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Protein

Estrogen receptor beta

Gene

Esr2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform 3 and isoform 4 are unable to bind DNA and activate transcription due to the truncation of the DNA binding domain. Isoform 2 shows loss of ligand binding affinity and suppresses ER-alpha and ER-beta1 mediated transcriptional activation and may act as a dominant negative regulator of estrogen action.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi149 – 214Nuclear receptorPROSITE-ProRule annotationAdd BLAST66
Zinc fingeri149 – 169NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri185 – 209NR C4-typePROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Receptor
Biological processTranscription, Transcription regulation
LigandLipid-binding, Metal-binding, Steroid-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Estrogen receptor beta
Short name:
ER-beta
Alternative name(s):
Nuclear receptor subfamily 3 group A member 2
Gene namesi
Name:Esr2
Synonyms:Erbeta, Nr3a2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2582 Esr2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3021
GuidetoPHARMACOLOGYi621

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000536461 – 530Estrogen receptor betaAdd BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61Phosphoserine; alternateBy similarity1
Glycosylationi61O-linked (GlcNAc) serine; alternateBy similarity1
Modified residuei87Phosphoserine; by MAPKBy similarity1
Modified residuei105Phosphoserine; by MAPKBy similarity1

Post-translational modificationi

Phosphorylation at Ser-87 and Ser-105 recruits NCOA1.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ62986
PRIDEiQ62986

PTM databases

PhosphoSitePlusiQ62986

Expressioni

Tissue specificityi

Expressed in prostate, ovary, lung, liver, kidney, fat, bone, brain, uterus and testis.

Interactioni

Subunit structurei

Binds DNA as a homodimer. Can form a heterodimer with ESR1. Interacts with NCOA1, NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with PELP1, UBE1C and AKAP13. Interacts with DNTTIP2 (By similarity). Interacts with CCDC62 in the presence of estradiol/E2; this interaction seems to enhance the transcription of target genes. Interacts with PRMT2. Interacts with CCAR2 (via N-terminus) in a ligand-independent manner (By similarity). Interacts with DNAAF4. Interacts with RBM39, in the presence of estradiol (E2) (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
UbbP629892EBI-15938489,EBI-7038538

GO - Molecular functioni

Protein-protein interaction databases

BioGridi247214, 6 interactors
DIPiDIP-60383N
IntActiQ62986, 2 interactors
STRINGi10116.ENSRNOP00000043144

Chemistry databases

BindingDBiQ62986

Structurei

Secondary structure

1530
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ62986
SMRiQ62986
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62986

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini264 – 498NR LBDPROSITE-ProRule annotationAdd BLAST235

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 148ModulatingAdd BLAST148

Domaini

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri149 – 169NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri185 – 209NR C4-typePROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3575 Eukaryota
ENOG410XRZC LUCA
HOVERGENiHBG108344
InParanoidiQ62986
KOiK08551

Family and domain databases

Gene3Di3.30.50.10, 1 hit
InterProiView protein in InterPro
IPR028355 ER-beta/gamma
IPR021064 Estrogen_rcpt_beta_N
IPR035500 NHR_like_dom_sf
IPR000536 Nucl_hrmn_rcpt_lig-bd
IPR001723 Nuclear_hrmn_rcpt
IPR024178 Oest_rcpt/oest-rel_rcp
IPR001628 Znf_hrmn_rcpt
IPR013088 Znf_NHR/GATA
PfamiView protein in Pfam
PF12497 ERbeta_N, 1 hit
PF00104 Hormone_recep, 1 hit
PF00105 zf-C4, 1 hit
PIRSFiPIRSF500102 ER-b, 1 hit
PIRSF002527 ER-like_NR, 1 hit
PRINTSiPR00398 STRDHORMONER
PR00047 STROIDFINGER
SMARTiView protein in SMART
SM00430 HOLI, 1 hit
SM00399 ZnF_C4, 1 hit
SUPFAMiSSF48508 SSF48508, 1 hit
PROSITEiView protein in PROSITE
PS51843 NR_LBD, 1 hit
PS00031 NUCLEAR_REC_DBD_1, 1 hit
PS51030 NUCLEAR_REC_DBD_2, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Additional isoforms seem to exist.

This entry has 5 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q62986-1) [UniParc]FASTAAdd to basket
Also known as: Beta1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEIKNSPSSL SSPASYNCSQ SILPLEHGPI YIPSSYVDNR HEYSAMTFYS
60 70 80 90 100
PAVMNYSVPG STSNLDGGPV RLSTSPNVLW PTSGHLSPLA THCQSSLLYA
110 120 130 140 150
EPQKSPWCEA RSLEHTLPVN RETLKRKLSG SSCASPVTSP NAKRDAHFCP
160 170 180 190 200
VCSDYASGYH YGVWSCEGCK AFFKRSIQGH NDYICPATNQ CTIDKNRRKS
210 220 230 240 250
CQACRLRKCY EVGMVKCGSR RERCGYRIVR RQRSSSEQVH CLSKAKRNGG
260 270 280 290 300
HAPRVKELLL STLSPEQLVL TLLEAEPPNV LVSRPSMPFT EASMMMSLTK
310 320 330 340 350
LADKELVHMI GWAKKIPGFV ELSLLDQVRL LESCWMEVLM VGLMWRSIDH
360 370 380 390 400
PGKLIFAPDL VLDRDEGKCV EGILEIFDML LATTSRFREL KLQHKEYLCV
410 420 430 440 450
KAMILLNSSM YPLASANQEA ESSRKLTHLL NAVTDALVWV IAKSGISSQQ
460 470 480 490 500
QSVRLANLLM LLSHVRHISN KGMEHLLSMK CKNVVPVYDL LLEMLNAHTL
510 520 530
RGYKSSISGS ECSSTEDSKN KESSQNLQSQ
Length:530
Mass (Da):59,152
Last modified:July 15, 1999 - v2
Checksum:i36F269D9FD773DA9
GO
Isoform 2 (identifier: Q62986-2) [UniParc]FASTAAdd to basket
Also known as: Beta2

The sequence of this isoform differs from the canonical sequence as follows:
     364-364: R → RSSEDPHWHVAQMKSAAPR

Show »
Length:548
Mass (Da):61,168
Checksum:iE9E636A7E317CB3B
GO
Isoform 3 (identifier: Q62986-3) [UniParc]FASTAAdd to basket
Also known as: Beta1-delta3

The sequence of this isoform differs from the canonical sequence as follows:
     179-217: Missing.

Show »
Length:491
Mass (Da):54,663
Checksum:i6FA4105E37B02A03
GO
Isoform 4 (identifier: Q62986-4) [UniParc]FASTAAdd to basket
Also known as: Beta2-delta3

The sequence of this isoform differs from the canonical sequence as follows:
     179-217: Missing.
     364-364: R → RSSEDPHWHVAQMKSAAPR

Show »
Length:509
Mass (Da):56,679
Checksum:iE5DA93F80B6E6BAC
GO
Isoform 5 (identifier: Q62986-5) [UniParc]FASTAAdd to basket
Also known as: Beta1-delta4

The sequence of this isoform differs from the canonical sequence as follows:
     219-318: Missing.

Show »
Length:430
Mass (Da):47,904
Checksum:i3136E9B67BA0E01F
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q59IV8Q59IV8_RAT
Estrogen receptor 2 beta, isoform C...
Esr2 ERb2, rCG_61423
567Annotation score:
Q59IV9Q59IV9_RAT
Estrogen receptor 2 beta, isoform C...
Esr2 ERb1, rCG_61423
549Annotation score:
D4AEI3D4AEI3_RAT
Estrogen receptor beta
Esr2
446Annotation score:
D3ZQD5D3ZQD5_RAT
Estrogen receptor beta
Esr2
464Annotation score:

Sequence cautioni

The sequence CAA05631 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72L → Q (Ref. 3) Curated1
Sequence conflicti72L → Q (PubMed:9492041).Curated1
Sequence conflicti150P → A (Ref. 3) Curated1
Sequence conflicti150P → A (PubMed:9492041).Curated1
Sequence conflicti165S → P in CAA05631 (Ref. 3) Curated1
Sequence conflicti505S → P in CAA05631 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003697179 – 217Missing in isoform 3 and isoform 4. CuratedAdd BLAST39
Alternative sequenceiVSP_003698219 – 318Missing in isoform 5. CuratedAdd BLAST100
Alternative sequenceiVSP_003699364R → RSSEDPHWHVAQMKSAAPR in isoform 2 and isoform 4. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57439 mRNA Translation: AAC52602.1
AB012721 mRNA Translation: BAA25431.1
AJ002602 Genomic DNA Translation: CAA05631.1 Different initiation.
AJ002603 Genomic DNA Translation: CAA05632.1
AF042058 mRNA Translation: AAB97424.1
AF042059 mRNA Translation: AAB97425.1
AF042060 mRNA Translation: AAB97426.1
AF042061 mRNA Translation: AAB97427.1
AF161187 mRNA Translation: AAD47637.1
PIRiJW0046
RefSeqiNP_036886.1, NM_012754.1
UniGeneiRn.37460

Genome annotation databases

GeneIDi25149
KEGGirno:25149
UCSCiRGD:2582 rat [Q62986-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57439 mRNA Translation: AAC52602.1
AB012721 mRNA Translation: BAA25431.1
AJ002602 Genomic DNA Translation: CAA05631.1 Different initiation.
AJ002603 Genomic DNA Translation: CAA05632.1
AF042058 mRNA Translation: AAB97424.1
AF042059 mRNA Translation: AAB97425.1
AF042060 mRNA Translation: AAB97426.1
AF042061 mRNA Translation: AAB97427.1
AF161187 mRNA Translation: AAD47637.1
PIRiJW0046
RefSeqiNP_036886.1, NM_012754.1
UniGeneiRn.37460

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HJ1X-ray2.30A255-509[»]
1QKNX-ray2.25A255-509[»]
2J7XX-ray2.10A255-509[»]
2J7YX-ray1.80A255-509[»]
ProteinModelPortaliQ62986
SMRiQ62986
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247214, 6 interactors
DIPiDIP-60383N
IntActiQ62986, 2 interactors
STRINGi10116.ENSRNOP00000043144

Chemistry databases

BindingDBiQ62986
ChEMBLiCHEMBL3021
GuidetoPHARMACOLOGYi621

PTM databases

PhosphoSitePlusiQ62986

Proteomic databases

PaxDbiQ62986
PRIDEiQ62986

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25149
KEGGirno:25149
UCSCiRGD:2582 rat [Q62986-1]

Organism-specific databases

CTDi2100
RGDi2582 Esr2

Phylogenomic databases

eggNOGiKOG3575 Eukaryota
ENOG410XRZC LUCA
HOVERGENiHBG108344
InParanoidiQ62986
KOiK08551

Miscellaneous databases

EvolutionaryTraceiQ62986
PROiPR:Q62986

Family and domain databases

Gene3Di3.30.50.10, 1 hit
InterProiView protein in InterPro
IPR028355 ER-beta/gamma
IPR021064 Estrogen_rcpt_beta_N
IPR035500 NHR_like_dom_sf
IPR000536 Nucl_hrmn_rcpt_lig-bd
IPR001723 Nuclear_hrmn_rcpt
IPR024178 Oest_rcpt/oest-rel_rcp
IPR001628 Znf_hrmn_rcpt
IPR013088 Znf_NHR/GATA
PfamiView protein in Pfam
PF12497 ERbeta_N, 1 hit
PF00104 Hormone_recep, 1 hit
PF00105 zf-C4, 1 hit
PIRSFiPIRSF500102 ER-b, 1 hit
PIRSF002527 ER-like_NR, 1 hit
PRINTSiPR00398 STRDHORMONER
PR00047 STROIDFINGER
SMARTiView protein in SMART
SM00430 HOLI, 1 hit
SM00399 ZnF_C4, 1 hit
SUPFAMiSSF48508 SSF48508, 1 hit
PROSITEiView protein in PROSITE
PS51843 NR_LBD, 1 hit
PS00031 NUCLEAR_REC_DBD_1, 1 hit
PS51030 NUCLEAR_REC_DBD_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiESR2_RAT
AccessioniPrimary (citable) accession number: Q62986
Secondary accession number(s): O35784
, O35785, O55015, O55016, O70195, Q9R185
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 15, 1999
Last modified: November 7, 2018
This is version 177 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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