UniProtKB - Q63028 (ADDA_RAT)
Protein
Alpha-adducin
Gene
Add1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
GO - Molecular functioni
- actin filament binding Source: RGD
- calmodulin binding Source: UniProtKB-KW
- protein heterodimerization activity Source: RGD
- protein homodimerization activity Source: RGD
- spectrin binding Source: RGD
- structural constituent of cytoskeleton Source: RGD
- structural molecule activity Source: GO_Central
- T cell receptor binding Source: RGD
- transcription factor binding Source: RGD
GO - Biological processi
- actin filament bundle assembly Source: RGD
- barbed-end actin filament capping Source: RGD
- cell morphogenesis Source: RGD
- cellular response to calcium ion Source: RGD
- cellular response to retinoic acid Source: RGD
- cell volume homeostasis Source: RGD
- cytoskeleton organization Source: RGD
- erythrocyte differentiation Source: RGD
- hemoglobin metabolic process Source: RGD
- homeostasis of number of cells within a tissue Source: RGD
- in utero embryonic development Source: RGD
- ion transport Source: RGD
- multicellular organism growth Source: RGD
- negative regulation of actin filament polymerization Source: RGD
- positive regulation of adherens junction organization Source: RGD
- positive regulation of angiogenesis Source: RGD
- positive regulation of endocytosis Source: RGD
- positive regulation of establishment of endothelial barrier Source: RGD
- positive regulation of protein binding Source: RGD
Keywordsi
Molecular function | Actin-binding, Calmodulin-binding |
Enzyme and pathway databases
Reactomei | R-RNO-264870, Caspase-mediated cleavage of cytoskeletal proteins R-RNO-5223345, Miscellaneous transport and binding events |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-adducinAlternative name(s): Erythrocyte adducin subunit alpha |
Gene namesi | Name:Add1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2041, Add1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Cytoskeleton
- cytoskeleton By similarity
Cytoskeleton
- cytoskeleton Source: RGD
- F-actin capping protein complex Source: RGD
Nucleus
- nucleus Source: RGD
Plasma Membrane
- plasma membrane raft Source: GO_Central
Other locations
- adherens junction Source: RGD
- cytoplasm Source: RGD
- dendrite Source: RGD
- dendritic spine Source: RGD
- membrane Source: RGD
- perinuclear region of cytoplasm Source: RGD
- postsynaptic density Source: RGD
- synapse Source: RGD
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218532 | 1 – 735 | Alpha-adducinAdd BLAST | 735 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 12 | PhosphoserineCombined sources | 1 | |
Modified residuei | 59 | Phosphoserine; by PKABy similarity | 1 | |
Modified residuei | 64 | PhosphoserineBy similarity | 1 | |
Modified residuei | 331 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 334 | PhosphoserineBy similarity | 1 | |
Modified residuei | 353 | PhosphoserineBy similarity | 1 | |
Modified residuei | 355 | PhosphoserineCombined sources | 1 | |
Modified residuei | 358 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 364 | PhosphoserineBy similarity | 1 | |
Modified residuei | 366 | PhosphoserineCombined sources | 1 | |
Modified residuei | 408 | Phosphoserine; by PKABy similarity | 1 | |
Modified residuei | 427 | PhosphoserineBy similarity | 1 | |
Modified residuei | 429 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 431 | PhosphoserineBy similarity | 1 | |
Modified residuei | 436 | Phosphoserine; by PKABy similarity | 1 | |
Modified residuei | 445 | Phosphothreonine; by ROCK2By similarity | 1 | |
Modified residuei | 464 | PhosphoserineBy similarity | 1 | |
Modified residuei | 465 | PhosphoserineCombined sources | 1 | |
Modified residuei | 480 | Phosphothreonine; by ROCK2By similarity | 1 | |
Modified residuei | 481 | Phosphoserine; by PKABy similarity | 1 | |
Modified residuei | 586 | PhosphoserineCombined sources | 1 | |
Modified residuei | 600 | PhosphoserineCombined sources | 1 | |
Modified residuei | 605 | PhosphoserineBy similarity | 1 | |
Modified residuei | 610 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 613 | PhosphoserineBy similarity | 1 | |
Modified residuei | 614 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 705 | PhosphoserineBy similarity | 1 | |
Modified residuei | 708 | PhosphoserineBy similarity | 1 | |
Modified residuei | 712 | PhosphoserineBy similarity | 1 | |
Modified residuei | 714 | Phosphoserine; by PKCBy similarity | 1 | |
Modified residuei | 724 | Phosphoserine; by PKA and PKCBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | Q63028 |
PaxDbi | Q63028 |
PRIDEi | Q63028 |
PTM databases
iPTMneti | Q63028 |
PhosphoSitePlusi | Q63028 |
Interactioni
Subunit structurei
Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit.
GO - Molecular functioni
- actin filament binding Source: RGD
- calmodulin binding Source: UniProtKB-KW
- protein heterodimerization activity Source: RGD
- protein homodimerization activity Source: RGD
- spectrin binding Source: RGD
- T cell receptor binding Source: RGD
- transcription factor binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 246360, 1 interactor |
STRINGi | 10116.ENSRNOP00000018072 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 715 – 732 | Interaction with calmodulinSequence analysisAdd BLAST | 18 |
Domaini
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3699, Eukaryota |
InParanoidi | Q63028 |
OrthoDBi | 400524at2759 |
PhylomeDBi | Q63028 |
TreeFami | TF313003 |
Family and domain databases
Gene3Di | 3.40.225.10, 1 hit |
InterProi | View protein in InterPro IPR027766, ADD1 IPR001303, Aldolase_II/adducin_N IPR036409, Aldolase_II/adducin_N_sf |
PANTHERi | PTHR10672:SF4, PTHR10672:SF4, 1 hit |
Pfami | View protein in Pfam PF00596, Aldolase_II, 1 hit |
SMARTi | View protein in SMART SM01007, Aldolase_II, 1 hit |
SUPFAMi | SSF53639, SSF53639, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketNote: Additional isoforms seem to exist.
This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q63028-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNGDTRAAVV TSPPPTTAPH KERYFDRVDE NNPEYLRERN MAPDLRQDFN
60 70 80 90 100
MMEQKKRVSM ILQSPAFCEE LESMIQEQFK KGKNPTGLLA LQQIADFMTA
110 120 130 140 150
SVPNVYPAAP QGGMAALNMS LGMVTPVNDL RGSDSIAYDK GEKLLRCKLA
160 170 180 190 200
AFYRLADLFG WSQLIYNHIT TRVNSEQEHF LIVPFGLLYS EVTASSLVKV
210 220 230 240 250
NLQGDIVDRG STNLGVNQAG FTLHSAIYAA RPDAKCIVHI HTPAGAAVSA
260 270 280 290 300
MKCGLLPISP EALSLGEVAY HDYHGILVDE EEKILIQKNL GPKSKVLILR
310 320 330 340 350
NHGLVSVGES VEEAFYYIHN LVVACEIQVR TLASAGGPDN LVLLDPGKYK
360 370 380 390 400
AKSRSPGTPA GEGSGSPPKW QIGEQEFEAL MRMLDNLGYR TGYPYRYPAL
410 420 430 440 450
RERSKKYSDV EVPASVTGHS FASDGDSGTC SPLRHSFQKQ QREKTRWLNS
460 470 480 490 500
GRGDDASEEG QNGSSPKSKT KWTKEDGHRT STSAVPNLFV PLNTNPKEVQ
510 520 530 540 550
EMRNKIREQN LQDIKTAGPQ SQVLCGVMMD RSLVQGELVT ASKAIIEKEY
560 570 580 590 600
QPHVIVSTTG PNPFNTLTDR ELEEYRREVE RKQKGSEENL DETREQKEKS
610 620 630 640 650
PPDQSAVPNT PPSTPVKLEG GLPQEPTSRD GSDATTSKPT PPDLSPDEPS
660 670 680 690 700
EALAFPTVAE EAHASPDTTQ PLAEADPEPA SASAPGAEEV ASPATEEGSP
710 720 730
MDPGSDGSPG KSPSKKKKKF RTPSFLKKSK KKSDS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JSM7 | A0A0G2JSM7_RAT | Adducin 1 (Alpha), isoform CRA_b | Add1 rCG_36195 | 735 | Annotation score: | ||
D3ZZ99 | D3ZZ99_RAT | Alpha-adducin | Add1 | 678 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 633 – 636 | DATT → GCHA in CAA88907 (PubMed:8543181).Curated | 4 | |
Sequence conflicti | 654 | A → G in CAA58690 (PubMed:8624703).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 316 | Y → F in strain: Milan hypersensitive. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000179 | 621 – 632 | GLPQE…SRDGS → GDGCAKEYLLP in isoform 2. CuratedAdd BLAST | 12 | |
Alternative sequenceiVSP_000180 | 633 – 735 | Missing in isoform 2. CuratedAdd BLAST | 103 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49081 mRNA Translation: CAA88906.1 Z49082 mRNA Translation: CAA88907.1 BC107657 mRNA Translation: AAI07658.1 X83715 mRNA Translation: CAA58690.1 |
PIRi | S54147 |
RefSeqi | NP_058686.2, NM_016990.2 [Q63028-1] |
Genome annotation databases
GeneIDi | 24170 |
KEGGi | rno:24170 |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49081 mRNA Translation: CAA88906.1 Z49082 mRNA Translation: CAA88907.1 BC107657 mRNA Translation: AAI07658.1 X83715 mRNA Translation: CAA58690.1 |
PIRi | S54147 |
RefSeqi | NP_058686.2, NM_016990.2 [Q63028-1] |
3D structure databases
SMRi | Q63028 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 246360, 1 interactor |
STRINGi | 10116.ENSRNOP00000018072 |
PTM databases
iPTMneti | Q63028 |
PhosphoSitePlusi | Q63028 |
Proteomic databases
jPOSTi | Q63028 |
PaxDbi | Q63028 |
PRIDEi | Q63028 |
Genome annotation databases
GeneIDi | 24170 |
KEGGi | rno:24170 |
Organism-specific databases
CTDi | 118 |
RGDi | 2041, Add1 |
Phylogenomic databases
eggNOGi | KOG3699, Eukaryota |
InParanoidi | Q63028 |
OrthoDBi | 400524at2759 |
PhylomeDBi | Q63028 |
TreeFami | TF313003 |
Enzyme and pathway databases
Reactomei | R-RNO-264870, Caspase-mediated cleavage of cytoskeletal proteins R-RNO-5223345, Miscellaneous transport and binding events |
Miscellaneous databases
PROi | PR:Q63028 |
Family and domain databases
Gene3Di | 3.40.225.10, 1 hit |
InterProi | View protein in InterPro IPR027766, ADD1 IPR001303, Aldolase_II/adducin_N IPR036409, Aldolase_II/adducin_N_sf |
PANTHERi | PTHR10672:SF4, PTHR10672:SF4, 1 hit |
Pfami | View protein in Pfam PF00596, Aldolase_II, 1 hit |
SMARTi | View protein in SMART SM01007, Aldolase_II, 1 hit |
SUPFAMi | SSF53639, SSF53639, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ADDA_RAT | |
Accessioni | Q63028Primary (citable) accession number: Q63028 Secondary accession number(s): Q3B7D4, Q64722 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | December 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families