UniProtKB - Q62762 (KC1G2_RAT)
Protein
Casein kinase I isoform gamma-2
Gene
Csnk1g2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation (By similarity).By similarity
Catalytic activityi
Activity regulationi
Stimulated by estrogen.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 75 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 165 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 52 – 60 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein serine/threonine kinase activity Source: RGD
- protein serine kinase activity Source: UniProtKB-EC
- protein threonine kinase activity Source: UniProtKB-EC
GO - Biological processi
- endocytosis Source: GO_Central
- peptidyl-serine phosphorylation Source: RGD
- positive regulation of canonical Wnt signaling pathway Source: GO_Central
- protein autophosphorylation Source: RGD
- protein phosphorylation Source: RGD
- Wnt signaling pathway Source: GO_Central
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Wnt signaling pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 5301 |
Reactomei | R-RNO-1660661, Sphingolipid de novo biosynthesis |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Csnk1g2 Synonyms:Ck1g2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621407, Csnk1g2 |
Subcellular locationi
Other locations
- cell cortex By similarity
- Cytoplasm By similarity
Nucleus
- nucleus Source: GO_Central
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- cell cortex Source: UniProtKB-SubCell
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000192844 | 1 – 415 | Casein kinase I isoform gamma-2Add BLAST | 415 |
Post-translational modificationi
Autophosphorylated (PubMed:7759525). Phosphorylated by aPKC which promotes dissociation from the cell cortex (By similarity).By similarity1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q62762 |
PaxDbi | Q62762 |
PRIDEi | Q62762 |
PTM databases
SwissPalmi | Q62762 |
Expressioni
Tissue specificityi
Testis.
Gene expression databases
Bgeei | ENSRNOG00000018529, Expressed in testis and 21 other tissues |
ExpressionAtlasi | Q62762, baseline and differential |
Genevisiblei | Q62762, RN |
Interactioni
Subunit structurei
Monomer.
Interacts with MTA1 (short isoform) in the cytoplasm.
Interacts with SMAD3 (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000025266 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 46 – 316 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 271 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 369 – 415 | Phospho-regulated basic and hydrophobic (PRBH) motifBy similarityAdd BLAST | 47 |
Domaini
The phospho-regulated basic and hydrophobic (PRBH) motif is sufficient and important for interaction with phospholipids permitting cortical localization (By similarity). Phosphorylation of the PRBH motif by aPKC inhibits the association of the protein with the cortical membrane (By similarity).By similarity
Sequence similaritiesi
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.Curated
Phylogenomic databases
eggNOGi | KOG1165, Eukaryota |
GeneTreei | ENSGT00940000156470 |
HOGENOMi | CLU_019279_2_0_1 |
InParanoidi | Q62762 |
OMAi | HERHFIY |
OrthoDBi | 889559at2759 |
PhylomeDBi | Q62762 |
Family and domain databases
InterProi | View protein in InterPro IPR022247, Casein_kinase-1_gamma_C IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF12605, CK1gamma_C, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q62762-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDFDKKGGKG ELEEGRRMSK TGTNRSNHGV RNSGTSSGVL MVGPNFRVGK
60 70 80 90 100
KIGCGNFGEL RLGKNLYTNE YVAIKLEPIK SRAPQLHLEY RFYKQLSTTE
110 120 130 140 150
GVPQVYYFGP CGKYNAMVLE LLGPSLEDLF DLCDRTFTLK TVLMIAIQLI
160 170 180 190 200
TRMEYVHTKS LIYRDVKPEN FLVGRPGSKR QHSIHIIDFG LAKEYIDPET
210 220 230 240 250
KKHIPYREHK SLTGTARYMS INTHLGKEQS RRDDLEALGH MFMYFLRGSL
260 270 280 290 300
PWQGLKADTL KERYQKIGDT KRATPIEVLC ESFPEEMATY LRYVRRLDFF
310 320 330 340 350
EKPDYDYLRK LFTDLFDRSG YVFDYEYDWA GKPLPTPIGT VHPDVPSQPP
360 370 380 390 400
HRDKAQLHTK NQALNSTNGE LNTDDPTAGH SNAPIAAPAE VEVADETKCC
410
CFFKRRKRKS LQRHK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0H2UHQ2 | A0A0H2UHQ2_RAT | Non-specific serine/threonine prote... | Csnk1g2 rCG_29299 | 414 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 59 – 60 | EL → DV in AAC52201 (PubMed:7759525).Curated | 2 | |
Sequence conflicti | 100 | Missing in AAC52201 (PubMed:7759525).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U22297 mRNA Translation: AAC52201.1 BC072533 mRNA Translation: AAH72533.1 |
PIRi | B56711 |
RefSeqi | NP_001029042.1, NM_001033870.1 NP_075590.2, NM_023102.2 XP_006241064.1, XM_006241002.3 |
Genome annotation databases
Ensembli | ENSRNOT00000080342; ENSRNOP00000072196; ENSRNOG00000018529 |
GeneIDi | 65278 |
KEGGi | rno:65278 |
UCSCi | RGD:621407, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U22297 mRNA Translation: AAC52201.1 BC072533 mRNA Translation: AAH72533.1 |
PIRi | B56711 |
RefSeqi | NP_001029042.1, NM_001033870.1 NP_075590.2, NM_023102.2 XP_006241064.1, XM_006241002.3 |
3D structure databases
SMRi | Q62762 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000025266 |
PTM databases
SwissPalmi | Q62762 |
Proteomic databases
jPOSTi | Q62762 |
PaxDbi | Q62762 |
PRIDEi | Q62762 |
Genome annotation databases
Ensembli | ENSRNOT00000080342; ENSRNOP00000072196; ENSRNOG00000018529 |
GeneIDi | 65278 |
KEGGi | rno:65278 |
UCSCi | RGD:621407, rat |
Organism-specific databases
CTDi | 1455 |
RGDi | 621407, Csnk1g2 |
Phylogenomic databases
eggNOGi | KOG1165, Eukaryota |
GeneTreei | ENSGT00940000156470 |
HOGENOMi | CLU_019279_2_0_1 |
InParanoidi | Q62762 |
OMAi | HERHFIY |
OrthoDBi | 889559at2759 |
PhylomeDBi | Q62762 |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 5301 |
Reactomei | R-RNO-1660661, Sphingolipid de novo biosynthesis |
Miscellaneous databases
PROi | PR:Q62762 |
Gene expression databases
Bgeei | ENSRNOG00000018529, Expressed in testis and 21 other tissues |
ExpressionAtlasi | Q62762, baseline and differential |
Genevisiblei | Q62762, RN |
Family and domain databases
InterProi | View protein in InterPro IPR022247, Casein_kinase-1_gamma_C IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF12605, CK1gamma_C, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KC1G2_RAT | |
Accessioni | Q62762Primary (citable) accession number: Q62762 Secondary accession number(s): Q6IMY5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | October 11, 2004 | |
Last modified: | February 10, 2021 | |
This is version 160 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families