOG00000019587 |
GeneIDi | 116660 |
KEGGi | rno:116660 |
UCSCi | RGD:620777, rat |
Organism-specific databases
CTDi | 5798 |
RGDi | 620777, Ptprn |
Phylogenomic databases
eggNOGi | KOG0793, Eukaryota |
GeneTreei | ENSGT00940000154095 |
HOGENOMi | CLU_007905_2_0_1 |
InParanoidi | Q63259 |
OMAi | ALCMRQH |
OrthoDBi | 411281at2759 |
PhylomeDBi | Q63259 |
TreeFami | TF351976 |
Miscellaneous databases
PROi | PR:Q63259 |
Gene expression databases
Bgeei | ENSRNOG00000019587, Expressed in frontal cortex and 20 other tissues |
Genevisiblei | Q63259, RN |
Family and domain databases
Gene3Di | 3.30.70.2470, 1 hit 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR033522, IA-2/IA-2_beta IPR029021, Prot-tyrosine_phosphatase-like IPR000242, PTPase_domain IPR021613, Receptor_IA-2_dom IPR038112, Receptor_IA-2_ectodomain_sf IPR029403, RESP18_dom IPR016130, Tyr_Pase_AS IPR003595, Tyr_Pase_cat IPR000387, TYR_PHOSPHATASE_dom |
PANTHERi | PTHR46106, PTHR46106, 1 hit |
Pfami | View protein in Pfam PF11548, Receptor_IA-2, 1 hit PF14948, RESP18, 1 hit PF00102, Y_phosphatase, 1 hit |
PRINTSi | PR00700, PRTYPHPHTASE |
SMARTi | View protein in SMART SM00194, PTPc, 1 hit SM00404, PTPc_motif, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit |
PROSITEi | View protein in PROSITE PS00383, TYR_PHOSPHATASE_1, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50055, TYR_PHOSPHATASE_PTP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PTPRN_RAT | |
Accessioni | Q63259Primary (citable) accession number: Q63259 Secondary accession number(s): Q62883, Q63795, Q64643 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 15, 1998 | |
Last modified: | December 2, 2020 | |
This is version 150 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families