UniProtKB - Q62888 (NLGN2_RAT)
Neuroligin-2
Nlgn2
Functioni
GO - Molecular functioni
- cell adhesion molecule binding Source: BHF-UCL
- identical protein binding Source: RGD
- neurexin family protein binding Source: BHF-UCL
- signaling receptor activity Source: GO_Central
GO - Biological processi
- brain development Source: RGD
- chemical synaptic transmission Source: GO_Central
- gephyrin clustering involved in postsynaptic density assembly Source: BHF-UCL
- inhibitory synapse assembly Source: RGD
- insulin metabolic process Source: RGD
- jump response Source: BHF-UCL
- locomotory exploration behavior Source: BHF-UCL
- modulation of chemical synaptic transmission Source: BHF-UCL
- neuromuscular process controlling balance Source: BHF-UCL
- neuron cell-cell adhesion Source: BHF-UCL
- positive regulation of cell population proliferation Source: RGD
- positive regulation of dendritic spine development Source: RGD
- positive regulation of excitatory postsynaptic potential Source: BHF-UCL
- positive regulation of inhibitory postsynaptic potential Source: BHF-UCL
- positive regulation of insulin secretion Source: RGD
- positive regulation of protein localization to synapse Source: RGD
- positive regulation of synapse assembly Source: BHF-UCL
- positive regulation of synaptic transmission, GABAergic Source: BHF-UCL
- positive regulation of synaptic transmission, glutamatergic Source: BHF-UCL
- positive regulation of synaptic vesicle clustering Source: RGD
- positive regulation of t-SNARE clustering Source: RGD
- postsynaptic density protein 95 clustering Source: BHF-UCL
- postsynaptic membrane assembly Source: BHF-UCL
- postsynaptic specialization assembly Source: RGD
- presynaptic membrane assembly Source: BHF-UCL
- protein localization to synapse Source: BHF-UCL
- regulation of AMPA receptor activity Source: BHF-UCL
- regulation of presynapse assembly Source: SynGO
- regulation of respiratory gaseous exchange by nervous system process Source: BHF-UCL
- sensory perception of pain Source: BHF-UCL
- social behavior Source: BHF-UCL
- synapse assembly Source: BHF-UCL
- synapse organization Source: MGI
- synaptic vesicle endocytosis Source: GO_Central
- terminal button organization Source: BHF-UCL
- thigmotaxis Source: BHF-UCL
Keywordsi
Biological process | Cell adhesion |
Enzyme and pathway databases
Reactomei | R-RNO-6794361, Neurexins and neuroligins |
Protein family/group databases
ESTHERi | ratno-2neur, Neuroligin |
MEROPSi | S09.995 |
Names & Taxonomyi
Protein namesi | Recommended name: Neuroligin-2 |
Gene namesi | Name:Nlgn2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621118, Nlgn2 |
Subcellular locationi
Plasma membrane
Note: Detected at presynaptic membranes in retina, and at postsynaptic membranes in brain. Detected at dendritic spines in cultured neurons. Colocalizes with GPHN and ARHGEF9 at neuronal cell membranes.
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- integral component of postsynaptic membrane Source: BHF-UCL
- integral component of postsynaptic specialization membrane Source: SynGO
- plasma membrane Source: BHF-UCL
- postsynaptic membrane Source: RGD
- presynaptic membrane Source: UniProtKB-SubCell
Other locations
- cell surface Source: BHF-UCL
- cytoplasm Source: RGD
- dendritic shaft Source: RGD
- dopaminergic synapse Source: RGD
- excitatory synapse Source: RGD
- GABA-ergic synapse Source: SynGO
- glycinergic synapse Source: RGD
- inhibitory synapse Source: BHF-UCL
- ribbon synapse Source: RGD
- synapse Source: BHF-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 15 – 678 | ExtracellularSequence analysisAdd BLAST | 664 | |
Transmembranei | 679 – 699 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 700 – 836 | CytoplasmicSequence analysisAdd BLAST | 137 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 770 | Y → A: Abolishes interaction with GPHN. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 14 | 1 PublicationAdd BLAST | 14 | |
ChainiPRO_0000008644 | 15 – 836 | Neuroligin-2Add BLAST | 822 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 98 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 106 ↔ 141 | By similarity | ||
Glycosylationi | 136 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 317 ↔ 328 | By similarity | ||
Disulfide bondi | 487 ↔ 521 | By similarity | ||
Glycosylationi | 522 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 714 | PhosphoserineCombined sources | 1 | |
Modified residuei | 719 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q62888 |
PRIDEi | Q62888 |
PTM databases
GlyGeni | Q62888, 3 sites |
iPTMneti | Q62888 |
PhosphoSitePlusi | Q62888 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Interacts with NRXN1, NRXN2 and NRXN3 (PubMed:8576240, PubMed:18334216, PubMed:20624592).
Interacts (via its C-terminus) with DLG4/PSD-95 (via PDZ domain 3) (By similarity).
Interacts with PATJ (PubMed:9647694).
Interacts with GPHN (PubMed:19755106).
Interacts with MDGA1 and MDGA2 (PubMed:23248271).
Found in a complex with MAGI2 and IGSF9B, where it interacts with MAGI2 (via WW 1, WW 2 and PDZ 2 domains) (PubMed:23751499).
Identified in a complex of 720 kDa composed of LHFPL4, NLGN2, GABRA1, GABRB2, GABRG2 and GABRB3 (PubMed:28279354).
Interacts with LHFPL4; leading to mutual regulation of the protein level and synaptic clustering (PubMed:29742426, PubMed:28279354).
Interacts with GABRA1 (PubMed:28279354).
By similarity9 PublicationsGO - Molecular functioni
- cell adhesion molecule binding Source: BHF-UCL
- identical protein binding Source: RGD
- neurexin family protein binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 250669, 13 interactors |
CORUMi | Q62888 |
IntActi | Q62888, 4 interactors |
MINTi | Q62888 |
STRINGi | 10116.ENSRNOP00000053713 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 679 – 699 | Required for interaction with LHFPL4By similarityAdd BLAST | 21 |
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1516, Eukaryota |
InParanoidi | Q62888 |
OrthoDBi | 754103at2759 |
PhylomeDBi | Q62888 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR002018, CarbesteraseB IPR019819, Carboxylesterase_B_CS IPR000460, Nlgn IPR030023, NLGN2 |
PANTHERi | PTHR43903:SF3, PTHR43903:SF3, 1 hit |
Pfami | View protein in Pfam PF00135, COesterase, 1 hit |
PRINTSi | PR01090, NEUROLIGIN |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00941, CARBOXYLESTERASE_B_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MWLLALCLVG LAGAQRGGGG PGGGAPGGPG LGLGSLGEER FPVVNTAYGR
60 70 80 90 100
VRGVRRELNN EILGPVVQFL GVPYATPPLG ARRFQPPEAP ASWPGVRNAT
110 120 130 140 150
TLPPACPQNL HGALPAIMLP VWFTDNLEAA ATYVQNQSED CLYLNLYVPT
160 170 180 190 200
EDGPLTKKRD EATLNPPDTD IRDSGKKPVM LFLHGGSYME GTGNMFDGSV
210 220 230 240 250
LAAYGNVIVA TLNYRLGVLG FLSTGDQAAK GNYGLLDQIQ ALRWLSENIA
260 270 280 290 300
HFGGDPERIT IFGSGAGASC VNLLILSHHS EGLFQKAIAQ SGTAISSWSV
310 320 330 340 350
NYQPLKYTRL LAAKVGCDRE DSTEAVECLR RKSSRELVDQ DVQPARYHIA
360 370 380 390 400
FGPVVDGDVV PDDPEILMQQ GEFLNYDMLI GVNQGEGLKF VEDSAESEDG
410 420 430 440 450
VSASAFDFTV SNFVDNLYGY PEGKDVLRET IKFMYTDWAD RDNGEMRRKT
460 470 480 490 500
LLALFTDHQW VAPAVATAKL HADYQSPVYF YTFYHHCQAE GRPEWADAAH
510 520 530 540 550
GDELPYVFGV PMVGATDLFP CNFSKNDVML SAVVMTYWTN FAKTGDPNQP
560 570 580 590 600
VPQDTKFIHT KPNRFEEVVW SKFNSKEKQY LHIGLKPRVR DNYRANKVAF
610 620 630 640 650
WLELVPHLHN LHTELFTTTT RLPPYATRWP PRTPGPGTSG TRRPPPPATL
660 670 680 690 700
PPESDIDLGP RAYDRFPGDS RDYSTELSVT VAVGASLLFL NILAFAALYY
710 720 730 740 750
KRDRRQELRC RRLSPPGGSG SGVPGGGPLL PTAGRELPPE EELVSLQLKR
760 770 780 790 800
GGGVGADPAE ALRPACPPDY TLALRRAPDD VPLLAPGALT LLPSGLGPPP
810 820 830
PPPPPSLHPF GPFPPPPPTA TSHNNTLPHP HSTTRV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0U1RRX0 | A0A0U1RRX0_RAT | Neuroligin-2 | Nlgn2 | 876 | Annotation score: | ||
F1LQ41 | F1LQ41_RAT | Neuroligin-2 | Nlgn2 | 834 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_007533 | 153 – 169 | Missing in isoform 2. 1 PublicationAdd BLAST | 17 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U41662 mRNA Translation: AAA97870.1 |
RefSeqi | NP_446444.1, NM_053992.1 [Q62888-1] |
Genome annotation databases
GeneIDi | 117096 |
KEGGi | rno:117096 |
UCSCi | RGD:621118, rat [Q62888-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U41662 mRNA Translation: AAA97870.1 |
RefSeqi | NP_446444.1, NM_053992.1 [Q62888-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5V5V | X-ray | 4.11 | A/B/C/D/E/F | 42-612 | [»] | |
SMRi | Q62888 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 250669, 13 interactors |
CORUMi | Q62888 |
IntActi | Q62888, 4 interactors |
MINTi | Q62888 |
STRINGi | 10116.ENSRNOP00000053713 |
Protein family/group databases
ESTHERi | ratno-2neur, Neuroligin |
MEROPSi | S09.995 |
PTM databases
GlyGeni | Q62888, 3 sites |
iPTMneti | Q62888 |
PhosphoSitePlusi | Q62888 |
Proteomic databases
PaxDbi | Q62888 |
PRIDEi | Q62888 |
Protocols and materials databases
ABCDi | Q62888, 1 sequenced antibody |
Genome annotation databases
GeneIDi | 117096 |
KEGGi | rno:117096 |
UCSCi | RGD:621118, rat [Q62888-1] |
Organism-specific databases
CTDi | 57555 |
RGDi | 621118, Nlgn2 |
Phylogenomic databases
eggNOGi | KOG1516, Eukaryota |
InParanoidi | Q62888 |
OrthoDBi | 754103at2759 |
PhylomeDBi | Q62888 |
Enzyme and pathway databases
Reactomei | R-RNO-6794361, Neurexins and neuroligins |
Miscellaneous databases
PROi | PR:Q62888 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR002018, CarbesteraseB IPR019819, Carboxylesterase_B_CS IPR000460, Nlgn IPR030023, NLGN2 |
PANTHERi | PTHR43903:SF3, PTHR43903:SF3, 1 hit |
Pfami | View protein in Pfam PF00135, COesterase, 1 hit |
PRINTSi | PR01090, NEUROLIGIN |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00941, CARBOXYLESTERASE_B_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NLGN2_RAT | |
Accessioni | Q62888Primary (citable) accession number: Q62888 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 23, 2003 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families