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Protein

Peripheral plasma membrane protein CASK

Gene

Cask

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TBR1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins, and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Note: Unlike other protein kinases, does not require a divalent cation such as magnesium for catalytic activity.By similarity

Activity regulationi

Differs from archetypal CaMK members in that the kinase domain exhibits a constitutively active conformation and the autoinhibitory region does not engage in direct contact with the ATP-binding cleft, although it still binds Ca2+/CAM.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41ATPPROSITE-ProRule annotation1
Active sitei141By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 26ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cerebral cortex development Source: RGD
  • positive regulation of dendritic spine morphogenesis Source: RGD
  • positive regulation of insulin secretion Source: RGD
  • protein localization to plasma membrane Source: RGD
  • regulation of transcription by RNA polymerase II Source: RGD

Keywordsi

Molecular functionCalmodulin-binding, Kinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1 5301

Names & Taxonomyi

Protein namesi
Recommended name:
Peripheral plasma membrane protein CASK (EC:2.7.11.1)
Alternative name(s):
Calcium/calmodulin-dependent serine protein kinase
Gene namesi
Name:Cask
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi62004 Cask

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000945701 – 909Peripheral plasma membrane protein CASKAdd BLAST909

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei51PhosphoserineBy similarity1
Modified residuei151Phosphoserine; by autocatalysisBy similarity1
Modified residuei155Phosphoserine; by autocatalysisBy similarity1
Modified residuei182PhosphothreonineBy similarity1
Modified residuei313PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ62915
PRIDEiQ62915

PTM databases

iPTMnetiQ62915
PhosphoSitePlusiQ62915
SwissPalmiQ62915

Expressioni

Tissue specificityi

Expressed in the foot process layer of podocytes in the kidney glomerulus and in tubules (at protein level). Detected in brain and neurons.3 Publications

Interactioni

Subunit structurei

Binds WHRN and NRXN1 cytosolic tail. Interacts with CASKIN1, APBA1, LIN7(A/B/C), and L27 domain of DLG1 and isoform 2 of DLG4. CASK and LIN7 form two mutually exclusive tripartite complexes with APBA1 or CASKIN1. Interacts with FCHSD2. Identified in a complex with ACTN4, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1. Interacts with TSPYL2. Part of a complex containing CASK, TBR1 and TSPYL2 (By similarity). Interacts with KIRREL3 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
FCHSD2O948682EBI-704635,EBI-1215612From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248269, 10 interactors
CORUMiQ62915
ELMiQ62915
IntActiQ62915, 8 interactors
MINTiQ62915
STRINGi10116.ENSRNOP00000004187

Structurei

Secondary structure

1909
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ62915
SMRiQ62915
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62915

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 276Protein kinasePROSITE-ProRule annotationAdd BLAST265
Domaini343 – 398L27 1PROSITE-ProRule annotationAdd BLAST56
Domaini402 – 455L27 2PROSITE-ProRule annotationAdd BLAST54
Domaini489 – 564PDZPROSITE-ProRule annotationAdd BLAST76
Domaini600 – 670SH3PROSITE-ProRule annotationAdd BLAST71
Domaini722 – 894Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST173

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni305 – 315Calmodulin-bindingAdd BLAST11

Domaini

The first L27 domain binds DLG1 and the second L27 domain probably binds LIN7.By similarity
The protein kinase domain mediates the interaction with FCHSD2.By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.Curated
Belongs to the MAGUK family.Curated

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG0609 Eukaryota
COG0194 LUCA
HOGENOMiHOG000233034
HOVERGENiHBG001858
InParanoidiQ62915
KOiK06103
PhylomeDBiQ62915

Family and domain databases

CDDicd12081 SH3_CASK, 1 hit
InterProiView protein in InterPro
IPR035473 CASK_SH3
IPR008145 GK/Ca_channel_bsu
IPR008144 Guanylate_kin-like_dom
IPR020590 Guanylate_kinase_CS
IPR011009 Kinase-like_dom_sf
IPR014775 L27_C
IPR004172 L27_dom
IPR036892 L27_dom_sf
IPR027417 P-loop_NTPase
IPR001478 PDZ
IPR036034 PDZ_sf
IPR000719 Prot_kinase_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00625 Guanylate_kin, 1 hit
PF02828 L27, 2 hits
PF00595 PDZ, 1 hit
PF00069 Pkinase, 1 hit
PF07653 SH3_2, 1 hit
SMARTiView protein in SMART
SM00072 GuKc, 1 hit
SM00569 L27, 2 hits
SM00228 PDZ, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF101288 SSF101288, 2 hits
SSF50044 SSF50044, 1 hit
SSF50156 SSF50156, 1 hit
SSF52540 SSF52540, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00856 GUANYLATE_KINASE_1, 1 hit
PS50052 GUANYLATE_KINASE_2, 1 hit
PS51022 L27, 2 hits
PS50106 PDZ, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS50002 SH3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q62915-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADDDVLFED VYELCEVIGK GPFSVVRRCI NRETGQQFAV KIVDVAKFTS
60 70 80 90 100
SPGLSTEDLK REASICHMLK HPHIVELLET YSSDGMLYMV FEFMDGADLC
110 120 130 140 150
FEIVKRADAG FVYSEAVASH YMRQILEALR YCHDNNIIHR DVKPHCVLLA
160 170 180 190 200
SKENSAPVKL GGFGVAIQLG ESGLVAGGRV GTPHFMAPEV VKREPYGKPV
210 220 230 240 250
DVWGCGVILF ILLSGCLPFY GTKERLFEGI IKGKYKMNPR QWSHISESAK
260 270 280 290 300
DLVRRMLMLD PAERITVYEA LNHPWLKERD RYAYKIHLPE TVEQLRKFNA
310 320 330 340 350
RRKLKGAVLA AVSSHKFNSF YGDPPEELPD FSEDPTSSGL LAAERAVSQV
360 370 380 390 400
LDSLEEIHAL TDCSEKDLDF LHSVFQDQHL HTLLDLYDKI NTKSSPQIRN
410 420 430 440 450
PPSDAVQRAK EVLEEISCYP ENNDAKELKR ILTQPHFMAL LQTHHVVAHE
460 470 480 490 500
VYSDEALRVT PPPTSPYLNG DSPESANGDM DMENVTRVRL VQFQKNTDEP
510 520 530 540 550
MGITLKMNEL NHCIVARIMH GGMIHRQGTL HVGDEIREIN GISVANQTVE
560 570 580 590 600
QLQKMLREMR GSITFKIVPS YRTQSSSCER DSPSTSRQSP ANGHSSTNNS
610 620 630 640 650
VSIYVRAQFE YDPAKDDLIP CKEAGIRFRV GDIIQIISKD DHNWWQGKLE
660 670 680 690 700
NSKNGTAGLI PSPELQEWRV ACIAMEKTKQ EQQASCTWFG KKKKQYKDKY
710 720 730 740 750
LAKHNADLVT YEEVVKLPAF KRKTLVLLGA HGVGRRHIKN TLITKHPERF
760 770 780 790 800
AYPIPHTTRP PKKDEENGKN YYFVSHDQMM QDISNNEYLE YGSHEDAMYG
810 820 830 840 850
TKLETIRKIH EQGLIAILDV EPQALKVLRT AEFPPFVVFI AAPTITPGLN
860 870 880 890 900
EDESLQRLQK ESDVLQRTYA HYFDLTIINN EIDETIRHLE EAVELVCTAP

QWVPVSWVY
Length:909
Mass (Da):103,259
Last modified:November 1, 1997 - v1
Checksum:i3694A9A44AC29629
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2K2L8A0A0G2K2L8_RAT
Peripheral plasma membrane protein ...
LOC100910506
909Annotation score:
A0A0G2JVI6A0A0G2JVI6_RAT
Peripheral plasma membrane protein ...
Cask LOC100910506
770Annotation score:
A0A0G2K8F4A0A0G2K8F4_RAT
Peripheral plasma membrane protein ...
Cask LOC100910506
338Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47110 mRNA Translation: AAB19127.1
RefSeqiNP_071520.1, NM_022184.1
UniGeneiRn.72627

Genome annotation databases

GeneIDi29647
KEGGirno:29647
UCSCiRGD:62004 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47110 mRNA Translation: AAB19127.1
RefSeqiNP_071520.1, NM_022184.1
UniGeneiRn.72627

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RSONMR-B/D339-394[»]
ProteinModelPortaliQ62915
SMRiQ62915
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248269, 10 interactors
CORUMiQ62915
ELMiQ62915
IntActiQ62915, 8 interactors
MINTiQ62915
STRINGi10116.ENSRNOP00000004187

PTM databases

iPTMnetiQ62915
PhosphoSitePlusiQ62915
SwissPalmiQ62915

Proteomic databases

PaxDbiQ62915
PRIDEiQ62915

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29647
KEGGirno:29647
UCSCiRGD:62004 rat

Organism-specific databases

CTDi8573
RGDi62004 Cask

Phylogenomic databases

eggNOGiKOG0609 Eukaryota
COG0194 LUCA
HOGENOMiHOG000233034
HOVERGENiHBG001858
InParanoidiQ62915
KOiK06103
PhylomeDBiQ62915

Enzyme and pathway databases

BRENDAi2.7.11.1 5301

Miscellaneous databases

EvolutionaryTraceiQ62915
PROiPR:Q62915

Family and domain databases

CDDicd12081 SH3_CASK, 1 hit
InterProiView protein in InterPro
IPR035473 CASK_SH3
IPR008145 GK/Ca_channel_bsu
IPR008144 Guanylate_kin-like_dom
IPR020590 Guanylate_kinase_CS
IPR011009 Kinase-like_dom_sf
IPR014775 L27_C
IPR004172 L27_dom
IPR036892 L27_dom_sf
IPR027417 P-loop_NTPase
IPR001478 PDZ
IPR036034 PDZ_sf
IPR000719 Prot_kinase_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00625 Guanylate_kin, 1 hit
PF02828 L27, 2 hits
PF00595 PDZ, 1 hit
PF00069 Pkinase, 1 hit
PF07653 SH3_2, 1 hit
SMARTiView protein in SMART
SM00072 GuKc, 1 hit
SM00569 L27, 2 hits
SM00228 PDZ, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF101288 SSF101288, 2 hits
SSF50044 SSF50044, 1 hit
SSF50156 SSF50156, 1 hit
SSF52540 SSF52540, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00856 GUANYLATE_KINASE_1, 1 hit
PS50052 GUANYLATE_KINASE_2, 1 hit
PS51022 L27, 2 hits
PS50106 PDZ, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS50002 SH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCSKP_RAT
AccessioniPrimary (citable) accession number: Q62915
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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