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UniProtKB - Q62747 (SYT7_RAT)

Protein

Synaptotagmin-7

Gene

Syt7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ca2+ sensor involved in Ca2+-dependent exocytosis of secretory and synaptic vesicles through Ca2+ and phospholipid binding to the C2 domain (PubMed:11395007, PubMed:10725327, PubMed:11511344). Ca2+ induces binding of the C2-domains to phospholipid membranes and to assembled SNARE-complexes; both actions contribute to triggering exocytosis (PubMed:11395007). SYT7 binds Ca2+ with high affinity and slow kinetics compared to other synaptotagmins (By similarity). Involved in Ca2+-triggered lysosomal exocytosis, a major component of the plasma membrane repair (PubMed:10725327, PubMed:11511344). Ca2+-regulated delivery of lysosomal membranes to the cell surface is also involved in the phagocytic uptake of particles by macrophages. Ca2+-triggered lysosomal exocytosis also plays a role in bone remodeling by regulating secretory pathways in osteoclasts and osteoblasts. Involved in cholesterol transport from lysosome to peroxisome by promoting membrane contacts between lysosomes and peroxisomes: probably acts by promoting vesicle fusion by binding phosphatidylinositol-4,5-bisphosphate on peroxisomal membranes. Acts as a key mediator of synaptic facilitation, a process also named short-term synaptic potentiation: synaptic facilitation takes place at synapses with a low initial release probability and is caused by influx of Ca2+ into the axon terminal after spike generation, increasing the release probability of neurotransmitters. Probably mediates synaptic facilitation by directly increasing the probability of release. May also contribute to synaptic facilitation by regulating synaptic vesicle replenishment, a process required to ensure that synaptic vesicles are ready for the arrival of the next action potential: SYT7 is required for synaptic vesicle replenishment by acting as a sensor for Ca2+ and by forming a complex with calmodulin. Also acts as a regulator of Ca2+-dependent insulin and glucagon secretion in beta-cells. Triggers exocytosis by promoting fusion pore opening and fusion pore expansion in chromaffin cells (By similarity). Also regulates the secretion of some non-synaptic secretory granules of specialized cells (PubMed:15456748).By similarity4 Publications

Cofactori

Ca2+1 Publication1 PublicationNote: Binds 3 Ca2+ ions per C2 domain.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi166Calcium 1By similarity1
Metal bindingi166Calcium 2By similarity1
Metal bindingi172Calcium 11 Publication1
Metal bindingi225Calcium 11 Publication1
Metal bindingi225Calcium 21 Publication1
Metal bindingi227Calcium 1By similarity1
Metal bindingi227Calcium 2By similarity1
Metal bindingi227Calcium 3By similarity1
Metal bindingi230Calcium 3By similarity1
Metal bindingi233Calcium 2By similarity1
Metal bindingi233Calcium 3By similarity1
Metal bindingi297Calcium 4By similarity1
Metal bindingi297Calcium 5By similarity1
Metal bindingi303Calcium 5By similarity1
Metal bindingi357Calcium 4By similarity1
Metal bindingi357Calcium 5By similarity1
Metal bindingi359Calcium 4By similarity1
Metal bindingi359Calcium 5By similarity1
Metal bindingi359Calcium 6By similarity1
Metal bindingi362Calcium 6By similarity1
Metal bindingi365Calcium 4By similarity1
Metal bindingi365Calcium 6By similarity1

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: UniProtKB
  • calcium ion binding Source: RGD
  • calmodulin binding Source: UniProtKB
  • clathrin binding Source: BHF-UCL
  • phosphatidylinositol-4,5-bisphosphate binding Source: UniProtKB
  • phosphatidylserine binding Source: Ensembl
  • syntaxin binding Source: BHF-UCL
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionCalmodulin-binding
Biological processExocytosis
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-7Curated
Alternative name(s):
Protein Syt7Imported
Synaptotagmin VII1 Publication
Short name:
SytVII1 Publication
Gene namesi
Name:Syt7Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi62013 Syt7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 16VesicularSequence analysisAdd BLAST16
Transmembranei17 – 37HelicalSequence analysisAdd BLAST21
Topological domaini38 – 403CytoplasmicSequence analysisAdd BLAST366

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane, Peroxisome, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi172D → N: Loss of Ca(2+)-binding in the first C2 domain; when associated with N-225. 1 Publication1
Mutagenesisi225D → N: Loss of Ca(2+)-binding in the first C2 domain; when associated with N-172. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004360551 – 403Synaptotagmin-7Add BLAST403

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52PhosphoserineCombined sources1
Modified residuei58PhosphothreonineCombined sources1
Modified residuei61PhosphoserineCombined sources1
Modified residuei119PhosphoserineCombined sources1
Modified residuei122PhosphoserineCombined sources1

Post-translational modificationi

Palmitoylated at its vesicular N-terminus; palmitoylation is required for localization to lysosome and phagocytosis in macrophages.By similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ62747
PRIDEiQ62747

PTM databases

iPTMnetiQ62747
PhosphoSitePlusiQ62747

Expressioni

Gene expression databases

BgeeiENSRNOG00000026432 Expressed in 10 organ(s), highest expression level in brain

Interactioni

Subunit structurei

Homodimer. Can also form heterodimers with SYT6, SYT9 and SYT10. Interacts with calmodulin (CALM1, CALM2 or CALM3). Interacts with CD63; required for localization to lysosomes.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Stx1aO355262EBI-16179541,EBI-400878From Mus musculus.

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

CORUMiQ62747
DIPiDIP-61710N
IntActiQ62747, 1 interactor
STRINGi10116.ENSRNOP00000031195

Structurei

Secondary structure

1403
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ62747
SMRiQ62747
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini150 – 239C2 1PROSITE-ProRule annotationAdd BLAST90
Domaini280 – 371C2 2PROSITE-ProRule annotationAdd BLAST92

Domaini

The C2 domains bind Ca2+ and membranes (PubMed:11823420). Binding to membranes involves Ca2+-dependent phospholipid binding (PubMed:11823420). Compared to other members of the family, the C2 domains of SYT7 dock and insert into cellular membranes in response to intracellular Ca2+ concentrations that are lower than those required for other synaptotagmins. The two C2 domains bind independently to planar membranes, without interdomain cooperativity. Moreover, SYT7 C2 domains insert more deeply into membranes compared to other synaptotagmins (By similarity).By similarity1 Publication

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410KDM6 Eukaryota
ENOG410XS7N LUCA
GeneTreeiENSGT00760000118973
HOGENOMiHOG000232126
HOVERGENiHBG005010
KOiK19907
OMAiTGEPKCQ
OrthoDBiEOG091G08NG
TreeFamiTF315600

Family and domain databases

CDDicd08386 C2A_Synaptotagmin-7, 1 hit
cd08405 C2B_Synaptotagmin-7, 1 hit
Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR037732 C2A_Synaptotagmin-7
IPR037741 C2B_Synaptotagmin-7
IPR001565 Synaptotagmin
IPR015427 Synaptotagmin7
PANTHERiPTHR10024:SF206 PTHR10024:SF206, 1 hit
PfamiView protein in Pfam
PF00168 C2, 2 hits
PRINTSiPR00360 C2DOMAIN
PR00399 SYNAPTOTAGMN
SMARTiView protein in SMART
SM00239 C2, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits

Sequences (8)i

Sequence statusi: Complete.

This entry describes 8 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q62747-1) [UniParc]FASTAAdd to basket
Also known as: Synaptotagmin VIIs1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MYRDPEAASP GAPTRDVLLV SAIITVSLSV TIVLCGLCHW CQRKLGKRYK 
        60         70         80         90        100
NSLETVGTPD SGRGRGEKKA IKLPAGGKAV NTAPVPGQTP HDESDRRTEP 
       110        120        130        140        150
RSSVSDLVNS LTSEMLMLSP GSEEDEAHEG CSRENLGRIQ FSVGYNFQES 
       160        170        180        190        200
TLTVKVMKAQ ELPAKDFSGT SDPFVKIYLL PDKKHKLETK VKRKNLNPHW 
       210        220        230        240        250
NETFLFEGFP YEKVVQRILY LQVLDYDRFS RNDPIGEVSI PLNKVDLTQM 
       260        270        280        290        300
QTFWKDLKPC SDGSGSRGEL LLSLCYNPSA NSIIVNIIKA RNLKAMDIGG 
       310        320        330        340        350
TSDPYVKVWL MYKDKRVEKK KTVTKKRNLN PIFNESFAFD IPTEKLRETT 
       360        370        380        390        400
IIITVMDKDK LSRNDVIGKI YLSWKSGPGE VKHWKDMIAR PRQPVAQWHQ 

LKA
Note: Major isoform.1 Publication
Length:403
Mass (Da):45,482
Last modified:November 1, 1996 - v1
Checksum:i3153FD7FC1DEEEFB
GO
Isoform 2 (identifier: Q62747-2) [UniParc]FASTAAdd to basket
Also known as: Synaptotagmin VIIL1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     72-72: K → NDLDRDFWNN...QNQNAQGDKR

Show »
Length:687
Mass (Da):75,816
Checksum:i9D085A6E7B3794A1
GO
Isoform 3 (identifier: Q62747-3) [UniParc]FASTAAdd to basket
Also known as: Synaptotagmin VIId1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     72-72: K → NDLDRDFWNN...QNQNAQGDKR

Show »
Length:611
Mass (Da):68,335
Checksum:i3BDC3B272C47F56F
GO
Isoform 4 (identifier: Q62747-4) [UniParc]FASTAAdd to basket
Also known as: Synaptotagmin VIIe1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     72-72: K → NGTLLSGAKV...QNQNAQGDKR

Show »
Length:643
Mass (Da):70,617
Checksum:i5507974FFE5AFDDE
GO
Isoform 5 (identifier: Q62747-5) [UniParc]FASTAAdd to basket
Also known as: Synaptotagmin VIIb1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     72-72: K → NDLDRDFWNN...LAAGKLNLSK

Show »
Length:523
Mass (Da):58,162
Checksum:i78E6D8BE0CBD2DC3
GO
Isoform 6 (identifier: Q62747-6) [UniParc]FASTAAdd to basket
Also known as: Synaptotagmin VIIc1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     72-72: K → NFEDSTLSTA...QNQNAQGDKR

Show »
Length:567
Mass (Da):63,137
Checksum:i4BAF44D0ED872FE1
GO
Isoform 7 (identifier: Q62747-7) [UniParc]FASTAAdd to basket
Also known as: Synaptotagmin VIIa1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     72-72: K → NGTLLSGAKV...QNQNAQGDKR

Show »
Length:520
Mass (Da):57,442
Checksum:i409B9696CD46B502
GO
Isoform 8 (identifier: Q62747-8) [UniParc]FASTAAdd to basket
Also known as: Synaptotagmin VIIT11 Publication, Synaptotagmin VIIT21 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     72-122: KLPAGGKAVN...SEMLMLSPGS → NDLDRDFWNN...LSLNPLFLRR
     123-403: Missing.

Show »
Length:122
Mass (Da):13,768
Checksum:i8AC5E757E3F4E417
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05823872 – 122KLPAG…LSPGS → NDLDRDFWNNNESTVQQKWS SYPPKEFILNISPYAPYGDP RLSLNPLFLRR in isoform 8. Add BLAST51
Alternative sequenceiVSP_05823972K → NDLDRDFWNNNESTVQQKWS SYPPKEFILNISPYAPYGDP RLSLNGTLLSGAKVATAAAG LAVEREGRLGEKPAPVPPPG EDALRSGGAAPSEPGSSGKA GRGRWRMVQSHLAAGKLNLS NFEDSTLSTATTLESIPSSA GEPKCQRPRTLMRQQSLQQP LSQNQRGRQPSQPTTSQSLG QLQAHAASAPGSNPRAYGRG QARQGTSAGSKYRAAGGRSR SNPGSWDHVVGQIRNRGLDM KSFLEGRMVVLSLVLGLSEQ DDFANIPDLQNPGTQQNQNA QGDKR in isoform 2. 1
Alternative sequenceiVSP_05824072K → NDLDRDFWNNNESTVQQKWS SYPPKEFILNISPYAPYGDP RLSLNFEDSTLSTATTLESI PSSAGEPKCQRPRTLMRQQS LQQPLSQNQRGRQPSQPTTS QSLGQLQAHAASAPGSNPRA YGRGQARQGTSAGSKYRAAG GRSRSNPGSWDHVVGQIRNR GLDMKSFLEGRMVVLSLVLG LSEQDDFANIPDLQNPGTQQ NQNAQGDKR in isoform 3. 1
Alternative sequenceiVSP_05824172K → NGTLLSGAKVATAAAGLAVE REGRLGEKPAPVPPPGEDAL RSGGAAPSEPGSSGKAGRGR WRMVQSHLAAGKLNLSNFED STLSTATTLESIPSSAGEPK CQRPRTLMRQQSLQQPLSQN QRGRQPSQPTTSQSLGQLQA HAASAPGSNPRAYGRGQARQ GTSAGSKYRAAGGRSRSNPG SWDHVVGQIRNRGLDMKSFL EGRMVVLSLVLGLSEQDDFA NIPDLQNPGTQQNQNAQGDK R in isoform 4. 1
Alternative sequenceiVSP_05824272K → NDLDRDFWNNNESTVQQKWS SYPPKEFILNISPYAPYGDP RLSLNGTLLSGAKVATAAAG LAVEREGRLGEKPAPVPPPG EDALRSGGAAPSEPGSSGKA GRGRWRMVQSHLAAGKLNLS K in isoform 5. 1
Alternative sequenceiVSP_05824372K → NFEDSTLSTATTLESIPSSA GEPKCQRPRTLMRQQSLQQP LSQNQRGRQPSQPTTSQSLG QLQAHAASAPGSNPRAYGRG QARQGTSAGSKYRAAGGRSR SNPGSWDHVVGQIRNRGLDM KSFLEGRMVVLSLVLGLSEQ DDFANIPDLQNPGTQQNQNA QGDKR in isoform 6. 1
Alternative sequenceiVSP_05824472K → NGTLLSGAKVATAAAGLAVE REGRLGEKPAPVPPPGEDAL RSGGAAPSEPGSSGKAGRGR WRMVQSHLAAGKLNLSKEGR MVVLSLVLGLSEQDDFANIP DLQNPGTQQNQNAQGDKR in isoform 7. 1
Alternative sequenceiVSP_058245123 – 403Missing in isoform 8. Add BLAST281

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20106 mRNA Translation: AAA87725.1
AF336852 mRNA Translation: AAK01447.1
AF336853 mRNA Translation: AAK01448.1
AF336854 mRNA Translation: AAK01449.1
AF336855 mRNA Translation: AAK01450.1
AF336856 mRNA Translation: AAK01451.1
AF336857 mRNA Translation: AAK01452.1
AF336858 mRNA Translation: AAK01453.1
AF336859 mRNA Translation: AAK01454.1
AF336860 mRNA Translation: AAK01455.1
AC095662 Genomic DNA No translation available.
AC130565 Genomic DNA No translation available.
CH473953 Genomic DNA Translation: EDM12801.1
CH473953 Genomic DNA Translation: EDM12802.1
CH473953 Genomic DNA Translation: EDM12803.1
CH473953 Genomic DNA Translation: EDM12804.1
CH473953 Genomic DNA Translation: EDM12805.1
CH473953 Genomic DNA Translation: EDM12806.1
CH473953 Genomic DNA Translation: EDM12807.1
PIRiS58400
RefSeqiNP_067691.1, NM_021659.1 [Q62747-1]
XP_006231127.1, XM_006231065.3 [Q62747-2]
XP_006231129.1, XM_006231067.3 [Q62747-4]
XP_006231130.1, XM_006231068.3 [Q62747-3]
UniGeneiRn.91884

Genome annotation databases

EnsembliENSRNOT00000007429; ENSRNOP00000007429; ENSRNOG00000026432 [Q62747-8]
ENSRNOT00000035576; ENSRNOP00000031890; ENSRNOG00000026432 [Q62747-4]
ENSRNOT00000036168; ENSRNOP00000031944; ENSRNOG00000026432 [Q62747-6]
ENSRNOT00000036180; ENSRNOP00000032388; ENSRNOG00000026432 [Q62747-3]
ENSRNOT00000036232; ENSRNOP00000031195; ENSRNOG00000026432 [Q62747-2]
ENSRNOT00000036283; ENSRNOP00000031816; ENSRNOG00000026432 [Q62747-7]
ENSRNOT00000047964; ENSRNOP00000049360; ENSRNOG00000026432 [Q62747-5]
ENSRNOT00000048704; ENSRNOP00000040667; ENSRNOG00000026432 [Q62747-1]
GeneIDi59267
KEGGirno:59267
UCSCiRGD:62013 rat

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSYT7_RAT
AccessioniPrimary (citable) accession number: Q62747
Secondary accession number(s): F1M262
, Q99J98, Q99P33, Q99P34, Q99P35, Q99P36, Q99P37, Q99P38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2016
Last sequence update: November 1, 1996
Last modified: September 12, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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