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UniProtKB - Q62969 (PTGIS_RAT)
Protein
Prostacyclin synthase
Gene
Ptgis
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes the biosynthesis and metabolism of eicosanoids. Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2), a potent mediator of vasodilation and inhibitor of platelet aggregation. Additionally, displays dehydratase activity, toward hydroperoxyeicosatetraenoates (HPETEs), especially toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE).
By similarityCatalytic activityi
- EC:5.3.99.4By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:4.2.1.152By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
hemeBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 107 | SubstrateBy similarity | 1 | |
Binding sitei | 113 | Substrate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 288 | SubstrateBy similarity | 1 | |
Binding sitei | 383 | SubstrateBy similarity | 1 | |
Metal bindingi | 442 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- heme binding Source: UniProtKB
- hydroperoxy icosatetraenoate dehydratase activity Source: UniProtKB-EC
- iron ion binding Source: InterPro
- monooxygenase activity Source: InterPro
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Source: InterPro
- prostaglandin-I synthase activity Source: RGD
GO - Biological processi
- apoptotic signaling pathway Source: RGD
- cellular response to hypoxia Source: UniProtKB
- cellular response to interleukin-1 Source: UniProtKB
- cellular response to interleukin-6 Source: UniProtKB
- decidualization Source: RGD
- embryo implantation Source: RGD
- icosanoid metabolic process Source: RGD
- negative regulation of inflammatory response Source: UniProtKB
- negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
- negative regulation of nitric oxide biosynthetic process Source: UniProtKB
- positive regulation of angiogenesis Source: UniProtKB
- positive regulation of execution phase of apoptosis Source: UniProtKB
- positive regulation of peroxisome proliferator activated receptor signaling pathway Source: UniProtKB
- prostaglandin biosynthetic process Source: RGD
- response to hypoxia Source: RGD
Keywordsi
Molecular function | Isomerase, Lyase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
BRENDAi | 5.3.99.4, 5301 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Ptgis Synonyms:Cyp8 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3438, Ptgis |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- endoplasmic reticulum membrane Source: UniProtKB
Extracellular region or secreted
- extracellular space Source: RGD
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- caveola Source: UniProtKB
Other locations
- cytoplasm Source: RGD
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 1 – 21 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051912 | 1 – 501 | Prostacyclin synthaseAdd BLAST | 501 |
Proteomic databases
PaxDbi | Q62969 |
PRIDEi | Q62969 |
PTM databases
PhosphoSitePlusi | Q62969 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 247559, 1 interactor |
IntActi | Q62969, 1 interactor |
STRINGi | 10116.ENSRNOP00000010891 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 359 – 360 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0684, Eukaryota |
InParanoidi | Q62969 |
OrthoDBi | 572303at2759 |
PhylomeDBi | Q62969 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR024204, Cyt_P450_CYP7A1-type IPR002403, Cyt_P450_E_grp-IV IPR036396, Cyt_P450_sf IPR027286, PTGIS |
PANTHERi | PTHR24306:SF4, PTHR24306:SF4, 1 hit |
Pfami | View protein in Pfam PF00067, p450, 2 hits |
PIRSFi | PIRSF000047, Cytochrome_CYPVIIA1, 1 hit PIRSF500628, PTGIS, 1 hit |
PRINTSi | PR00465, EP450IV PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
i Sequence
Sequence statusi: Complete.
Q62969-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSWAALLGLL AVLLLLLLLL SRRRARRPGE PPLDLGSIPW LGHALEFGKD
60 70 80 90 100
AASFLTRMKE KHGDIFTVLV GGRYVTVLLD PHSYDTVVWD LRTRLDFHPY
110 120 130 140 150
AIFLMERIFD LQLPNFNPSE EKARMKPTLM HKDLQALTEA MYTNLRTVLL
160 170 180 190 200
GDSTEGGSGW QEKGLLEFSY SSLLSAGYLT LYGVEASPRT HESQALDRDH
210 220 230 240 250
SADVFRTFRQ LDLMLPKLAR GSLSVGDKDH ACSVKSRLWK LLSPAGLASR
260 270 280 290 300
ADRSSWLESY LRHLEEMGVS EDMQARALVL QLWATQGNMG PTAFWLLLFL
310 320 330 340 350
LKNPEALDAV HAELKRIVWQ AEKPVLQMTA LPQKILDSMP VLDSVLNETL
360 370 380 390 400
RLTAAPFITR EVMADLALPM ADRREFSLRR GDRLLLFPFL SPQKDPEIYT
410 420 430 440 450
EPEVFKYNRF LNPDGSEKKD FYKDGKRLKN YNMPWGAGHN QCLGKSYAIN
460 470 480 490 500
SIKQFVVLLL THFDLELVSE DTEVPEFDLS RYGFGLMQPE EDVPIRYRTR
L
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U53855 mRNA Translation: AAB02322.1 BC061814 mRNA Translation: AAH61814.1 |
RefSeqi | NP_113745.1, NM_031557.2 |
Genome annotation databases
GeneIDi | 25527 |
KEGGi | rno:25527 |
UCSCi | RGD:3438, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U53855 mRNA Translation: AAB02322.1 BC061814 mRNA Translation: AAH61814.1 |
RefSeqi | NP_113745.1, NM_031557.2 |
3D structure databases
SMRi | Q62969 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 247559, 1 interactor |
IntActi | Q62969, 1 interactor |
STRINGi | 10116.ENSRNOP00000010891 |
PTM databases
PhosphoSitePlusi | Q62969 |
Proteomic databases
PaxDbi | Q62969 |
PRIDEi | Q62969 |
Genome annotation databases
GeneIDi | 25527 |
KEGGi | rno:25527 |
UCSCi | RGD:3438, rat |
Organism-specific databases
CTDi | 5740 |
RGDi | 3438, Ptgis |
Phylogenomic databases
eggNOGi | KOG0684, Eukaryota |
InParanoidi | Q62969 |
OrthoDBi | 572303at2759 |
PhylomeDBi | Q62969 |
Enzyme and pathway databases
BRENDAi | 5.3.99.4, 5301 |
Miscellaneous databases
PROi | PR:Q62969 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR024204, Cyt_P450_CYP7A1-type IPR002403, Cyt_P450_E_grp-IV IPR036396, Cyt_P450_sf IPR027286, PTGIS |
PANTHERi | PTHR24306:SF4, PTHR24306:SF4, 1 hit |
Pfami | View protein in Pfam PF00067, p450, 2 hits |
PIRSFi | PIRSF000047, Cytochrome_CYPVIIA1, 1 hit PIRSF500628, PTGIS, 1 hit |
PRINTSi | PR00465, EP450IV PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PTGIS_RAT | |
Accessioni | Q62969Primary (citable) accession number: Q62969 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | November 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families