UniProtKB - Q62673 (PLK1_RAT)
Serine/threonine-protein kinase PLK1
Plk1
Functioni
Catalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 82 | ATPPROSITE-ProRule annotation | 1 | |
Binding sitei | 131 | ATP; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Active sitei | 176 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Binding sitei | 194 | ATPPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 59 – 67 | ATPPROSITE-ProRule annotation | 9 | |
Nucleotide bindingi | 178 – 181 | ATPPROSITE-ProRule annotation | 4 |
GO - Molecular functioni
- anaphase-promoting complex binding Source: RGD
- ATP binding Source: RGD
- identical protein binding Source: RGD
- magnesium ion binding Source: RGD
- microtubule binding Source: UniProtKB
- protein kinase activity Source: RGD
- protein kinase binding Source: RGD
- protein serine/threonine kinase activity Source: UniProtKB
GO - Biological processi
- centrosome cycle Source: UniProtKB
- establishment of mitotic spindle orientation Source: RGD
- establishment of protein localization Source: RGD
- female meiosis chromosome segregation Source: RGD
- G2/M transition of mitotic cell cycle Source: UniProtKB
- homologous chromosome segregation Source: RGD
- microtubule bundle formation Source: UniProtKB
- mitotic cell cycle Source: UniProtKB
- mitotic cytokinesis Source: UniProtKB
- mitotic sister chromatid segregation Source: UniProtKB
- mitotic spindle assembly checkpoint Source: UniProtKB
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: RGD
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- nuclear envelope disassembly Source: UniProtKB
- peptidyl-serine phosphorylation Source: RGD
- polar body extrusion after meiotic divisions Source: RGD
- positive regulation of peptidyl-threonine phosphorylation Source: RGD
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
- positive regulation of proteolysis Source: RGD
- positive regulation of ubiquitin protein ligase activity Source: UniProtKB
- positive regulation of ubiquitin-protein transferase activity Source: UniProtKB
- protein destabilization Source: RGD
- protein localization to chromatin Source: UniProtKB
- protein localization to nuclear envelope Source: UniProtKB
- protein localization to organelle Source: RGD
- protein phosphorylation Source: MGI
- protein ubiquitination Source: RGD
- regulation of cytokinesis Source: RGD
- regulation of mitotic cell cycle Source: RGD
- regulation of mitotic metaphase/anaphase transition Source: RGD
- regulation of mitotic spindle assembly Source: RGD
- regulation of protein binding Source: RGD
- regulation of protein localization to cell cortex Source: RGD
- response to antibiotic Source: RGD
- signal transduction involved in G2 DNA damage checkpoint Source: UniProtKB
- synaptonemal complex disassembly Source: RGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cell cycle, Cell division, Mitosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.21, 5301 |
Reactomei | R-RNO-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-RNO-156711, Polo-like kinase mediated events R-RNO-162658, Golgi Cisternae Pericentriolar Stack Reorganization R-RNO-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-RNO-176412, Phosphorylation of the APC/C R-RNO-176417, Phosphorylation of Emi1 R-RNO-2299718, Condensation of Prophase Chromosomes R-RNO-2467813, Separation of Sister Chromatids R-RNO-2500257, Resolution of Sister Chromatid Cohesion R-RNO-2565942, Regulation of PLK1 Activity at G2/M Transition R-RNO-2980767, Activation of NIMA Kinases NEK9, NEK6, NEK7 R-RNO-380259, Loss of Nlp from mitotic centrosomes R-RNO-380270, Recruitment of mitotic centrosome proteins and complexes R-RNO-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-RNO-380320, Recruitment of NuMA to mitotic centrosomes R-RNO-5620912, Anchoring of the basal body to the plasma membrane R-RNO-5663220, RHO GTPases Activate Formins R-RNO-68877, Mitotic Prometaphase R-RNO-68881, Mitotic Metaphase/Anaphase Transition R-RNO-68884, Mitotic Telophase/Cytokinesis R-RNO-69273, Cyclin A/B1/B2 associated events during G2/M transition R-RNO-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-RNO-8854518, AURKA Activation by TPX2 R-RNO-9648025, EML4 and NUDC in mitotic spindle formation |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein kinase PLK1 (EC:2.7.11.21)Alternative name(s): Polo-like kinase 1 Short name: PLK-1 |
Gene namesi | Name:Plk1 Synonyms:Plk |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3352, Plk1 |
Subcellular locationi
Cytoskeleton
- centrosome By similarity
- spindle By similarity
Nucleus
- Nucleus By similarity
Other locations
- kinetochore By similarity
- Midbody By similarity
Note: localization at the centrosome starts at the G1/S transition (By similarity). During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGO1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization. Localizes onto the central spindle by phosphorylating and docking at midzone proteins KIF20A/MKLP2 and PRC1 (By similarity). Colocalizes with FRY to separating centrosomes and spindle poles from prophase to metaphase in mitosis, but not in other stages of the cell cycle (By similarity). Localization to the centrosome is required for S phase progression (By similarity). Colocalizes with HSF1 at the spindle poles during prometaphase (By similarity).By similarity
Cytoskeleton
- centriolar satellite Source: RGD
- centriole Source: RGD
- centrosome Source: UniProtKB
- microtubule cytoskeleton Source: RGD
- mitotic spindle pole Source: RGD
- spindle Source: UniProtKB
- spindle midzone Source: RGD
- spindle pole Source: RGD
Nucleus
- condensed nuclear chromosome outer kinetochore Source: RGD
- condensed nuclear chromosome, centromeric region Source: RGD
- nucleus Source: RGD
- synaptonemal complex Source: RGD
Other locations
- chromatin Source: RGD
- chromosome, centromeric region Source: RGD
- cytoplasm Source: RGD
- kinetochore Source: UniProtKB
- midbody Source: UniProtKB
Keywords - Cellular componenti
Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086558 | 1 – 603 | Serine/threonine-protein kinase PLK1Add BLAST | 603 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 19 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 103 | PhosphoserineBy similarity | 1 | |
Modified residuei | 137 | PhosphoserineBy similarity | 1 | |
Modified residuei | 210 | Phosphothreonine; by AURKABy similarity | 1 | |
Modified residuei | 214 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 269 | Phosphoserine; by autocatalysisBy similarity | 1 | |
Modified residuei | 335 | Phosphoserine; by autocatalysisBy similarity | 1 | |
Cross-linki | 338 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 375 | PhosphoserineBy similarity | 1 | |
Modified residuei | 450 | PhosphoserineBy similarity | 1 | |
Cross-linki | 492 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 498 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q62673 |
PaxDbi | Q62673 |
PTM databases
iPTMneti | Q62673 |
PhosphoSitePlusi | Q62673 |
Interactioni
Subunit structurei
Interacts with CEP170 and EVI5.
Interacts and phosphorylates ERCC6L. Interacts with FAM29A.
Interacts with SLX4/BTBD12 and TTDN1.
Interacts with BUB1B.
Interacts (via POLO-box domain) with the phosphorylated form of BUB1, CENPU and CDC25C.
Interacts with isoform 3 of SGO1.
Interacts with BORA, KIF2A and AURKA.
Interacts with TOPORS and CYLD.
Interacts with ECT2; the interaction is stimulated upon phosphorylation of ECT2 on 'Thr-444'.
Interacts with PRC1.
Interacts with KIF20A/MKLP2 (when phosphorylated), leading to the recruitment at the central spindle.
Interacts (via POLO box domains) with PPP1R12A/MYPT1 (when previously phosphorylated by CDK1).
Part of an astrin (SPAG5)-kinastrin (SKAP) complex containing KNSTRN, SPAG5, PLK1, DYNLL1 and SGO2.
Interacts with BIRC6/bruce.
Interacts with CDK1-phosphorylated FRY; this interaction occurs in mitotic cells, but not in interphase cells. FRY interaction facilitates AURKA-mediated PLK1 phosphorylation.
Interacts with CDK1-phosphorylated DCTN6 during mitotic prometaphase; the interaction facilitates recruitment to kinetochores.
Interacts with CEP68; the interaction phosphorylates CEP68.
Interacts (via POLO-box domain) with DCTN1.
Interacts with CEP20 in later G1, S, G2 and M phases of the cell cycle; this interaction recruits PLK1 to centrosomes, a step required for S phase progression.
Interacts with HSF1; this interaction increases upon heat shock but does not modulate neither HSF1 homotrimerization nor DNA-binding activities.
Interacts with HNRNPU; this interaction induces phosphorylation of HNRNPU in mitosis.
Interacts (via its N-terminus) with RIOK2 (By similarity).
Interacts with KLHL22 (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
- microtubule binding Source: UniProtKB
- protein kinase binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000025629 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 53 – 305 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 253 | |
Domaini | 417 – 480 | POLO box 1PROSITE-ProRule annotationAdd BLAST | 64 | |
Domaini | 515 – 584 | POLO box 2PROSITE-ProRule annotationAdd BLAST | 70 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 194 – 221 | Activation loopBy similarityAdd BLAST | 28 | |
Regioni | 493 – 507 | LinkerBy similarityAdd BLAST | 15 | |
Regioni | 538 – 540 | Important for interaction with phosphorylated proteinsBy similarity | 3 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 337 – 340 | D-box that targets the protein for proteasomal degradation in anaphaseBy similarity | 4 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0575, Eukaryota |
InParanoidi | Q62673 |
OrthoDBi | 507604at2759 |
Family and domain databases
CDDi | cd13118, POLO_box_1, 1 hit cd13117, POLO_box_2, 1 hit cd14187, STKc_PLK1, 1 hit |
Gene3Di | 3.30.1120.30, 2 hits |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR033702, PLK1_cat IPR033701, POLO_box_1 IPR033695, POLO_box_2 IPR000959, POLO_box_dom IPR036947, POLO_box_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF00659, POLO_box, 2 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50078, POLO_BOX, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MNAAAKAGKL ARAPADLGKG GVPGDAAPGA PGAAPLAKEI PEVLMDPRSR
60 70 80 90 100
RQYVRGRFLG KGGFAKCFEI SDSDTKEVFP GKIVPKSLLL KPHQKEKMSM
110 120 130 140 150
EISIHRSLAH QHVVGFHGFF EDSDFVFVVL ELCRRRSLLE LHKRRKALTE
160 170 180 190 200
PEARYYLRQI VLGCQYLHRN QVIHRDLKLG NLFLNEDLEV KIGDFGLATK
210 220 230 240 250
VEYEGERKKT LCGTPNYIAP EVLSKKGHSF EVDVWSIGCI MYTLLVGKPP
260 270 280 290 300
FETSCLKETY LRIKKNEYSI PKHINPVAAS LIQKMLQTDP TARPTIHELL
310 320 330 340 350
NDEFFTSGYI PARLPITCLT IPPRFSIAPS SLDPSNRKPL TVLNKGVENP
360 370 380 390 400
LPDRPREKEE PVVRETNEAI ECHLSDLLQQ LTSVNASKPS ERGLVRQEEA
410 420 430 440 450
EDPACIPIFW VSKWVDYSDK YGLGYQLCDN SVGVLFNDST RLILYNDGDS
460 470 480 490 500
LQYIERDGTE SYLTVSSHPN SLMKKITLLN YFRNYMSEHL LKAGANITPR
510 520 530 540 550
EGDELARLPY LRTWFRTRSA IILHLSNGTV QINFFQDHTK LILCPLMAAV
560 570 580 590 600
TYINEKRDFR TYRLSLLEEY GCCKELASRL RYARTMVDKL LSSRSACNRL
KAS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A140TAD2 | A0A140TAD2_RAT | Serine/threonine-protein kinase PLK | Plk1 | 603 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 27 | A → V in AAA18885 (Ref. 1) Curated | 1 | |
Sequence conflicti | 32 | G → V in AAA18885 (Ref. 1) Curated | 1 | |
Sequence conflicti | 45 | M → V in AAA18885 (Ref. 1) Curated | 1 | |
Sequence conflicti | 51 | R → Q in AAA18885 (Ref. 1) Curated | 1 | |
Sequence conflicti | 80 | P → A in AAH83926 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 102 | I → T in AAA18885 (Ref. 1) Curated | 1 | |
Sequence conflicti | 109 | A → E in AAA18885 (Ref. 1) Curated | 1 | |
Sequence conflicti | 291 | T → A in AAA18885 (Ref. 1) Curated | 1 | |
Sequence conflicti | 543 – 544 | LC → RG in AAA18885 (Ref. 1) Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U10188 mRNA Translation: AAA18885.1 BC083926 mRNA Translation: AAH83926.1 |
RefSeqi | NP_058796.1, NM_017100.1 |
Genome annotation databases
GeneIDi | 25515 |
KEGGi | rno:25515 |
UCSCi | RGD:3352, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U10188 mRNA Translation: AAA18885.1 BC083926 mRNA Translation: AAH83926.1 |
RefSeqi | NP_058796.1, NM_017100.1 |
3D structure databases
SMRi | Q62673 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000025629 |
PTM databases
iPTMneti | Q62673 |
PhosphoSitePlusi | Q62673 |
Proteomic databases
jPOSTi | Q62673 |
PaxDbi | Q62673 |
Genome annotation databases
GeneIDi | 25515 |
KEGGi | rno:25515 |
UCSCi | RGD:3352, rat |
Organism-specific databases
CTDi | 5347 |
RGDi | 3352, Plk1 |
Phylogenomic databases
eggNOGi | KOG0575, Eukaryota |
InParanoidi | Q62673 |
OrthoDBi | 507604at2759 |
Enzyme and pathway databases
BRENDAi | 2.7.11.21, 5301 |
Reactomei | R-RNO-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-RNO-156711, Polo-like kinase mediated events R-RNO-162658, Golgi Cisternae Pericentriolar Stack Reorganization R-RNO-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-RNO-176412, Phosphorylation of the APC/C R-RNO-176417, Phosphorylation of Emi1 R-RNO-2299718, Condensation of Prophase Chromosomes R-RNO-2467813, Separation of Sister Chromatids R-RNO-2500257, Resolution of Sister Chromatid Cohesion R-RNO-2565942, Regulation of PLK1 Activity at G2/M Transition R-RNO-2980767, Activation of NIMA Kinases NEK9, NEK6, NEK7 R-RNO-380259, Loss of Nlp from mitotic centrosomes R-RNO-380270, Recruitment of mitotic centrosome proteins and complexes R-RNO-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-RNO-380320, Recruitment of NuMA to mitotic centrosomes R-RNO-5620912, Anchoring of the basal body to the plasma membrane R-RNO-5663220, RHO GTPases Activate Formins R-RNO-68877, Mitotic Prometaphase R-RNO-68881, Mitotic Metaphase/Anaphase Transition R-RNO-68884, Mitotic Telophase/Cytokinesis R-RNO-69273, Cyclin A/B1/B2 associated events during G2/M transition R-RNO-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-RNO-8854518, AURKA Activation by TPX2 R-RNO-9648025, EML4 and NUDC in mitotic spindle formation |
Miscellaneous databases
PROi | PR:Q62673 |
Family and domain databases
CDDi | cd13118, POLO_box_1, 1 hit cd13117, POLO_box_2, 1 hit cd14187, STKc_PLK1, 1 hit |
Gene3Di | 3.30.1120.30, 2 hits |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR033702, PLK1_cat IPR033701, POLO_box_1 IPR033695, POLO_box_2 IPR000959, POLO_box_dom IPR036947, POLO_box_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF00659, POLO_box, 2 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50078, POLO_BOX, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PLK1_RAT | |
Accessioni | Q62673Primary (citable) accession number: Q62673 Secondary accession number(s): F1LNH6, Q5XHX4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | December 14, 2011 | |
Last modified: | December 2, 2020 | |
This is version 172 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families