UniProtKB - Q62780 (DDX46_RAT)
Protein
Probable ATP-dependent RNA helicase DDX46
Gene
Ddx46
Organism
Rattus norvegicus (Rat)
Status
Functioni
Plays an essential role in splicing, either prior to, or during A complex formation.By similarity
Catalytic activityi
- EC:3.6.4.13
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- RNA binding Source: UniProtKB-KW
- RNA helicase activity Source: UniProtKB-EC
GO - Biological processi
- mRNA splicing, via spliceosome Source: GO_Central
Keywordsi
Molecular function | Helicase, Hydrolase, RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-72163, mRNA Splicing - Major Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Probable ATP-dependent RNA helicase DDX46 (EC:3.6.4.13)Alternative name(s): DEAD box protein 46 Helicase of 117.4 kDa |
Gene namesi | Name:Ddx46 Synonyms:Hel117 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 708480, Ddx46 |
Subcellular locationi
Nucleus
- Nucleus speckle By similarity
- Cajal body By similarity
Other locations
- Membrane By similarity; Lipid-anchor By similarity
Note: Present in Cajal bodies (CBs) and nuclear speckles.By similarity
Nucleus
- Cajal body Source: UniProtKB-SubCell
- fibrillar center Source: Ensembl
- nuclear speck Source: UniProtKB-SubCell
- nucleus Source: RGD
Other locations
- membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000055124 | 2 – 1032 | Probable ATP-dependent RNA helicase DDX46Add BLAST | 1031 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Cross-linki | 186 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 199 | PhosphoserineCombined sources | 1 | |
Modified residuei | 263 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 294 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 295 | PhosphoserineBy similarity | 1 | |
Modified residuei | 296 | PhosphoserineBy similarity | 1 | |
Cross-linki | 325 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 346 | PhosphoserineBy similarity | 1 | |
Modified residuei | 776 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 779 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 804 | PhosphoserineCombined sources | 1 | |
Modified residuei | 904 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 908 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 916 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 929 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Lipoprotein, Myristate, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q62780 |
PaxDbi | Q62780 |
PRIDEi | Q62780 |
PTM databases
iPTMneti | Q62780 |
PhosphoSitePlusi | Q62780 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000021637, Expressed in spleen and 21 other tissues |
Genevisiblei | Q62780, RN |
Interactioni
Subunit structurei
Integral component of the 17S U2 snRNP.
By similarityProtein-protein interaction databases
IntActi | Q62780, 3 interactors |
STRINGi | 10116.ENSRNOP00000030772 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 403 – 581 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 179 | |
Domaini | 592 – 753 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 162 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 152 – 197 | Sequence analysisAdd BLAST | 46 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 372 – 400 | Q motifAdd BLAST | 29 | |
Motifi | 529 – 532 | DEAD box | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 3 – 103 | Arg-richAdd BLAST | 101 | |
Compositional biasi | 202 – 209 | Poly-Asp | 8 | |
Compositional biasi | 297 – 300 | Poly-Glu | 4 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0334, Eukaryota |
GeneTreei | ENSGT00940000157753 |
HOGENOMi | CLU_003041_0_0_1 |
InParanoidi | Q62780 |
OMAi | SKFHSIR |
OrthoDBi | 245118at2759 |
PhylomeDBi | Q62780 |
TreeFami | TF354236 |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q62780-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGRESRHYRK RSASRGRSGS RSRSRSPSDK RSKRGDDRRS RSRDRDRRRE
60 70 80 90 100
RSRSRDKRRS RSRDRKRLRR SRSRERDRSR ERRRSRSRDR RRSRSRSRGR
110 120 130 140 150
RSRSSSPGSK SKKAENRSRS KEKAEGGDSS KEKKKDKDDK EDEKEKDAGN
160 170 180 190 200
FDQNKLEEEM RKRKERVEKW REEQRKKAME NIGELKKEIE EMKQGKKWSL
210 220 230 240 250
EDDDDDEDDP AEAEKEGNEM EDEELDPLDA YMEEVKEEVK KFNMRSVKGG
260 270 280 290 300
AGNEKKSGPT VTKVVTVVTT KKAVVDADKK KGELMENDQD AMEYSSEEEE
310 320 330 340 350
VDLQTALTGY QTKQRKLLEP VDHGKIEYEP FRKNFYVEVP ELAKMSQEEV
360 370 380 390 400
NVFRLEMEGI TVKGKGCPKP IKSWVQCGIS MKILNSLKKH GYEKPTPIQT
410 420 430 440 450
QAIPAIMSGR DLIGIAKTGS GKTIAFLLPM FRHIMDQRSL EEGEGPIAVI
460 470 480 490 500
MTPTRELALQ ITKECKKFSK TLGLRVVCVY GGTGISEQIA ELKRGAEIIV
510 520 530 540 550
CTPGRMIDML AANSGRVTNL RRVTYVVLDE ADRMFDMGFE PQVMRIVDNV
560 570 580 590 600
RPDRQTVMFS ATFPRAMEAL ARRILSKPIE VQVGGRSVVC SDVEQQVIVI
610 620 630 640 650
EEEKKFLKLL ELLGHYQESG SVIIFVDKQE HADGLLKDLM RASYPCMSLH
660 670 680 690 700
GGIDQYDRDS IINDFKNGTC KLLVATSVAA RGLDVKHLIL VVNYSCPNHY
710 720 730 740 750
EDYVHRAGRT GRAGNKGYAY TFITEDQARY AGDIIKALEL SGTAVPPDLE
760 770 780 790 800
KLWSDFKDQQ KAEGKIIKKS SGFSGKGFKF DETEQALANE RKKLQKAALG
810 820 830 840 850
LQDSDDEDAA VDIDEQIESM FNSKKRVKDM AAPGTSSAPA PTAGNAEKLE
860 870 880 890 900
IAKRLALRIN AQKNLGIESQ VDVMQQATNA ILRGGPLLAP TVSAKTIAEQ
910 920 930 940 950
LAEKINAKLN YVPLEKQEEE RQEGGQSESF KRYEEELEIN DFPQTARWKV
960 970 980 990 1000
TSKEALQRIS EYSEAAITIR GTYFPPGKEP KEGERKIYLA IESANELAVQ
1010 1020 1030
KAKAEITRLI KEELIRLQNS YQPTNKGRYK VL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25746 mRNA Translation: AAC52210.1 BC107590 mRNA Translation: AAI07591.1 |
PIRi | A57514 |
RefSeqi | NP_620798.1, NM_139098.1 |
Genome annotation databases
Ensembli | ENSRNOT00000029885; ENSRNOP00000030772; ENSRNOG00000021637 |
GeneIDi | 245957 |
KEGGi | rno:245957 |
UCSCi | RGD:708480, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25746 mRNA Translation: AAC52210.1 BC107590 mRNA Translation: AAI07591.1 |
PIRi | A57514 |
RefSeqi | NP_620798.1, NM_139098.1 |
3D structure databases
SMRi | Q62780 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q62780, 3 interactors |
STRINGi | 10116.ENSRNOP00000030772 |
PTM databases
iPTMneti | Q62780 |
PhosphoSitePlusi | Q62780 |
Proteomic databases
jPOSTi | Q62780 |
PaxDbi | Q62780 |
PRIDEi | Q62780 |
Genome annotation databases
Ensembli | ENSRNOT00000029885; ENSRNOP00000030772; ENSRNOG00000021637 |
GeneIDi | 245957 |
KEGGi | rno:245957 |
UCSCi | RGD:708480, rat |
Organism-specific databases
CTDi | 9879 |
RGDi | 708480, Ddx46 |
Phylogenomic databases
eggNOGi | KOG0334, Eukaryota |
GeneTreei | ENSGT00940000157753 |
HOGENOMi | CLU_003041_0_0_1 |
InParanoidi | Q62780 |
OMAi | SKFHSIR |
OrthoDBi | 245118at2759 |
PhylomeDBi | Q62780 |
TreeFami | TF354236 |
Enzyme and pathway databases
Reactomei | R-RNO-72163, mRNA Splicing - Major Pathway |
Miscellaneous databases
PROi | PR:Q62780 |
Gene expression databases
Bgeei | ENSRNOG00000021637, Expressed in spleen and 21 other tissues |
Genevisiblei | Q62780, RN |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DDX46_RAT | |
Accessioni | Q62780Primary (citable) accession number: Q62780 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 10, 2006 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families