UniProtKB - Q63016 (LAT1_RAT)
Protein
Large neutral amino acids transporter small subunit 1
Gene
Slc7a5
Organism
Rattus norvegicus (Rat)
Status
Functioni
The heterodimer with SLC3A2 functions as sodium-independent, high-affinity transporter that mediates uptake of large neutral amino acids such as phenylalanine, tyrosine, L-DOPA, leucine, histidine, methionine and tryptophan (PubMed:9726963). Functions as an amino acid exchanger (By similarity). May play a role in the transport of L-DOPA across the blood-brain barrier (By similarity). May act as the major transporter of tyrosine in fibroblasts (By similarity). May mediate blood-to-retina L-leucine transport across the inner blood-retinal barrier (Probable). Can mediate the transport of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane. When associated with LAPTM4B, the heterodimer formed by SLC3A2 and SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation. Involved in the uptake of toxic methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the membrane (By similarity).By similarity1 Publication1 Publication
Miscellaneous
L-leucine uptake by TR-iBRB2 cells was inhibited by L-leucine, L-phenylalanine, L-methionine, L-isoleucine, L-valine, L-tyrosine, L-tryptophan, D-leucine, D-phenylalanine, D-methionine and by 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH) (a specific inhibitor of system L transport).
Kineticsi
- KM=14.1 µM for L-leucine1 Publication
GO - Molecular functioni
- amino acid transmembrane transporter activity Source: UniProtKB
- L-amino acid transmembrane transporter activity Source: GO_Central
- L-leucine transmembrane transporter activity Source: UniProtKB
- L-tryptophan transmembrane transporter activity Source: UniProtKB
- peptide antigen binding Source: UniProtKB
GO - Biological processi
- L-tryptophan transmembrane transport Source: UniProtKB
- neutral amino acid transport Source: UniProtKB
Keywordsi
Biological process | Amino-acid transport, Transport |
Enzyme and pathway databases
Reactomei | R-RNO-210991 Basigin interactions R-RNO-352230 Amino acid transport across the plasma membrane R-RNO-71240 Tryptophan catabolism |
SABIO-RKi | Q63016 |
Protein family/group databases
TCDBi | 2.A.3.8.1 the amino acid-polyamine-organocation (apc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Large neutral amino acids transporter small subunit 1Alternative name(s): 4F2 light chain Short name: 4F2 LC Short name: 4F2LC Integral membrane protein E16 Short name: Protein TA11 Publication L-type amino acid transporter 11 Publication Short name: LAT-11 Publication Solute carrier family 7 member 5 |
Gene namesi | Name:Slc7a5 Synonyms:Lat11 Publication, Mpe16, Ta11 Publication |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620639 Slc7a5 |
Subcellular locationi
Plasma membrane
- Apical cell membrane By similarity; Multi-pass membrane protein By similarity
- Cell membrane 2 Publications; Multi-pass membrane protein By similarity
Lysosome
- Lysosome membrane By similarity; Multi-pass membrane protein By similarity
Note: Located to the plasma membrane by SLC3A2/4F2hc. Localized to the apical membrane of placental syncytiotrophoblastic cells. Recruited to lysosomes by LAPTM4B (By similarity). Expressed in both luminal and abluminal membranes of brain capillary endothelial cells (By similarity).By similarity
Cytosol
- cytosol Source: Ensembl
Lysosome
- lysosomal membrane Source: UniProtKB-SubCell
Plasma Membrane
- amino acid transport complex Source: UniProtKB
- basal plasma membrane Source: ARUK-UCL
- basolateral plasma membrane Source: ARUK-UCL
- external side of apical plasma membrane Source: ARUK-UCL
- plasma membrane Source: UniProtKB
Other locations
- cell surface Source: ARUK-UCL
- integral component of membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 50 | CytoplasmicCuratedAdd BLAST | 50 | |
Transmembranei | 51 – 71 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 72 – 84 | ExtracellularCuratedAdd BLAST | 13 | |
Transmembranei | 85 – 105 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 106 – 127 | CytoplasmicCuratedAdd BLAST | 22 | |
Transmembranei | 128 – 148 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 149 – 170 | ExtracellularCuratedAdd BLAST | 22 | |
Transmembranei | 171 – 191 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 192 – 193 | CytoplasmicCurated | 2 | |
Transmembranei | 194 – 215 | HelicalBy similarityAdd BLAST | 22 | |
Topological domaini | 216 – 247 | ExtracellularCuratedAdd BLAST | 32 | |
Transmembranei | 248 – 268 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 269 – 281 | CytoplasmicCuratedAdd BLAST | 13 | |
Transmembranei | 282 – 302 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 303 – 329 | ExtracellularCuratedAdd BLAST | 27 | |
Transmembranei | 330 – 350 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 351 – 374 | CytoplasmicCuratedAdd BLAST | 24 | |
Transmembranei | 375 – 395 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 396 – 400 | ExtracellularCurated | 5 | |
Transmembranei | 401 – 421 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 422 – 435 | CytoplasmicCuratedAdd BLAST | 14 | |
Transmembranei | 436 – 456 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 457 – 462 | ExtracellularCurated | 6 | |
Transmembranei | 463 – 483 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 484 – 512 | CytoplasmicCuratedAdd BLAST | 29 |
Keywords - Cellular componenti
Cell membrane, Lysosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054272 | 1 – 512 | Large neutral amino acids transporter small subunit 1Add BLAST | 512 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 46 | PhosphothreonineBy similarity | 1 | |
Disulfide bondi | 165 | Interchain (with C-210 in SLC3A2)By similarity |
Keywords - PTMi
Disulfide bond, PhosphoproteinProteomic databases
jPOSTi | Q63016 |
PaxDbi | Q63016 |
PRIDEi | Q63016 |
Expressioni
Tissue specificityi
Expressed in hepatoma but not in normal liver. Also expressed in placenta, testis, brain, ovary, spleen, mammary gland, and uterus. In brain expressed on capillary endothelia in cerebral cortex. Expressed in jejunum mucosa and the epithelial cells of the jejunum, ileum and colon. Also expressed in the intestinal epithelial cell line IEC-6. Expressed in the brain, retina, inner blood-retinal barrier of retina, retinal vascular endothelial cells, and in the retinal capillary endothelial cell line TR-iBRB2.6 Publications
Inductioni
Expression induced in normal hepatic cells cultured in arginine-depleted medium.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000018824 Expressed in 10 organ(s), highest expression level in testis |
Genevisiblei | Q63016 RN |
Interactioni
Subunit structurei
Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc (PubMed:9726963).
Interacts with LAPTM4B; this recruits the heterodimer formed by SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation (By similarity).
By similarity1 PublicationProtein-protein interaction databases
BioGridi | 248432, 1 interactor |
IntActi | Q63016, 1 interactor |
STRINGi | 10116.ENSRNOP00000025784 |
Chemistry databases
BindingDBi | Q63016 |
Family & Domainsi
Sequence similaritiesi
Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1287 Eukaryota COG0531 LUCA |
GeneTreei | ENSGT00940000155581 |
HOGENOMi | HOG000098892 |
InParanoidi | Q63016 |
KOi | K13780 |
OMAi | HKAFGIH |
OrthoDBi | 621852at2759 |
PhylomeDBi | Q63016 |
TreeFami | TF313355 |
Family and domain databases
InterProi | View protein in InterPro IPR002293 AA/rel_permease1 IPR004760 L_AA_transporter |
Pfami | View protein in Pfam PF13520 AA_permease_2, 1 hit |
PIRSFi | PIRSF006060 AA_transporter, 1 hit |
TIGRFAMsi | TIGR00911 2A0308, 1 hit |
i Sequence
Sequence statusi: Complete.
Q63016-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAVAGAKRRA VAAPATTAAE EERQAREKML EARRGDGADP EGEGVTLQRN
60 70 80 90 100
ITLINGVAII VGTIIGSGIF VTPTGVLKEA GSPGLSLVVW AVCGVFSIVG
110 120 130 140 150
ALCYAELGTT ISKSGGDYAY MLEVYGSLPA FLKLWIELLI IRPSSQYIVA
160 170 180 190 200
LVFATYLLKP VFPTCPVPEE AAKLVACLCV LLLTAVNCYS VKAATRVQDA
210 220 230 240 250
FAAAKLLALA LIILLGFIQM GKDIGQGDAS NLHQKLSFEG TNLDVGNIVL
260 270 280 290 300
ALYSGLFAYG GWNYLNFVTE EMINPYRNLP LAIIISLPIV TLVYVLTNLA
310 320 330 340 350
YFTTLSTNQM LTSEAVAVDF GNYHLGVMSW IIPVFVGLSC FGSVNGSLFT
360 370 380 390 400
SSRLFFVGSR EGHLPSILSM IHPQLLTPVP SLVFTCVMTL MYAFSRDIFS
410 420 430 440 450
IINFFSFFNW LCVALAIIGM MWLRFKKPEL ERPIKVNLAL PVFFILACLF
460 470 480 490 500
LIAVSFWKTP LECGIGFAII LSGLPVYFFG VWWKNKPKWI LQVIFSVTVL
510
CQKLMQVVPQ ET
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB015432 mRNA Translation: BAA33035.1 U00995 mRNA Translation: AAA74411.1 |
RefSeqi | NP_059049.1, NM_017353.1 |
Genome annotation databases
Ensembli | ENSRNOT00000025784; ENSRNOP00000025784; ENSRNOG00000018824 |
GeneIDi | 50719 |
KEGGi | rno:50719 |
UCSCi | RGD:620639 rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB015432 mRNA Translation: BAA33035.1 U00995 mRNA Translation: AAA74411.1 |
RefSeqi | NP_059049.1, NM_017353.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGridi | 248432, 1 interactor |
IntActi | Q63016, 1 interactor |
STRINGi | 10116.ENSRNOP00000025784 |
Chemistry databases
BindingDBi | Q63016 |
ChEMBLi | CHEMBL4149 |
DrugCentrali | Q63016 |
Protein family/group databases
TCDBi | 2.A.3.8.1 the amino acid-polyamine-organocation (apc) family |
Proteomic databases
jPOSTi | Q63016 |
PaxDbi | Q63016 |
PRIDEi | Q63016 |
Genome annotation databases
Ensembli | ENSRNOT00000025784; ENSRNOP00000025784; ENSRNOG00000018824 |
GeneIDi | 50719 |
KEGGi | rno:50719 |
UCSCi | RGD:620639 rat |
Organism-specific databases
CTDi | 8140 |
RGDi | 620639 Slc7a5 |
Phylogenomic databases
eggNOGi | KOG1287 Eukaryota COG0531 LUCA |
GeneTreei | ENSGT00940000155581 |
HOGENOMi | HOG000098892 |
InParanoidi | Q63016 |
KOi | K13780 |
OMAi | HKAFGIH |
OrthoDBi | 621852at2759 |
PhylomeDBi | Q63016 |
TreeFami | TF313355 |
Enzyme and pathway databases
Reactomei | R-RNO-210991 Basigin interactions R-RNO-352230 Amino acid transport across the plasma membrane R-RNO-71240 Tryptophan catabolism |
SABIO-RKi | Q63016 |
Miscellaneous databases
PROi | PR:Q63016 |
Gene expression databases
Bgeei | ENSRNOG00000018824 Expressed in 10 organ(s), highest expression level in testis |
Genevisiblei | Q63016 RN |
Family and domain databases
InterProi | View protein in InterPro IPR002293 AA/rel_permease1 IPR004760 L_AA_transporter |
Pfami | View protein in Pfam PF13520 AA_permease_2, 1 hit |
PIRSFi | PIRSF006060 AA_transporter, 1 hit |
TIGRFAMsi | TIGR00911 2A0308, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LAT1_RAT | |
Accessioni | Q63016Primary (citable) accession number: Q63016 Secondary accession number(s): Q9QWL4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | January 24, 2001 | |
Last modified: | October 16, 2019 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families