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Protein

Prostacyclin synthase

Gene

Ptgis

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).By similarity

Catalytic activityi

(5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-6,9-alpha-epoxy-11-alpha,15-dihydroxyprosta-5,13-dienoate.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107SubstrateBy similarity1
Binding sitei113Substrate; via amide nitrogenBy similarity1
Binding sitei288SubstrateBy similarity1
Binding sitei383SubstrateBy similarity1
Metal bindingi442Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi5.3.99.4 5301

Names & Taxonomyi

Protein namesi
Recommended name:
Prostacyclin synthase (EC:5.3.99.4By similarity)
Alternative name(s):
Prostaglandin I2 synthase
Gene namesi
Name:Ptgis
Synonyms:Cyp8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3438 Ptgis

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000519121 – 501Prostacyclin synthaseAdd BLAST501

Proteomic databases

PaxDbiQ62969
PRIDEiQ62969

PTM databases

PhosphoSitePlusiQ62969

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010891

Structurei

3D structure databases

ProteinModelPortaliQ62969
SMRiQ62969
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni359 – 360Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0684 Eukaryota
COG2124 LUCA
HOGENOMiHOG000231026
HOVERGENiHBG051100
InParanoidiQ62969
KOiK01831
PhylomeDBiQ62969

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR024204 Cyt_P450_CYP7A1-type
IPR002403 Cyt_P450_E_grp-IV
IPR036396 Cyt_P450_sf
IPR027286 PTGIS
PANTHERiPTHR24306:SF4 PTHR24306:SF4, 1 hit
PfamiView protein in Pfam
PF00067 p450, 2 hits
PIRSFiPIRSF000047 Cytochrome_CYPVIIA1, 1 hit
PIRSF500628 PTGIS, 1 hit
PRINTSiPR00465 EP450IV
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit

Sequencei

Sequence statusi: Complete.

Q62969-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWAALLGLL AVLLLLLLLL SRRRARRPGE PPLDLGSIPW LGHALEFGKD
60 70 80 90 100
AASFLTRMKE KHGDIFTVLV GGRYVTVLLD PHSYDTVVWD LRTRLDFHPY
110 120 130 140 150
AIFLMERIFD LQLPNFNPSE EKARMKPTLM HKDLQALTEA MYTNLRTVLL
160 170 180 190 200
GDSTEGGSGW QEKGLLEFSY SSLLSAGYLT LYGVEASPRT HESQALDRDH
210 220 230 240 250
SADVFRTFRQ LDLMLPKLAR GSLSVGDKDH ACSVKSRLWK LLSPAGLASR
260 270 280 290 300
ADRSSWLESY LRHLEEMGVS EDMQARALVL QLWATQGNMG PTAFWLLLFL
310 320 330 340 350
LKNPEALDAV HAELKRIVWQ AEKPVLQMTA LPQKILDSMP VLDSVLNETL
360 370 380 390 400
RLTAAPFITR EVMADLALPM ADRREFSLRR GDRLLLFPFL SPQKDPEIYT
410 420 430 440 450
EPEVFKYNRF LNPDGSEKKD FYKDGKRLKN YNMPWGAGHN QCLGKSYAIN
460 470 480 490 500
SIKQFVVLLL THFDLELVSE DTEVPEFDLS RYGFGLMQPE EDVPIRYRTR

L
Length:501
Mass (Da):57,128
Last modified:November 1, 1996 - v1
Checksum:iD2D85ADF3C464863
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53855 mRNA Translation: AAB02322.1
BC061814 mRNA Translation: AAH61814.1
RefSeqiNP_113745.1, NM_031557.2
UniGeneiRn.73051

Genome annotation databases

GeneIDi25527
KEGGirno:25527
UCSCiRGD:3438 rat

Similar proteinsi

Entry informationi

Entry nameiPTGIS_RAT
AccessioniPrimary (citable) accession number: Q62969
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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