We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - Q62666 (VACHT_RAT)
Protein
Vesicular acetylcholine transporter
Gene
Slc18a3
Organism
Rattus norvegicus (Rat)
Status
Functioni
Involved in acetylcholine transport into synaptic vesicles.
1 PublicationGO - Molecular functioni
- acetylcholine transmembrane transporter activity Source: RGD
- transmembrane transporter activity Source: GO_Central
GO - Biological processi
- acetylcholine transport Source: RGD
- chemical synaptic transmission Source: GO_Central
- neurotransmitter loading into synaptic vesicle Source: SynGO
Keywordsi
Biological process | Neurotransmitter transport, Transport |
Enzyme and pathway databases
Reactomei | R-RNO-264642, Acetylcholine Neurotransmitter Release Cycle R-RNO-8856825, Cargo recognition for clathrin-mediated endocytosis R-RNO-8856828, Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: Vesicular acetylcholine transporterShort name: VAChT Short name: rVAT Alternative name(s): Solute carrier family 18 member 3 |
Gene namesi | Name:Slc18a3 Synonyms:Vacht, Vat |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 62072, Slc18a3 |
Subcellular locationi
Other locations
- Membrane Sequence analysis; Multi-pass membrane protein Sequence analysis
Golgi apparatus
- AP-1 adaptor complex Source: RGD
Plasma Membrane
- AP-2 adaptor complex Source: RGD
Other locations
- axon Source: RGD
- axon terminus Source: RGD
- cholinergic synapse Source: SynGO
- cytoplasm Source: RGD
- cytoplasmic vesicle Source: RGD
- integral component of synaptic vesicle membrane Source: SynGO
- neuromuscular junction Source: SynGO
- neuron projection Source: RGD
- synaptic vesicle Source: RGD
- terminal bouton Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 33 | CytoplasmicSequence analysisAdd BLAST | 33 | |
Transmembranei | 34 – 54 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 55 – 125 | Lumenal, vesicleSequence analysisAdd BLAST | 71 | |
Transmembranei | 126 – 146 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 147 – 152 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 153 – 173 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 174 – 182 | Lumenal, vesicleSequence analysis | 9 | |
Transmembranei | 183 – 203 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 204 – 213 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 214 – 234 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 235 – 242 | Lumenal, vesicleSequence analysis | 8 | |
Transmembranei | 243 – 263 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 264 – 289 | CytoplasmicSequence analysisAdd BLAST | 26 | |
Transmembranei | 290 – 310 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 311 – 325 | Lumenal, vesicleSequence analysisAdd BLAST | 15 | |
Transmembranei | 326 – 346 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 347 – 356 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 357 – 377 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 378 – 388 | Lumenal, vesicleSequence analysisAdd BLAST | 11 | |
Transmembranei | 389 – 409 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 410 – 422 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 423 – 443 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 444 – 447 | Lumenal, vesicleSequence analysis | 4 | |
Transmembranei | 448 – 468 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 469 – 530 | CytoplasmicSequence analysisAdd BLAST | 62 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127520 | 1 – 530 | Vesicular acetylcholine transporterAdd BLAST | 530 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 89 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 96 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinPTM databases
GlyGeni | Q62666, 2 sites |
iPTMneti | Q62666 |
PhosphoSitePlusi | Q62666 |
Expressioni
Tissue specificityi
High expression in all major cholinergic cell groups, including peripheral postganglionic parasympathetic cells, preganglionic sympathetic and parasympathetic cells, ventral spinal cord and brainstem motoneurons, cell groups in the basal forebrain, the habenula and the corpus striatum. Weakly expressed in the cortex and hippocampus.3 Publications
Interactioni
Subunit structurei
Interacts with SEC14L1.
By similarityChemistry databases
BindingDBi | Q62666 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 471 – 530 | Mediates interaction with SEC14L1By similarityAdd BLAST | 60 | |
Regioni | 504 – 530 | DisorderedSequence analysisAdd BLAST | 27 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
InParanoidi | Q62666 |
OrthoDBi | 956763at2759 |
PhylomeDBi | Q62666 |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q62666-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEPTAPTGQA RAAATKLSEA VGAALQEPQR QRRLVLVIVC VALLLDNMLY
60 70 80 90 100
MVIVPIVPDY IAHMRGGSEG PTLVSEVWEP TLPPPTLANA SAYLANTSAS
110 120 130 140 150
PTAAGSARSI LRPRYPTESE DVKIGVLFAS KAILQLLVNP LSGPFIDRMS
160 170 180 190 200
YDVPLLIGLG VMFASTVMFA FAEDYATLFA ARSLQGLGSA FADTSGIAMI
210 220 230 240 250
ADKYPEEPER SRALGVALAF ISFGSLVAPP FGGILYEFAG KRVPFLVLAA
260 270 280 290 300
VSLFDALLLL AVAKPFSAAA RARANLPVGT PIHRLMLDPY IAVVAGALTT
310 320 330 340 350
CNIPLAFLEP TIATWMKHTM AASEWEMGMV WLPAFVPHVL GVYLTVRLAA
360 370 380 390 400
RYPHLQWLYG ALGLAVIGVS SCVVPACRSF APLVVSLCGL CFGIALVDTA
410 420 430 440 450
LLPTLAFLVD VRHVSVYGSV YAIADISYSV AYALGPIVAG HIVHSLGFEQ
460 470 480 490 500
LSLGMGLANL LYAPVLLLLR NVGLLTRSRS ERDVLLDEPP QGLYDAVRLR
510 520 530
EVQGKDGGEP CSPPGPFDGC EDDYNYYSRS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1B0GWU4 | A0A1B0GWU4_RAT | Solute carrier family 18 (Vesicular... | Slc18a3 rCG_42278 | 530 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 92 | A → T in AAA50831 (PubMed:7938002).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09838 mRNA Translation: AAA50831.1 U09211 mRNA Translation: AAA20498.1 X80395 Genomic DNA Translation: CAA56604.1 |
RefSeqi | NP_113851.1, NM_031663.2 |
Genome annotation databases
GeneIDi | 60422 |
KEGGi | rno:60422 |
UCSCi | RGD:62072, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09838 mRNA Translation: AAA50831.1 U09211 mRNA Translation: AAA20498.1 X80395 Genomic DNA Translation: CAA56604.1 |
RefSeqi | NP_113851.1, NM_031663.2 |
3D structure databases
SMRi | Q62666 |
ModBasei | Search... |
Chemistry databases
BindingDBi | Q62666 |
ChEMBLi | CHEMBL2125 |
DrugCentrali | Q62666 |
PTM databases
GlyGeni | Q62666, 2 sites |
iPTMneti | Q62666 |
PhosphoSitePlusi | Q62666 |
Protocols and materials databases
ABCDi | Q62666, 2 sequenced antibodies |
Genome annotation databases
GeneIDi | 60422 |
KEGGi | rno:60422 |
UCSCi | RGD:62072, rat |
Organism-specific databases
CTDi | 6572 |
RGDi | 62072, Slc18a3 |
Phylogenomic databases
InParanoidi | Q62666 |
OrthoDBi | 956763at2759 |
PhylomeDBi | Q62666 |
Enzyme and pathway databases
Reactomei | R-RNO-264642, Acetylcholine Neurotransmitter Release Cycle R-RNO-8856825, Cargo recognition for clathrin-mediated endocytosis R-RNO-8856828, Clathrin-mediated endocytosis |
Miscellaneous databases
PROi | PR:Q62666 |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VACHT_RAT | |
Accessioni | Q62666Primary (citable) accession number: Q62666 Secondary accession number(s): Q64351 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2003 |
Last sequence update: | September 19, 2003 | |
Last modified: | February 23, 2022 | |
This is version 125 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families