UniProtKB - Q62847 (ADDG_RAT)
Protein
Gamma-adducin
Gene
Add3
Organism
Rattus norvegicus (Rat)
Status
Functioni
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Plays a role in actin filament capping. Binds to calmodulin.By similarity
GO - Molecular functioni
- actin filament binding Source: GO_Central
- calmodulin binding Source: UniProtKB-KW
- protein kinase C binding Source: RGD
- spectrin binding Source: GO_Central
- structural constituent of cytoskeleton Source: RGD
- structural molecule activity Source: GO_Central
GO - Biological processi
- actin filament bundle assembly Source: GO_Central
- barbed-end actin filament capping Source: GO_Central
- positive regulation of vasoconstriction Source: RGD
- response to drug Source: RGD
Keywordsi
Molecular function | Actin-binding, Calmodulin-binding |
Enzyme and pathway databases
Reactomei | R-RNO-5223345, Miscellaneous transport and binding events |
Names & Taxonomyi
Protein namesi | Recommended name: Gamma-adducinAlternative name(s): Adducin-like protein 70 Protein kinase C-binding protein 35H |
Gene namesi | Name:Add3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2043, Add3 |
Subcellular locationi
Plasma membrane
Cytoskeleton
Cytoskeleton
- cytoskeleton Source: RGD
Nucleus
- condensed nuclear chromosome Source: RGD
Plasma Membrane
- plasma membrane raft Source: GO_Central
Other locations
- brush border Source: RGD
- cell cortex Source: RGD
- cell-cell junction Source: RGD
- cytoplasm Source: RGD
- membrane Source: RGD
- postsynaptic density Source: RGD
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000218538 | 2 – 705 | Gamma-adducinAdd BLAST | 704 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 31 | PhosphoserineBy similarity | 1 | |
Modified residuei | 42 | PhosphoserineBy similarity | 1 | |
Modified residuei | 64 | PhosphoserineBy similarity | 1 | |
Modified residuei | 402 | PhosphoserineBy similarity | 1 | |
Modified residuei | 414 | PhosphoserineBy similarity | 1 | |
Modified residuei | 423 | PhosphoserineCombined sources | 1 | |
Modified residuei | 442 | PhosphoserineBy similarity | 1 | |
Modified residuei | 461 | PhosphoserineBy similarity | 1 | |
Cross-linki | 484 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 583 | PhosphoserineCombined sources | 1 | |
Modified residuei | 585 | PhosphoserineCombined sources | 1 | |
Modified residuei | 590 | PhosphoserineBy similarity | 1 | |
Modified residuei | 672 | PhosphoserineCombined sources | 1 | |
Modified residuei | 676 | PhosphoserineCombined sources | 1 | |
Modified residuei | 678 | PhosphoserineBy similarity | 1 | |
Modified residuei | 680 | PhosphoserineCombined sources | 1 | |
Modified residuei | 682 | Phosphoserine; by PKCBy similarity | 1 |
Post-translational modificationi
Sumoylated.By similarity
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q62847 |
PaxDbi | Q62847 |
PRIDEi | Q62847 |
PTM databases
iPTMneti | Q62847 |
PhosphoSitePlusi | Q62847 |
Interactioni
Subunit structurei
Heterodimer of an alpha and a gamma subunit.
1 PublicationGO - Molecular functioni
- actin filament binding Source: GO_Central
- calmodulin binding Source: UniProtKB-KW
- protein kinase C binding Source: RGD
- spectrin binding Source: GO_Central
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000043399 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 683 – 700 | Interaction with calmodulinSequence analysisAdd BLAST | 18 |
Domaini
Comprised of three regions: a N-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3699, Eukaryota |
InParanoidi | Q62847 |
OrthoDBi | 400524at2759 |
PhylomeDBi | Q62847 |
Family and domain databases
Gene3Di | 3.40.225.10, 1 hit |
InterProi | View protein in InterPro IPR027772, ADD3 IPR001303, Aldolase_II/adducin_N IPR036409, Aldolase_II/adducin_N_sf |
PANTHERi | PTHR10672:SF5, PTHR10672:SF5, 1 hit |
Pfami | View protein in Pfam PF00596, Aldolase_II, 1 hit |
SMARTi | View protein in SMART SM01007, Aldolase_II, 1 hit |
SUPFAMi | SSF53639, SSF53639, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketNote: Additional isoforms seem to exist.
This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 2 (identifier: Q62847-1) [UniParc]FASTAAdd to basket
Also known as: Long
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSDSSQAVI TTPPPPSMPH KERYFDRINE SDPEYLRERN MSPDLREDFN
60 70 80 90 100
MMEQRKRVTQ ILQSPAFRED LECLTQEQMK KGHDPTGLLA LQQIADYIMA
110 120 130 140 150
NSFTGFSSPP LSLGMVTPIN DLPGADTSSY VKGEKLTRCE LASLYRLADL
160 170 180 190 200
FGWAHLANTY ISVRVSKEQD HIIIIPRGLP FSEATASALV KVNIIGEVVD
210 220 230 240 250
QGSTNLKIDH SGFSPHAAIY STRPDVKCVI HIHTLATAAV SSMKCGILPI
260 270 280 290 300
SQESLILGDV AYYDYQGSLD EEEERIELQK VLGPSCKVLV LRNHGVVALG
310 320 330 340 350
ETLEEAFHYI FNVQMACEIQ VQAVAGAGGV DNLLILDLQK YKAFTHGVAM
360 370 380 390 400
TGGGGVNMGS HQKWKVGEIE FEGLMRTLDN LGYRTGYAYR HPLVREKPRH
410 420 430 440 450
KSDVEIPATV TAFSFEDDSV PLSPLKYMAQ RQQREKTRWL NSPNTYMKVN
460 470 480 490 500
VPEESRNGET SPRTKITWMK AEDPSKVSSG TPIKIEDPNQ FVPLNTNPTE
510 520 530 540 550
VLEKRNKIRE QNRYDLKTAG PQSQLLAGIV VDKPPSTMRF EDDDQGPPAP
560 570 580 590 600
PNPFSHLMEG ELEEYTKTIE RQQQGLDDAE QESLSDDAAS VSQIQSQTQS
610 620 630 640 650
PQSVPERLEE NHELFSKSFT SVDVPVIVNG KDEMHDVEDE LAQRVSRLTT
660 670 680 690 700
STTIENIEIT IKSPDRTEEV LSPDGSPSKS PSKKKKKFRT PSFLKKNKKK
EKVEA
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V9D7 | G3V9D7_RAT | Adducin 3 (Gamma), isoform CRA_a | Add3 rCG_57421 | 705 | Annotation score: | ||
D3ZCH7 | D3ZCH7_RAT | Adducin 3 (Gamma), isoform CRA_b | Add3 rCG_57421 | 673 | Annotation score: | ||
A0A0G2JW28 | A0A0G2JW28_RAT | Gamma-adducin | Add3 | 670 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 701 – 705 | EKVEA → GES in AAC52277 (PubMed:7592723).Curated | 5 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 572 | Q → K in strain: Milan hypersensitive. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000190 | 578 – 609 | Missing in isoform 1. 1 PublicationAdd BLAST | 32 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U35775 mRNA Translation: AAC52277.1 |
RefSeqi | NP_001157575.1, NM_001164103.1 NP_113740.2, NM_031552.2 |
Genome annotation databases
GeneIDi | 25230 |
KEGGi | rno:25230 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U35775 mRNA Translation: AAC52277.1 |
RefSeqi | NP_001157575.1, NM_001164103.1 NP_113740.2, NM_031552.2 |
3D structure databases
SMRi | Q62847 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000043399 |
PTM databases
iPTMneti | Q62847 |
PhosphoSitePlusi | Q62847 |
Proteomic databases
jPOSTi | Q62847 |
PaxDbi | Q62847 |
PRIDEi | Q62847 |
Genome annotation databases
GeneIDi | 25230 |
KEGGi | rno:25230 |
Organism-specific databases
CTDi | 120 |
RGDi | 2043, Add3 |
Phylogenomic databases
eggNOGi | KOG3699, Eukaryota |
InParanoidi | Q62847 |
OrthoDBi | 400524at2759 |
PhylomeDBi | Q62847 |
Enzyme and pathway databases
Reactomei | R-RNO-5223345, Miscellaneous transport and binding events |
Miscellaneous databases
PROi | PR:Q62847 |
Family and domain databases
Gene3Di | 3.40.225.10, 1 hit |
InterProi | View protein in InterPro IPR027772, ADD3 IPR001303, Aldolase_II/adducin_N IPR036409, Aldolase_II/adducin_N_sf |
PANTHERi | PTHR10672:SF5, PTHR10672:SF5, 1 hit |
Pfami | View protein in Pfam PF00596, Aldolase_II, 1 hit |
SMARTi | View protein in SMART SM01007, Aldolase_II, 1 hit |
SUPFAMi | SSF53639, SSF53639, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ADDG_RAT | |
Accessioni | Q62847Primary (citable) accession number: Q62847 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | December 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 137 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families