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Protein

Transcription factor 4

Gene

Tcf4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Interacts with the CCAAT displacement protein (CDP2) to bind the tyrosine hydroxylase enhancer.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor 4
Short name:
TCF-4
Alternative name(s):
Immunoglobulin transcription factor 2
Short name:
ITF-2
Short name:
RITF-2
R8f DNA-binding protein
SL3-3 enhancer factor 2
Short name:
SEF-2
Gene namesi
Name:Tcf4
Synonyms:Itf2, Sef2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69271 Tcf4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001272581 – 589Transcription factor 4Add BLAST589

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphoserineBy similarity1
Modified residuei13PhosphoserineBy similarity1
Modified residuei290PhosphoserineCombined sources1
Modified residuei433PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ62655
PRIDEiQ62655

PTM databases

iPTMnetiQ62655
PhosphoSitePlusiQ62655

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin. Interacts with HIVEP2. Interacts with NEUROD2 (By similarity). Interacts with AGBL1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

CORUMiQ62655
STRINGi10116.ENSRNOP00000020431

Structurei

3D structure databases

ProteinModelPortaliQ62655
SMRiQ62655
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini486 – 539bHLHPROSITE-ProRule annotationAdd BLAST54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni136 – 157Leucine-zipperAdd BLAST22
Regioni380 – 403Class A specific domainAdd BLAST24

Phylogenomic databases

eggNOGiKOG3910 Eukaryota
ENOG410XYUA LUCA
HOGENOMiHOG000234180
HOVERGENiHBG003854
InParanoidiQ62655
KOiK15603
PhylomeDBiQ62655

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q62655-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTSRDLGSHD NLSPPFANSR IQSKTERGSY SSYGRENVQG CHQSLLGGDM
60 70 80 90 100
DMGNPGTLSP TKPGSQYYPY SSNNARRRPL HSSTMEVQTK KVRKVPPGLP
110 120 130 140 150
SSVYAPSAST ADYNRDSPGY SSSKPAASTF SSSFFMQDGH HSSDPWSSSS
160 170 180 190 200
GMNQPGYGGM LGNSHIPQSS SYCSLHPHER LSYPSHSSAD INSSLPPMST
210 220 230 240 250
FHRSGTNHYS TSSCTPPANG TDSIMANRGT GAAGSSQTGD ALGKALASIY
260 270 280 290 300
SPDHTNNSFS SNPSTPVGSP PSLSAGTAVW SRNGGQASSS PNYEGPLHSL
310 320 330 340 350
QSRIEDRLER LDDAIHVLRN HAVGPSTAVP GGHGDMHGII GPSHNGAMGS
360 370 380 390 400
LGSGYGTGLL SANRHSLMVG AHREDGVALR GSHSLLPNQV PVPQLPVQSA
410 420 430 440 450
TSPDLNPPQD PYRGMPPGLQ GQSVSSGSSE IKSDDEGDEN LQDTKSSEDK
460 470 480 490 500
KLDDDKKDIK SITRSRSSNN DDEDLTPEQK AEREKERRMA NNARERLRVR
510 520 530 540 550
DINEAFKELG RMVQLHLKSD KPQTKLLILH QAVAVILSLE QQVRERNLNP
560 570 580
KAACLKRREE EKVSSEPPPL SLAGPHPGMG DTANHMGQM
Note: No experimental confirmation available.
Length:589
Mass (Da):63,053
Last modified:December 6, 2005 - v2
Checksum:iD2A271D197D1058C
GO
Isoform 2 (identifier: Q62655-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     464-467: Missing.

Show »
Length:585
Mass (Da):62,567
Checksum:i2DEF03D3508F3CE7
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JWU7A0A0G2JWU7_RAT
Transcription factor 4
Tcf4 rCG_46972
669Annotation score:
F1LQ90F1LQ90_RAT
Transcription factor 4
Tcf4
585Annotation score:
A0A0G2JXN5A0A0G2JXN5_RAT
Transcription factor 4
Tcf4
548Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti582T → A in AAA21122 (PubMed:7913462).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002116464 – 467Missing in isoform 2. 2 Publications4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149284 mRNA Translation: AAK26670.1
U09228 mRNA Translation: AAA21122.1
PIRiA53689
RefSeqiNP_445821.1, NM_053369.1 [Q62655-2]
UniGeneiRn.23354

Genome annotation databases

GeneIDi84382
KEGGirno:84382

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149284 mRNA Translation: AAK26670.1
U09228 mRNA Translation: AAA21122.1
PIRiA53689
RefSeqiNP_445821.1, NM_053369.1 [Q62655-2]
UniGeneiRn.23354

3D structure databases

ProteinModelPortaliQ62655
SMRiQ62655
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiQ62655
STRINGi10116.ENSRNOP00000020431

PTM databases

iPTMnetiQ62655
PhosphoSitePlusiQ62655

Proteomic databases

PaxDbiQ62655
PRIDEiQ62655

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi84382
KEGGirno:84382

Organism-specific databases

CTDi6925
RGDi69271 Tcf4

Phylogenomic databases

eggNOGiKOG3910 Eukaryota
ENOG410XYUA LUCA
HOGENOMiHOG000234180
HOVERGENiHBG003854
InParanoidiQ62655
KOiK15603
PhylomeDBiQ62655

Miscellaneous databases

PROiPR:Q62655

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiITF2_RAT
AccessioniPrimary (citable) accession number: Q62655
Secondary accession number(s): Q99N33
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 6, 2005
Last modified: September 12, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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