UniProtKB - Q62755 (NR1H2_RAT)
Oxysterols receptor LXR-beta
Nr1h2
Functioni
Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (By similarity).
Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8; DLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity).
Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis (By similarity).
Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators (By similarity).
Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex (By similarity).
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 75 – 152 | Nuclear receptorPROSITE-ProRule annotationAdd BLAST | 78 | |
Zinc fingeri | 78 – 98 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 116 – 140 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- apolipoprotein A-I receptor binding Source: RGD
- ATPase binding Source: RGD
- DNA binding Source: RGD
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: RGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: RGD
- nuclear receptor activity Source: RGD
- retinoid X receptor binding Source: RGD
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- zinc ion binding Source: InterPro
GO - Biological processi
- cell differentiation Source: GO_Central
- cellular lipid metabolic process Source: RGD
- cholesterol homeostasis Source: UniProtKB
- lipid homeostasis Source: RGD
- negative regulation of cholesterol storage Source: RGD
- negative regulation of cold-induced thermogenesis Source: YuBioLab
- negative regulation of gene expression Source: RGD
- negative regulation of lipid transport Source: RGD
- negative regulation of pinocytosis Source: RGD
- negative regulation of proteolysis Source: RGD
- negative regulation of response to endoplasmic reticulum stress Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: RGD
- negative regulation of transcription by RNA polymerase II Source: RGD
- phosphatidylcholine acyl-chain remodeling Source: UniProtKB
- positive regulation of cellular protein metabolic process Source: RGD
- positive regulation of cholesterol efflux Source: RGD
- positive regulation of cholesterol transport Source: RGD
- positive regulation of fatty acid biosynthetic process Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of high-density lipoprotein particle assembly Source: RGD
- positive regulation of lipid storage Source: RGD
- positive regulation of lipoprotein lipase activity Source: RGD
- positive regulation of pancreatic juice secretion Source: RGD
- positive regulation of pri-miRNA transcription by RNA polymerase II Source: Ensembl
- positive regulation of secretion of lysosomal enzymes Source: RGD
- positive regulation of transcription, DNA-templated Source: RGD
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- positive regulation of triglyceride biosynthetic process Source: RGD
- regulation of transcription, DNA-templated Source: RGD
- regulation of transcription by RNA polymerase II Source: RGD
- response to nutrient levels Source: RGD
- retinoic acid receptor signaling pathway Source: RGD
Keywordsi
Molecular function | Activator, DNA-binding, Receptor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-383280, Nuclear Receptor transcription pathway R-RNO-4090294, SUMOylation of intracellular receptors R-RNO-8866427, VLDLR internalisation and degradation R-RNO-9029569, NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux R-RNO-9623433, NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis |
Names & Taxonomyi
Protein namesi | Recommended name: Oxysterols receptor LXR-betaAlternative name(s): Liver X receptor beta Nuclear receptor subfamily 1 group H member 2 Orphan nuclear receptor OR-1 Ubiquitously-expressed nuclear receptor Short name: UR |
Gene namesi | Name:Nr1h2 Synonyms:Lxrb |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61906, Nr1h2 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Nucleus
- nucleus Source: RGD
- RNA polymerase II transcription regulator complex Source: Ensembl
Other locations
- cytoplasm Source: RGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053534 | 1 – 446 | Oxysterols receptor LXR-betaAdd BLAST | 446 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 395 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 433 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | Q62755 |
PRIDEi | Q62755 |
PTM databases
PhosphoSitePlusi | Q62755 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000019812, Expressed in thymus and 21 other tissues |
Genevisiblei | Q62755, RN |
Interactioni
Subunit structurei
Forms a heterodimer with RXR.
Interacts with CCAR2 (via N-terminus) in a ligand-independent manner.
Interacts (when sumoylated) with GPS2; interaction with GPS2 onto hepatic acute phase protein promoters prevents N-Cor corepressor complex dissociation (By similarity).
Interacts with ABCA12 and ABCA1; this interaction is required for ABCA1 localization to the cell surface and is necessary for its normal activity and stability (By similarity).
By similarityGO - Molecular functioni
- apolipoprotein A-I receptor binding Source: RGD
- ATPase binding Source: RGD
- retinoid X receptor binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000026862 |
Chemistry databases
BindingDBi | Q62755 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 208 – 446 | NR LBDPROSITE-ProRule annotationAdd BLAST | 239 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 76 | Transactivation AF-1; required for ligand-independent transactivation functionBy similarityAdd BLAST | 76 | |
Regioni | 1 – 69 | DisorderedSequence analysisAdd BLAST | 69 | |
Regioni | 160 – 201 | DisorderedSequence analysisAdd BLAST | 42 | |
Regioni | 205 – 446 | Transactivation AF-2; required for ligand-dependent transactivation function; mediates interaction with CCAR2By similarityAdd BLAST | 242 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 32 | Polar residuesSequence analysisAdd BLAST | 32 | |
Compositional biasi | 55 – 69 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 179 – 201 | Polar residuesSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 78 – 98 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 116 – 140 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000161465 |
HOGENOMi | CLU_007368_12_4_1 |
InParanoidi | Q62755 |
OMAi | GMREQCE |
OrthoDBi | 1137281at2759 |
PhylomeDBi | Q62755 |
TreeFami | TF352167 |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR023257, Liver_X_rcpt IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR02034, LIVERXRECPTR PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSSPTSSLDT PLPGNGSPQP STSSTSPTIK EEGQETDPPP GSEGSSSAYI
60 70 80 90 100
VVILEPEDEP ERKRKKGPAP KMLGHELCRV CGDKASGFHY NVLSCEGCKG
110 120 130 140 150
FFRRSVVHGG AGRYACRGSG TCQMDAFMRR KCQLCRLRKC KEAGMREQCV
160 170 180 190 200
LSEEQIRKKK IQKQQQQQPP PPTEPASGSS ARPAASPGTS EASSQGSGEG
210 220 230 240 250
EGIQLTAAQE LMIQQLVAAQ LQCNKRSFSD QPKVTPWPLG ADPQSRDARQ
260 270 280 290 300
QRFAHFTELA IISVQEIVDF AKQVPGFLQL GREDQIALLK ASTIEIMLLE
310 320 330 340 350
TARRYNHETE CITFLKDFTY SKDDFHRAGL QVEFINPIFE FSRAMRRLGL
360 370 380 390 400
DDAEYALLIA INIFSADRPN VQEPSRVEAL QQPYVEALLS YTRIKRPQDQ
410 420 430 440
LRFPRMLMKL VSLRTLSSVH SEQVFALRLQ DKKLPPLLSE IWDVHE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 33 | G → V in AAA52361 (PubMed:7971966).Curated | 1 | |
Sequence conflicti | 52 – 54 | Missing in AAA52361 (PubMed:7971966).Curated | 3 | |
Sequence conflicti | 219 | A → V in AAA52361 (PubMed:7971966).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U20389 mRNA Translation: AAA69522.1 U14533 mRNA Translation: AAA52361.1 |
PIRi | I59354 |
RefSeqi | NP_113814.1, NM_031626.1 |
Genome annotation databases
Ensembli | ENSRNOT00000026862; ENSRNOP00000026862; ENSRNOG00000019812 |
GeneIDi | 58851 |
KEGGi | rno:58851 |
UCSCi | RGD:61906, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U20389 mRNA Translation: AAA69522.1 U14533 mRNA Translation: AAA52361.1 |
PIRi | I59354 |
RefSeqi | NP_113814.1, NM_031626.1 |
3D structure databases
SMRi | Q62755 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000026862 |
Chemistry databases
BindingDBi | Q62755 |
ChEMBLi | CHEMBL4105785 |
PTM databases
PhosphoSitePlusi | Q62755 |
Proteomic databases
PaxDbi | Q62755 |
PRIDEi | Q62755 |
Genome annotation databases
Ensembli | ENSRNOT00000026862; ENSRNOP00000026862; ENSRNOG00000019812 |
GeneIDi | 58851 |
KEGGi | rno:58851 |
UCSCi | RGD:61906, rat |
Organism-specific databases
CTDi | 7376 |
RGDi | 61906, Nr1h2 |
Phylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000161465 |
HOGENOMi | CLU_007368_12_4_1 |
InParanoidi | Q62755 |
OMAi | GMREQCE |
OrthoDBi | 1137281at2759 |
PhylomeDBi | Q62755 |
TreeFami | TF352167 |
Enzyme and pathway databases
Reactomei | R-RNO-383280, Nuclear Receptor transcription pathway R-RNO-4090294, SUMOylation of intracellular receptors R-RNO-8866427, VLDLR internalisation and degradation R-RNO-9029569, NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux R-RNO-9623433, NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis |
Miscellaneous databases
PROi | PR:Q62755 |
Gene expression databases
Bgeei | ENSRNOG00000019812, Expressed in thymus and 21 other tissues |
Genevisiblei | Q62755, RN |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR023257, Liver_X_rcpt IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR02034, LIVERXRECPTR PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NR1H2_RAT | |
Accessioni | Q62755Primary (citable) accession number: Q62755 Secondary accession number(s): Q62694 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 173 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families