UniProtKB - Q62724 (MCM6_RAT)
Protein
DNA replication licensing factor MCM6
Gene
Mcm6
Organism
Rattus norvegicus (Rat)
Status
Functioni
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.By similarity
Catalytic activityi
- EC:3.6.4.12
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 82 – 89 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA helicase activity Source: UniProtKB-EC
- DNA replication origin binding Source: GO_Central
- identical protein binding Source: RGD
- single-stranded DNA binding Source: RGD
GO - Biological processi
- DNA unwinding involved in DNA replication Source: RGD
- double-strand break repair via break-induced replication Source: GO_Central
- mitotic DNA replication Source: GO_Central
- pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | Cell cycle, DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: DNA replication licensing factor MCM6 (EC:3.6.4.12)Alternative name(s): Intestinal DNA replication protein |
Gene namesi | Name:Mcm6 Synonyms:Mcmd6 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61967, Mcm6 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Chromosome By similarity
Note: Binds to chromatin during G1 and detach from it during S phase.By similarity
Nucleus
- CMG complex Source: UniProtKB
- nucleus Source: RGD
Other locations
- MCM complex Source: RGD
Keywords - Cellular componenti
Chromosome, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194115 | ‹1 – 507 | DNA replication licensing factor MCM6Add BLAST | ›507 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 329 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 375 | PhosphoserineBy similarity | 1 | |
Modified residuei | 390 | PhosphoserineBy similarity | 1 | |
Modified residuei | 448 | PhosphoserineCombined sources | 1 | |
Modified residuei | 477 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity
Keywords - PTMi
Acetylation, Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | Q62724 |
PaxDbi | Q62724 |
PeptideAtlasi | Q62724 |
PTM databases
iPTMneti | Q62724 |
Interactioni
Subunit structurei
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. May interact with MCM10.
Interacts with TIPIN.
Interacts with CDT1.
Interacts with MCMBP.
Interacts with DDI2 (By similarity).
Component of the CMG helicase complex, composed of the MCM2-7 complex, the GINS complex and CDC45 (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000004969 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 239 | MCMAdd BLAST | 208 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 214 – 217 | Arginine finger | 4 |
Sequence similaritiesi
Belongs to the MCM family.Curated
Phylogenomic databases
eggNOGi | KOG0480, Eukaryota |
InParanoidi | Q62724 |
PhylomeDBi | Q62724 |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR041024, Mcm6_C IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF18263, MCM6_C, 1 hit PF17855, MCM_lid, 1 hit |
PRINTSi | PR01657, MCMFAMILY |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
(1+)i Sequence
Sequence statusi: Fragment.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q62724-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
LRDEEQTAES IKNQMTVKEW EKVFEMSQDQ NLYHNLCTSL FPTIHGNDEV
60 70 80 90 100
KRGVLLMLFG GVPKTTGEGT SLRGDINVCI VGDPSTAKSQ FLKHVDEFSP
110 120 130 140 150
RAVYTSGKAS SASGLTAAVV RDEESHEFVI EAGALMLADN GVCCIDEFDK
160 170 180 190 200
MDMRDQVAIH EAMEQQTISI TKAGVKATLN ARTSILAAAN PVSGHYDRSK
210 220 230 240 250
SLKQNINLSA PIMSRFDLFF ILVDECNEVT DYAIARRIVD LHSRIEESID
260 270 280 290 300
RVYSLDDIRR YLLFARQFKP KISKESEDFI VEQYKRLRQR DGSGITKSSW
310 320 330 340 350
RITVRQLESM IRLSESMARM HCCDEVQPKH VKEAFRLLNK SIIRVETPDV
360 370 380 390 400
NLDQEEEIQM ETDEGPGGIN GHADSPAPVN GFNGSGEDAS QETVPKPSLR
410 420 430 440 450
LAFAEYCRIS NLIVLHLRKM EEEEDESALK RSELVNWYLK EIESEIDSEE
460 470 480 490 500
ELINKKRIIE KVVHRLTHYD HVLIELTQAG LKGSSEGSES YEEDPYLVVN
PNYLLED
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA9CMB8 | A9CMB8_RAT | DNA helicase | Mcm6 rCG_45934 | 821 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U17565 mRNA Translation: AAC18424.1 |
PIRi | T10753 |
Genome annotation databases
UCSCi | RGD:61967, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U17565 mRNA Translation: AAC18424.1 |
PIRi | T10753 |
3D structure databases
SMRi | Q62724 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000004969 |
PTM databases
iPTMneti | Q62724 |
Proteomic databases
jPOSTi | Q62724 |
PaxDbi | Q62724 |
PeptideAtlasi | Q62724 |
Genome annotation databases
UCSCi | RGD:61967, rat |
Organism-specific databases
RGDi | 61967, Mcm6 |
Phylogenomic databases
eggNOGi | KOG0480, Eukaryota |
InParanoidi | Q62724 |
PhylomeDBi | Q62724 |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR041024, Mcm6_C IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF18263, MCM6_C, 1 hit PF17855, MCM_lid, 1 hit |
PRINTSi | PR01657, MCMFAMILY |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCM6_RAT | |
Accessioni | Q62724Primary (citable) accession number: Q62724 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | December 15, 1998 | |
Last modified: | December 2, 2020 | |
This is version 121 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families