UniProtKB - Q62671 (UBR5_RAT)
Protein
E3 ubiquitin-protein ligase UBR5
Gene
Ubr5
Organism
Rattus norvegicus (Rat)
Status
Functioni
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) for the DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Also acts as a regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes (By similarity).By similarity
Miscellaneous
A cysteine residue is required for ubiquitin-thioester formation.
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.26
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 2757 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1166 – 1234 | UBR-typePROSITE-ProRule annotationAdd BLAST | 69 |
GO - Molecular functioni
- protein domain specific binding Source: RGD
- RNA binding Source: InterPro
- ubiquitin binding Source: InterPro
- ubiquitin protein ligase activity Source: RGD
- ubiquitin-ubiquitin ligase activity Source: RGD
- zinc ion binding Source: InterPro
GO - Biological processi
- cellular response to DNA damage stimulus Source: UniProtKB
- DNA repair Source: UniProtKB-KW
- negative regulation of double-strand break repair Source: UniProtKB
- negative regulation of histone H2A K63-linked ubiquitination Source: UniProtKB
- negative regulation of interleukin-17 production Source: RGD
- positive regulation of canonical Wnt signaling pathway Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of protein import into nucleus Source: RGD
- progesterone receptor signaling pathway Source: RGD
- protein K48-linked ubiquitination Source: RGD
- protein polyubiquitination Source: RGD
- protein ubiquitination Source: RGD
- regulation of double-strand break repair Source: GO_Central
- regulation of protein stability Source: RGD
Keywordsi
Molecular function | Transferase |
Biological process | DNA damage, DNA repair, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase UBR5 (EC:2.3.2.26)Alternative name(s): 100 kDa protein E3 ubiquitin-protein ligase, HECT domain-containing 1 HECT-type E3 ubiquitin transferase UBR5 Hyperplastic discs protein homolog |
Gene namesi | Name:Ubr5 Synonyms:Dd5, Edd, Edd1, Hyd |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621236, Ubr5 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytosol
- cytosol Source: RGD
Nucleus
- nucleus Source: RGD
Other locations
- perinuclear region of cytoplasm Source: RGD
- protein-containing complex Source: RGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086933 | 1 – 2788 | E3 ubiquitin-protein ligase UBR5Add BLAST | 2788 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 100 | PhosphoserineBy similarity | 1 | |
Modified residuei | 317 | PhosphoserineBy similarity | 1 | |
Modified residuei | 342 | PhosphoserineBy similarity | 1 | |
Modified residuei | 567 | PhosphoserineBy similarity | 1 | |
Modified residuei | 601 | PhosphoserineBy similarity | 1 | |
Modified residuei | 626 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 797 | PhosphoserineBy similarity | 1 | |
Modified residuei | 917 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1007 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1104 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1124 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1216 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1297 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1344 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1364 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1470 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1538 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1725 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1730 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1735 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 1769 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1959 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1980 | PhosphoserineBy similarity | 1 | |
Modified residuei | 2016 | PhosphoserineBy similarity | 1 | |
Modified residuei | 2018 | PhosphoserineBy similarity | 1 | |
Modified residuei | 2020 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 2066 | PhosphoserineBy similarity | 1 | |
Modified residuei | 2203 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 2231 | PhosphoserineCombined sources | 1 | |
Modified residuei | 2279 | PhosphoserineBy similarity | 1 | |
Modified residuei | 2459 | PhosphoserineBy similarity | 1 | |
Modified residuei | 2473 | PhosphoserineBy similarity | 1 | |
Modified residuei | 2475 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q62671 |
PaxDbi | Q62671 |
PRIDEi | Q62671 |
PTM databases
iPTMneti | Q62671 |
PhosphoSitePlusi | Q62671 |
Expressioni
Tissue specificityi
Highest levels found in testis. Also present in liver, kidney, lung and brain.1 Publication
Developmental stagei
In early postnatal life, expression in the testis increases to reach a maximum around day 28.1 Publication
Interactioni
Subunit structurei
Binds TOPBP1. Associates with CDK9 and TFIIS/TCEA1 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulate target gene transcription by recruiting their promoters.
Interacts with PIH1D1.
By similarityGO - Molecular functioni
- protein domain specific binding Source: RGD
- ubiquitin binding Source: InterPro
Protein-protein interaction databases
ELMi | Q62671 |
IntActi | Q62671, 1 interactor |
STRINGi | 10116.ENSRNOP00000009115 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q62671 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2367 – 2444 | PABCPROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 2451 – 2788 | HECTPROSITE-ProRule annotationAdd BLAST | 338 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1613 – 1670 | Ser-richAdd BLAST | 58 | |
Compositional biasi | 2319 – 2366 | Arg-richAdd BLAST | 48 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1166 – 1234 | UBR-typePROSITE-ProRule annotationAdd BLAST | 69 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0943, Eukaryota |
InParanoidi | Q62671 |
Family and domain databases
CDDi | cd14423, CUE_UBR5, 1 hit |
InterProi | View protein in InterPro IPR024725, E3_UbLigase_EDD_UBA IPR000569, HECT_dom IPR035983, Hect_E3_ubiquitin_ligase IPR036053, PABP-dom IPR002004, PABP_HYD IPR009091, RCC1/BLIP-II IPR003126, Znf_UBR |
Pfami | View protein in Pfam PF11547, E3_UbLigase_EDD, 1 hit PF00632, HECT, 1 hit PF00658, PABP, 1 hit |
SMARTi | View protein in SMART SM00119, HECTc, 1 hit SM00517, PolyA, 1 hit SM00396, ZnF_UBR1, 1 hit |
SUPFAMi | SSF50985, SSF50985, 1 hit SSF56204, SSF56204, 1 hit SSF63570, SSF63570, 1 hit |
PROSITEi | View protein in PROSITE PS50237, HECT, 1 hit PS51309, PABC, 1 hit PS51157, ZF_UBR, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q62671-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNKQAVKRLH MLREVSEKLN KYNLNSHPPL NVLEQATIKQ CVVGPNHAAF
60 70 80 90 100
LLEDGRICRI GFSVQPDRLE LGKPDNNDGS KLNSSSGTGR TSRPGRTSDS
110 120 130 140 150
PWFLSGSETL GRLAGNTLGS RWSSGVGGSG GGSSGRSSAG ARDSRRQTRV
160 170 180 190 200
IRTGRDRGSG LLGSQPQPVI PASVIPEELI SQAQVVLQGK SRSVIIRELQ
210 220 230 240 250
RTNLDVNLAV NNLLSRDDED GDDGDDTASE SYLPGEDLMS LLDADIHSAH
260 270 280 290 300
PSVIIDADAM FSEDISYFGY PSFRRSSLSR LGSSRVLLLP LERDSELLRE
310 320 330 340 350
RESVLRLRER RWLDGASFDN ERGSTSKEGE PNPDKKNTPV QSPVSLGEDL
360 370 380 390 400
QWWPDKDGTK FTCIGALYSE LVAVSSKGEL YQWKWTESEP YRNAQNPSLH
410 420 430 440 450
HPRATFLGLT NEKIVLLSAN SIRATVATEN NKVATWVDET LSSVASKLEH
460 470 480 490 500
TAQTYSELQG ERIVSLHCCA LYTCAQLENN LYWWGVVPFS QRKKMLEKAR
510 520 530 540 550
AKNKKPKSSA GVQSLNVRGG RQVCLRNNPL YHAGAVAFSI SAGIPKVGVL
560 570 580 590 600
MESVWNMNDS CRFQLRSPES LKSMEKASKT IETKPESKQE PVKTEMGPPP
610 620 630 640 650
SPASTCSDAS SIASSASMPY KRRRSTPAPK EEEKVNEEQW SLREVVFVED
660 670 680 690 700
VKNVPVGKVL KVDGAYVAVK FPGTSSNTNC QNSSGPDADP SSLLQDCRLL
710 720 730 740 750
RIDELQVVKT GGTPKVPDCF QRTPKKLCIP EKTEILAVNV DSKGVHAVLK
760 770 780 790 800
TGNWVRYCIF DLATGKAEQE NNFPTSSVAF LGQNERSVAI FTAGQESPII
810 820 830 840 850
LRDGNGTIYP MAKDCMGGIR DPDWLDLPPI SSLGMGVHSL INLPANSTIK
860 870 880 890 900
KKAAIIIMAV EKQTLMQHIL RCDYEACRQY LVNLEQAVVL EQNLQMLQTF
910 920 930 940 950
ISHRCDGNRN ILHACVSVCF PTSNKETKEE EEAERSERNT FAERLSAVEA
960 970 980 990 1000
IANAISVVSS NGPGNRAGSS SSRSLRLREM MRRSLRAAGL GRHEAGASSS
1010 1020 1030 1040 1050
DHQDPVSPPI APPSWVPDPP SMDPDGDIDF ILAPAVGSLT TAATGGGQGP
1060 1070 1080 1090 1100
STSTIPGPST EPSVVESKDR KANAHFILKL LCDSAVLQPY LRELLSAKDA
1110 1120 1130 1140 1150
RGMTPFMSAV SGRAYPAAIT ILETAQKIAK AEVSGSEKEE DVFMGMVCPS
1160 1170 1180 1190 1200
GTNPDDSPLY VLCCNDTCSF TWTGAEHINQ DIFECRTCGL LESLCCCTEC
1210 1220 1230 1240 1250
ARVCHKGHDC KLKRTSPTAY CDCWEKCKCK TLIAGQKSAR LDLLYRLLTA
1260 1270 1280 1290 1300
TNLVTLPNSR GEHLLLFLVQ TVARQTVEHC QYRPPRIRED RNRKTASPDD
1310 1320 1330 1340 1350
SDMPDHDLEP PRFAQLALER VLQDWNALRS MIMFGSQENK DPLSASSRIG
1360 1370 1380 1390 1400
HLLPEEQVYL NQQSGTIRLD CFTHCLIVKC TADILLLDTL LGTLVKELQN
1410 1420 1430 1440 1450
KYTPGRREEA IAVTMRFLRS VARVFVILSV EMASSKKKNN FIPQPIGKCK
1460 1470 1480 1490 1500
RVFQALLPYA VEELCNVAES LIVPVRMGIA RPTAPFTLAS TSIDAMQGSE
1510 1520 1530 1540 1550
ELFSVEPLPP RPSSDQSSSS SQSQSSYIIR NPQQRRISQS QPVRGREEEQ
1560 1570 1580 1590 1600
DDIVSADVEE VEVVEGVAGE EDHHDEQEEH GEENAEAEGH HDEHDEDGSD
1610 1620 1630 1640 1650
MELDLLAAAE TESDSESNHS NQDNASGRRS VVTAATAGSE AGASSVPAFF
1660 1670 1680 1690 1700
SEDDSQSNDS SDSDSSSSQS DDIEQETFML DEPLERTTNS SHANGAAQAP
1710 1720 1730 1740 1750
RSMQWAVRNT QHQRAASTAP SSTSTPAASS AGLIYIDPSN LRRSGTISTS
1760 1770 1780 1790 1800
AAAAAAALEA SNASSYLTSA SSLARAYSIV IRQISDLMGL IPKYNHLVYS
1810 1820 1830 1840 1850
QIPAAVKLTY QDAVNLQNYV EEKLIPTWNW MVSIMDSTEA QLRYGSALAS
1860 1870 1880 1890 1900
AGDPGHPNHP LHASQNSARR ERMTAREEAS LRTLEGRRRR ATLLSARQGM
1910 1920 1930 1940 1950
MSARGDFLNY ALSLMRSHND EHSDVLPVLD VCSLKHVAYV FQALIYWIKA
1960 1970 1980 1990 2000
MNQQTTLDTP QLERKRTREL LELGIDNEDS EHENDDDTSQ SATLNDKDDE
2010 2020 2030 2040 2050
SLPAETGQNH PFFRRSDSMT FLGCIPPNPF EVPLAEAIPL ADQPHLLQPN
2060 2070 2080 2090 2100
ARKEDLFGRP SQGLYSSSAG SGKCLVEVTM DRNCLEVLPT KMSYAANLKN
2110 2120 2130 2140 2150
VMNMQNRQKK AGEDQSMLAE EADSSKPGPS AHDVAAQLKS SLLAEIGLTE
2160 2170 2180 2190 2200
SEGPPLTSFR PQCSFMGMVI SHDMLLGRWR LSLELFGRVF MEDVGAEPGS
2210 2220 2230 2240 2250
ILTELGGFEV KESKFRREME KLRNQQSRDL SLEVDRDRDL LIQQTMRQLN
2260 2270 2280 2290 2300
NHFGRRCATT PMAVHRVKVT FKDEPGEGSG VARSFYTAIA QAFLSNEKLP
2310 2320 2330 2340 2350
NLDCIQNANK GTHTSLMQRL RNRGERDRER EREREMRRSS GLRAGSRRDR
2360 2370 2380 2390 2400
DRDFRRQLSI DTRPFRPASE GNPSDDPDPL PAHRQALGER LYPRVQAMQP
2410 2420 2430 2440 2450
AFASKITGML LELSPAQLLL LLASEDSLRA RVEEAMELIV AHGRENGADS
2460 2470 2480 2490 2500
ILDLGLLDSS EKVQENRKRH GSSRSVVDMD LDDTDDGDDN APLFYQPGKR
2510 2520 2530 2540 2550
GFYTPRPGKN TEARLNCFRN IGRILGLCLL QNELCPITLN RHVIKVLLGR
2560 2570 2580 2590 2600
KVNWHDFAFF DPVMYESLRQ LILASQSSDA DAVFSAMDLA FAVDLCKEEG
2610 2620 2630 2640 2650
GGQVELIPNG VNIPVTPQNV YEYVRKYAEH RMLVVAEQPL HAMRKGLLDV
2660 2670 2680 2690 2700
LPKNSLEDLT AEDFRLLVNG CGEVNVQMLI SFTSFNDESG ENAEKLLQFK
2710 2720 2730 2740 2750
RWFWSIVERM SMTERQDLVY FWTSSPSLPA SEEGFQPMPS ITIRPPDDQH
2760 2770 2780
LPTANTCISR LYVPLYSSKQ ILKQKLLLAI KTKNFGFV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH9KVE3 | H9KVE3_RAT | E3 ubiquitin-protein ligase UBR5 | Ubr5 | 2,430 | Annotation score: |
Sequence cautioni
The sequence CAA45756 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1890 | Missing in CAA45756 (PubMed:1533713).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64411 mRNA Translation: CAA45756.1 Frameshift. |
PIRi | S22659 |
Genome annotation databases
UCSCi | RGD:621236, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64411 mRNA Translation: CAA45756.1 Frameshift. |
PIRi | S22659 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3NTW | X-ray | 2.60 | A/C | 2383-2442 | [»] | |
SMRi | Q62671 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
ELMi | Q62671 |
IntActi | Q62671, 1 interactor |
STRINGi | 10116.ENSRNOP00000009115 |
PTM databases
iPTMneti | Q62671 |
PhosphoSitePlusi | Q62671 |
Proteomic databases
jPOSTi | Q62671 |
PaxDbi | Q62671 |
PRIDEi | Q62671 |
Genome annotation databases
UCSCi | RGD:621236, rat |
Organism-specific databases
RGDi | 621236, Ubr5 |
Phylogenomic databases
eggNOGi | KOG0943, Eukaryota |
InParanoidi | Q62671 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
PROi | PR:Q62671 |
Family and domain databases
CDDi | cd14423, CUE_UBR5, 1 hit |
InterProi | View protein in InterPro IPR024725, E3_UbLigase_EDD_UBA IPR000569, HECT_dom IPR035983, Hect_E3_ubiquitin_ligase IPR036053, PABP-dom IPR002004, PABP_HYD IPR009091, RCC1/BLIP-II IPR003126, Znf_UBR |
Pfami | View protein in Pfam PF11547, E3_UbLigase_EDD, 1 hit PF00632, HECT, 1 hit PF00658, PABP, 1 hit |
SMARTi | View protein in SMART SM00119, HECTc, 1 hit SM00517, PolyA, 1 hit SM00396, ZnF_UBR1, 1 hit |
SUPFAMi | SSF50985, SSF50985, 1 hit SSF56204, SSF56204, 1 hit SSF63570, SSF63570, 1 hit |
PROSITEi | View protein in PROSITE PS50237, HECT, 1 hit PS51309, PABC, 1 hit PS51157, ZF_UBR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBR5_RAT | |
Accessioni | Q62671Primary (citable) accession number: Q62671 Secondary accession number(s): F1LRS0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 22, 2012 | |
Last modified: | December 2, 2020 | |
This is version 146 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references