UniProtKB - Q63042 (ALR_RAT)
Protein
FAD-linked sulfhydryl oxidase ALR
Gene
Gfer
Organism
Rattus norvegicus (Rat)
Status
Functioni
FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. May have a function in liver regeneration and spermatogenesis.1 Publication
Catalytic activityi
- EC:1.8.3.2
Cofactori
FADPROSITE-ProRule annotation1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 104 | FAD2 Publications | 1 | |
Binding sitei | 133 | FAD2 Publications | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 92 – 100 | FAD2 Publications | 9 | |
Nucleotide bindingi | 164 – 176 | FAD2 PublicationsAdd BLAST | 13 | |
Nucleotide bindingi | 187 – 188 | FAD2 Publications | 2 |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: UniProtKB
- flavin-linked sulfhydryl oxidase activity Source: RGD
- growth factor activity Source: UniProtKB-KW
- protein disulfide oxidoreductase activity Source: UniProtKB
GO - Biological processi
- cellular response to actinomycin D Source: RGD
- cellular response to lipopolysaccharide Source: RGD
- cellular response to toxic substance Source: RGD
- cellular response to tumor necrosis factor Source: RGD
- liver development Source: GO_Central
- liver regeneration Source: RGD
- negative regulation of apoptotic process Source: RGD
- negative regulation of natural killer cell mediated cytotoxicity Source: RGD
- negative regulation of oxidative stress-induced neuron death Source: RGD
- positive regulation of DNA biosynthetic process Source: RGD
- response to lipopolysaccharide Source: RGD
Keywordsi
Molecular function | Growth factor, Oxidoreductase |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
BRENDAi | 1.8.3.2, 5301 |
Names & Taxonomyi
Protein namesi | Recommended name: FAD-linked sulfhydryl oxidase ALR (EC:1.8.3.2)Alternative name(s): Augmenter of liver regeneration |
Gene namesi | Name:Gfer Synonyms:Alr |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61845, Gfer |
Subcellular locationi
Mitochondrion
- Mitochondrion intermembrane space By similarity
- Mitochondrion By similarity
Cytosol
- cytosol Source: RGD
Extracellular region or secreted
- extracellular space Source: RGD
Mitochondrion
- mitochondrial intermembrane space Source: UniProtKB-SubCell
- mitochondrion Source: RGD
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000208550 | 1 – 198 | FAD-linked sulfhydryl oxidase ALRAdd BLAST | 198 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 88 | Interchain (with C-197)PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 135 ↔ 138 | Redox-activePROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 164 ↔ 181 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 197 | Interchain (with C-88)PROSITE-ProRule annotation1 Publication |
Keywords - PTMi
Disulfide bondProteomic databases
PaxDbi | Q63042 |
PRIDEi | Q63042 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000013370, Expressed in testis and 22 other tissues |
Genevisiblei | Q63042, RN |
Interactioni
Subunit structurei
GO - Molecular functioni
- growth factor activity Source: UniProtKB-KW
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000017917 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q63042 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q63042 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 88 – 188 | ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd BLAST | 101 |
Phylogenomic databases
eggNOGi | KOG3355, Eukaryota |
GeneTreei | ENSGT00390000001979 |
HOGENOMi | CLU_070631_1_1_1 |
InParanoidi | Q63042 |
OMAi | KPCRSCT |
OrthoDBi | 1537996at2759 |
PhylomeDBi | Q63042 |
TreeFami | TF105271 |
Family and domain databases
Gene3Di | 1.20.120.310, 1 hit |
InterProi | View protein in InterPro IPR039799, ALR/ERV IPR036774, ERV/ALR_sulphydryl_oxid_sf IPR017905, ERV/ALR_sulphydryl_oxidase |
PANTHERi | PTHR12645, PTHR12645, 1 hit |
Pfami | View protein in Pfam PF04777, Evr1_Alr, 1 hit |
SUPFAMi | SSF69000, SSF69000, 1 hit |
PROSITEi | View protein in PROSITE PS51324, ERV_ALR, 1 hit |
i Sequence
Sequence statusi: Complete.
Q63042-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAPSEPAGF PRGSRFSFLP GGAHSEMTDD LVTDARGRGA RHRKDNAPAA
60 70 80 90 100
APAPKGLEHG KRPCRACVDF KSWMRTQQKR DIKFREDCPQ DREELGRNTW
110 120 130 140 150
AFLHTLAAYY PDMPTPEQQQ DMAQFIHIFS KFYPCEECAE DIRKRIDRSQ
160 170 180 190
PDTSTRVSFS QWLCRLHNEV NRKLGKPDFD CSRVDERWRD GWKDGSCD
Sequence cautioni
The sequence AAF12808 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA06399 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D30735 mRNA Translation: BAA06399.1 Different initiation. AF197192 Genomic DNA Translation: AAF12808.1 Different initiation. |
PIRi | I80184 |
RefSeqi | NP_037354.2, NM_013222.2 |
Genome annotation databases
Ensembli | ENSRNOT00000017917; ENSRNOP00000017917; ENSRNOG00000013370 |
GeneIDi | 27100 |
KEGGi | rno:27100 |
UCSCi | RGD:61845, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D30735 mRNA Translation: BAA06399.1 Different initiation. AF197192 Genomic DNA Translation: AAF12808.1 Different initiation. |
PIRi | I80184 |
RefSeqi | NP_037354.2, NM_013222.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OQC | X-ray | 1.80 | A/B/C/D | 74-198 | [»] | |
3R7C | X-ray | 2.40 | A/B/C/D | 74-198 | [»] | |
SMRi | Q63042 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000017917 |
Proteomic databases
PaxDbi | Q63042 |
PRIDEi | Q63042 |
Genome annotation databases
Ensembli | ENSRNOT00000017917; ENSRNOP00000017917; ENSRNOG00000013370 |
GeneIDi | 27100 |
KEGGi | rno:27100 |
UCSCi | RGD:61845, rat |
Organism-specific databases
CTDi | 2671 |
RGDi | 61845, Gfer |
Phylogenomic databases
eggNOGi | KOG3355, Eukaryota |
GeneTreei | ENSGT00390000001979 |
HOGENOMi | CLU_070631_1_1_1 |
InParanoidi | Q63042 |
OMAi | KPCRSCT |
OrthoDBi | 1537996at2759 |
PhylomeDBi | Q63042 |
TreeFami | TF105271 |
Enzyme and pathway databases
BRENDAi | 1.8.3.2, 5301 |
Miscellaneous databases
EvolutionaryTracei | Q63042 |
PROi | PR:Q63042 |
Gene expression databases
Bgeei | ENSRNOG00000013370, Expressed in testis and 22 other tissues |
Genevisiblei | Q63042, RN |
Family and domain databases
Gene3Di | 1.20.120.310, 1 hit |
InterProi | View protein in InterPro IPR039799, ALR/ERV IPR036774, ERV/ALR_sulphydryl_oxid_sf IPR017905, ERV/ALR_sulphydryl_oxidase |
PANTHERi | PTHR12645, PTHR12645, 1 hit |
Pfami | View protein in Pfam PF04777, Evr1_Alr, 1 hit |
SUPFAMi | SSF69000, SSF69000, 1 hit |
PROSITEi | View protein in PROSITE PS51324, ERV_ALR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ALR_RAT | |
Accessioni | Q63042Primary (citable) accession number: Q63042 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | December 16, 2008 | |
Last modified: | December 2, 2020 | |
This is version 134 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references