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Protein

Lamina-associated polypeptide 2, isoform beta

Gene

Tmpo

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds directly to lamin B1 and chromosomes in a mitotic phosphorylation-regulated manner. May play an important role in nuclear envelope reassembly at the end of mitosis and/or anchoring of the nuclear lamina and interphase chromosomes to the nuclear envelope.

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • lamin binding Source: RGD

GO - Biological processi

  • nuclear envelope reassembly Source: RGD

Keywordsi

Molecular functionDNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-4419969 Depolymerisation of the Nuclear Lamina

Names & Taxonomyi

Protein namesi
Recommended name:
Lamina-associated polypeptide 2, isoform beta
Alternative name(s):
Thymopoietin isoform beta
Short name:
TP beta
Gene namesi
Name:Tmpo
Synonyms:Lap2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi3875 Tmpo

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei410 – 430Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini431 – 452LumenalSequence analysisAdd BLAST22

Keywords - Cellular componenti

Chromosome, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002061471 – 452Lamina-associated polypeptide 2, isoform betaAdd BLAST452

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei66PhosphoserineCombined sources1
Modified residuei67PhosphoserineCombined sources1
Modified residuei74PhosphothreonineCombined sources1
Modified residuei82PhosphoserineBy similarity1
Modified residuei85Omega-N-methylarginineBy similarity1
Modified residuei87Omega-N-methylarginineBy similarity1
Modified residuei155PhosphoserineCombined sources1
Modified residuei158PhosphoserineBy similarity1
Modified residuei159PhosphothreonineCombined sources1
Modified residuei165PhosphoserineBy similarity1
Modified residuei167PhosphoserineCombined sources1
Modified residuei176PhosphoserineBy similarity1
Modified residuei179PhosphoserineBy similarity1
Modified residuei183PhosphoserineCombined sources1
Modified residuei206N6-acetyllysineBy similarity1
Modified residuei305PhosphoserineBy similarity1
Modified residuei306PhosphoserineBy similarity1
Modified residuei361PhosphoserineBy similarity1
Modified residuei388N6-acetyllysineBy similarity1

Post-translational modificationi

Mitosis-specific phosphorylation specifically abolishes its binding to lamin B and chromosomes.

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ62733
PRIDEiQ62733

PTM databases

iPTMnetiQ62733
PhosphoSitePlusiQ62733

Expressioni

Gene expression databases

BgeeiENSRNOG00000008797 Expressed in 10 organ(s), highest expression level in colon
GenevisibleiQ62733 RN

Interactioni

Subunit structurei

Interacts with LMNB1, LMNB2, BANF1, AKAP8L, GMCL and chromosomes.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi247398, 1 interactor
STRINGi10116.ENSRNOP00000012715

Structurei

3D structure databases

ProteinModelPortaliQ62733
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 48LEM-likePROSITE-ProRule annotationAdd BLAST44
Domaini108 – 152LEMPROSITE-ProRule annotationAdd BLAST45

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 409NucleoplasmicSequence analysisAdd BLAST409
Regioni49 – 107LinkerAdd BLAST59
Regioni137 – 242NAKAP95-binding NAdd BLAST106
Regioni298 – 370Binds lamins BAdd BLAST73
Regioni299 – 373NAKAP95-binding CAdd BLAST75

Domaini

Has two structurally independent, non-interacting domains: LEM-like (also called LAP2-N or LEM-D) and LEM (also called LAP2-C or LEM-B). LEM-like binds DNA while LEM interacts with BANF1 (By similarity).By similarity

Sequence similaritiesi

Belongs to the LEM family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IT7C Eukaryota
ENOG4111J0N LUCA
GeneTreeiENSGT00510000048934
HOGENOMiHOG000113280
HOVERGENiHBG000166
InParanoidiQ62733
OMAiTNQGNPF
OrthoDBiEOG091G03A8
PhylomeDBiQ62733

Family and domain databases

Gene3Di1.10.720.40, 2 hits
InterProiView protein in InterPro
IPR013146 LEM-like_dom
IPR011015 LEM/LEM-like_dom_sf
IPR003887 LEM_dom
PfamiView protein in Pfam
PF03020 LEM, 1 hit
PF08198 Thymopoietin, 1 hit
SMARTiView protein in SMART
SM00540 LEM, 1 hit
SM01261 Thymopoietin, 1 hit
SUPFAMiSSF63451 SSF63451, 2 hits
PROSITEiView protein in PROSITE
PS50954 LEM, 1 hit
PS50955 LEM_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

Q62733-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPEFLEDPSV LTKDKLKSEL VANNVTLPAG EQRKDVYVQL YLQHLTARNR
60 70 80 90 100
PPLAAGANSK GPPDFSSDEE REPTPVLGSG ASVGRGRGAV GRKATKKTDK
110 120 130 140 150
PRPEDKDDLD VTELSNEELL EQLVRYGVNP GPIVGTTRKL YEKKLLKLRE
160 170 180 190 200
QGAESRSSTP LPTVSSSAEN TRQNGSNDSD RYSDNDEDSK IELKLEKREP
210 220 230 240 250
LKGRAKTPVT LKQRRIEHNQ SYSEAGVTET EWTSGSSKGG PLQALTREST
260 270 280 290 300
RGSRRTPRRR VEPSQHFRVD GAVISESTPI AETIKASSND SLVANRLTGN
310 320 330 340 350
FKHASSILPI TEFSDITRRT PKKPLTRAEV GEKTEERRVE RDILKEMFPY
360 370 380 390 400
EASTPTGISA SCRRPIKGAA GRPLELSDFR MEESFSSKYV PKYVPLADVK
410 420 430 440 450
SEKTKKGRSV PMWIKMLLFA LVAGFLFLVY QAMETNQGNP FTNFLQDTKI

SN
Length:452
Mass (Da):50,278
Last modified:January 23, 2007 - v3
Checksum:iD56C9B51DB9D3DA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18314 mRNA Translation: AAC52209.1
PIRiS55255
RefSeqiNP_037019.1, NM_012887.1
UniGeneiRn.202955

Genome annotation databases

EnsembliENSRNOT00000012715; ENSRNOP00000012715; ENSRNOG00000008797
GeneIDi25359
KEGGirno:25359
UCSCiRGD:3875 rat

Similar proteinsi

Entry informationi

Entry nameiLAP2_RAT
AccessioniPrimary (citable) accession number: Q62733
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 126 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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