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UniProtKB - Q62773 (S28A2_RAT)
Protein
Sodium/nucleoside cotransporter 2
Gene
Slc28a2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Sodium-dependent and purine-selective. Exhibits the transport characteristics of the nucleoside transport system cif or N1 subtype (N1/cif) (selective for purine nucleosides and uridine). Accepts purine, analogs of purine nucleosides and uridine, and exhibits high affinity for adenosine.
Activity regulationi
Inhibited by formycin B, partially inhibited by purine analog ara-A.
GO - Molecular functioni
- neurotransmitter transmembrane transporter activity Source: RGD
- nucleoside:sodium symporter activity Source: RGD
- nucleoside transmembrane transporter activity Source: RGD
- purine nucleobase transmembrane transporter activity Source: RGD
- purine nucleoside transmembrane transporter activity Source: RGD
- pyrimidine- and adenine-specific:sodium symporter activity Source: RGD
- symporter activity Source: GO_Central
- uridine transmembrane transporter activity Source: RGD
GO - Biological processi
- adenosine transport Source: RGD
- inosine transport Source: RGD
- neurotransmitter transport Source: RGD
- nucleoside transmembrane transport Source: RGD
- protein localization to cell surface Source: RGD
- purine nucleobase transmembrane transport Source: RGD
- purine nucleoside transmembrane transport Source: RGD
- pyrimidine-containing compound transmembrane transport Source: RGD
- retina homeostasis Source: RGD
- uridine transport Source: RGD
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
Reactomei | R-RNO-83936, Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane |
Protein family/group databases
TCDBi | 2.A.41.2.1, the concentrative nucleoside transporter (cnt) family |
Names & Taxonomyi
Protein namesi | Recommended name: Sodium/nucleoside cotransporter 2Alternative name(s): Concentrative nucleoside transporter 2 Short name: CNT 2 Short name: rCNT2 Na(+)/nucleoside cotransporter 2 Sodium-coupled nucleoside transporter 2 Sodium/purine nucleoside cotransporter Short name: SPNT Solute carrier family 28 member 2 |
Gene namesi | Name:Slc28a2 Synonyms:Cnt2, Spnt |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61840, Slc28a2 |
Subcellular locationi
Other locations
Plasma Membrane
- brush border membrane Source: RGD
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: ARUK-UCL
Other locations
- coated vesicle Source: ARUK-UCL
- vesicle membrane Source: ARUK-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 81 – 101 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 105 – 124 | HelicalSequence analysisAdd BLAST | 20 | |
Transmembranei | 149 – 167 | HelicalSequence analysisAdd BLAST | 19 | |
Transmembranei | 173 – 193 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 201 – 221 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 234 – 254 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 261 – 281 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 296 – 315 | HelicalSequence analysisAdd BLAST | 20 | |
Transmembranei | 337 – 356 | HelicalSequence analysisAdd BLAST | 20 | |
Transmembranei | 363 – 382 | HelicalSequence analysisAdd BLAST | 20 | |
Transmembranei | 424 – 444 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 455 – 475 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 530 – 550 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 568 – 588 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000070452 | 1 – 659 | Sodium/nucleoside cotransporter 2Add BLAST | 659 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 46 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q62773 |
PeptideAtlasi | Q62773 |
PRIDEi | Q62773 |
PTM databases
iPTMneti | Q62773 |
PhosphoSitePlusi | Q62773 |
Expressioni
Tissue specificityi
Expressed in liver (in bile canalicular membrane vesicles (CMV) but not in sinusoidal vesicles), jejunum, spleen and heart. Also expressed in brain and skeletal muscle. Not expressed in kidney, muscle and lung.
Interactioni
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000024658 |
Chemistry databases
BindingDBi | Q62773 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 22 | DisorderedSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3747, Eukaryota |
InParanoidi | Q62773 |
OrthoDBi | 471043at2759 |
PhylomeDBi | Q62773 |
Family and domain databases
InterProi | View protein in InterPro IPR008276, C_nuclsd_transpt IPR018270, C_nuclsd_transpt_met_bac IPR030212, CNT1/CNT2 IPR011657, CNT_C_dom IPR002668, CNT_N_dom IPR011642, Gate_dom |
PANTHERi | PTHR10590, PTHR10590, 1 hit PTHR10590:SF11, PTHR10590:SF11, 1 hit |
Pfami | View protein in Pfam PF07670, Gate, 1 hit PF07662, Nucleos_tra2_C, 1 hit PF01773, Nucleos_tra2_N, 1 hit |
TIGRFAMsi | TIGR00804, nupC, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q62773-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKSEGRKSA SQDTSENGME NPGLELMEVG NLEQGKTLEE VTQGHSLKDG
60 70 80 90 100
LGHSSLWRRI LQPFTKARSF YQRHAGLFKK ILLGLLCLAY AAYLLAACIL
110 120 130 140 150
NFRRALALFV ITCLVIFILA CHFLKKFFAK KSIRCLKPLK NTRLRLWLKR
160 170 180 190 200
VFMGAAVVGL ILWLALDTAQ RPEQLISFAG ICMFILILFA CSKHHSAVSW
210 220 230 240 250
RTVFWGLGLQ FVFGILVIRT EPGFNAFQWL GDQIQIFLAY TVEGSSFVFG
260 270 280 290 300
DTLVQSVFAF QSLPIIIFFG CVMSILYYLG LVQWVIQKIA WFLQITMGTT
310 320 330 340 350
AAETLAVAGN IFVGMTEAPL LIRPYLADMT LSEIHAVMTG GFATIAGTVL
360 370 380 390 400
GAFISFGIDA SSLISASVMA APCALALSKL VYPEVEESKF KSKEGVKLPR
410 420 430 440 450
GEERNILEAA SNGATDAIAL VANVAANLIA FLAVLAFINS TLSWLGEMVD
460 470 480 490 500
IHGLTFQVIC SYVLRPMVFM MGVQWADCPL VAEIVGVKFF INEFVAYQQL
510 520 530 540 550
SQYKNKRLSG VEEWINGEKQ WISVKAEIIA TFSLCGFANL TSIGITLGGL
560 570 580 590 600
TSMVPQRKSD LCKLVVRALF TGACVSFISA CMAGILYVPR GAETDCVSFL
610 620 630 640 650
NTNFTNRTYE TYVCCRELFQ STLLNGTNMP SFSGPWQDKE SSLRNLAKCC
DLYTSTVCA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1LN73 | F1LN73_RAT | Sodium/nucleoside cotransporter | Slc28a2 | 659 | Annotation score: | ||
F1LXN6 | F1LXN6_RAT | Sodium/nucleoside cotransporter | Slc28a2 | 659 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 419 | A → G in AAD00159 (PubMed:8967974).Curated | 1 | |
Sequence conflicti | 522 | I → V in AAD00159 (PubMed:8967974).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25055 mRNA Translation: AAA80640.1 U66723 mRNA Translation: AAD00159.1 |
PIRi | A57532 |
RefSeqi | NP_113852.1, NM_031664.1 |
Genome annotation databases
GeneIDi | 60423 |
KEGGi | rno:60423 |
UCSCi | RGD:61840, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25055 mRNA Translation: AAA80640.1 U66723 mRNA Translation: AAD00159.1 |
PIRi | A57532 |
RefSeqi | NP_113852.1, NM_031664.1 |
3D structure databases
SMRi | Q62773 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000024658 |
Chemistry databases
BindingDBi | Q62773 |
ChEMBLi | CHEMBL1287616 |
Protein family/group databases
TCDBi | 2.A.41.2.1, the concentrative nucleoside transporter (cnt) family |
PTM databases
iPTMneti | Q62773 |
PhosphoSitePlusi | Q62773 |
Proteomic databases
PaxDbi | Q62773 |
PeptideAtlasi | Q62773 |
PRIDEi | Q62773 |
Genome annotation databases
GeneIDi | 60423 |
KEGGi | rno:60423 |
UCSCi | RGD:61840, rat |
Organism-specific databases
CTDi | 9153 |
RGDi | 61840, Slc28a2 |
Phylogenomic databases
eggNOGi | KOG3747, Eukaryota |
InParanoidi | Q62773 |
OrthoDBi | 471043at2759 |
PhylomeDBi | Q62773 |
Enzyme and pathway databases
Reactomei | R-RNO-83936, Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane |
Miscellaneous databases
PROi | PR:Q62773 |
Family and domain databases
InterProi | View protein in InterPro IPR008276, C_nuclsd_transpt IPR018270, C_nuclsd_transpt_met_bac IPR030212, CNT1/CNT2 IPR011657, CNT_C_dom IPR002668, CNT_N_dom IPR011642, Gate_dom |
PANTHERi | PTHR10590, PTHR10590, 1 hit PTHR10590:SF11, PTHR10590:SF11, 1 hit |
Pfami | View protein in Pfam PF07670, Gate, 1 hit PF07662, Nucleos_tra2_C, 1 hit PF01773, Nucleos_tra2_N, 1 hit |
TIGRFAMsi | TIGR00804, nupC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | S28A2_RAT | |
Accessioni | Q62773Primary (citable) accession number: Q62773 Secondary accession number(s): Q9QWI3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families