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Protein

RanBP-type and C3HC4-type zinc finger-containing protein 1

Gene

Rbck1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types (By similarity). E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome.By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri188 – 220RanBP2-typePROSITE-ProRule annotationAdd BLAST33
Zinc fingeri280 – 325RING-type 1PROSITE-ProRule annotationAdd BLAST46
Zinc fingeri360 – 409IBR-typeAdd BLAST50
Zinc fingeri435 – 461RING-type 2PROSITE-ProRule annotationAdd BLAST27

GO - Molecular functioni

  • double-stranded DNA binding Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • protein kinase C binding Source: RGD
  • ubiquitin binding Source: UniProtKB
  • ubiquitin-protein transferase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.3.2.B10 5301

Names & Taxonomyi

Protein namesi
Recommended name:
RanBP-type and C3HC4-type zinc finger-containing protein 1 (EC:2.3.2.27)
Alternative name(s):
Heme-oxidized IRP2 ubiquitin ligase 1 homolog
Short name:
HOIL-1
Protein kinase C-binding protein beta-15
RBCC protein interacting with PKC
RING-type E3 ubiquitin transferase HOIL-1Curated
Ubiquitin-conjugating enzyme 7-interacting protein 3
Gene namesi
Name:Rbck1
Synonyms:Pkcbpb15, Rbck, Ubce7ip3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi708404 Rbck1

Subcellular locationi

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi303C → G: Greatly reduces auto-ubiquitination. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000562971 – 508RanBP-type and C3HC4-type zinc finger-containing protein 1Add BLAST508

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei50PhosphoserineBy similarity1
Modified residuei328PhosphotyrosineBy similarity1

Post-translational modificationi

Auto-ubiquitinated. Auto-ubiquitination leads to degradation by the proteasome.1 Publication
Phosphorylated. In vitro, phosphorylation inhibits auto-ubiquitination activity.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ62921
PRIDEiQ62921

PTM databases

iPTMnetiQ62921
PhosphoSitePlusiQ62921

Expressioni

Tissue specificityi

Widely expressed.

Interactioni

Subunit structurei

Forms homodimers in vitro (By similarity). Component of the LUBAC complex (linear ubiquitin chain assembly complex) which consists of SHARPIN, RBCK1 and RNF31. LUBAC has a MW of approximately 600 kDa suggesting a heteromultimeric assembly of its subunits. Interacts with beta-I-type (PRKCB1) and zeta-type protein kinase C (PRKCZ) and with UBE2L3. Interacts with IREB2 only in iron-rich conditions. Associates with the TNF-R1 signaling complex (TNF-RSC) in a stimulation-dependent manner. Interacts with EYA1, TAB2, TAB3, MAP3K7 TRAF6 and RIPK1. Interacts with IRF3 (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein kinase C binding Source: RGD
  • ubiquitin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi248810, 4 interactors
IntActiQ62921, 2 interactors
MINTiQ62921
STRINGi10116.ENSRNOP00000047637

Structurei

3D structure databases

ProteinModelPortaliQ62921
SMRiQ62921
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 119Ubiquitin-likePROSITE-ProRule annotationAdd BLAST65

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 268Interaction with TAB2By similarityAdd BLAST268
Regioni1 – 218Interaction with IRF3By similarityAdd BLAST218
Regioni69 – 131Interaction with RNF31By similarityAdd BLAST63

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili231 – 260Sequence analysisAdd BLAST30

Domaini

The RanBP2-type zinc finger, also called Npl4 zinc finger (NZF), mediates binding to 'Met-1'-linked polyubiquitins.By similarity
The UBL domain mediates association with RNF31 via interaction with its UBA domain.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri188 – 220RanBP2-typePROSITE-ProRule annotationAdd BLAST33
Zinc fingeri280 – 325RING-type 1PROSITE-ProRule annotationAdd BLAST46
Zinc fingeri360 – 409IBR-typeAdd BLAST50
Zinc fingeri435 – 461RING-type 2PROSITE-ProRule annotationAdd BLAST27

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1815 Eukaryota
ENOG410XP9Y LUCA
HOVERGENiHBG061515
InParanoidiQ62921
KOiK10630
PhylomeDBiQ62921

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR002867 IBR_dom
IPR026261 RBCK1
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
IPR027370 Znf-RING_LisH
IPR001876 Znf_RanBP2
IPR036443 Znf_RanBP2_sf
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR22770:SF35 PTHR22770:SF35, 1 hit
PfamiView protein in Pfam
PF01485 IBR, 1 hit
PF13445 zf-RING_UBOX, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SM00547 ZnF_RBZ, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
SSF90209 SSF90209, 1 hit
PROSITEiView protein in PROSITE
PS50053 UBIQUITIN_2, 1 hit
PS01358 ZF_RANBP2_1, 1 hit
PS50199 ZF_RANBP2_2, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q62921-1) [UniParc]FASTAAdd to basket
Also known as: RBCK1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEKTKKAEE MALSLARAVT GGDEQAAIKY ATWLAEQKVP LRVQVKPEVS
60 70 80 90 100
PTQDIRLCVS VEDAYMHTVT IWLTVRPDMT VASLKDMVFL DYGFPPSLQQ
110 120 130 140 150
WVVGQRLARD QETLHSHGIR RNGDSAYLYL LSARNTSLNP QELQRQRQLR
160 170 180 190 200
MLEDLGFKDL TLQPRGPLEP VLPKPRTHQE TGQPDAAPES PPVGWQCPGC
210 220 230 240 250
TFINKPTRPG CEMCCRARPE AYQIPASYQP DEEERARLAG EEEALRQYEQ
260 270 280 290 300
RKQQQQEGNY LQHVQLEQRS LVLNTEPAEC PVCYSVLAPG EAVVLRECLH
310 320 330 340 350
TFCRECLQGT IRNSQEAEVS CPFIDNTYSC PGKLLEREIR ALLSPEDYQR
360 370 380 390 400
FLDLGVSIAE NRSTLSYHCK TPDCRGWCFF EDDVNEFTCP VCTRVNCLLC
410 420 430 440 450
KAIHERMNCR EYQDDLAHRA RNDVAAQQTT EMLRVMLQQG EAMYCPQCRI
460 470 480 490 500
VVQKKDGCDW IRCTVCHTEI CWVTKGPRWG PGGPGDTSGG CRCRVNGIPC

HPSCQNCH
Length:508
Mass (Da):57,685
Last modified:January 15, 2008 - v3
Checksum:iD2E407B254E9FDA6
GO
Isoform 2 (identifier: Q62921-2) [UniParc]FASTAAdd to basket
Also known as: RBCK2

The sequence of this isoform differs from the canonical sequence as follows:
     251-270: RKQQQQEGNYLQHVQLEQRS → GVPAGYHPKQPGGGGILPLH
     271-508: Missing.

Show »
Length:270
Mass (Da):30,278
Checksum:iD689295BBC4ED744
GO

Sequence cautioni

The sequence AAC72243 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA33957 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005769251 – 270RKQQQ…LEQRS → GVPAGYHPKQPGGGGILPLH in isoform 2. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_005770271 – 508Missing in isoform 2. 1 PublicationAdd BLAST238

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48248 mRNA Translation: AAC72243.1 Different initiation.
AB007133 Genomic DNA Translation: BAA33953.1
AB011369 mRNA Translation: BAA33957.1 Different initiation.
PIRiJC5983
RefSeqiNP_068532.2, NM_021764.1 [Q62921-1]
UniGeneiRn.37161

Genome annotation databases

GeneIDi60383
KEGGirno:60383
UCSCiRGD:708404 rat [Q62921-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHOIL1_RAT
AccessioniPrimary (citable) accession number: Q62921
Secondary accession number(s): Q9QWN4, Q9Z334
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 15, 2008
Last modified: June 20, 2018
This is version 142 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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