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Protein

Disintegrin and metalloproteinase domain-containing protein 2

Gene

Adam2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • male gonad development Source: RGD
  • single fertilization Source: InterPro
  • spermatogenesis Source: RGD

Keywordsi

Biological processCell adhesion

Protein family/group databases

MEROPSiM12.950

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 2
Short name:
ADAM 2
Alternative name(s):
Fertilin subunit beta
PH-30
Short name:
PH30
PH30-beta
Gene namesi
Name:Adam2
Synonyms:Ftnb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69299 Adam2

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 688ExtracellularSequence analysisAdd BLAST670
Transmembranei689 – 709HelicalSequence analysisAdd BLAST21
Topological domaini710 – 737CytoplasmicSequence analysisAdd BLAST28

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
PropeptideiPRO_000002905019 – 180By similarityAdd BLAST162
ChainiPRO_0000029051181 – 737Disintegrin and metalloproteinase domain-containing protein 2Add BLAST557

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi128N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi226N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi293 ↔ 376By similarity
Disulfide bondi335 ↔ 360By similarity
Disulfide bondi337 ↔ 342By similarity
Glycosylationi359N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi450 ↔ 470By similarity
Glycosylationi464N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi491N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi571N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi621 ↔ 632By similarity
Disulfide bondi626 ↔ 638By similarity
Disulfide bondi640 ↔ 649By similarity
Modified residuei731PhosphoserineCombined sources1

Post-translational modificationi

The prodomain and the metalloprotease domain are cleaved during the epididymal maturation of the spermatozoa.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ63202
PRIDEiQ63202

PTM databases

iPTMnetiQ63202
PhosphoSitePlusiQ63202

Interactioni

Subunit structurei

Heterodimer with ADAM1/fertilin subunit alpha.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000061122

Structurei

3D structure databases

ProteinModelPortaliQ63202
SMRiQ63202
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini184 – 381Peptidase M12BPROSITE-ProRule annotationAdd BLAST198
Domaini389 – 478DisintegrinPROSITE-ProRule annotationAdd BLAST90
Domaini617 – 650EGF-likePROSITE-ProRule annotationAdd BLAST34

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi482 – 616Cys-richAdd BLAST135

Domaini

A tripeptide motif (NQE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.By similarity

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3607 Eukaryota
ENOG410XX2M LUCA
HOGENOMiHOG000230883
HOVERGENiHBG103628
InParanoidiQ63202
KOiK06833
PhylomeDBiQ63202

Family and domain databases

CDDicd04269 ZnMc_adamalysin_II_like, 1 hit
Gene3Di3.40.390.10, 1 hit
4.10.70.10, 1 hit
InterProiView protein in InterPro
IPR033958 ADAM2
IPR006586 ADAM_Cys-rich
IPR018358 Disintegrin_CS
IPR001762 Disintegrin_dom
IPR036436 Disintegrin_dom_sf
IPR000742 EGF-like_dom
IPR024079 MetalloPept_cat_dom_sf
IPR001590 Peptidase_M12B
IPR002870 Peptidase_M12B_N
IPR034027 Reprolysin_adamalysin
PANTHERiPTHR11905:SF108 PTHR11905:SF108, 1 hit
PfamiView protein in Pfam
PF08516 ADAM_CR, 1 hit
PF00200 Disintegrin, 1 hit
PF01562 Pep_M12B_propep, 1 hit
PF01421 Reprolysin, 1 hit
SMARTiView protein in SMART
SM00608 ACR, 1 hit
SM00050 DISIN, 1 hit
SUPFAMiSSF57552 SSF57552, 1 hit
PROSITEiView protein in PROSITE
PS50215 ADAM_MEPRO, 1 hit
PS00427 DISINTEGRIN_1, 1 hit
PS50214 DISINTEGRIN_2, 1 hit
PS50026 EGF_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q63202-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLLLLLLSG LSRLGGLSEP QTEGTREKLH VQVTVPEKIR SITSEGYETQ
60 70 80 90 100
VTYSLKIEGK TYILNLMQKA FLPPNFRVYS YDSTGIMRPL EQKFQNICYF
110 120 130 140 150
QGYIEGYPNS MVIVSTCTGL RGVLQFGNVS YGIEPLESSS GFEHVIYQVE
160 170 180 190 200
PKKGDTLLYA EKDMDLRDPQ YKIRSIKPQR TVSHYLEIHI VVEKQMFEHI
210 220 230 240 250
GADTAVVTQK IFQLIGLTNA IFAPFNLTVI LSSLEFWMDE NKISTTGDAN
260 270 280 290 300
KLLYRFLKWK QSYLVLRPHD MAFLLVYRDT TDYVGATYQG KMCDKNYAGG
310 320 330 340 350
VALHPKAVTL ESLAIILVQL LSLSMGVAYD DVNTCQCGVP ICVMNPEALH
360 370 380 390 400
SSGVRSFSNC SMEDFSKFIV SQSSHCLQNQ PHLQPSYKMA VCGNGELEEG
410 420 430 440 450
EVCDCGQEGC DDKPPPCCNP TTCQLSEGST CSTGSCCDAS CNLKAKGELC
460 470 480 490 500
RPANQECDVT EYCNGTSEVC EEDFFVQDGH PCAEQKWICI NGTCQSGAQQ
510 520 530 540 550
CRDLFGTDAD YGTKECYSEL NSKSDISGSC GITPTGYKDC APNDRMCGKL
560 570 580 590 600
ICIYQSEDIL KMRSAIVIYA NISGQICISL EYPPGHKESK KMCVRDGTVC
610 620 630 640 650
GSGKVCLNQE CVEDTFLNYD CTPEKCNHHG VCNNKKHCHC EPTYLPPDCK
660 670 680 690 700
NTEDTWPGGS VDSGNQQRAE SIPARSYVAS AYRSKSARWP FFLIIPFYVV
710 720 730
ILVLIGMLVK VYSQRKKWRM DDFSSEEQFE SESESKD
Length:737
Mass (Da):82,311
Last modified:February 1, 2003 - v2
Checksum:i647E92A90D7CC1D1
GO

Sequence cautioni

The sequence CAA68127 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99794 mRNA Translation: CAA68127.1 Different initiation.
RefSeqiNP_064462.1, NM_020077.1
UniGeneiRn.42917

Genome annotation databases

GeneIDi56806
KEGGirno:56806
UCSCiRGD:69299 rat

Similar proteinsi

Entry informationi

Entry nameiADAM2_RAT
AccessioniPrimary (citable) accession number: Q63202
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: February 1, 2003
Last modified: May 23, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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