Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hyaluronidase PH-20

Gene

Spam1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid (By similarity).By similarity

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei147Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCell adhesion

Enzyme and pathway databases

ReactomeiR-RNO-2534343 Interaction With Cumulus Cells

Protein family/group databases

CAZyiGH56 Glycoside Hydrolase Family 56

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase PH-20 (EC:3.2.1.35)
Short name:
Hyal-PH20
Alternative name(s):
Hyaluronoglucosaminidase PH-20
Sperm adhesion molecule 1
Sperm surface antigen 2B1
Sperm surface protein PH-20
Gene namesi
Name:Spam1
Synonyms:Ph20, Spam
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi620547 Spam1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_0000012098? – 512Removed in mature formSequence analysis
Signal peptidei1 – 35By similarityAdd BLAST35
ChainiPRO_000001209736 – ?Hyaluronidase PH-20

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi60 ↔ 351By similarity
Glycosylationi63N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi165N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi179N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi223 ↔ 237By similarity
Glycosylationi368N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi376 ↔ 387By similarity
Disulfide bondi381 ↔ 435By similarity
Glycosylationi408N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi437 ↔ 464By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ62803
PRIDEiQ62803

Expressioni

Gene expression databases

BgeeiENSRNOG00000007231 Expressed in 2 organ(s), highest expression level in testis
GenevisibleiQ62803 RN

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009537

Structurei

3D structure databases

ProteinModelPortaliQ62803
SMRiQ62803
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410YDEI LUCA
GeneTreeiENSGT00550000074476
HOGENOMiHOG000015133
HOVERGENiHBG052053
InParanoidiQ62803
KOiK01197
OMAiNANTYLC
OrthoDBiEOG091G064G
PhylomeDBiQ62803
TreeFamiTF321598

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR017853 Glycoside_hydrolase_SF
IPR018155 Hyaluronidase
IPR001439 Hyaluronidase_PH20/Hyal5
PANTHERiPTHR11769 PTHR11769, 1 hit
PfamiView protein in Pfam
PF01630 Glyco_hydro_56, 1 hit
PIRSFiPIRSF038193 Hyaluronidase, 1 hit
PIRSF500773 Hyaluronidase_PH20_Hyal5, 1 hit
PRINTSiPR00846 GLHYDRLASE56
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62803-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGELQFKWLF WRSFAESGGT FQTVLIFLFI PYSLTVDYRA TPVLSDTTFV
60 70 80 90 100
WVWNVPTEAC VENVTEPIDL SFFSLIGSPR KTAIGQPVTL FYVDRLGNYP
110 120 130 140 150
HIDAQQTEHH GGIPQKGDLT THLVKAKEDV ERYIPTDKLG LAIIDWEEWR
160 170 180 190 200
PTWMRNWTPK DIYRNKSIEL VQAADPAINI TEATVRAKAQ FEGAAKEFME
210 220 230 240 250
GTLKLGKHIR PKHLWGFYLF PDCYNNKFQV DNYDGQCPDV EKKRNDDLDW
260 270 280 290 300
LWKESTGLYP SVYLKKDLKS SRKATLYVRY RVLESIRVSK VSDESNPVPI
310 320 330 340 350
FVYIRLVFTD HVSEYLLEDD LVNTIGEIVA QGTSGIIIWD AMSLAQRSAG
360 370 380 390 400
CPILRQYMKT TLNPYIVNVT LAAKMCSQTL CKEKGMCSRK TESSDAYLHL
410 420 430 440 450
DPSSFSINVT EAGKYEVLGK PEVKDLEYFS EHFKCSCFSK MTCEETSDMR
460 470 480 490 500
SIQDVNVCMG DNVCIKATLG PNSAFHLLPG KGLLLMTTLA HILHHLPHDI
510
FVFPWKMLVS TP
Length:512
Mass (Da):58,412
Last modified:November 1, 1996 - v1
Checksum:i5ED01DA50F9153B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89999 mRNA Translation: CAA62016.1
BC081748 mRNA Translation: AAH81748.1
RefSeqiNP_446419.1, NM_053967.2
UniGeneiRn.87544

Genome annotation databases

EnsembliENSRNOT00000009537; ENSRNOP00000009537; ENSRNOG00000007231
GeneIDi117037
KEGGirno:117037
UCSCiRGD:620547 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89999 mRNA Translation: CAA62016.1
BC081748 mRNA Translation: AAH81748.1
RefSeqiNP_446419.1, NM_053967.2
UniGeneiRn.87544

3D structure databases

ProteinModelPortaliQ62803
SMRiQ62803
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009537

Protein family/group databases

CAZyiGH56 Glycoside Hydrolase Family 56

Proteomic databases

PaxDbiQ62803
PRIDEiQ62803

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009537; ENSRNOP00000009537; ENSRNOG00000007231
GeneIDi117037
KEGGirno:117037
UCSCiRGD:620547 rat

Organism-specific databases

CTDi6677
RGDi620547 Spam1

Phylogenomic databases

eggNOGiENOG410YDEI LUCA
GeneTreeiENSGT00550000074476
HOGENOMiHOG000015133
HOVERGENiHBG052053
InParanoidiQ62803
KOiK01197
OMAiNANTYLC
OrthoDBiEOG091G064G
PhylomeDBiQ62803
TreeFamiTF321598

Enzyme and pathway databases

ReactomeiR-RNO-2534343 Interaction With Cumulus Cells

Miscellaneous databases

PROiPR:Q62803

Gene expression databases

BgeeiENSRNOG00000007231 Expressed in 2 organ(s), highest expression level in testis
GenevisibleiQ62803 RN

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR017853 Glycoside_hydrolase_SF
IPR018155 Hyaluronidase
IPR001439 Hyaluronidase_PH20/Hyal5
PANTHERiPTHR11769 PTHR11769, 1 hit
PfamiView protein in Pfam
PF01630 Glyco_hydro_56, 1 hit
PIRSFiPIRSF038193 Hyaluronidase, 1 hit
PIRSF500773 Hyaluronidase_PH20_Hyal5, 1 hit
PRINTSiPR00846 GLHYDRLASE56
SUPFAMiSSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHYALP_RAT
AccessioniPrimary (citable) accession number: Q62803
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: November 1, 1996
Last modified: September 12, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again