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UniProtKB - Q62803 (HYALP_RAT)
Protein
Hyaluronidase PH-20
Gene
Spam1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid (By similarity).
By similarityCatalytic activityi
- Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. EC:3.2.1.35
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 147 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- hyalurononglucosaminidase activity Source: RGD
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- cell adhesion Source: RGD
- fusion of sperm to egg plasma membrane involved in single fertilization Source: RGD
- hyaluronan catabolic process Source: GO_Central
- single fertilization Source: RGD
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Cell adhesion |
Protein family/group databases
CAZyi | GH56, Glycoside Hydrolase Family 56 |
Names & Taxonomyi
Protein namesi | Recommended name: Hyaluronidase PH-20 (EC:3.2.1.35)Short name: Hyal-PH20 Alternative name(s): Hyaluronoglucosaminidase PH-20 Sperm adhesion molecule 1 Sperm surface antigen 2B1 Sperm surface protein PH-20 |
Gene namesi | Name:Spam1 Synonyms:Ph20, Spam |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620547, Spam1 |
Subcellular locationi
Plasma membrane
Plasma Membrane
- external side of plasma membrane Source: RGD
- plasma membrane Source: RGD
Other locations
- acrosomal vesicle Source: RGD
- anchored component of membrane Source: UniProtKB-KW
- cytoplasmic vesicle Source: GO_Central
- membrane raft Source: RGD
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 35 | By similarityAdd BLAST | 35 | |
ChainiPRO_0000012097 | 36 – ? | Hyaluronidase PH-20 | ||
PropeptideiPRO_0000012098 | ? – 512 | Removed in mature formSequence analysis |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 60 ↔ 351 | By similarity | ||
Glycosylationi | 63 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 165 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 179 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 223 ↔ 237 | By similarity | ||
Glycosylationi | 368 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 376 ↔ 387 | By similarity | ||
Disulfide bondi | 381 ↔ 435 | By similarity | ||
Glycosylationi | 408 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 437 ↔ 464 | By similarity |
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinProteomic databases
PaxDbi | Q62803 |
PTM databases
GlyGeni | Q62803, 5 sites |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000007231, Expressed in testis and 5 other tissues |
Genevisiblei | Q62803, RN |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 56 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QTUU, Eukaryota |
GeneTreei | ENSGT01020000230364 |
HOGENOMi | CLU_036366_0_1_1 |
InParanoidi | Q62803 |
OMAi | DDQASCE |
OrthoDBi | 1096692at2759 |
PhylomeDBi | Q62803 |
TreeFami | TF321598 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR017853, Glycoside_hydrolase_SF IPR018155, Hyaluronidase IPR001439, Hyaluronidase_PH20/Hyal5 |
PANTHERi | PTHR11769, PTHR11769, 1 hit |
Pfami | View protein in Pfam PF01630, Glyco_hydro_56, 1 hit |
PIRSFi | PIRSF038193, Hyaluronidase, 1 hit PIRSF500773, Hyaluronidase_PH20_Hyal5, 1 hit |
PRINTSi | PR00846, GLHYDRLASE56 |
SUPFAMi | SSF51445, SSF51445, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q62803-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGELQFKWLF WRSFAESGGT FQTVLIFLFI PYSLTVDYRA TPVLSDTTFV
60 70 80 90 100
WVWNVPTEAC VENVTEPIDL SFFSLIGSPR KTAIGQPVTL FYVDRLGNYP
110 120 130 140 150
HIDAQQTEHH GGIPQKGDLT THLVKAKEDV ERYIPTDKLG LAIIDWEEWR
160 170 180 190 200
PTWMRNWTPK DIYRNKSIEL VQAADPAINI TEATVRAKAQ FEGAAKEFME
210 220 230 240 250
GTLKLGKHIR PKHLWGFYLF PDCYNNKFQV DNYDGQCPDV EKKRNDDLDW
260 270 280 290 300
LWKESTGLYP SVYLKKDLKS SRKATLYVRY RVLESIRVSK VSDESNPVPI
310 320 330 340 350
FVYIRLVFTD HVSEYLLEDD LVNTIGEIVA QGTSGIIIWD AMSLAQRSAG
360 370 380 390 400
CPILRQYMKT TLNPYIVNVT LAAKMCSQTL CKEKGMCSRK TESSDAYLHL
410 420 430 440 450
DPSSFSINVT EAGKYEVLGK PEVKDLEYFS EHFKCSCFSK MTCEETSDMR
460 470 480 490 500
SIQDVNVCMG DNVCIKATLG PNSAFHLLPG KGLLLMTTLA HILHHLPHDI
510
FVFPWKMLVS TP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X89999 mRNA Translation: CAA62016.1 BC081748 mRNA Translation: AAH81748.1 |
RefSeqi | NP_446419.1, NM_053967.2 |
Genome annotation databases
Ensembli | ENSRNOT00000009537; ENSRNOP00000009537; ENSRNOG00000007231 |
GeneIDi | 117037 |
KEGGi | rno:117037 |
UCSCi | RGD:620547, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X89999 mRNA Translation: CAA62016.1 BC081748 mRNA Translation: AAH81748.1 |
RefSeqi | NP_446419.1, NM_053967.2 |
3D structure databases
AlphaFoldDBi | Q62803 |
SMRi | Q62803 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000009537 |
Protein family/group databases
CAZyi | GH56, Glycoside Hydrolase Family 56 |
PTM databases
GlyGeni | Q62803, 5 sites |
Proteomic databases
PaxDbi | Q62803 |
Genome annotation databases
Ensembli | ENSRNOT00000009537; ENSRNOP00000009537; ENSRNOG00000007231 |
GeneIDi | 117037 |
KEGGi | rno:117037 |
UCSCi | RGD:620547, rat |
Organism-specific databases
CTDi | 6677 |
RGDi | 620547, Spam1 |
Phylogenomic databases
eggNOGi | ENOG502QTUU, Eukaryota |
GeneTreei | ENSGT01020000230364 |
HOGENOMi | CLU_036366_0_1_1 |
InParanoidi | Q62803 |
OMAi | DDQASCE |
OrthoDBi | 1096692at2759 |
PhylomeDBi | Q62803 |
TreeFami | TF321598 |
Miscellaneous databases
PROi | PR:Q62803 |
Gene expression databases
Bgeei | ENSRNOG00000007231, Expressed in testis and 5 other tissues |
Genevisiblei | Q62803, RN |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR017853, Glycoside_hydrolase_SF IPR018155, Hyaluronidase IPR001439, Hyaluronidase_PH20/Hyal5 |
PANTHERi | PTHR11769, PTHR11769, 1 hit |
Pfami | View protein in Pfam PF01630, Glyco_hydro_56, 1 hit |
PIRSFi | PIRSF038193, Hyaluronidase, 1 hit PIRSF500773, Hyaluronidase_PH20_Hyal5, 1 hit |
PRINTSi | PR00846, GLHYDRLASE56 |
SUPFAMi | SSF51445, SSF51445, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HYALP_RAT | |
Accessioni | Q62803Primary (citable) accession number: Q62803 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2004 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families